The bread-making quality of wheat is a highly complex trait that depends on both genetic and environmental factors. This study aims at evaluating the effects of different rates, time and splitting of nitrogen fertiliz...The bread-making quality of wheat is a highly complex trait that depends on both genetic and environmental factors. This study aims at evaluating the effects of different rates, time and splitting of nitrogen fertilization on the technological quality of wheat cultivated in the Brazilian Southern region. The samples of bread wheat （Triticum aestivum L.）, Onix, Quartzo and Mirante cultivars, were obtained through the use of nitrogen （N） fertilizer applied in doses of 36, 100 and 120 kg N ha1 at sowing, tillering and flowering. Laboratorial tests were carried out in a completely randomized design with four repetitions. The parameters analyzed were： grain yield, total protein, protein fractions, gliadins, glutenins, albumins and globulins, sulfur, gluten strength （W）, dough tenacity （P）, extensibility （L） and stability （S）, bread specific volume and bread firmness. While the content of total and reserve proteins is significantly increased with a higher rate and splitting of N, the content of metabolic proteins remains constant. A mean increase in the quality parameters W （24.37%）, L （14.86%） and P （11.59%） among cultivars was noticed after application of 120 kg N ha1, split at sowing, tillering and flowering. Bread specific volume increased, while bread firmness decreased with a higher rate of N fertilizer. Wheat fertilization with high doses of N does not cause induction to S deficiency in the grains. Not only increasing the N fertilization rate, but also splitting the N rate had a beneficial effect on the technological quality of wheat.展开更多
AIM To identify the factors influencing cecal insertion time(CIT) and to evaluate the effect of obesity indices on CIT. METHODS We retrospectively reviewed the data for participants who received both colonoscopy and a...AIM To identify the factors influencing cecal insertion time(CIT) and to evaluate the effect of obesity indices on CIT. METHODS We retrospectively reviewed the data for participants who received both colonoscopy and abdominal computed tomography(CT) from February 2008 to May 2008 as part of a comprehensive health screening program. Age, gender, obesity indices [body mass index(BMI), waist-to-hip circumference ratio(WHR), waist circumference(WC), visceral adipose tissue(VAT)volume and subcutaneous adipose tissue(SAT) volume on abdominal CT], history of prior abdominal surgery, constipation, experience of the colonoscopist, quality of bowel preparation, diverticulosis and time required to reach the cecum were analyzed. CIT was categorized as longer than 10 min(prolonged CIT) and shorter than or equal to 10 min, and then the factors that required a CIT longer than 10 min were examined.RESULTS A total of 1678 participants were enrolled. The mean age was 50.42 ± 9.931 years and 60.3% were men. The mean BMI, WHR, WC, VAT volume and SAT volume were 23.92 ± 2.964 kg/m2, 0.90 ± 0.076, 86.95 ± 8.030 cm, 905.29 ± 475.220 cm3 and 1707.72 ± 576.550 cm3, respectively. The number of patients who underwent abdominal surgery was 268(16.0%). Colonoscopy was performed by an attending physician alone in 61.9% of cases and with the involvement of a fellow in 38.1% of cases. The median CIT was 7 min(range 2-56 min, IQR 5-10 min), and mean CIT was 8.58 ± 5.291 min. Being female, BMI, VAT volume and involvement of fellow were significantly associated with a prolonged CIT in univariable analysis. In multivariable analysis, being female(OR = 1.29, P = 0.047), lower BMI(< 23 kg/m2)(OR = 1.62, P = 0.004) or higher BMI(≥ 25 kg/m2)(OR = 1.80, P < 0.001), low VAT volume(< 500 cm3)(OR = 1.50, P = 0.013) and fellow involvement(OR = 1.73, P < 0.001) were significant predictors of prolonged CIT. In subgroup analyses for gender, lower BMI or higher BMI and fellow involvement were predictors for prolonged CIT in both genders. However, low VAT volume was associated with prolonged CIT in only women(OR = 1.54, P = 0.034).CONCLUSION Being female, having a lower or higher BMI than the normal range, a low VAT volume, and fellow involvement were predictors of a longer CIT.展开更多
