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2株番木瓜根际促生菌的解磷解钾作用 被引量:10
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作者 叶维雁 吴海波 +2 位作者 刘惠民 熊智 王樱潼 《江苏农业科学》 北大核心 2017年第6期247-251,共5页
用平板涂布、划线与固体平板-透明圈法从番木瓜根际土壤中筛选出2株解磷解钾细菌(PK-1、PK-2)。通过摇瓶培养,测定PK-1、PK-2发酵液中水溶性磷、水溶性钾含量和磷、钾总量;分析PK-1、PK-2解磷解钾能力;对PK-1、PK-2进行分子鉴定。结果表... 用平板涂布、划线与固体平板-透明圈法从番木瓜根际土壤中筛选出2株解磷解钾细菌(PK-1、PK-2)。通过摇瓶培养,测定PK-1、PK-2发酵液中水溶性磷、水溶性钾含量和磷、钾总量;分析PK-1、PK-2解磷解钾能力;对PK-1、PK-2进行分子鉴定。结果表明,PK-1溶解无机磷能力的溶量为1.63μg/m L,PK-2溶量为17.26μg/m L,溶解无机磷能力表现为PK-1﹤PK-2,即PK-2具有更强的解无机磷能力;PK-1溶解有机磷能力的溶量为8.49μg/m L,PK-2溶量为2.38μg/m L,溶解有机磷能力表现为PK-1>PK-2,即PK-1的解有机磷能力更好;PK-1溶解钾能力的溶量为8.15μg/m L,PK-2溶量为8.49μg/m L,溶解钾能力表现为PK-1﹤PK-2,PK-1和PK-2的解钾能力接近。经16S r DNA序列分析,初步鉴定PK-1为肠杆菌属(Enterobacter),PK-2为芽孢杆菌属(Bacillus)。供试菌株PK-1和PK-2具有良好的解磷解钾功能,可为进一步研制专用的番木瓜微生物肥料提供菌种资源和依据。 展开更多
关键词 番木瓜 根际促生菌 解磷 解钾 鉴定 肠杆菌层 芽孢杆菌
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Sedimentary response to volcanic activity in the Okinawa Trough since the last deglaciation 被引量:2
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作者 蒋富清 李安春 李铁刚 《Chinese Journal of Oceanology and Limnology》 SCIE CAS CSCD 2010年第1期171-182,共12页
To investigate the relationship between volcanic activity and sediment record on regional and temporal scales,158 surface sediment samples were collected from the East China Sea Shelf to the northern Okinawa Trough (O... To investigate the relationship between volcanic activity and sediment record on regional and temporal scales,158 surface sediment samples were collected from the East China Sea Shelf to the northern Okinawa Trough (OT),and two cores recovered in the northern and southern OT,respectively.Mineralogy,grain-size,and geochemical analyses of those samples show that:1) volcanic glass,volcanic-type pyroxene,hypersthenes,and magnetite increase in sediment influenced by volcanic activity;2) sediment grain sizes (and also silt content) increase in ash layers;and 3) the contents of Na2O and Zr are higher,while terrigenous elements,e.g.,TFe2O3 and K2O,and biogenous compositions,e.g.,CaO and Sr,are relatively lower in ash layers than those of non-ash layers.The distribution of volcanic ash has three distinguishing characteristics:1) volcanic ash is more abundant in the northern and central OT than the southern OT;2) volcanic ash increases from continental shelf to the trough;3) the sediment during the last 12 000 a suggests stronger volcanic events than during 15 000-12 000 a.The eruptive locations,frequency,and volume of calderas are among the most important factors controlling the distributions of volcanic ash.In addition,the main Kuroshio warm current that extends northward probably impeded the diffusion of volcanic ash to the west and south in the OT.However,a southward current probably carried some volcanic ash toward southern OT. 展开更多
关键词 SEDIMENT ash layer last deglaciation Okinawa Trough
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Reevaluation of cortical developmental patterns in Euplotes(s.l.), including a morphogenetic redescription of E.charon (Protozoa,Ciliophora,Euplotida) 被引量:4
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作者 邵晨 马洪钢 +2 位作者 高珊 AL-RASHEID A.Khaled 宋微波 《Chinese Journal of Oceanology and Limnology》 SCIE CAS CSCD 2010年第3期593-602,共10页
We documented the pattern of cell development in Euplotes charon. The ontogenesis of this species was similar to many of its congeners, except for the formation of the caudal cirri. In E. Charon, a caudal cirrus is fo... We documented the pattern of cell development in Euplotes charon. The ontogenesis of this species was similar to many of its congeners, except for the formation of the caudal cirri. In E. Charon, a caudal cirrus is formed posterior to each of the rightmost two or three dorsal kinety anlage in the proter, and the second rightmost dorsal kinety in the opisthe. In addition, two caudal cirri are formed posterior to the rightmost dorsal kinety in the opisthe. This pattern of development represents a completely new type. Based on our evaluation, and in comparison with previous studies, we also conclude that the pattern of cell development is variable among species in the Euplotes genera. The variation is particularly evident during the formation of frontoventral and caudal cirri. Based on the segmentation pattern of frontal-midventral transverse cirral anlagen, cirri reduction, and migration of frontoventral cirri, we identified five types: the affinis-type, the eurystomus-type, the charon-type, the raikovi-type and orientalis-type. Euplotes (s. l.) can also be divided into three types based on the formation of caudal cirri: focardii-type, vannus-type and charon-type. Indeed, we conclude that the number (one or two) of marginal cirri should be given as much consideration as the genetic separation. Given this, we reassessed the validity of using genetic separation to classify the group. Generally, the morphogenetic data disagreed with the molecular data (SSrRNA gene sequences). Given these discrepancies, it is too early to draw conclusions on the systematic arrangement of this species-rich taxon. 展开更多
关键词 EUPLOTES CILIATE ONTOGENESIS phylogeny sequences
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