目的:探讨阻断大鼠门静脉对肠屏障功能的影响,为临床门静脉阻断后肠屏障功能的保护提供实验依据。方法:SD大鼠70只,随机分为对照组、手术组。手术组门静脉阻断时间分别为30、40、60 m in,门静脉复流4 h。70只动物均于手术后5 h处死,无...目的:探讨阻断大鼠门静脉对肠屏障功能的影响,为临床门静脉阻断后肠屏障功能的保护提供实验依据。方法:SD大鼠70只,随机分为对照组、手术组。手术组门静脉阻断时间分别为30、40、60 m in,门静脉复流4 h。70只动物均于手术后5 h处死,无菌条件下取门静脉血2 m l检测内毒素;取回盲部肠系膜淋巴结作细菌培养;另在距回盲部2 cm处取小肠肠壁组织1 cm,作病理学检查。结果:对照组与手术组比较,大鼠门静脉阻断后其门静脉血清内毒素水平明显上升;肠系膜淋巴结细菌培养阳性率增加;小肠壁形态学结构亦发生明显改变。上述改变随门静脉阻断时间的延长而更加明显。结论:大鼠门静脉阻断后可导致其肠屏障功能的损害,其损害程度与门静脉阻断时间呈正相关。展开更多
目的观察不同部位及性质胆石症患者肠黏膜上皮细胞增殖及凋亡的改变，并探讨肠屏障功能在胆石形成中的作用和意义。方法按结石部位不同将108例胆石症患者分为三组：胆囊结石组（B1组，n＝36），胆管结石组（C1组，n ＝36），胆囊结石合...目的观察不同部位及性质胆石症患者肠黏膜上皮细胞增殖及凋亡的改变，并探讨肠屏障功能在胆石形成中的作用和意义。方法按结石部位不同将108例胆石症患者分为三组：胆囊结石组（B1组，n＝36），胆管结石组（C1组，n ＝36），胆囊结石合并胆管结石组（D1组，n ＝36），另设健康对照组（A1组，n ＝20）；根据结石不同性质分为胆固醇结石（B2组，n＝40），胆色素结石（C2组，n＝52），混合性结石组（D2组，n＝16），另设健康对照组（A2组，n＝20）；分别检测回肠末段黏膜组织学改变及肠黏膜上皮细胞增殖与凋亡率，并对检测结果进行对比分析。结果①不同部位结石患者A1、B1、C1、D1四组之间肠黏膜损伤程度、细胞凋亡与增殖率相比较均无显著性差异（ P ＞0．05）；②不同性质结石患者之间肠黏膜损伤程度、细胞凋亡与增殖率比较，C2组与B2组及A2组比较有统计学意义（均 P ＜0．05），与D2组比较，差异没有统计学意义（ P ＞0．05）。结论：胆色素结石患者均存在肠黏膜肠黏膜形态的损伤、细胞增殖下降和细胞凋亡增加的改变，胆色素结石形成与肠屏障功能损伤一定的相关性。肠黏膜屏障功能损伤可能在促进胆色素结石的形成中发挥一定的作用。展开更多
[Objective] The aim of this study was to investigate effects of rhubarb decoction with different concentrations on the isolated intestinal movement performance of Ophicephalus argus,and to discuss its pharmacological ...[Objective] The aim of this study was to investigate effects of rhubarb decoction with different concentrations on the isolated intestinal movement performance of Ophicephalus argus,and to discuss its pharmacological mechanism.[Method] With RM6240C biological signal collection and processing system,tension values of the isolated intestine before and after the treatment by rhubarb decoction with different concentrations were recorded by the isolated organ method.[Result] Rhubarb decoction with concentration of 0.062 and 0.125 g/ml showed obvious enhancement on the isolated intestinal contraction of Ophicephalus argus,while rhubarb decoction with concentration of 0.250 and 0.500 g/ml showed inhibition on the isolated intestinal contraction of Ophicephalus argus.There was an extremely significant difference in rhubarb decoction with concentration of lower than 0.500 g/ml and more than 0.500 g/ml.With the increase of rhubarb concentration,the intestinal movement of Ophicephalus argus enhanced,but the contraction frequency did not change obviously.[Conclusion] Rhubarb decoction has remarkable effects on the contraction of smooth muscle in the isolated intestines of Ophicephalus argus.展开更多
