复方氨基酸对短肠综合征大鼠肠粘膜形态结构的影响

Objective To investigate the effect of a new 20-crystalline aminoacids solution enriched with glutamine dipeptede and branch-chained animo acids(BCAA)on the morphological changes of the intestinal mucosa of SBS rats. Methods 32 male wistars rats were randomly allocated into 3 groups:12 rats in Gln-group,12 rats in Std-group,8 rats in Nitrogen-free group.Using the model of SBS rats,nutritional liquid with different aminoacids was given by central catheterization.The body weight,nitrogen balance,the total DNA and RNA of jejunalmucosal cells,jejunal mucosal thickness and villus height were measured. Results The results showed that all variable in 20AA group and 17AA group were better than no nitrogen(the second)group(P<0.05).The 20AA group had better body weight,nitrogen balance and jejunum morphology than 17AA group.Jejunal mucosalthickness and villus height,total DNA and RNA of jejunal mucosal cells in 20AA group was increased compared with 17AA group(P<0.05). Conclusions The result showed that 20AA TPN group had better nutritional support,passive nitrogen balance can be improved.It appears that 20AA TPN could be provided to maintain and support growth of the gestrointestinal mucose.

异麦芽低聚糖对断奶仔猪肠道VFA浓度、pH值及粘膜形态结构的影响

选择28日龄断奶仔猪54头 ,随机分为9组 ,分别饲喂3种不同日粮处理 ,每种日粮处理组为3个重复。3种日粮分别为添加0.05 %异麦芽低聚糖组(IMO组)、添加110mg/kg阿散酸组(抗生素组)和对照组。各组基础日粮相同。分别于35和42日龄从各重复中选1头屠宰 ,测定盲肠、结肠内容物中挥发性脂肪酸(VFA)浓度、pH值及盲肠、结肠粘膜上皮绒毛高度和隐窝深度。结果表明 ,35日龄时 ,IMO组盲肠乙酸、丙酸 ,结肠乙酸、丙酸及总VFA浓度均显著高于对照组(P<0.05) ;42日龄时 ,IMO组盲肠乙酸、丁酸及总VFA浓度 ,结肠乙酸浓度均显著或极显著(P<0.01)高于对照组 ;结肠绒毛高度受处理影响显著 ,在两次测定中 ,IMO组结肠绒毛高度均显著大于(P<0.05)对照组。35日龄时 ,抗生素组结肠绒毛高度也显著高于对照组(P<0.05)。