研究旨在观察包被精油复合酸(microencapsulated complex of essential oils and organic acids,MEA)对断奶仔猪生长性能、免疫相关指标和盲肠微生物的影响及其机理探讨。选用断奶仔猪250头,随机分为5组,每组设5个重复,每个重复10头猪,...研究旨在观察包被精油复合酸(microencapsulated complex of essential oils and organic acids,MEA)对断奶仔猪生长性能、免疫相关指标和盲肠微生物的影响及其机理探讨。选用断奶仔猪250头,随机分为5组,每组设5个重复,每个重复10头猪,分别饲喂基础日粮(BASE组)、基础日粮+0.05%MEA2(LA组)、基础日粮+0.08%MEA2(MA组)、基础日粮+0.20%MEA1(HA组)、基础日粮+0.20%MEA1+0.05%MEA2(BHA组),饲养试验时间为21d。结果表明,LA、MA组断奶仔猪肝脏指数显著低于BASE组(P<0.05),但在日粮中添加MEA对断奶仔猪生长性能、血清IL-2、回肠Occludin mRNA相对表达量与空肠SlgA无显著影响(P>0.05);BASE、LA组、MA组回肠pH值均显著高于BHA组(P<0.05),且随着MEA添加量的增加,回肠pH值随之降低(P<0.01);MA组较LA组或HA组相比有增加血清IgG的趋势(0.05<P<0.10);MA组较BASE组或BHA组显著增加了回肠ZO-1基因相对表达量,MA组较BASE组有增加回肠IL-10基因相对表达量的趋势(0.05<P<0.10);MA组盲肠双歧杆菌数量有高于BASE组的趋势(0.05<P<0.10),MA组盲肠双歧杆菌显著高于LA组(P<0.05),LA组与BASE组差异不显著(P>0.05)。结果提示,在日粮中添加MEA可以降低断奶仔猪回肠pH值;在日粮中添加MEA2可以显著降低断奶仔猪肝脏指数;在日粮中添加0.08%的MEA2可以增强断奶仔猪免疫强度并可改善断奶仔猪盲肠微生物菌群结构。展开更多
Tea is one of the most popular beverages, consumed by over two thirds of the world’s population; but the aluminum accumulation property of tea plant is becoming the focus of many researches because of aluminum’s kn...Tea is one of the most popular beverages, consumed by over two thirds of the world’s population; but the aluminum accumulation property of tea plant is becoming the focus of many researches because of aluminum’s known adverse effect on human health. Investigation of the interactions of catechins with Al3+ showed that during the interaction of catechins with Al3+, the UV-vis spectrum of catechins was changed. Absorption of EGCG at 274 nm decreased and increased at 322 nm; EC and C’s at 278 nm changed little. The ratio of Al3+ to EGCG was 1:1 in pH 5.0 buffer solution; in pH 6.2 buffer solution, the ratio in the Al-EGCG complex was 1:1. Interestingly, while the ratio reached to over 2, after the complex of Al-EGCG started polymerization, the ratio in the polymer was 2:1. In pH 6.2 buffer solution, the complex behavior of C with Al3+ was the same as that of EGCG, with a little difference for EC. When the ratio of Al3+ to EC was <1, the complex in ratio was 1:2, but, the complex polymerized when the ratio of Al3+ to EC was >1. It was found that the ratio of Al3+ to EC in the polymer was 1:1. Polymerization of Al-catechin complexes might reduce aluminum absorption in the intestine. Kow value was also employed to study the properties of aluminum species in tea infusion (at gastric and intestine pH condition) and the effect of catechins and tea polyphenols on Kow in buffer solution. Results showed that Kow value rose much higher at the intestine pH than at the gastric pH. Tea polyphenols and catechins could greatly reduce aluminum Kow value in acetic buffer, indicating that these compounds may reduce aluminum absorption during tea intake.展开更多
文摘研究旨在观察包被精油复合酸(microencapsulated complex of essential oils and organic acids,MEA)对断奶仔猪生长性能、免疫相关指标和盲肠微生物的影响及其机理探讨。选用断奶仔猪250头,随机分为5组,每组设5个重复,每个重复10头猪,分别饲喂基础日粮(BASE组)、基础日粮+0.05%MEA2(LA组)、基础日粮+0.08%MEA2(MA组)、基础日粮+0.20%MEA1(HA组)、基础日粮+0.20%MEA1+0.05%MEA2(BHA组),饲养试验时间为21d。结果表明,LA、MA组断奶仔猪肝脏指数显著低于BASE组(P<0.05),但在日粮中添加MEA对断奶仔猪生长性能、血清IL-2、回肠Occludin mRNA相对表达量与空肠SlgA无显著影响(P>0.05);BASE、LA组、MA组回肠pH值均显著高于BHA组(P<0.05),且随着MEA添加量的增加,回肠pH值随之降低(P<0.01);MA组较LA组或HA组相比有增加血清IgG的趋势(0.05<P<0.10);MA组较BASE组或BHA组显著增加了回肠ZO-1基因相对表达量,MA组较BASE组有增加回肠IL-10基因相对表达量的趋势(0.05<P<0.10);MA组盲肠双歧杆菌数量有高于BASE组的趋势(0.05<P<0.10),MA组盲肠双歧杆菌显著高于LA组(P<0.05),LA组与BASE组差异不显著(P>0.05)。结果提示,在日粮中添加MEA可以降低断奶仔猪回肠pH值;在日粮中添加MEA2可以显著降低断奶仔猪肝脏指数;在日粮中添加0.08%的MEA2可以增强断奶仔猪免疫强度并可改善断奶仔猪盲肠微生物菌群结构。
文摘Tea is one of the most popular beverages, consumed by over two thirds of the world’s population; but the aluminum accumulation property of tea plant is becoming the focus of many researches because of aluminum’s known adverse effect on human health. Investigation of the interactions of catechins with Al3+ showed that during the interaction of catechins with Al3+, the UV-vis spectrum of catechins was changed. Absorption of EGCG at 274 nm decreased and increased at 322 nm; EC and C’s at 278 nm changed little. The ratio of Al3+ to EGCG was 1:1 in pH 5.0 buffer solution; in pH 6.2 buffer solution, the ratio in the Al-EGCG complex was 1:1. Interestingly, while the ratio reached to over 2, after the complex of Al-EGCG started polymerization, the ratio in the polymer was 2:1. In pH 6.2 buffer solution, the complex behavior of C with Al3+ was the same as that of EGCG, with a little difference for EC. When the ratio of Al3+ to EC was <1, the complex in ratio was 1:2, but, the complex polymerized when the ratio of Al3+ to EC was >1. It was found that the ratio of Al3+ to EC in the polymer was 1:1. Polymerization of Al-catechin complexes might reduce aluminum absorption in the intestine. Kow value was also employed to study the properties of aluminum species in tea infusion (at gastric and intestine pH condition) and the effect of catechins and tea polyphenols on Kow in buffer solution. Results showed that Kow value rose much higher at the intestine pH than at the gastric pH. Tea polyphenols and catechins could greatly reduce aluminum Kow value in acetic buffer, indicating that these compounds may reduce aluminum absorption during tea intake.
