应用皮肤原位再生医疗技术治疗难愈性创面的经验总结

慢性难愈性创面具有病因复杂、病程长、并发症多等特点,是临床治疗中的棘手问题。此报告介绍了应用皮肤原位再生医疗技术(MEBT/MEBO)治疗慢性难愈性创面的临床治疗情况。临床实践证明,MEBT/MEBO在难愈性创面的治疗中能够无损伤地液化排除坏死组织,通过体细胞转化为干细胞实现创面组织器官的原位再生复原。在较快愈合创面的同时,具有止痛、控制感染、创面愈合质量好等优点,是慢性难愈性创面较理想的治疗方法..下图是临床应用的典型病例图片,详细病例资料请观看本期杂志所附光盘。

皮肤原发间变大细胞淋巴瘤一例

临床资料患者,男,75岁。因躯干及四肢泛发红斑水疱伴瘙痒半年,加重伴双下肢多处溃疡2^＋月入院。患者发病半年前因外伤双下肢出现红斑丘疹水疱,破溃后流黄水,形成糜烂面,逐渐发展至躯干及双上肢,部分红斑丘疹上覆蛎壳状鳞屑,溃疡面上覆黑色坏死样痂皮,就诊于成都多家医院。诊断为＂银屑病＂,治疗效果不佳。患者双下肢水肿明显,溃疡处分泌物增多,故来我院就诊。患者自觉瘙痒疼痛。既往史：有高血压、糖尿病、冠心病。对＂磺胺类药物＂过敏。

《烧伤治疗大全》内容简介——一部系统介绍烧伤皮肤原位再生技术的新书

烧伤湿润暴露疗法皮肤原位再生医疗技术在全国普及推广已经有20多年了，为了满足广大医护人员的需求，由中国科技出版社编辑出版的烧伤再生医学创始人徐荣祥的专著《烧伤治疗大全》已经出版并在全国发行。

金秋十月情谊浓 学术共鸣金陵城——江苏省皮肤原位再生医疗技术临床应用高级学术研讨会纪实

2010年10月9日，六朝古都南京阳光普照秋意正浓，由中国中西医结合学会烧伤专业委员会和江苏省医学会烧伤整形专业委员会联合主办的“皮肤原位再生医疗技术发展与临床应用高级学术研讨会”在毗邻江苏省人民医院的瑞迪大酒店举办。

肤美达医用胶原充填剂对大鼠慢性毒性的试验研究

目的考察肤美达医用胶原充填剂对大鼠的慢性毒性。方法选取健康Wistar大鼠120只,一次给予肤美达医用胶原充填剂样品,观察期为3个月,观察期结束后,进行各组动物的血液学和血清生化指标测定及病理组织学检查。结果经过3个月观察,各组动物活动、毛色、行为、进食量、体质量、尿、便均无明显异常情况,血液学和血清生化指标皆在正常范围内,各组未见脏器组织有明显器质性异常改变。结论肤美达医用胶原充填剂,在350、175、87.5 mg/kg剂量下对大鼠无慢性毒性作用。

肤可新医用胶原复配型凝胶敷料和胶原贴敷料在面部激光术后应用效果探讨

目的探讨肤可新医用胶原复配型凝胶敷料和胶原贴敷料在面部激光术后应用的效果,为进一步改善患者预后提供更为有效的方法。方法回顾性选取2018年1月—2020年1月广东省广州市皮肤病防治所进行面部激光术的100例患者,分为两组,联合治疗组(n=50)采用肤可新医用胶原复配型凝胶敷料和胶原贴敷料联合治疗,常规冰敷组(n=50)采用常规冰敷治疗。分析两组患者的皮肤症状评分、术后创面渗出时间、红斑、疼痛持续时间、水肿时间、脱痂时间、自我感觉舒适度、满意度、临床疗效。结果联合治疗组患者的疼痛、干燥、脱屑评分均低于常规冰敷组,差异有统计学意义(P<0.05);术后创面渗出时间,红斑、疼痛持续时间,水肿时间,脱痂时间均显著短于常规冰敷组;减轻创面痛痒觉及提高创面平整度、色泽、外观满意度均显著高于常规冰敷组,差异有统计学意义(P<0.05)。联合治疗组患者的自我感觉舒适度(88.0%)显著高于常规冰敷组患者的自我感觉舒适度(72.0%),差异有统计学意义(χ^(2)=4.000,P<0.05)。联合治疗组患者治疗的总有效率显著高于常规冰敷组,差异有统计学意义(P<0.05)。结论肤可新医用胶原复配型凝胶敷料和胶原贴敷料联合在面部激光术后应用效果较常规冰敷好。

