Objective：To observe the effect of electroacupuncture （EA） on the expressions of colonic nerve growth factor （NGF） and nerve growth factor receptor （NGFR） in visceral hypersensitivity rats, and investigate the ...Objective：To observe the effect of electroacupuncture （EA） on the expressions of colonic nerve growth factor （NGF） and nerve growth factor receptor （NGFR） in visceral hypersensitivity rats, and investigate the mechanisms of EA on chronic visceral hypersensitivity. Methods： A total of 24 neonatal rats were randomly divided into a normal group, a model group and an EA group. The models of visceral hypersensitivity rats were prepared according to the method of Al-Chaer. The rats in the EA group were treated with EA at Tianshu （ST 25） and Shangjuxu （ST 37）, 20 min for each time, once a day, for 7 consecutive days. After the first treatment, the observation of abdominal withdrawal reflex （AWR） was used to evaluate the pain threshold of visceral hypersensitivity rats. After 7-day treatments, the colonic tissue of rat was collected to test the immunohistochemistry of NGF and NGFR. Results： The score of AWR in the model group was higher than that in the normal group, but the score decreased after EA treatment. The positive expressions of colonic NGF and NGFR in the model group were increased than those in the normal group （P〈0.05）, while they were both decreased in the EA group after treatment （P〈0.001）. Conclusion： The EA could up-regulate the pain threshold of visceral hypersensitivity rats, and down-regulate the expressions of colonic NGF and NGFR. The regulation of the expressions of colonic NGF and NGFR would be one of the peripheral mechanisms of EA in treating chronic visceral hypersensitivity.展开更多
Objective To observe the therapeutic effect of mild moxibustion on irritable bowel syndrome(IBS)visceral hyperalgesia model rats and its regulatory effect on P2X3 receptors in the spinal cord,anterior cingutate cortex...Objective To observe the therapeutic effect of mild moxibustion on irritable bowel syndrome(IBS)visceral hyperalgesia model rats and its regulatory effect on P2X3 receptors in the spinal cord,anterior cingutate cortex(ACC)and thalamic ventral posterolateral nucleus(VPL).Methods Thirty 8-day-old newborn rats were randomly divided into a normal group(n=6)and a modeling group(n=24)according to the completely random number table method.Rats in the normal group were bred routinely,and those in the modeling group were subjected to preparing IBS chronic visceral hyperalgesia model using colorectal distention(CRD)in stimulation method.Rats successfully modelled were re-divided into a model group,a mild moxibustion group,a P2X3 receptor antagonist group,and a normal saline group according to the completely random number table method with 6 rats in each group.Rats in each group received corresponding interventions from the 37-day old,once a day for 7 consecutive days.Immunohistochemistry and Western blot assays were used to detect P2X3 protein expressions in the spinal cord,ACC and VPL of rats.Results Under different intensities of CRD stimulation,the abdominal withdrawal reflex(AWR)scores of the model group were significantly increased versus the normal group(all P<0.05);the AWR scores of the mild moxibustion group and the P2X3 receptor antagonist group were significantly reduced versus the model group(all P<0.01).The P2X3 protein expressions in rat spinal cord,ACC and VPL tissues of the model group were significantly increased versus the normal group(all P<0.01);the P2X3 protein expressions in rat spinal cord,ACC and VPL tissues of the mild moxibustion group and the P2X3 receptor antagonist group were significantly reduced versus the model group(all P<0.01).Conclusion Mild moxibustion can inhibit the P2X3 receptor expressions in the spinal cord,ACC,and VPL tissues of IBS visceral hyperalgesia model rats,which may be the mechanism of mild moxibustion in relieving the central sensitization of rats with IBS visceral hyperalgesia.展开更多
基金supported by Project of Shanghai Natural Sciences Foundation,No.14ZR1438700National Basic Research Program of China (973 Program),No.2015CB554500+1 种基金The Second Period Xinglin Scholars Program of Shanghai University of Traditional Chinese MedicineNational Natural Sciences Foundation of China,No.81202752~~
文摘Objective：To observe the effect of electroacupuncture （EA） on the expressions of colonic nerve growth factor （NGF） and nerve growth factor receptor （NGFR） in visceral hypersensitivity rats, and investigate the mechanisms of EA on chronic visceral hypersensitivity. Methods： A total of 24 neonatal rats were randomly divided into a normal group, a model group and an EA group. The models of visceral hypersensitivity rats were prepared according to the method of Al-Chaer. The rats in the EA group were treated with EA at Tianshu （ST 25） and Shangjuxu （ST 37）, 20 min for each time, once a day, for 7 consecutive days. After the first treatment, the observation of abdominal withdrawal reflex （AWR） was used to evaluate the pain threshold of visceral hypersensitivity rats. After 7-day treatments, the colonic tissue of rat was collected to test the immunohistochemistry of NGF and NGFR. Results： The score of AWR in the model group was higher than that in the normal group, but the score decreased after EA treatment. The positive expressions of colonic NGF and NGFR in the model group were increased than those in the normal group （P〈0.05）, while they were both decreased in the EA group after treatment （P〈0.001）. Conclusion： The EA could up-regulate the pain threshold of visceral hypersensitivity rats, and down-regulate the expressions of colonic NGF and NGFR. The regulation of the expressions of colonic NGF and NGFR would be one of the peripheral mechanisms of EA in treating chronic visceral hypersensitivity.
文摘Objective To observe the therapeutic effect of mild moxibustion on irritable bowel syndrome(IBS)visceral hyperalgesia model rats and its regulatory effect on P2X3 receptors in the spinal cord,anterior cingutate cortex(ACC)and thalamic ventral posterolateral nucleus(VPL).Methods Thirty 8-day-old newborn rats were randomly divided into a normal group(n=6)and a modeling group(n=24)according to the completely random number table method.Rats in the normal group were bred routinely,and those in the modeling group were subjected to preparing IBS chronic visceral hyperalgesia model using colorectal distention(CRD)in stimulation method.Rats successfully modelled were re-divided into a model group,a mild moxibustion group,a P2X3 receptor antagonist group,and a normal saline group according to the completely random number table method with 6 rats in each group.Rats in each group received corresponding interventions from the 37-day old,once a day for 7 consecutive days.Immunohistochemistry and Western blot assays were used to detect P2X3 protein expressions in the spinal cord,ACC and VPL of rats.Results Under different intensities of CRD stimulation,the abdominal withdrawal reflex(AWR)scores of the model group were significantly increased versus the normal group(all P<0.05);the AWR scores of the mild moxibustion group and the P2X3 receptor antagonist group were significantly reduced versus the model group(all P<0.01).The P2X3 protein expressions in rat spinal cord,ACC and VPL tissues of the model group were significantly increased versus the normal group(all P<0.01);the P2X3 protein expressions in rat spinal cord,ACC and VPL tissues of the mild moxibustion group and the P2X3 receptor antagonist group were significantly reduced versus the model group(all P<0.01).Conclusion Mild moxibustion can inhibit the P2X3 receptor expressions in the spinal cord,ACC,and VPL tissues of IBS visceral hyperalgesia model rats,which may be the mechanism of mild moxibustion in relieving the central sensitization of rats with IBS visceral hyperalgesia.
