目的:探讨乌司他丁(u linastatin,UTI)对缺血再灌注(IR)损伤大鼠肠黏膜免疫屏障的保护作用及机制。方法:采用大鼠小肠缺血45 m in再灌注模型,治疗组于再灌注5 m in内尾静脉缓慢注射UTI。于再灌注后2 h用免疫放射法测定血清、肠黏膜组织...目的:探讨乌司他丁(u linastatin,UTI)对缺血再灌注(IR)损伤大鼠肠黏膜免疫屏障的保护作用及机制。方法:采用大鼠小肠缺血45 m in再灌注模型,治疗组于再灌注5 m in内尾静脉缓慢注射UTI。于再灌注后2 h用免疫放射法测定血清、肠黏膜组织中分泌型免疫球蛋白A(sIgA)的含量;四甲基偶氮唑盐微量酶反应比色法(MTT)测定Peyer's淋巴结(pp)、上皮内淋巴细胞(IEL)和固有层淋巴细胞(LPL)增殖活力。结果:肠IR后血清和肠黏膜中sIgA含量下降,淋巴细胞增殖活性下降(P<0.01);不同剂量的UTI治疗组均能逆转IR后sIgA含量下降和淋巴细胞增殖活性下降,差异有显著意义(P<0.01),而不同剂量UTI治疗组间各项指标也有显著差异(P<0.05)。结论:乌司他丁(UTI)可参与调节肠道相关淋巴细胞免疫功能,对缺血再灌注损伤肠道免疫屏障具有保护作用。展开更多
The gastrointestinal tract uses a system of tolerance and controlled inflammation to limit the response to dietary or bacteria-derived antigens in the gut. When this complex system breaks down, either by a chemi- cal ...The gastrointestinal tract uses a system of tolerance and controlled inflammation to limit the response to dietary or bacteria-derived antigens in the gut. When this complex system breaks down, either by a chemi- cal or pathogenic insult in a genetically predisposed individual the resulting immune response may lead to inflammatory bowel disease. Although the aetio- pathogenesis of inflammatory bowel disease remains unsolved current evidence indicates that defective T-cell apoptosis and impairment of intestinal epithelial barrier function play important roles. In inflammatory bowel disease, it has been reported that activation of macrophages seems to be as important as increased production of the macrophage-derived cytokines such as TNF-α, IL-1 and IL-6. The triggering factor for this cascade is still to be elucidated as to whether it rep- resents an auto-antigen or a hetero-antigen. It has been also demonstrated that a serologic anti-microbial response exists. This response includes antibodies against saccharomyces cerevisiae （ASCA）, E. coli outer membrane porin C （Omp-C）, flagelin （cBirl） and pseu- domonas aeroginosa （I2）. Host response to microbial pathogens includes self-defense mechanisms including defensins, pattern recognition receptors and Toll-like receptors. Neuroimmunomodulation in inflammatory bowel disease （IBD） is another interesting approach with implications on the influence of brain-gut axis on intestinal inflammation and its perpetuation. It isprobable that inflammatory bowel disease represents a heterogenic group of diseases that share similar mechanisms of tissue damage but have different ini- tiating events and immunoregulatory abnormalities. A better understanding of all these events will hope- fully provide new insights into the mechanisms of epithelial responses to microorganisms and ideas for therapies.展开更多
Sympathetic system modulation by stellate ganglion blockade may modulate immune dysfunction and significantly improve symptoms of chronic ulcerative colitis.
