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育种攻关协作组织管理的竞争思维与探索
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作者 郝企信 郑彦平 《农业科技管理》 北大核心 1992年第5期29-30,共2页
关键词 科研协作 育种攻关协作组织 竞争机制 农业科研管理
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丹麦的奶牛育种
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作者 董重九 《世界农业》 1987年第4期49-50,共2页
丹麦畜牧业比较发达,以奶牛和猪为主。1983年全国有牛300万头,其中奶牛101万头,主要为黑白花奶牛、丹麦红牛、红白花牛、娟姗牛四大品种。1983年丹麦的奶牛平均每头产奶量5317公斤,居世界第六位。
关键词 黑白花奶牛 产奶量 相对育种 EDP系统 乳脂量 丹麦红牛 育种组织 公牛 娟姗牛 牛的育种
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中国良种黄牛育种委员会二十年工作报告
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《中国牛业科学》 1995年第S1期1-8,共8页
中国良种黄牛育种委员会二十年工作报告(1975—1995)中国良种黄牛育种委员会秘书处各位委员、专家、同志们:今年是中国良种黄牛育种委员会成立二十周年。今天适逢全国著名的养牛专家、博士生导师、中国良种黄牛育种委员会副... 中国良种黄牛育种委员会二十年工作报告(1975—1995)中国良种黄牛育种委员会秘书处各位委员、专家、同志们:今年是中国良种黄牛育种委员会成立二十周年。今天适逢全国著名的养牛专家、博士生导师、中国良种黄牛育种委员会副主任委员兼秘书长邱怀教授八十华诞、... 展开更多
关键词 育种组织 黄牛数量 养牛业 黄牛改良 冷冻精液 河南省 秦川牛 种公牛 发展畜牧业 科学研究
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柑橘品种选育研究进展 被引量:21
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作者 董美超 李进学 +2 位作者 周东果 岳建强 高俊燕 《中国果树》 北大核心 2013年第6期73-78,共6页
综述了柑橘引种、芽变选种、实生选种、杂交育种、组织培养育种、转基因育种和DNA分子标记辅助育种等研究成果;提出了柑橘品种选育中存在的问题,并对柑橘品种选育研究进行了展望。
关键词 柑橘 引种 芽变选种 实生选种 杂交育种 组织培养育种 转基因育种 DNA分子标记
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复州牛选育保种杂交改良工作报告 被引量:1
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作者 宋明琦 高春风 +3 位作者 马广照 高文波 袁淑先 赵峰 《中国牛业科学》 1995年第S1期35-39,共5页
复州牛选育保种杂交改良工作报告宋明琦,高春风,马广照,高文波(辽宁省瓦房店市畜牧技术推广站116300)复州牛是我国一个优良的地方黄牛品种,它产于瓦房店市,在大连市的金州区、普兰店市也有分布.早在200年前.生活在复... 复州牛选育保种杂交改良工作报告宋明琦,高春风,马广照,高文波(辽宁省瓦房店市畜牧技术推广站116300)复州牛是我国一个优良的地方黄牛品种,它产于瓦房店市,在大连市的金州区、普兰店市也有分布.早在200年前.生活在复州河和岚崮河流域的广大农民。为了适... 展开更多
关键词 复州牛 杂交改良 瓦房店市 选育工作 种公牛 育种组织 育种经验 育种工作 母牛 群众性
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Establishment of Three Breeding System of Virus-free Strawberry Plantlet in Infantry Regiment of the Twelve Division 被引量:2
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作者 史芳芳 《Agricultural Science & Technology》 CAS 2014年第7期1076-1078,共3页
Shoot-tip culture of strawberry is the most important way to get virus-free plants.Moreover,detoxification and rapid propagation are the key techniques for producing high-quality seeds and seedlings massively and rapi... Shoot-tip culture of strawberry is the most important way to get virus-free plants.Moreover,detoxification and rapid propagation are the key techniques for producing high-quality seeds and seedlings massively and rapidly and achieving the high-quality,high-yield and high-efficiency development of strawberry industry.We have recently produced virus-free strawberry seedlings by combing tissue culture technology and stolon-reproduction method and this technology has been widely used.In addition,a relatively perfect three breeding system of virus-free strawberry seedlings was established after production practices,guaranteeing the supply of high-quality seeds and seedlings and technical support for strawberry industry in corps. 展开更多
关键词 STRAWBERRY Virus-free seedling Tissue culture Seedling breeding
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Studies of Improving the Frequency of Indica Rice Transformation by Biolistic Bombardment 被引量:1
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作者 易自力 Wang Li +5 位作者 Cao Shouyun Yuan Longping Guan Chunyun Zhau Puhan Tian Wenzhang Chu Chengcai 《High Technology Letters》 EI CAS 2003年第1期6-10,共5页
