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坏死股骨头、股骨粗隆间及正常骨组织中VEGF表达比较 被引量:5
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作者 阚金庆 司端涛 +1 位作者 高忠礼 杨玉宝 《山东医药》 CAS 北大核心 2016年第16期62-63,共2页
目的比较血管内皮生长因子(VEGF)在坏死股骨头、股骨粗隆间及正常骨组织中的表达变化,为股骨头缺血性坏死(ONFH)发病机制的研究提供依据。方法选择需行人工关节置换术的股骨粗隆间骨折患者10例,术中取其正常股骨头组织和股骨粗隆间骨组... 目的比较血管内皮生长因子(VEGF)在坏死股骨头、股骨粗隆间及正常骨组织中的表达变化,为股骨头缺血性坏死(ONFH)发病机制的研究提供依据。方法选择需行人工关节置换术的股骨粗隆间骨折患者10例,术中取其正常股骨头组织和股骨粗隆间骨组织;需行人工关节置换术的ONFH患者10例,术中取其坏死股骨头组织。采用Real-time PCR法检测各组织中VEGF mRNA相对表达量。结果正常股骨头组织、股骨粗隆间骨组织及坏死股骨头组织中VEGF mRNA相对表达量分别为0.55±0.28、0.51±0.38、0.43±0.43,正常股骨头组织及粗隆间骨组织VEGF mRNA相对表达量均明显高于坏死股骨头组织(P均<0.05),正常股骨头组织与粗隆间骨组织VEGF mRNA相对表达量比较无统计学差异(P>0.05)。结论坏死股骨头组织VEGF表达低于股骨粗隆间及正常骨组织,骨组织VEGF表达降低可能与ONFH的发病有关。 展开更多
关键词 血管内皮生长因子 股骨头缺血坏死性 聚合酶链反应 骨组织
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股骨头坏死患者坏死骨组织中miRNA表达谱变化 被引量:2
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作者 杨玉宝 刘洪亚 +2 位作者 阚金庆 高忠礼 李林 《山东医药》 CAS 北大核心 2015年第34期74-75,共2页
目的观察股骨头坏死患者坏死骨组织中miRNA表达谱变化,探讨miRNA在股骨头坏死发生发展中的作用。方法选择正常股骨粗隆间骨折(对照组)、股骨头坏死(观察组)患者各4例,分别取正常及坏死股骨头骨组织,采用基因芯片技术检测miRNA表达谱变化... 目的观察股骨头坏死患者坏死骨组织中miRNA表达谱变化,探讨miRNA在股骨头坏死发生发展中的作用。方法选择正常股骨粗隆间骨折(对照组)、股骨头坏死(观察组)患者各4例,分别取正常及坏死股骨头骨组织,采用基因芯片技术检测miRNA表达谱变化;经聚类分析寻找明显上调和下调的基因,以RT-PCR法检测其表达量。结果两组采用miRNA芯片检测426个miRNA,上调比例≥2的有2条(hsa-miR-494-st和hsa-miR-29a-st),下调比例≤0.5的有1条(hsa-miR-486-5p-st)。观察组hsa-miR-29a-st的相对表达量为3.56±5.02,对照组为1.16±0.15,两组比较,P<0.05;观察组hsa-miR-494-st、hsa-miR-486-5p-st的相对表达量分别为2.32±1.42、1.85±1.24,对照组分别为4.13±3.18、3.78±2.69,两组比较,P均>0.05。结论股骨头坏死患者坏死骨组织中存在miRNA表达谱变化,其中hsa-miR-29a-st可能在股骨头坏死发生、发展过程中起调控作用。 展开更多
关键词 缺血坏死性股骨头 microRNA 聚合酶链反应 基因芯片
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IGF-1在正常与坏死股骨头骨组织中的表达差异 被引量:1
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作者 赵鹏飞 李云萍 +4 位作者 李晓明 宋祥义 苏明红 左世锋 杨玉宝 《山东医学高等专科学校学报》 2017年第2期122-124,F0003,共4页
目的通过RT-PCR及荧光原位杂交方法观察胰岛素样生长因子-1(IGF-1)在正常股骨头与缺血坏死性股骨头组织中的表达差异,探讨IGF-1在股骨头坏死中的发病机制。方法取股骨粗隆间骨折及缺血坏死性股骨头行人工股骨头置换或全髋人工关节的正... 目的通过RT-PCR及荧光原位杂交方法观察胰岛素样生长因子-1(IGF-1)在正常股骨头与缺血坏死性股骨头组织中的表达差异,探讨IGF-1在股骨头坏死中的发病机制。方法取股骨粗隆间骨折及缺血坏死性股骨头行人工股骨头置换或全髋人工关节的正常股骨头、缺血坏死性股骨头骨组织各10例;分别取组织2~5g放入冷冻管存入液氮用于荧光原位杂交及IGF-1基因的RT-PCR。结果 IGF-1的荧光原位杂交图像显示正常骨组织中的表达量比坏死组织增多,RTPCR的2-ΔΔCt平均值分别为0.58±0.34;0.43±0.19;正常股骨头骨组织中IGF-1基因RT-PCR的2-ΔΔCt平均值与缺血坏死性股骨头骨组织平均值相比,差异有统计学意义(P<0.05)。结论IGF-1基因在正常与缺血坏死性股骨头骨组织表达不同,IGF基因表达量的减少可能是股骨头缺血坏死的重要原因之一。 展开更多
关键词 IGF-1 缺血坏死性股骨头 荧光原位杂交 聚合酶链反应
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BMP-2在正常与坏死股骨头骨组织中的表达差异
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作者 赵鹏飞 李云萍 +4 位作者 李晓明 宋祥义 苏明红 左世锋 杨玉宝 《中国医药指南》 2017年第10期31-32,共2页
目的通过荧光原位杂交及RT-PCR实验方法观察骨形态发生蛋白-2(BMP-2)在正常与缺血坏死性股骨头骨组织中的表达差异,探讨BMP-2在两种骨组织中的变化规律。方法取股骨粗隆间骨折及缺血坏死性股骨头需行人工股骨头置换或全髋人工关节置换... 目的通过荧光原位杂交及RT-PCR实验方法观察骨形态发生蛋白-2(BMP-2)在正常与缺血坏死性股骨头骨组织中的表达差异,探讨BMP-2在两种骨组织中的变化规律。方法取股骨粗隆间骨折及缺血坏死性股骨头需行人工股骨头置换或全髋人工关节置换的正常股骨头、缺血坏死性股骨头骨组织患者各8例;分别取组织2~5 g放入冷冻管存入液氮用于荧光原位杂交及BMP-2基因的RT-PCR。结果BMP-2的荧光原位杂交图像显示正常骨组织中的表达量比坏死组织增多,RT-PCR的2-ΔΔCt平均值分别为0.55±0.35,0.45±0.20;正常股骨头骨组织中BMP-2基因RT-PCR的2-ΔΔCt平均值与缺血坏死性股骨头骨组织平均值相比P<0.05。结论 BMP-2基因在正常与缺血坏死性股骨头骨组织有统计学意义,因此BMP-2基因表达量的减少也许是股骨头缺血坏死的重要原因之一。 展开更多
关键词 正常股骨头 缺血坏死性股骨头 荧光原位杂交 聚合酶链反应
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CGRP在坏死股骨头中的基因表达
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作者 宋祥义 苏明红 +4 位作者 左世锋 朱洪征 赵光辉 赵鹏飞 杨玉宝 《山东医学高等专科学校学报》 2017年第6期415-418,共4页