AIM： To explore the effect of Gui Zhi decoction on enteric mucosal immune in type Ⅱ collagen-induced arthritis （CIA） in DBA mice. METHODS： Eighty DBA/1, weighing 18-22 g, were randomly divided into four groups wi...AIM： To explore the effect of Gui Zhi decoction on enteric mucosal immune in type Ⅱ collagen-induced arthritis （CIA） in DBA mice. METHODS： Eighty DBA/1, weighing 18-22 g, were randomly divided into four groups with 20 in each group： control group, CIA group, treatment groups at high dosage and low dosage （GZH and GZL）. CIA was induced by immunization with type Ⅱ collagen （CII） emulsified with equal complete adjuvant at 0.1 mg CII each mouse. Blood lymphocyte suspension was screened for CD4 and CD8 expression using a flow cytometry, the CD4 and CD8 and secretory IgA （sIgA）-positive cells in enteric lamina propria tested with immunohistochemical staining. Tumor necrosis factor-alpha （TNF-α）, interleukin-1 （IL-1）-β, and IL-6 concentrations in serum were assayed with RIA. RESULTS： Gui Zhi decoction can lower the arthritic scores and decrease the occurrence of arthritis. The CD4, CD8, and sIgA-positive cells in CIA mice are less than in control mice, and in Gui Zhi decoction at high dosage could restore the lowered CD4- and CD8-positive cells in lamina propria, and at both high and low dosages could increase the lowered sIgA-positive cells in lamina propria, even still lower than in normal mice. In periphery, the CD4 cells in periphery are higher in CIA mice than in control mice, and Gui Zhi decoction at high and low dosages could decrease the CD4 and CD8 cells. Also, Gui Zhi decoction at high dosage could decrease the IL-6 and TNF-α concentration in serum. CONCLUSION： Gui Zhi decoction can lower the arthritic scores and decrease the incidence of CIA in mice, and the mechanism is in part regulating enteric mucosal immune.展开更多
AIM:Many defense factors of the mother's colostrum or milk protect infants from intestinal, respiratory and systemic infections. In the present study, we investigated the effect of colostrum and mature human milk ...AIM:Many defense factors of the mother's colostrum or milk protect infants from intestinal, respiratory and systemic infections. In the present study, we investigated the effect of colostrum and mature human milk on E. histolytica parasites in vitro.METHODS:Samples of human milk were collected from 5 healthy lactating mothers.The medium with human milk at concentrations of 2%, 5% and 10% was obtained.RESULTS:The lethal effect of E. histolytica on the medium supplemented with different concentrations of both colostrum and mature human milk was significant during the first 30min. We also detected that the results of colostrum and mature human milk were similar. No statistically significant differences were found between same concentrations of colostrum and mature human milk at the same times.CONCLUSION:Colostrum and mature human milk have significant lethal effect on E. histolytica and protect against its infection in breast fed children.展开更多
The human gut is the normal habitat of a lot of microorganisms; in the long process of evolution they have developed a symbiotic relationship with the host. Under normal circumstances they do not damage the health, wh...The human gut is the normal habitat of a lot of microorganisms; in the long process of evolution they have developed a symbiotic relationship with the host. Under normal circumstances they do not damage the health, which totally owes to the barrier functions of the integral intestinal mucosa. Intestinal lnucosal barriers are mainly composed of four parts： mechanical barrier, immune barrier, chemical and biological barrier. Each of these functions has corresponding structural basis, an important barrier to prevent harmful substances and pathogens in the gut into the inner environment of the body, and to maintain a stable internal environment.展开更多
文摘目的:探讨乌司他丁(u linastatin,UTI)对缺血再灌注(IR)损伤大鼠肠黏膜免疫屏障的保护作用及机制。方法:采用大鼠小肠缺血45 m in再灌注模型,治疗组于再灌注5 m in内尾静脉缓慢注射UTI。于再灌注后2 h用免疫放射法测定血清、肠黏膜组织中分泌型免疫球蛋白A(sIgA)的含量;四甲基偶氮唑盐微量酶反应比色法(MTT)测定Peyer's淋巴结(pp)、上皮内淋巴细胞(IEL)和固有层淋巴细胞(LPL)增殖活力。结果:肠IR后血清和肠黏膜中sIgA含量下降,淋巴细胞增殖活性下降(P<0.01);不同剂量的UTI治疗组均能逆转IR后sIgA含量下降和淋巴细胞增殖活性下降,差异有显著意义(P<0.01),而不同剂量UTI治疗组间各项指标也有显著差异(P<0.05)。结论:乌司他丁(UTI)可参与调节肠道相关淋巴细胞免疫功能,对缺血再灌注损伤肠道免疫屏障具有保护作用。
文摘The gastrointestinal tract uses a system of tolerance and controlled inflammation to limit the response to dietary or bacteria-derived antigens in the gut. When this complex system breaks down, either by a chemi- cal or pathogenic insult in a genetically predisposed individual the resulting immune response may lead to inflammatory bowel disease. Although the aetio- pathogenesis of inflammatory bowel disease remains unsolved current evidence indicates that defective T-cell apoptosis and impairment of intestinal epithelial barrier function play important roles. In inflammatory bowel disease, it has been reported that activation of macrophages seems to be as important as increased production of the macrophage-derived cytokines such as TNF-α, IL-1 and IL-6. The triggering factor for this cascade is still to be elucidated as to whether it rep- resents an auto-antigen or a hetero-antigen. It has been also demonstrated that a serologic anti-microbial response exists. This response includes antibodies against saccharomyces cerevisiae （ASCA）, E. coli outer membrane porin C （Omp-C）, flagelin （cBirl） and pseu- domonas aeroginosa （I2）. Host response to microbial pathogens includes self-defense mechanisms including defensins, pattern recognition receptors and Toll-like receptors. Neuroimmunomodulation in inflammatory bowel disease （IBD） is another interesting approach with implications on the influence of brain-gut axis on intestinal inflammation and its perpetuation. It isprobable that inflammatory bowel disease represents a heterogenic group of diseases that share similar mechanisms of tissue damage but have different ini- tiating events and immunoregulatory abnormalities. A better understanding of all these events will hope- fully provide new insights into the mechanisms of epithelial responses to microorganisms and ideas for therapies.