In order to improve the frequency of indica rice transformation by biolistic bombardment, suitable culture conditions for embryonic calli,an optimal selection scheme for resistant calli and seedlings, and optimum bomb... In order to improve the frequency of indica rice transformation by biolistic bombardment, suitable culture conditions for embryonic calli,an optimal selection scheme for resistant calli and seedlings, and optimum bombardment parameters a investigated by using 14 commercially important indica rice cultivars. The main results show that the CC medium with 36g/L mannitol is a scheme subculture medium in which the browning of indica rice calli can be mitigated significantly; The concentration of 30~40mg/L Hyg or 150~200mg/L G418 or 10~20 mg/L Basta is suitable for selection of resistant calli; The transformation parameters of 100μg gold powder absorbing 0.2μg DNA per shot and 900 psi helium pressure and 6 cm bombardment distance and bombarded twice for each plate give the best result; Keeping the target calli on osmotic medium containing 60g/L mannitol from 12 ~24h before bombardment to 24~48h after it can increase the efficiencies of transformation . Furthermore, some transgenic indica rice plants are obtained using this optimized transformation system. 展开更多
关键词 indica rice biolistic bombardment TRANSFORMATION CALLUS
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MHC polymorphism and disease resistance to Singapore grouper iridovirus(SGIV) in the orange-spotted grouper, Epinephelus coioides 被引量:2
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作者 杨敏 魏京广 +3 位作者 李鹏飞 魏世娜 黄友华 秦启伟 《Science Bulletin》 SCIE EI CAS CSCD 2016年第9期693-699,共7页
Major histocompatibility complex (MHC) genes are critical members in both innate and adaptive immunity, and the association between their polymorphism and disease resistance has been reported in many teleosts. In th... Major histocompatibility complex (MHC) genes are critical members in both innate and adaptive immunity, and the association between their polymorphism and disease resistance has been reported in many teleosts. In the present study, we first investigated the genetic variation at the MHC II β gene in orange-spotted grouper (Epinephelus coioides) after a challenge with Singapore grouper iridovirus (SGIV). The results reveal that a high polymorphism level of the MHC II β gene (H = 1.000; K = 20.206; π=0.081) and at least three loci exist in grouper. The rate of dN/dS in the peptide-binding region (PBR) and non-PBR were both 〉1, suggesting the loci were evolving under positive selection. A high ratio of heterozygous individuals (37.26 %) and high rate of dN/dS were discovered, suggesting that both heterozygote advantage and frequency-dependent selection might result in the high polymorphism levels in MHC II β genes in grouper. A total of 33 MHC II β alleles were identified from 40 high-susceptibility (HS) and 40 high-re- sistance group (HR) individuals, and 15 alleles were used in the association analysis. Three alleles, EPCO-DBB*0302, EPCO-DBB*0307, EPCO-DBB*0603, and EPCO- DBB*1001 were significantly associated with resistance ability to SG1V, and the EPCO-DBB*0607 and EPCO-DBB*1303 alleles were associated with susceptibility (P 〈 0.05). To further confirm the association, another independent challenge experiment was performed. The result of association analysis in the verification test found that only EPCO-DBB*1001 alleles were significantly asso- ciated with resistance to SGIV (P 〈 0.05), while the other alleles showed no significance (P 〉 0.05) in the frequency distribution between HR and HS groups. Therefore, the EPCO-DBB* 1001 alleles could be used as a disease resis- tance-related MHC marker in the molecular marker-assisted selective breeding program of grouper. 展开更多
关键词 Orange-spotted grouper Majorhistocompatibility complex II β Singapore grouperiridovirus Disease resistance
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