[Objective] The aim was to study the reproduction of the three-line genic male sterile （GMS） lineparent Mian7MB-1 （B. NapusL.） and the seed production of F1 through somatic tissue culture. [Methed] Through hybridi...[Objective] The aim was to study the reproduction of the three-line genic male sterile （GMS） lineparent Mian7MB-1 （B. NapusL.） and the seed production of F1 through somatic tissue culture. [Methed] Through hybridization, a new breeding material Mian 7MB-1 in three-line genic temporary maintainer line propagated by tissue culture was used to improve the sterile plant rate of rapeseed in dual-purpose recessive GMS line, such as Mian 7AB type, S45AB type, and etc. And then the variety comparative test was performed. [Result] In order to avoid some fertility restoration phenomena occurring during the process of self-reproduction, Mian 7AB was propagated in bulk with somatic tissue culture of temporary maintainer line plant stem. The propagated temporary maintainer line seedlings were applied to the breeding and seed production of net room male sterile line parent, promoting the sterile plant rate of the male sterile line parent to 91.7% -93.5%. The male sterile line parents per hectare were enough for the seed production of hybrid F1 in 7 500 -15 000 hm^2. [ Conclusion ] Compared with the original dual-purpose GMS line, the seed production ultilizing male sterile line with high sterile plant rate greatly reduced the labor, significantly improved the seed yield, ensuring the seed quality and forming a perfect breeding and seed production system.展开更多
Through the in vitro cell-based assay, a series of experiments to investigate the efficacy of "space ginseng yeast", which were obtained from space breeding technology and modern fermentation engineering wer...Through the in vitro cell-based assay, a series of experiments to investigate the efficacy of "space ginseng yeast", which were obtained from space breeding technology and modern fermentation engineering were conducted in order to provide theoretical basis and scientific guide for the potential applications in cosmetic areas from various aspects. The results have shown that this active ingredient can improve the proliferation, colony forming efficiency and Scratch repair ability of human primary keratinocytes. Moreover, it can significantly increase the expression of Perlecan which is key protein in the basal layer of the skin. Meanwhile, "space ginseng yeast" can help the cell morphology recovery and improve Perlecan, collagen I and elastin expression on the fibroblasts after heavy ion irradiation. All of these results showed that the "space ginseng yeast" displays potential applications on skin regeneration in cosmetic industry.展开更多
基金Supported by "11thFive-Year" Crop Breeding Research of SichuanProvince "11thFive-Year" Joint Breeding Research Project Fun-ding of Sichuan Province.~~
文摘[Objective] The aim was to study the reproduction of the three-line genic male sterile （GMS） lineparent Mian7MB-1 （B. NapusL.） and the seed production of F1 through somatic tissue culture. [Methed] Through hybridization, a new breeding material Mian 7MB-1 in three-line genic temporary maintainer line propagated by tissue culture was used to improve the sterile plant rate of rapeseed in dual-purpose recessive GMS line, such as Mian 7AB type, S45AB type, and etc. And then the variety comparative test was performed. [Result] In order to avoid some fertility restoration phenomena occurring during the process of self-reproduction, Mian 7AB was propagated in bulk with somatic tissue culture of temporary maintainer line plant stem. The propagated temporary maintainer line seedlings were applied to the breeding and seed production of net room male sterile line parent, promoting the sterile plant rate of the male sterile line parent to 91.7% -93.5%. The male sterile line parents per hectare were enough for the seed production of hybrid F1 in 7 500 -15 000 hm^2. [ Conclusion ] Compared with the original dual-purpose GMS line, the seed production ultilizing male sterile line with high sterile plant rate greatly reduced the labor, significantly improved the seed yield, ensuring the seed quality and forming a perfect breeding and seed production system.
文摘Through the in vitro cell-based assay, a series of experiments to investigate the efficacy of "space ginseng yeast", which were obtained from space breeding technology and modern fermentation engineering were conducted in order to provide theoretical basis and scientific guide for the potential applications in cosmetic areas from various aspects. The results have shown that this active ingredient can improve the proliferation, colony forming efficiency and Scratch repair ability of human primary keratinocytes. Moreover, it can significantly increase the expression of Perlecan which is key protein in the basal layer of the skin. Meanwhile, "space ginseng yeast" can help the cell morphology recovery and improve Perlecan, collagen I and elastin expression on the fibroblasts after heavy ion irradiation. All of these results showed that the "space ginseng yeast" displays potential applications on skin regeneration in cosmetic industry.