文摘Sympathetic system modulation by stellate ganglion blockade may modulate immune dysfunction and significantly improve symptoms of chronic ulcerative colitis.
基金Supported by the project of National Natural Science Foundation of China, No. 30171133 and 39870952
文摘AIM： To explore the effect of Gui Zhi decoction on enteric mucosal immune in type Ⅱ collagen-induced arthritis （CIA） in DBA mice. METHODS： Eighty DBA/1, weighing 18-22 g, were randomly divided into four groups with 20 in each group： control group, CIA group, treatment groups at high dosage and low dosage （GZH and GZL）. CIA was induced by immunization with type Ⅱ collagen （CII） emulsified with equal complete adjuvant at 0.1 mg CII each mouse. Blood lymphocyte suspension was screened for CD4 and CD8 expression using a flow cytometry, the CD4 and CD8 and secretory IgA （sIgA）-positive cells in enteric lamina propria tested with immunohistochemical staining. Tumor necrosis factor-alpha （TNF-α）, interleukin-1 （IL-1）-β, and IL-6 concentrations in serum were assayed with RIA. RESULTS： Gui Zhi decoction can lower the arthritic scores and decrease the occurrence of arthritis. The CD4, CD8, and sIgA-positive cells in CIA mice are less than in control mice, and in Gui Zhi decoction at high dosage could restore the lowered CD4- and CD8-positive cells in lamina propria, and at both high and low dosages could increase the lowered sIgA-positive cells in lamina propria, even still lower than in normal mice. In periphery, the CD4 cells in periphery are higher in CIA mice than in control mice, and Gui Zhi decoction at high and low dosages could decrease the CD4 and CD8 cells. Also, Gui Zhi decoction at high dosage could decrease the IL-6 and TNF-α concentration in serum. CONCLUSION： Gui Zhi decoction can lower the arthritic scores and decrease the incidence of CIA in mice, and the mechanism is in part regulating enteric mucosal immune.
文摘AIM:Many defense factors of the mother's colostrum or milk protect infants from intestinal, respiratory and systemic infections. In the present study, we investigated the effect of colostrum and mature human milk on E. histolytica parasites in vitro.METHODS:Samples of human milk were collected from 5 healthy lactating mothers.The medium with human milk at concentrations of 2%, 5% and 10% was obtained.RESULTS:The lethal effect of E. histolytica on the medium supplemented with different concentrations of both colostrum and mature human milk was significant during the first 30min. We also detected that the results of colostrum and mature human milk were similar. No statistically significant differences were found between same concentrations of colostrum and mature human milk at the same times.CONCLUSION:Colostrum and mature human milk have significant lethal effect on E. histolytica and protect against its infection in breast fed children.
文摘The human gut is the normal habitat of a lot of microorganisms; in the long process of evolution they have developed a symbiotic relationship with the host. Under normal circumstances they do not damage the health, which totally owes to the barrier functions of the integral intestinal mucosa. Intestinal lnucosal barriers are mainly composed of four parts： mechanical barrier, immune barrier, chemical and biological barrier. Each of these functions has corresponding structural basis, an important barrier to prevent harmful substances and pathogens in the gut into the inner environment of the body, and to maintain a stable internal environment.
