目的探讨2型糖尿病(T2DM)患者肠道菌群变化的临床意义。方法选取8例T2DM患者与10例体检健康者,提取其粪便基因组DNA,应用菌属16S rRNA V4序列特异性引物,采用高通量测序技术分析肠道菌群多样性(以香农多样性指数表示)及种类(以丰度表示...目的探讨2型糖尿病(T2DM)患者肠道菌群变化的临床意义。方法选取8例T2DM患者与10例体检健康者,提取其粪便基因组DNA,应用菌属16S rRNA V4序列特异性引物,采用高通量测序技术分析肠道菌群多样性(以香农多样性指数表示)及种类(以丰度表示)。观察不同性别T2DM患者的肠道菌群种类差异,分析T2DM患者肠道优势菌属与空腹血糖(FPG)、餐后1 h血糖(1 h PG)和餐后2 h血糖(2 h PG)的关系。结果 T2DM患者与体检健康者香农多样性指数分别为3.70±0.29、3.30±0.20,二者比较P>0.05。门水平下T2DM患者和体检健康者的肠道共同优势菌门为厚壁菌门、拟杆菌门、变形菌门和放线菌门,二者上述4种优势菌门丰度比较差异均无统计学意义(P均>0.05)。属水平下T2DM患者和体检健康者的肠道共同优势菌属为拟杆菌属和毛螺杆菌科,体检健康者罗斯氏菌属丰度高于T2DM患者(P<0.05)。女性T2DM患者拟杆菌属科丰度高于男性患者,假单胞菌属丰度低于男性患者(P均<0.05)。T2DM患者柔嫩梭菌属丰度与2 h PG呈正相关(r=0.75,P<0.05),其他肠道优势菌属与FPG、1 h PG、2 h PG均无关(P均>0.05)。结论 T2DM患者肠道内存在菌群失调,并具有性别差异;柔嫩梭菌属与血糖水平有关,可为T2DM的治疗提供一种新的思路。展开更多
Oral exposure to toxic metals such as cadmium （Cd）, lead （Pb）, copper （Cu） and aluminum （AI） can induce various adverse health effects in humans and animals. However, the effects of these metals on the gut mic...Oral exposure to toxic metals such as cadmium （Cd）, lead （Pb）, copper （Cu） and aluminum （AI） can induce various adverse health effects in humans and animals. However, the effects of these metals on the gut microbiota have received limited attention. The present study demonstrated that long-term toxic metal exposure altered the intestinal microbiota of mice in a metal-specific and time-dependent manner. Subchronic oral Cu exposure for eight weeks caused a profound decline in gut microbial diversity in mice, whereas no significant changes were observed in groups treated with other metals. Cd exposure signif- icantly increased the relative abundances of organisms from the genera Alistipes and Odoribacter and caused marked decreases in Mollicutes and unclassified Ruminococcaceae. Pb exposure significantly decreased the abundances of eight genera： unclassified and uncultured Ruminococcaceae, unclassified Lachnospiraceae, Ruminiclostridium_9, Rikenellaceae_RCg_gut group, Oscillibacter, Anaerotruncus and Lachnoclostridium. Cu exposure affected abundances of the genera Alistipes, Bacteroides, Ruminococcaceae_UCG-014, AUobaculum, Mollicutes_RF9_norank, Rikenellaceae_RCg gut_group, Ruminococcaceae_unclassified and Turicibacter. A1 exposure increased the abundance of Odoribacter and decreased that of Anaerotruncus. Exposure to any metal for eight weeks significantly decreased the abun- dance of Akkermansia. These results provide a new understanding regarding the role of toxic metals in the pathogenesis of intestinal and systemic disorders in the host within the gut microbiota framework.展开更多
文摘目的探讨2型糖尿病(T2DM)患者肠道菌群变化的临床意义。方法选取8例T2DM患者与10例体检健康者,提取其粪便基因组DNA,应用菌属16S rRNA V4序列特异性引物,采用高通量测序技术分析肠道菌群多样性(以香农多样性指数表示)及种类(以丰度表示)。观察不同性别T2DM患者的肠道菌群种类差异,分析T2DM患者肠道优势菌属与空腹血糖(FPG)、餐后1 h血糖(1 h PG)和餐后2 h血糖(2 h PG)的关系。结果 T2DM患者与体检健康者香农多样性指数分别为3.70±0.29、3.30±0.20,二者比较P>0.05。门水平下T2DM患者和体检健康者的肠道共同优势菌门为厚壁菌门、拟杆菌门、变形菌门和放线菌门,二者上述4种优势菌门丰度比较差异均无统计学意义(P均>0.05)。属水平下T2DM患者和体检健康者的肠道共同优势菌属为拟杆菌属和毛螺杆菌科,体检健康者罗斯氏菌属丰度高于T2DM患者(P<0.05)。女性T2DM患者拟杆菌属科丰度高于男性患者,假单胞菌属丰度低于男性患者(P均<0.05)。T2DM患者柔嫩梭菌属丰度与2 h PG呈正相关(r=0.75,P<0.05),其他肠道优势菌属与FPG、1 h PG、2 h PG均无关(P均>0.05)。结论 T2DM患者肠道内存在菌群失调,并具有性别差异;柔嫩梭菌属与血糖水平有关,可为T2DM的治疗提供一种新的思路。
基金supported by the National Natural Science Foundation of China Key Program(31530056)National Natural Science Foundation of China(31601452)+3 种基金the General Financial Grant from the China Postdoctoral Science Foundation(2016M590412)the Natural Science Foundation of Jiangsu Province(BK20160175)the General Financial Grant from the Jiangsu Postdoctoral Science Foundation(1601113C)BBSRC Newton Fund Joint Centre Award
文摘Oral exposure to toxic metals such as cadmium （Cd）, lead （Pb）, copper （Cu） and aluminum （AI） can induce various adverse health effects in humans and animals. However, the effects of these metals on the gut microbiota have received limited attention. The present study demonstrated that long-term toxic metal exposure altered the intestinal microbiota of mice in a metal-specific and time-dependent manner. Subchronic oral Cu exposure for eight weeks caused a profound decline in gut microbial diversity in mice, whereas no significant changes were observed in groups treated with other metals. Cd exposure signif- icantly increased the relative abundances of organisms from the genera Alistipes and Odoribacter and caused marked decreases in Mollicutes and unclassified Ruminococcaceae. Pb exposure significantly decreased the abundances of eight genera： unclassified and uncultured Ruminococcaceae, unclassified Lachnospiraceae, Ruminiclostridium_9, Rikenellaceae_RCg_gut group, Oscillibacter, Anaerotruncus and Lachnoclostridium. Cu exposure affected abundances of the genera Alistipes, Bacteroides, Ruminococcaceae_UCG-014, AUobaculum, Mollicutes_RF9_norank, Rikenellaceae_RCg gut_group, Ruminococcaceae_unclassified and Turicibacter. A1 exposure increased the abundance of Odoribacter and decreased that of Anaerotruncus. Exposure to any metal for eight weeks significantly decreased the abun- dance of Akkermansia. These results provide a new understanding regarding the role of toxic metals in the pathogenesis of intestinal and systemic disorders in the host within the gut microbiota framework.