目的通过采用RT-PCR方法观察降钙素基因相关肽(CGRP)在正常股骨头组织与缺血坏死性股骨头组织中基因的表达差异,探讨CGRP在股骨头坏死中的发病机制。方法分别取正常股骨头、缺血坏死性股骨头骨组织各12例;分别取骨组织3~4g放入冷冻管存... 目的通过采用RT-PCR方法观察降钙素基因相关肽(CGRP)在正常股骨头组织与缺血坏死性股骨头组织中基因的表达差异,探讨CGRP在股骨头坏死中的发病机制。方法分别取正常股骨头、缺血坏死性股骨头骨组织各12例;分别取骨组织3~4g放入冷冻管存入液氮,用于CGRP基因的RT-PCR。结果 CGRP在正常骨组织中的表达量比其在坏死组织中明显减少,RTPCR的2^(-ΔΔCt)平均值分别为(0.42±0.24)和(0.53±0.35);正常股骨头骨组织中CGRP基因RTPCR的2^(-ΔΔCt)平均值与缺血坏死性股骨头骨组织平均值相比,P<0.05。结论 CGRP基因在正常与缺血坏死性股骨头骨组织中的表达量明显不同,因此CGRP基因表达量的增多也许是股骨头缺血坏死的重要原因之一。 展开更多
关键词 CGRP 缺血坏死性股骨头 RTPCR
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酒精性与正常股骨头骨组织中微小RNA基因的多态性分析 被引量:3
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作者 杨玉宝 刘省臣 +3 位作者 宋扬 阚金庆 高忠礼 李林 《中华实验外科杂志》 CAS CSCD 北大核心 2013年第4期799-801,共3页
目的观察微小RNA(miRNA)在酒精缺血坏死性与正常股骨头骨组织的表达变化,探讨miRNA基因在两者中的变化关系。方法分别对5例酒精性股骨头与5例正常股骨头骨组织进行miRNA基因芯片检测,可见有上调基因has.miR-29a.st和下调基因has.... 目的观察微小RNA(miRNA)在酒精缺血坏死性与正常股骨头骨组织的表达变化,探讨miRNA基因在两者中的变化关系。方法分别对5例酒精性股骨头与5例正常股骨头骨组织进行miRNA基因芯片检测,可见有上调基因has.miR-29a.st和下调基因has.miR-486-5p.st,分别对上调和下调基因行逆转录-聚合酶链反应(1iT—PCR)。结果miRNA基因芯片共检测酒精缺血坏死性与正常股骨头骨组织400条基因,对明显上调has—miR-29a—st和下调has.miR-486-5p.st基因片段行RT.PCR检测,正常股骨头和酒精坏死性股骨头上调基因的2-△△ct平均值为1.06±0.09和1.93±1.12,下调基因的2-△△ct平均值为1.88±25.37和1.70±0.98(变化〉2倍且P〈0.05)。结论酒精缺血坏死性与正常股骨头骨组织存在明显miRNA变化,其可能在股骨头坏死发生过程中可能起着重要的调控作用。 展开更多
关键词 酒精缺血坏死性股骨头 基因芯片
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临床推拿医案六则
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作者 聂怀利 《按摩与导引》 2009年第8期54-55,共2页
临床诊疗过程中,或有症状与病因不符者,或有诊断不明或治疗不当者,而留下许多经验和教训。现将在临症中所遇六个典型病例介绍给大家。
关键词 肩疼 肺癌性 双肩痛 糖尿病性 腰痛 肺癌性 腰痛 骨癌性腰 腿痛 股骨头坏死性 案例
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Changes of bone mineral in steroid-induced osteonecrosis in rabbits
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作者 WANG Tao WANG Kunzheng +6 位作者 WANG Lei GUO Jian GAO Ying ZHANG Zengtie JIN Liaosha WANG Chunsheng WANG Wei 《Journal of Medical Colleges of PLA(China)》 CAS 2013年第2期80-91,共12页
To investigate the significance of bone mineral changes in the pathogenic process of steroid-induced osteonecrosis in rabbits. Methods: Seventy adult female Japanese White rabbits were randomly divided into five grou... To investigate the significance of bone mineral changes in the pathogenic process of steroid-induced osteonecrosis in rabbits. Methods: Seventy adult female Japanese White rabbits were randomly divided into five groups Animals in steroid group received an intramuscular injection of methylprednisolone acetate (4 mg/kg) once a week, and killed in four different time after first treatment. Animals in control group received an intramuscular injection with the same volume of normal saline, while 150 kU penicillin was injected twice a week for preventing infection in all groups. The contents of serum calcium (Ca) and phosphorus (P) were determined and the bone mineral densities of the femoral head and neck were measured at the 2nd, 4th, 8th and 10th weeks after first treatment. Meanwhile the general condition such as weight, spirit and hair of rabbits was observed. Results: Compared with the control group, the weight of rabbits in steroid group lost since the first week, and progressively aggravated; the bone mineral density of the femoral head and neck was lowered since the 2nd and 4th week respectively (P〈0.05, P〈0.01), and lowered more quickly in femoral neck than in femoral head. In steroid group, the bone mineral density of the femoral neck at the 10th week was significantly lower than that at the 8th week (P〈0.05) Compared with the