目的比较脉冲场凝胶电泳(pulsed field gel electrophoresis,PFGE)与肠杆菌科基因间重复序列聚合酶链式反应(enterobacterial repetitive intergenic consensus-polymerase chain reaction,ERICPCR)检测鲍曼不动杆菌同源性的结果差异。...目的比较脉冲场凝胶电泳(pulsed field gel electrophoresis,PFGE)与肠杆菌科基因间重复序列聚合酶链式反应(enterobacterial repetitive intergenic consensus-polymerase chain reaction,ERICPCR)检测鲍曼不动杆菌同源性的结果差异。方法分别采用PFGE和ERIC-PCR对我院院内分离的43株鲍曼不动杆菌进行分型检测。结果 43株鲍曼不动杆菌通过PFGE分型得出:A型22株、B型10株、C型3株、D型4株、E型2株、F型1株、G型1株;通过ERIC-PCR得出7种型别:Ⅰ型22株、Ⅱ型10株、Ⅲ型3株、Ⅳ型2株、V型3株、Ⅵ型1株、Ⅶ型2株。2种方法结果相符率为76.8%。我院鲍曼不动杆菌存在克隆株传播。结论 ERIC-PCR操作简便、结果可靠,与PFGE结果一致性高,2种分型方法均适合作为医院进行病原菌流行病学调查的分型检测手段。展开更多
Objective. An association between polymorphism in the gene coding for the anti- inflammatory cytokine interleukin- 1- receptor antagonist (IL- 1Ra) and ulcerative colitis (UC) has been reported. To date, there is no r...Objective. An association between polymorphism in the gene coding for the anti- inflammatory cytokine interleukin- 1- receptor antagonist (IL- 1Ra) and ulcerative colitis (UC) has been reported. To date, there is no report from India confirming this association. In the present study the aim was to assess the allele frequencies and carriage rates of different alleles of 86 bp (base pair) variable number tandem repeat (VNTR) in intron 2 of the IL- 1Ra gene in patients with inflammatory bowel disease (IBD) and healthy controls from northern India. Material and methods. Eighty- two patients with UC, 21 with Crohn’ s disease (CD) and 141 ethnically matched controls were enrolled in this study. Genotyping was done using a polymerase chain reaction (PCR) amplification of the intron- 2 fragment harboring a VNTR nucleotide sequence. The PCR products were separated on 2% agarose gel. Statistical analysis was performed using the chi- squared (χ 2) test. Results. The frequencies of allele 2 in UC, CD and healthy controls were 26% , 50% and 24% , respectively. The frequency of allele 2 in CD was higher than that in UC (p = 0.002; OR = 2.9) and healthy controls (p = 0.001; OR = 3.1; 95% CI = 1.5- 6.3). Alleles 3 and 4 were absent in patients with CD, while allele 5 was absent in all three groups. Conclusions. The present study demonstrated an association between allele 2 and patients with CD but not with UC. Interestingly, the allele frequency and carriage rates of allele 2 were significantly higher in patients with CD than in patients with UC and in healthy subjects. Ethnic differences, genetic heterogeneity and sample size could be the reasons for such differences in comparison with studies from the West.展开更多
文摘Objective. An association between polymorphism in the gene coding for the anti- inflammatory cytokine interleukin- 1- receptor antagonist (IL- 1Ra) and ulcerative colitis (UC) has been reported. To date, there is no report from India confirming this association. In the present study the aim was to assess the allele frequencies and carriage rates of different alleles of 86 bp (base pair) variable number tandem repeat (VNTR) in intron 2 of the IL- 1Ra gene in patients with inflammatory bowel disease (IBD) and healthy controls from northern India. Material and methods. Eighty- two patients with UC, 21 with Crohn’ s disease (CD) and 141 ethnically matched controls were enrolled in this study. Genotyping was done using a polymerase chain reaction (PCR) amplification of the intron- 2 fragment harboring a VNTR nucleotide sequence. The PCR products were separated on 2% agarose gel. Statistical analysis was performed using the chi- squared (χ 2) test. Results. The frequencies of allele 2 in UC, CD and healthy controls were 26% , 50% and 24% , respectively. The frequency of allele 2 in CD was higher than that in UC (p = 0.002; OR = 2.9) and healthy controls (p = 0.001; OR = 3.1; 95% CI = 1.5- 6.3). Alleles 3 and 4 were absent in patients with CD, while allele 5 was absent in all three groups. Conclusions. The present study demonstrated an association between allele 2 and patients with CD but not with UC. Interestingly, the allele frequency and carriage rates of allele 2 were significantly higher in patients with CD than in patients with UC and in healthy subjects. Ethnic differences, genetic heterogeneity and sample size could be the reasons for such differences in comparison with studies from the West.