猪肤胶原蛋白肽脂质体的制备及其质量评价

目的优化猪肤胶原蛋白脂质体制备方案,并进行质量评价。方法以脂质体的包封率为评价指标,采用单因素实验筛选出影响脂质体包封率的3大因素,用正交实验确定最佳制备工艺。结果胶原蛋白多肽脂质体制备最佳条件:药物与胆固醇、磷脂的比例为3∶8∶24、磷酸盐缓冲液体积为20mL、温度为40℃、超声时间为30min。结论用此方法制备猪肤胶原蛋白多肽脂质体,质量稳定,符合相关要求。

人尿激肽原酶治疗急性脑梗死的临床观察

目的:观察人尿激肽原酶治疗急性脑梗死的近期疗效和安全性。方法:应用人尿激肽原酶0.15 PNAU·d^(-1)×7 d。治疗45例急性脑梗死患者,比较治疗前后NIHSS评分、mRS评分,并观察治疗期间不良反应。结果:①治疗前、治疗7d后患者NIHSS评分、mRS评分明显改善;②2例出现血压明显下降。结论:人尿激肽原酶治疗急性脑梗死疗效明显;安全性较高;在用药期间需注意观察血压的变化。

六味地黄丸合胰激肽原酶干预高血压病肾损害的疗效观察

将 75例阴虚阳亢型原发性高血压病患者 ,随机分为治疗组和对照组 ,治疗组加服六味地黄丸和胰激肽原酶 ,连续治疗 1 2周 ,比较 2组疗效、微量白蛋白尿 ( m Alb)和肾小球滤过率 ( GFR)情况。结果显示治疗组能显著改善临床症状 ,2组均能显著升高 GFR、降低 m Alb,2组疗效比较有非常显著意义 ( P<0 .0 1 ) ,说明六味地黄丸合胰激肽原酶能够减轻阴虚阳亢型高血压病患者的肾损害 ,同时又能改善临床症状 ,提高疗效。

羧甲基果聚糖的胶原肽修饰及其活性研究

为考察羧甲基果聚糖及其衍生物在功能性食品、生物医学及化妆品方面的应用可行性,通过羧基与氨基的交联反应制备了胶原肽修饰的羧甲基果聚糖,并测试了产物的抗氧化、吸湿保湿活性以及其对人皮角质Ha Ca T细胞生长增殖的影响。发现羧甲基果聚糖经胶原肽修饰后抗氧化性明显提高,25 mg/m L时的ABTS^+·清除率为62.31%,比修饰前提高了6.11倍。尽管胶原肽的引入会对吸湿保湿性造成影响,修饰后的羧甲基果聚糖仍然保留良好的吸湿保湿性能。在相对湿度43%和81%中放置72 h的吸湿率分别为11.96%和21.54%,在干燥硅胶环境中放置72 h的保湿率为60.48%。此外,修饰产物在0~1 mg/m L的浓度范围内无细胞毒性,可以促进Ha Ca T细胞增殖。Ha Ca T细胞在0.5 mg/m L修饰产物刺激下的存活率为124.2%。胶原肽修饰能提高羧甲基果聚糖的生物活性,拓展其在功能性食品、医药制品和化妆品上的应用潜力。

Changes of the Transcriptional Levels of Molecules Associated with Endogenous Antigen Processing and Presentation in Porcine Skin-derived Dendritic Cells Infected with PCV2 in vivo

