目的:观察健脾渗湿、调和气血中药内外同治对轻-中度活动期溃疡性结肠炎的临床疗效。方法:采用随机对照试验研究,将符合纳入标准的轻-中度活动期溃疡性结肠炎患者37例随机分为治疗组20例,对照组17例。其中治疗组:健脾渗湿、调和气血中...目的:观察健脾渗湿、调和气血中药内外同治对轻-中度活动期溃疡性结肠炎的临床疗效。方法:采用随机对照试验研究,将符合纳入标准的轻-中度活动期溃疡性结肠炎患者37例随机分为治疗组20例,对照组17例。其中治疗组:健脾渗湿、调和气血中药内外同治:中药口服(100 m L,2次/d)联合中药气流导入弥散灌肠(200 m L,1次/d);对照组:莎尔福(美沙拉嗪肠溶片),1.0 g,口服,3次/d,分别记录并比较两组患者的临床症状、Southerland疾病活动指数(DAI)以及结肠镜检查Baron's积分变化情况。结果:完成本研究者32例(治疗组17例,对照组15例),两组患者总有效率分别为82.35%和66.67%(P<0.05),治疗2个月后两组患者在腹泻腹痛及黏液脓血便症状积分均有明显降低,且治疗组优于对照组(P<0.05),成功缓解时间明显提前;两组患者的Southerland DAI及Baron's积分均有显著改善(P<0.05),但组间差异无统计学意义(P>0.05)。结论:健脾渗湿、调和气血中药内外同治方案能够有效缓解轻-中度活动期溃疡性结肠炎相关症状及体征,为临床诊治本病提供新的视角。展开更多
In this study,circular dichroism(CD)and molecular dynamics(MD)simulation were used to investigate the thermal unfolding pathway of staphylococcal enterotoxin B(SEB)at temperatures of 298–371 and 298–500 K,and the re...In this study,circular dichroism(CD)and molecular dynamics(MD)simulation were used to investigate the thermal unfolding pathway of staphylococcal enterotoxin B(SEB)at temperatures of 298–371 and 298–500 K,and the relationship between the experimental and simulation results were explored.Our computational findings on the secondary structure of SEB showed that at room temperature,the CD spectroscopic results were highly consistent with the MD results.Moreover,under heating conditions,the changing trends of helix,sheet and random coil obtained by CD spectral fitting were highly consistent with those obtained by MD.In order to gain a deeper understanding of the thermal stability mechanism of SEB,the MD trajectories were analyzed in terms of root mean square deviation(RMSD),secondary structure assignment(SSA),radius of gyration(R_(g)),free energy surfaces(FES),solvent-accessible surface area(SASA),hydrogen bonds and salt bridges.The results showed that at low heating temperature,domain Ⅰ without loops(omitting the mobile loop region)mainly relied on hydrophobic interaction to maintain its thermal stability,whereas the thermal stability of domain Ⅱ was mainly controlled by salt bridges and hydrogen bonds.Under high heating temperature conditions,the hydrophobic interactions in domain Ⅰ without loops were destroyed and the secondary structure was almost completely lost,while domain Ⅱ could still rely on salt bridges as molecular staples to barely maintain the stability of the secondary structure.These results help us to understand the thermodynamic and kinetic mechanisms that maintain the thermal stability of SEB at the molecular level,and provide a direction for establishing safer and more effective food sterilization processes.展开更多
文摘目的:观察健脾渗湿、调和气血中药内外同治对轻-中度活动期溃疡性结肠炎的临床疗效。方法:采用随机对照试验研究,将符合纳入标准的轻-中度活动期溃疡性结肠炎患者37例随机分为治疗组20例,对照组17例。其中治疗组:健脾渗湿、调和气血中药内外同治:中药口服(100 m L,2次/d)联合中药气流导入弥散灌肠(200 m L,1次/d);对照组:莎尔福(美沙拉嗪肠溶片),1.0 g,口服,3次/d,分别记录并比较两组患者的临床症状、Southerland疾病活动指数(DAI)以及结肠镜检查Baron's积分变化情况。结果:完成本研究者32例(治疗组17例,对照组15例),两组患者总有效率分别为82.35%和66.67%(P<0.05),治疗2个月后两组患者在腹泻腹痛及黏液脓血便症状积分均有明显降低,且治疗组优于对照组(P<0.05),成功缓解时间明显提前;两组患者的Southerland DAI及Baron's积分均有显著改善(P<0.05),但组间差异无统计学意义(P>0.05)。结论:健脾渗湿、调和气血中药内外同治方案能够有效缓解轻-中度活动期溃疡性结肠炎相关症状及体征,为临床诊治本病提供新的视角。
文摘In this study,circular dichroism(CD)and molecular dynamics(MD)simulation were used to investigate the thermal unfolding pathway of staphylococcal enterotoxin B(SEB)at temperatures of 298–371 and 298–500 K,and the relationship between the experimental and simulation results were explored.Our computational findings on the secondary structure of SEB showed that at room temperature,the CD spectroscopic results were highly consistent with the MD results.Moreover,under heating conditions,the changing trends of helix,sheet and random coil obtained by CD spectral fitting were highly consistent with those obtained by MD.In order to gain a deeper understanding of the thermal stability mechanism of SEB,the MD trajectories were analyzed in terms of root mean square deviation(RMSD),secondary structure assignment(SSA),radius of gyration(R_(g)),free energy surfaces(FES),solvent-accessible surface area(SASA),hydrogen bonds and salt bridges.The results showed that at low heating temperature,domain Ⅰ without loops(omitting the mobile loop region)mainly relied on hydrophobic interaction to maintain its thermal stability,whereas the thermal stability of domain Ⅱ was mainly controlled by salt bridges and hydrogen bonds.Under high heating temperature conditions,the hydrophobic interactions in domain Ⅰ without loops were destroyed and the secondary structure was almost completely lost,while domain Ⅱ could still rely on salt bridges as molecular staples to barely maintain the stability of the secondary structure.These results help us to understand the thermodynamic and kinetic mechanisms that maintain the thermal stability of SEB at the molecular level,and provide a direction for establishing safer and more effective food sterilization processes.