Biologically active soil organic carbon （BASOC） is an important fraction of soil organic carbon （SOC）, but our understanding of the correlation between BASOC and soil aggregate stability is limited. At an ecologic...Biologically active soil organic carbon （BASOC） is an important fraction of soil organic carbon （SOC）, but our understanding of the correlation between BASOC and soil aggregate stability is limited. At an ecological experimental station （28° 04＇-28° 37＇ N, 116° 41＇-117° 09＇ E） in Yujiang County, Jiangxi Province, China, we analyzed the dynamic relationship between soil aggregate stability and BASOC content over time in the red soil （Udic Ferrosols） fertilized with a nitrogen-phosphorus-potassium chemical fertilizer （NPK） without manure or with NPK plus livestock manure or green manure. The dynamics of BASOC was evaluated using CO2 efflux, and soil aggregates were separated according to size using a wet-sieving technique. The soils fertilized with NPK plus livestock manure had a significantly higher content of BASOC and an improved aggregate stability compared to the soils fertilized with NPK plus green manure or NPK alone. The BASOC contents in all fertilized soils decreased over time. The contents of large aggregates （800-2 000 μm） dramatically decreased over the first 7 d of incubation, but the contents of small aggregates （〈 800 μm） either remained the same or increased, depending on the incubation time and specific aggregate sizes. The aggregate stability did not differ significantly at the beginning and end of incubation, but the lowest stability in all fertilized soils occurred in the middle of the incubation, which implied that the soils had a strong resilience for aggregate stability. The change in BASOC content was only correlated with aggregate stability during the first 27 d of incubation.展开更多
It is important to understand the effects of temporal changes in microbial communities in the acidic soils of tea orchards with different fertilizers. A field experiment involving organic fertilizer （OF）, chemical f...It is important to understand the effects of temporal changes in microbial communities in the acidic soils of tea orchards with different fertilizers. A field experiment involving organic fertilizer （OF）, chemical fertilizer （CF）, and unfertilized control （CK） treatments was arranged to analyze the temporal changes in the bacterial and archaeal communities at bimonthly intervals based on the 16S ribosomal RNA （rRNA） gene using terminal restriction fragment length polymorphism （T-RFLP） profiling. The abundances of total bacteria, total archaea, and selected functional genes （bacterial and archaeal amoA, bacterial narG, nirK, nirS, and nosZ） were determined by quantitative poly- merase chain reaction （qPCR）. The results indicate that the structures of bacterial and archaeal communities varied significantly with time and fertilization based on changes in the relative abundance of dominant T-RFs. The abundancy of the detected genes changed with time. The total bacteria, total archaea, and archaeal amoA were less abundant in July. The bacterial amoA and denitrifying genes were less abundant in September, except the nirK gene. The OF treatment increased the abundance of the observed genes, while the CF treatment had little influence on them. The soil temperature significantly affected the bacterial and archaeal community structures. The soil moisture was signif- icantly correlated with the abundance of denitrifying genes. Of the soil chemical properties, soil organic carbon was the most important factor and was significantly correlated with the abundance of the detected genes, except the nirK gene. Overall, this study demonstrated the effects of both temporal alteration and organic fertilizer on the structures of mi- crobial communities and the abundance of genes involved in the nitrogen cycle.展开更多
文摘The bread-making quality of wheat is a highly complex trait that depends on both genetic and environmental factors. This study aims at evaluating the effects of different rates, time and splitting of nitrogen fertilization on the technological quality of wheat cultivated in the Brazilian Southern region. The samples of bread wheat （Triticum aestivum L.）, Onix, Quartzo and Mirante cultivars, were obtained through the use of nitrogen （N） fertilizer applied in doses of 36, 100 and 120 kg N ha1 at sowing, tillering and flowering. Laboratorial tests were carried out in a completely randomized design with four repetitions. The parameters analyzed were： grain yield, total protein, protein fractions, gliadins, glutenins, albumins and globulins, sulfur, gluten strength （W）, dough tenacity （P）, extensibility （L） and stability （S）, bread specific volume and bread firmness. While the content of total and reserve proteins is significantly increased with a higher rate and splitting of N, the content of metabolic proteins remains constant. A mean increase in the quality parameters W （24.37%）, L （14.86%） and P （11.59%） among cultivars was noticed after application of 120 kg N ha1, split at sowing, tillering and flowering. Bread specific volume increased, while bread firmness decreased with a higher rate of N fertilizer. Wheat fertilization with high doses of N does not cause induction to S deficiency in the grains. Not only increasing the N fertilization rate, but also splitting the N rate had a beneficial effect on the technological quality of wheat.