control group, the serum levels of Ca and P in steroid group were lowered since the 2nd week (P〈0.05, P〈0.01). In steroid group, the serum Ca level at the 10th week was lower than that at the 8th week, and the serum P level waslowered more quickly than Ca level was. Conclusion: Osteonecrosis appears in rabbits shortly after corticoids are first administered, and osteonecrosis in rabbits is chronologically associated with the onset of hypocalcaemia, hypophosphatemia and lowered bone mineral density. Steroid-induced osteonecrosis may be relevant to bone mineral loss and density lowering of the femoral head and neck. 展开更多
关键词 Femur head necrosis GLUCOCORTICOIDS Bone density CALCIUM PHOSPHORUS
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Osteoinductive activity of demineralized bone matrix and deprotenized bone derived from human avascular necrotic femoral head 被引量:3
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作者 张波 刘明勇 赵建华 《Chinese Journal of Traumatology》 CAS 2009年第6期379-383,共5页
Objective: To observe the osteoinductive activity of demineralized bone matrix (DBM) and deprotenized bone (DPB) made from human avascular necrotic femoral head. Methods: The femoral head was cut into pieces wi... Objective: To observe the osteoinductive activity of demineralized bone matrix (DBM) and deprotenized bone (DPB) made from human avascular necrotic femoral head. Methods: The femoral head was cut into pieces with the size of 3 mm×3 mm×5 mm, which were made into DBM and DPB. These two kinds of biomaterials were cocultured with human bone mesenchymal stem cells (hBMSCs). Monolayer cells without biomaterials were cultured as control. Proliferative activity ofhBMSCs was evaluated on days 1, 3, 5, 7 and 14. The concentration of alkaline phosphatase (ALP), osteocalcin (OC), and Ca^2+ were detected on days 1, 7, 14 and 21. Results: Cells cultured in DBM showed higher proliferative activity than did in DPB and monolayer cells (F= 39.773, P〈0.01). DBM and DPB also had osteoinductive activity. The concentrations of ALP (F=93.162, P〈0.01), OC (F=236.852, P〈0.01), Ca^2+ (F=80.711, P〈0.01)of DBM group were significantly higher than that of DPB and control groups. Conclusions: In vitro, DBM and DPB made from avascular necrotic femoral head have osteoinductive activity when cocultured with hBMSCs, and the former is stronger than the latter. 展开更多
关键词 Femur head Bone matrix Mesenchymal stem cells Bone substitutes
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Prediction of traumatic avascular necrosis of the femoral head by single photon emission computerized tomography and computerized tomography: an experimental study in dogs 被引量:6
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作者 SHEN Feng YAN Zuo-qin GUO Chang-an SHI Hong-cheng GU Yu-shen ZENG Meng-su LU Xiao-yu LIU Jun 《Chinese Journal of Traumatology》 CAS 2011年第4期227-232,共6页
Objective: To evaluate the femoral head perfusion and to predict the traumatic avascular necrosis (AVN) of the femoral head by single photon emission computerized tomography and computerized tomography (SPECT/CT)... Objective: To evaluate the femoral head perfusion and to predict the traumatic avascular necrosis (AVN) of the femoral head by single photon emission computerized tomography and computerized tomography (SPECT/CT). Methods: Totally 18 adult beagle dogs were divided randomly into three equal-sized (n=6) groups. Subsequently different degrees of ischemia model were developed by de- stroying blood vessels of the femoral head. The left hip received sham operation as normal control and the right hip underwent blood interruption. In Group A, the ligamentum teres was cut off. In Group B, the marrow cavity of the right femoral neck was destroyed while in Group C, the soft tis- sues at the base of the femoral neck were stripped in addition to the resection of the ligamentum teres and destruction of the marrow cavity. Three hours after surgery, SPECT/ CT was performed. Laser Doppler Flowmetry (LDF) measurements were also obtained at three different time points (before operation, immediately and three hours after operation) in order to assess the change process of blood supply to the femoral head. Results: SPECT/CT showed no significant difference in the radionuclide uptake between the right and left femo- ral heads in Group A (t=-0.09,P=-0.94) and Group B (t= 0.52, P=0.62). However, in Group C, it was 261±62 for the right femoral head, only 12% of that in the left femoral head. LDF measurements indicated that the femoral head perfusion was decreased from (45.0±3.3) PU to (39.1±3.7) PU in Group A, from (44.0±2.7) PU to (34.3±2.6) PU in Group B, and from (47.3±2.1) PU to (4.96±0.6) PU in Group C immediately after operation. However, the perfusion was restored and returned to normal values three hours after operation except in Group C. Conclusion: SPECT/CT could assess the perfusion of the femoral head semiquantitatively, which might be useful in predicting the development of traumatic AVN. 展开更多
关键词 Femur head necrosis Femoral head Tomography emission-computed single-photon Tomography X-ray computed Magnetic resonance imaging
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Ipsilateral fracture dislocations of the hip and knee joints with contralateral open fracture of the leg: a rare case and its management principles 被引量:5
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作者 Ramesh Kumar Sen Sujit Kumar Tripathy Vibhu Krishnan Tarun Goyal Vanyambadi Jagadeesh 《Chinese Journal of Traumatology》 CAS 2011年第3期183-187,共5页
This paper discussed the injury mechanism and management of a patient who had concomitant ipsilateral hip and knee dislocations and contralateral open leg fracture. A 32-year-old man presented with ipsilateral fractu... This paper discussed the injury mechanism and management of a patient who had concomitant ipsilateral hip and knee dislocations and contralateral open leg fracture. A 32-year-old man presented with ipsilateral fracturedislocations of the left hip (Pipkin's type IV) and knee (Moore II)joints and contralateral open fracture of the leg bones after a car accident. After emergency resuscitative measures, the hip joint was reduced and Pipkin's fracture was fixed using Ganz approach with lag screws; knee joint was reduced closely and tibial plateau fracture was stabilized with lateral buttress plate and a transarticular spanning fixator. The open fracture on the other leg was debrided and fixed with an external fixator. There was no insta-bility in both joints after fixation when he was examined under anesthesia. The fractures united after 3 months and the patient had no residual instability of hip and knee. There was no clinical or radiological evidence of osteonecrosis in the hip joint after 6 months. At one-year follow-up, he had satisfactory functional outcome with almost normal range of motion at both joints. Ipsilateral hip and knee dislocations are rare injuries and more caution is needed for early diagnosis. A timely appropriate intervention can provide good functional outcome to the patient in this situation. 展开更多