[Objective] This study aimed to investigate the changes of the transcriptional levels of molecules associated with endogenous antigen processing and presenta- tion in porcine skin-derived dendritic cells infected with PCV2 in vivo. [Method] Healthy 40-day-old Landrace piglets were infected with porcine circovirus type 2 （PCV2） and euthanized on the 34, 7rd, 14th, 21st and 35th d post inoculation （DPI）. The porcine skin-derived dendritic cells （DCs） were collected to analyze the transcrip- tional levels of molecules （LMP7, UBP, MHC-I, calreticulin） associated with endogenous antigen processing and presentation by using real-time fluorescent quantitative PCR （real-time FQ-PCR）. [Result] The results showed that the level of LMP7 mR- NAs was reduced significantly on the 3DPI （P〈0.05）; the level of UBP mRNAs was consistently up-regulated, which increased significantly on the 21DPI and 35DPI （P〈 0.05）; the level of MHC-I mRNAs was significantly down-regulated on the 7DPI （P〈 0.05）; the level of calreticulin mRNAs was up-regulated slightly without significant dif- ference. [Conclusion] PCV2 can inhibit the endogenous antigen processing and presentation ability of porcine skin-derived DCs at early stages of infection.

The Polypeptide in Chlamys farreri can protect human dermal fibroblasts from ultraviolet B damage

To investigate the effect of polypeptide from Chlamys farreri (PCF) on NHDF in vitro, we modeled oxidative damage on normal human dermal fibroblasts (NHDF) exposed to ultraviolet B (UVB). In this study, 3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) and lactate dehydro-genase (LDH) were tested to measure cell viability. Enzymes including superoxide dismutase (SOD), glu-tathione peroxidase (GSH-PX), catalase (CAT) and xanthine oxidase (XOD) were determined biochemically. Total antioxidative capacity (T-AOC) and anti-superoxide anion capacity (A-SAC) were also determined. Ultrastructure of fibroblasts was observed under transmission electron microscope. The results showed that: UVB (1.176×10-4 J/cm2) suppressed the growth of fibroblasts and the introduction of PCF (0.25%-l%) before UVB reduced the suppression in a concentration-dependent manner. PCF could enhance the activities of SOD, GSH-PX and T-AOC as well as A-SAC. Also PCF could inhibit XOD activity, while it did not affect CAT activity. Ultrastructure of fibroblasts were damaged after UVB irradiation, concentration-dependent PCF reduced the destructive effect of UVB on cells. These results indicated that PCF can protect human dermal fibroblasts from being harmed by UVB irradiation via its antioxidant pro-erty.

THE FIRST CASE OF PROTOTHECOSIS ZOPFII IN CHINA

Objective. Report of first case of Protothecosis zopfii in China and causes the skin infection in the world. Method.By clinical and laboratory examinations to confirm the diagnosis and the response to treatment. By the review of literatures to confirm the first case of human skin infection in the world. Result.From the literatures and the clinical pictures, it is confirmed that this is the first case report of Protothecosis zopfii of skin in the world. Conclusion.The first case of Protothecosis zopfii in human being was reported and successfully treated with local infiltration of Diflucan (fluconazole) 3ml (2mg/ml)/week×4.

A COMPARATIVE STUDY OF GUT SUTURE, HUMAN AMNION COLLAGEN, BOVINE SKIN COLLAGEN AND VICRYL SUTURE IMPLANTS IN RATS

This study compares the persistence and histological characteristics of gut suture with those of human amnion collagen, bovine collagen, and Vicryl suture implants in rats. Gut suture and human amnion collagen more resembled living tissue than did bovine collagen and were characterized by their cel1ularity and the presence of numerous capillaries. The Vicryl suture implants were quickly absorbed. Picrosirius polarization revealed the synthesis of host collagen by rat fibroblasts which immigrated into the gut suture and human amnion collagen implants. The authors suggest the potential of gut suture as a soft tissue substitute to improve linear skin contour deficits.