基金Supported by National Cancer Center,South Korea,No.NCC-1610250,No.NCC-1410250,and No.NCC 0810200-1
文摘AIM To identify the factors influencing cecal insertion time(CIT) and to evaluate the effect of obesity indices on CIT. METHODS We retrospectively reviewed the data for participants who received both colonoscopy and abdominal computed tomography(CT) from February 2008 to May 2008 as part of a comprehensive health screening program. Age, gender, obesity indices [body mass index(BMI), waist-to-hip circumference ratio(WHR), waist circumference(WC), visceral adipose tissue(VAT)volume and subcutaneous adipose tissue(SAT) volume on abdominal CT], history of prior abdominal surgery, constipation, experience of the colonoscopist, quality of bowel preparation, diverticulosis and time required to reach the cecum were analyzed. CIT was categorized as longer than 10 min(prolonged CIT) and shorter than or equal to 10 min, and then the factors that required a CIT longer than 10 min were examined.RESULTS A total of 1678 participants were enrolled. The mean age was 50.42 ± 9.931 years and 60.3% were men. The mean BMI, WHR, WC, VAT volume and SAT volume were 23.92 ± 2.964 kg/m2, 0.90 ± 0.076, 86.95 ± 8.030 cm, 905.29 ± 475.220 cm3 and 1707.72 ± 576.550 cm3, respectively. The number of patients who underwent abdominal surgery was 268(16.0%). Colonoscopy was performed by an attending physician alone in 61.9% of cases and with the involvement of a fellow in 38.1% of cases. The median CIT was 7 min(range 2-56 min, IQR 5-10 min), and mean CIT was 8.58 ± 5.291 min. Being female, BMI, VAT volume and involvement of fellow were significantly associated with a prolonged CIT in univariable analysis. In multivariable analysis, being female(OR = 1.29, P = 0.047), lower BMI(< 23 kg/m2)(OR = 1.62, P = 0.004) or higher BMI(≥ 25 kg/m2)(OR = 1.80, P < 0.001), low VAT volume(< 500 cm3)(OR = 1.50, P = 0.013) and fellow involvement(OR = 1.73, P < 0.001) were significant predictors of prolonged CIT. In subgroup analyses for gender, lower BMI or higher BMI and fellow involvement were predictors for prolonged CIT in both genders. However, low VAT volume was associated with prolonged CIT in only women(OR = 1.54, P = 0.034).CONCLUSION Being female, having a lower or higher BMI than the normal range, a low VAT volume, and fellow involvement were predictors of a longer CIT.
基金Supported by the National Natural Science Foundation of China (No.30971869)the Knowledge Innovation Program of the Chinese Academy of Sciences (No.KZCX2-YW-438-1)
文摘Biologically active soil organic carbon （BASOC） is an important fraction of soil organic carbon （SOC）, but our understanding of the correlation between BASOC and soil aggregate stability is limited. At an ecological experimental station （28° 04＇-28° 37＇ N, 116° 41＇-117° 09＇ E） in Yujiang County, Jiangxi Province, China, we analyzed the dynamic relationship between soil aggregate stability and BASOC content over time in the red soil （Udic Ferrosols） fertilized with a nitrogen-phosphorus-potassium chemical fertilizer （NPK） without manure or with NPK plus livestock manure or green manure. The dynamics of BASOC was evaluated using CO2 efflux, and soil aggregates were separated according to size using a wet-sieving technique. The soils fertilized with NPK plus livestock manure had a significantly higher content of BASOC and an improved aggregate stability compared to the soils fertilized with NPK plus green manure or NPK alone. The BASOC contents in all fertilized soils decreased over time. The contents of large aggregates （800-2 000 μm） dramatically decreased over the first 7 d of incubation, but the contents of small aggregates （〈 800 μm） either remained the same or increased, depending on the incubation time and specific aggregate sizes. The aggregate stability did not differ significantly at the beginning and end of incubation, but the lowest stability in all fertilized soils occurred in the middle of the incubation, which implied that the soils had a strong resilience for aggregate stability. The change in BASOC content was only correlated with aggregate stability during the first 27 d of incubation.
基金supported by the Research Fund for the Doctoral Program of Higher Education of China(No.20130101110127)the Project of Zhejiang Key Scientific and Technological Innovation Team(No.2010R50039),China
文摘It is important to understand the effects of temporal changes in microbial communities in the acidic soils of tea orchards with different fertilizers. A field experiment involving organic fertilizer （OF）, chemical fertilizer （CF）, and unfertilized control （CK） treatments was arranged to analyze the temporal changes in the bacterial and archaeal communities at bimonthly intervals based on the 16S ribosomal RNA （rRNA） gene using terminal restriction fragment length polymorphism （T-RFLP） profiling. The abundances of total bacteria, total archaea, and selected functional genes （bacterial and archaeal amoA, bacterial narG, nirK, nirS, and nosZ） were determined by quantitative poly- merase chain reaction （qPCR）. The results indicate that the structures of bacterial and archaeal communities varied significantly with time and fertilization based on changes in the relative abundance of dominant T-RFs. The abundancy of the detected genes changed with time. The total bacteria, total archaea, and archaeal amoA were less abundant in July. The bacterial amoA and denitrifying genes were less abundant in September, except the nirK gene. The OF treatment increased the abundance of the observed genes, while the CF treatment had little influence on them. The soil temperature significantly affected the bacterial and archaeal community structures. The soil moisture was signif- icantly correlated with the abundance of denitrifying genes. Of the soil chemical properties, soil organic carbon was the most important factor and was significantly correlated with the abundance of the detected genes, except the nirK gene. Overall, this study demonstrated the effects of both temporal alteration and organic fertilizer on the structures of mi- crobial communities and the abundance of genes involved in the nitrogen cycle.