文摘目的:探讨阻断大鼠门静脉对肠屏障功能的影响,为临床门静脉阻断后肠屏障功能的保护提供实验依据。方法:SD大鼠70只,随机分为对照组、手术组。手术组门静脉阻断时间分别为30、40、60 m in,门静脉复流4 h。70只动物均于手术后5 h处死,无菌条件下取门静脉血2 m l检测内毒素;取回盲部肠系膜淋巴结作细菌培养;另在距回盲部2 cm处取小肠肠壁组织1 cm,作病理学检查。结果:对照组与手术组比较,大鼠门静脉阻断后其门静脉血清内毒素水平明显上升;肠系膜淋巴结细菌培养阳性率增加;小肠壁形态学结构亦发生明显改变。上述改变随门静脉阻断时间的延长而更加明显。结论:大鼠门静脉阻断后可导致其肠屏障功能的损害,其损害程度与门静脉阻断时间呈正相关。
文摘目的观察不同部位及性质胆石症患者肠黏膜上皮细胞增殖及凋亡的改变，并探讨肠屏障功能在胆石形成中的作用和意义。方法按结石部位不同将108例胆石症患者分为三组：胆囊结石组（B1组，n＝36），胆管结石组（C1组，n ＝36），胆囊结石合并胆管结石组（D1组，n ＝36），另设健康对照组（A1组，n ＝20）；根据结石不同性质分为胆固醇结石（B2组，n＝40），胆色素结石（C2组，n＝52），混合性结石组（D2组，n＝16），另设健康对照组（A2组，n＝20）；分别检测回肠末段黏膜组织学改变及肠黏膜上皮细胞增殖与凋亡率，并对检测结果进行对比分析。结果①不同部位结石患者A1、B1、C1、D1四组之间肠黏膜损伤程度、细胞凋亡与增殖率相比较均无显著性差异（ P ＞0．05）；②不同性质结石患者之间肠黏膜损伤程度、细胞凋亡与增殖率比较，C2组与B2组及A2组比较有统计学意义（均 P ＜0．05），与D2组比较，差异没有统计学意义（ P ＞0．05）。结论：胆色素结石患者均存在肠黏膜肠黏膜形态的损伤、细胞增殖下降和细胞凋亡增加的改变，胆色素结石形成与肠屏障功能损伤一定的相关性。肠黏膜屏障功能损伤可能在促进胆色素结石的形成中发挥一定的作用。
基金Supported by Teaching Reform Project of Tianjin Agricultural University (C-08-01)Scientific and Technological Project of Tianjin Sci-ence and Technology Commission (06YFGZNC02400)~~
文摘[Objective] The aim of this study was to investigate effects of rhubarb decoction with different concentrations on the isolated intestinal movement performance of Ophicephalus argus,and to discuss its pharmacological mechanism.[Method] With RM6240C biological signal collection and processing system,tension values of the isolated intestine before and after the treatment by rhubarb decoction with different concentrations were recorded by the isolated organ method.[Result] Rhubarb decoction with concentration of 0.062 and 0.125 g/ml showed obvious enhancement on the isolated intestinal contraction of Ophicephalus argus,while rhubarb decoction with concentration of 0.250 and 0.500 g/ml showed inhibition on the isolated intestinal contraction of Ophicephalus argus.There was an extremely significant difference in rhubarb decoction with concentration of lower than 0.500 g/ml and more than 0.500 g/ml.With the increase of rhubarb concentration,the intestinal movement of Ophicephalus argus enhanced,but the contraction frequency did not change obviously.[Conclusion] Rhubarb decoction has remarkable effects on the contraction of smooth muscle in the isolated intestines of Ophicephalus argus.
