[Objective]The aim was to discuss the actinomycete biodiversity of iron-mine tailings by phylogenetic analysis of 12 typical isolates. [Method]The genomic DNAs were extracted by phenol-chloroform method; phylogeny ana...[Objective]The aim was to discuss the actinomycete biodiversity of iron-mine tailings by phylogenetic analysis of 12 typical isolates. [Method]The genomic DNAs were extracted by phenol-chloroform method; phylogeny analysis was carried out based on 16S rDNA PCR amplification and sequencing. [Result]The results showed that all the 12 strains belong to the genus Streptomyces sharing 98.7%-99.9% similarities with their nearest known neighbors. [Conclusion]Streptomyces is the dominant culturable actinomycete group of iron mine tailings,in which there are many potential novel species.展开更多
[Objective] The aim of this study was to analyze the cytoplasmic male sterile line 21A and its maintainer line 21B of peppers by AFLP,and lay the foundation for further studies on molecular mechanism of the cytoplasmi...[Objective] The aim of this study was to analyze the cytoplasmic male sterile line 21A and its maintainer line 21B of peppers by AFLP,and lay the foundation for further studies on molecular mechanism of the cytoplasmic male sterility in peppers.[Method] Cytoplasmic male sterility(CMS)line 21A and its maintainer line 21B were analyzed by AFLP to obtain the specific amplified fragments of cytoplasmic male sterile line 21A,while the specific amplified fragments were recovered or sequenced,and analyzed by BLAST...展开更多
Pentatomomorpha is one of the most biodiverse infraorders among the true bugs(Hemiptera: Heteroptera). Phylogenetic relationships among the superfamilies within this infraorder have been uncertain, especially for t...Pentatomomorpha is one of the most biodiverse infraorders among the true bugs(Hemiptera: Heteroptera). Phylogenetic relationships among the superfamilies within this infraorder have been uncertain, especially for the Eutrichophora. The previous studies were based on morphological characters, or just mitochondrial or nuclear genes, or only partial 18 S rDNA and COI. In this study, we used maximum likelihood(ML) and Bayesian inference(BI) based on massive molecular datasets(18S rDNA, 28 S rDNA, Hox and mitochondrial genes totaling 21 loci and 12,538 characters) to infer a robust phylogeny for this terrestrial group. Results strongly support the monophyly of all superfamilies; the superfamily status of Aradoidea and the following relationships:(Aradoidea +(Pentatomoidea +(Coreoidea +(Lygaeoidea + Pyrrhocoroidea)))) in Pentatomomorpha, and(Coreoidea +(Lygaeoidea + Pyrrhocoroidea)) in Eutrichophora. Our results suggest that sampling greater numbers of genes is an effective tool for resolving phylogenetic problems.展开更多
[Objective] The aim was to isolate the strains resistant to plant pathogenic fungi from Southern Ocean and study their phylogenetic relationship and antimicrobial spectrum. [Method] Agar diffusion method was adopted t...[Objective] The aim was to isolate the strains resistant to plant pathogenic fungi from Southern Ocean and study their phylogenetic relationship and antimicrobial spectrum. [Method] Agar diffusion method was adopted to screen antimicrobial strains and determine the antimicrobial spectrum. Phylogenetic relationship of the strains was analyzed by neighbor-joining method of the Mega 4.0 software. [Result] Twenty antimicrobial strains were screened from seawater of Southern Ocean collected during the 27^th Chinese Antarctic Scientific Expedition. Molecular identification and phyloge- netic analysis indicated that two antimicrobial strains were members of Pseu- domonas, two strains were members of Psychrobacter, and the other 16 trains were members of Pseudoalteromonas. The antimicrobial spectrum of four strains which had higher antimicrobial activity indicated that the strains 312, 83-1 and 195 greatly inhibited the growth of Fusarium oxysporum, Rhizoctonia solani K(Jhn, Phytophthora capsici Leonian, Verticillium dahliae, Alternaria solani, Thanatephoru scucumeris and Phomopsis asparagi (Sacc); strain 312-1 had obvious antimicrobial effect on the six of the plant pathogens except R. solani. [Conclusion] Four strains which had higher antimicrobial effect were obtained and should be further studied for development and application.展开更多
[Objective] The research aimed at cloning and analyzing mitochondrial cytochrome oxidase I gene(cox 1)of C.suppressalis.[Method] The mitochondrial cox 1 gene of C.suppressalis was cloned with PCR method and sequence...[Objective] The research aimed at cloning and analyzing mitochondrial cytochrome oxidase I gene(cox 1)of C.suppressalis.[Method] The mitochondrial cox 1 gene of C.suppressalis was cloned with PCR method and sequenced.Then,cox1 sequences of other 21 Lepidopteran species were obtained by blasting the GenBank with cox 1 gene sequence of C.suppressalis.Finally,homology comparison and molecular phylogenitic analysis among the 22 Lepidopteran species were conducted.[Result] The open reading frame of cox 1 gene from C.suppressalis contained 1 531 nucleotides encoding a putative protein of 510 amino acids.The cox1 gene used a start codon CGA,and an incomplete termination codon composed of only T.Based on the amino acid sequences of cox 1,the molecular phylogenetic tree of Lepidoptera was reconstructed using the maximum likelihood(ML)method.The molecular phylogenetic tree was similar to the morphological phylogenetic tree mainly,but also showed some differences.[Conclusion] The result will provide reference for further research on expression and application of cox 1 gene.展开更多
基金Supported by National Natural Science Foundation of China(30800003)Provincial Foundation Advanced Project of Hebei Uni-versity (2006Y09)~~
文摘[Objective]The aim was to discuss the actinomycete biodiversity of iron-mine tailings by phylogenetic analysis of 12 typical isolates. [Method]The genomic DNAs were extracted by phenol-chloroform method; phylogeny analysis was carried out based on 16S rDNA PCR amplification and sequencing. [Result]The results showed that all the 12 strains belong to the genus Streptomyces sharing 98.7%-99.9% similarities with their nearest known neighbors. [Conclusion]Streptomyces is the dominant culturable actinomycete group of iron mine tailings,in which there are many potential novel species.