关键词 Hip dislocation Knee dislocation FRACTURES bone
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Therapeutic effect of osteogenically induced adipose derived stem cells on vascular deprivation-induced osteonecrosis of the femoral head in rabbits 被引量:2
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作者 Abuclusaimi Aimaiti Yilixiafi Saiwulaiti +3 位作者 Maimaitiming Saiyiti WANG Yun-hai CUI Lei Aihemaitijiang Yusufu 《Chinese Journal of Traumatology》 CAS 2011年第4期215-220,共6页
Objective: To explore the therapeutic ef- fect of osteogenically induced adipose-derived stem cells (ADSCs) on vascular deprivation-induced osteonecrosis of the femoral head (ONFH) in rabbit model. Methods: Vas... Objective: To explore the therapeutic ef- fect of osteogenically induced adipose-derived stem cells (ADSCs) on vascular deprivation-induced osteonecrosis of the femoral head (ONFH) in rabbit model. Methods: Vascular deprivation-induced ONFH was established by intramuscular injection of methylpre- dnisolone, and vascular occlusion of the capital femoral epiphysis by electrocoagulation in adult New Zealand white rabbits. Eight weeks afLer the establishment of vascular dep- rivation-induced ONFH, animals were randomly divided into three equal groups. In Group A (control), no therapy was given. In Group B, core decompression was performed by drilling a hole (1.2 mm in diameter) from the outer cortex 2.5 cm distal to the proximal end of the greater trochanter. In Group C, 1 x 107 osteogenically induced ADSCs were resus- pended in 0.5 ml PBS, and then injected directly into the femoral head. Femoral head specimens were obtained at postoperative 8 weeks. The bone formation and three-di- mensional microstructure of the femoral head was evaluated by micro-computed tomography scans (u-CT). Immunohistochemical analysis was performed to detect the expression ofosteocalcin. Angiogenesis and repair of the femoral head were observed histologically. Results: In trabecular bone at the proximal femur region, the trabecular volume was higher in Group C (130.70 mm3± 4.33 mm3) than that in Groups A (101.07 mm3±7.76 mm3) and B (107.89 mm3±8.6g mm3, P〈0.01). Bone volume was significantly increased in Group C (40.09 mm3±6.35 mm3) than in Groups A (29.65 mm3±4.61 mm3) and B (31.80 mm3± 4.01 mm3, P〈0.01). The trabecular number was higher in Groups C (1.58±0.25) than other two groups (1.15±0.18, 1.16± 0.21, P〈0.01). Bone mineral density showed statistically significant difference between Groups C and A or B (375.38± 23.06) mg HA/ccm, vs (313.73 ± 19.30) mg HA/ccm and (316.09± 16.45) mg HA/ccm, P〈0.01). Histological examination indicated that there was more new bone formation in Group C than in other groups. Conclusion: Treatment with autologous osteogenically induced ADSCs transplantation results in an enhanced osteogenesis and microstructure of the vascular deprivation-induced osteonecrosis in rabbits. 展开更多
关键词 Adipose tissue Stem cells OSTEONECROSIS OSTEOGENESIS Transplantation autologus
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