Induced Immunity of Response to Some Allergens Towards Response of Other Skin Test Allergens and Its Correlation with Immediate Hypersensitivity Reactions

Allergens are innocuous environmental antigens that cause lgE mediated hypersensitivity reactions. These antigens can be divided into several groups according to their nature like plant allergens, grasses, fungal allergens, pollen grains and house dust mite. Skin response to such allergens is associated with each other either by positive relation （positive correlation） or negative （inverse） relation. No previous papers found to discuss such correlations. In order to investigate these correlations, skin prick test of standard allergens solutions was carried out in 500 patients suffering allergic reactions of the age 12-60 years of both sexes. The results showed that there is a high significance of positive and negative correlations in skin response to allergens in the same group and with allergens of different groups suggesting that there is an induction or suppression of immune response. In conclusion the similarity or dissimilarity in the primary structure of some allergens in addition to gene-environment interaction may reflect positive/negative correlations between certain allergens that can influence the related allergic reactions.

Characterization and antioxidant activities of marine pepsin soluble collagen from the skin of yellow goosefish Lophius litulon

Characteristics and antioxidant activities of pepsin-soluble collagen （PSC） from yellow goosefish （Lophius litulon） skins were investigated. PSC was characterized as a type I collagen, and its imino acid content was 193 residues/1 000 residues. PSC＇s denaturation temperature was -17.56℃ and Fourier transform infrared spectra confirmed the presence of triple helices. Solubility analysis showed good solubility at acidic pH （1-6） or low NaCl concentrations （≤2%）. PSC showed scavenging activity against hydroxyl radicals and superoxide anions in a concentration-dependent manner. Furthermore, PSC could protect D-galactose-induced skin aging by significantly controlling malondialdehyde formation and improving the activity of superoxide dismutase, glutathione peroxidase, catalase, glutathione, and hydroxyproline. PSC may be a promising antioxidant in appropriate applications.

EFFECTS OF SDS,PBS SOLUTIONS UPON FLUORESCENCE VALUES OF DERMATOPHAGOIDES PTERRONYSSINUS RADIOALLERGOSORBENT TEST INHIBITION

Objective To explore the effects of SDS, PBS re-dissolvent solutions on fluorescence values of radioallergosorbent test (RAST) inhibition. Methods Dermatophagoides pterronyssinus allergen immunoCAP and UniCAP 100 System were used. The Sera Pool consisted of 20 Dermatophagoides pterronyssinus allergic patients sera, their specific IgE fluorescence values were between 12 505 and 24 776. Results Fluorescence value percentages decreased: 62.9%, 54.1%, 43.5%, 6.7%, 3.7%, 2.6%, 2.2%, and 1.4% respectively, when SDS concentrations were at 2%, 1%, 0.5%, 0.25%, 0.1%, 0.05%, 0.025%, and 0.01%. Fluorescence values decreased more than 5% with SDS concentrations equal to 0.25% or higher. PBS in 0.1 and 0.01 mol/L concentrations decreased fluorescence values 2.9% and 0.9% respectively. Conclusions SDS is a commonly used surfactants in allergen extract and re-dissolvent prepared allergen precipitation for RAST inhibition. Thus effects of surfactants (e.g. SDS) upon the RAST inhibition tests must be considered when they were used as re-dissolvent agents to improve protein resolution in RAST inhibition.

正畸初期矫治力对大鼠三叉神经节PPTA mRNA变化的影响

目的观察正畸初期不同矫治力对三叉神经节中PPTA mRNA(Preprotachykinin-A,前速激肽原A)的影响。方法采用反转录-聚合酶链反应(RT-PCR)技术检测来观察正畸初期阶段不同正畸力值作用下大鼠三叉神经节中PPTA mRNA的变化情况。结果实验组大鼠三叉神经节PPTA mRNA的表达发生了明显的变化,并呈现一定的规律性。加力2h PPTA mRNA的表达量开始增加;加力3d达到最高;之后逐渐下降,但7天仍然高于对照组(P<0.05);不同正畸力组之间,20g组和50g组大鼠三叉神经节PPTA mRNA的动态变化相似,各时间点两组之间无统计学差异(P>0.05);80g组在各个时间点PPTA mRNA的表达明显高于其它两组(P<0.05),并且峰值有相对提前的趋势。结论本研究提示正畸初期力值的大小对疼痛的表达可能有不同程度的影响。