基金Supported by National 863 Program in the 11th Five-year Planning Period(2006AA100108-3-2)National Natural Science Foundation(30860120+2 种基金30900781)Science and Technology Project from Jiangxi Provincial Office of Education(GJJ08159)Young Growth Fund of Jiangxi Normol University(2398)~~
文摘[Objective] The aim of this study was to analyze the cytoplasmic male sterile line 21A and its maintainer line 21B of peppers by AFLP,and lay the foundation for further studies on molecular mechanism of the cytoplasmic male sterility in peppers.[Method] Cytoplasmic male sterility(CMS)line 21A and its maintainer line 21B were analyzed by AFLP to obtain the specific amplified fragments of cytoplasmic male sterile line 21A,while the specific amplified fragments were recovered or sequenced,and analyzed by BLAST...
基金supported by the National Natural Science Foundation of China (31372240, 31501840, 31440078)the Postdoctoral Science Foundation of China (134845)the Taiyuan Normal University Training Program of Innovation and Entrepreneurship for Undergraduates (CXCY1610)
文摘Pentatomomorpha is one of the most biodiverse infraorders among the true bugs(Hemiptera: Heteroptera). Phylogenetic relationships among the superfamilies within this infraorder have been uncertain, especially for the Eutrichophora. The previous studies were based on morphological characters, or just mitochondrial or nuclear genes, or only partial 18 S rDNA and COI. In this study, we used maximum likelihood(ML) and Bayesian inference(BI) based on massive molecular datasets(18S rDNA, 28 S rDNA, Hox and mitochondrial genes totaling 21 loci and 12,538 characters) to infer a robust phylogeny for this terrestrial group. Results strongly support the monophyly of all superfamilies; the superfamily status of Aradoidea and the following relationships:(Aradoidea +(Pentatomoidea +(Coreoidea +(Lygaeoidea + Pyrrhocoroidea)))) in Pentatomomorpha, and(Coreoidea +(Lygaeoidea + Pyrrhocoroidea)) in Eutrichophora. Our results suggest that sampling greater numbers of genes is an effective tool for resolving phylogenetic problems.
基金Supported by Public Science and Technology Research Projects of Ocean (201005032-2)~~
文摘[Objective] The aim was to isolate the strains resistant to plant pathogenic fungi from Southern Ocean and study their phylogenetic relationship and antimicrobial spectrum. [Method] Agar diffusion method was adopted to screen antimicrobial strains and determine the antimicrobial spectrum. Phylogenetic relationship of the strains was analyzed by neighbor-joining method of the Mega 4.0 software. [Result] Twenty antimicrobial strains were screened from seawater of Southern Ocean collected during the 27^th Chinese Antarctic Scientific Expedition. Molecular identification and phyloge- netic analysis indicated that two antimicrobial strains were members of Pseu- domonas, two strains were members of Psychrobacter, and the other 16 trains were members of Pseudoalteromonas. The antimicrobial spectrum of four strains which had higher antimicrobial activity indicated that the strains 312, 83-1 and 195 greatly inhibited the growth of Fusarium oxysporum, Rhizoctonia solani K(Jhn, Phytophthora capsici Leonian, Verticillium dahliae, Alternaria solani, Thanatephoru scucumeris and Phomopsis asparagi (Sacc); strain 312-1 had obvious antimicrobial effect on the six of the plant pathogens except R. solani. [Conclusion] Four strains which had higher antimicrobial effect were obtained and should be further studied for development and application.
基金Supported by New Century Program for Excellent Talents of Ministry of Education of China(NCET-07-0251)Talents Foundation of Anhui Province(08040106803)~~
文摘[Objective] The research aimed at cloning and analyzing mitochondrial cytochrome oxidase I gene(cox 1)of C.suppressalis.[Method] The mitochondrial cox 1 gene of C.suppressalis was cloned with PCR method and sequenced.Then,cox1 sequences of other 21 Lepidopteran species were obtained by blasting the GenBank with cox 1 gene sequence of C.suppressalis.Finally,homology comparison and molecular phylogenitic analysis among the 22 Lepidopteran species were conducted.[Result] The open reading frame of cox 1 gene from C.suppressalis contained 1 531 nucleotides encoding a putative protein of 510 amino acids.The cox1 gene used a start codon CGA,and an incomplete termination codon composed of only T.Based on the amino acid sequences of cox 1,the molecular phylogenetic tree of Lepidoptera was reconstructed using the maximum likelihood(ML)method.The molecular phylogenetic tree was similar to the morphological phylogenetic tree mainly,but also showed some differences.[Conclusion] The result will provide reference for further research on expression and application of cox 1 gene.