Genus Nassarius contains many subgenera, such as Zeuxis, Telasco, Niotha, Varicinassa, Plicarcularia, Nassarius s. str. and Reticunassa. On the basis of morphological characteristics of the shell and radula and sequen...Genus Nassarius contains many subgenera, such as Zeuxis, Telasco, Niotha, Varicinassa, Plicarcularia, Nassarius s. str. and Reticunassa. On the basis of morphological characteristics of the shell and radula and sequences of mitochondrial cytochrome oxidase subunit I (COI) and 16S rRNA genes, Nassarius specimens collected from the South China Sea were identified and phylogenetically analyzed. Although Nassarius sp. and Nassarius (Varicinassa) variciferus were morphologically different in their shells, few variations were found among their radular teeth and sequences of mtCOI and mt16S RNA genes. Therefore, Nassarius sp. should be classified as N. (Varicinassa) variciferus. Nassarius (Zeuxis) sp. has only a subtle difference from Nassarius (Zeuxis) algidus on the shell, but it shows obvious differences in radular teeth and DNA sequence, indicating that they are two distinct species. Sequence divergence of mtCOI and mt16S RNA genes within Nassarius species was much lower than that between species, suggesting that these two genes are suitable for Nassarius species identification. Phylogenetic analysis (neighbor-joining and maximum parsimony) based on mtCOI and mt16S rRNA genes revealed the presence of two groups in genus Nassarius and a closest relationship between subgenera Zeuxis and Telasco. Species of subgenus Plicarcularia did not form a single clade. The molecular phylogeny was not congruent with the previous morphological phylogeny. The subgeneric divisions of genus Nassarius appear to be uncertain and unreliable.展开更多
The present study was conducted to identify K-casein （Kappa-casein） polymorphism in the Bali cattle （Bos sondaicus）. A 780 bp specific fragment of K-casein gene spanning from the forth exon region （517 bp） to fo...The present study was conducted to identify K-casein （Kappa-casein） polymorphism in the Bali cattle （Bos sondaicus）. A 780 bp specific fragment of K-casein gene spanning from the forth exon region （517 bp） to forth intron （263 bp） was amplified from 60 individuals from the Bali Cattle Breeding Centre in Jembrana regency, Bali province. PCR-RFLP analysis with HindlII indicated that only the BB genotype was found at locus I, while with PstI indicated that two genotypes AA and AB were found at the k-casein locus If. The frequencies of A and B alleles at locus II were 0.91 and 0.09, respectively. Mutations in locus II did not change the amino acid coding （silent mutation）. At locus I, comparison with allele frequencies in other cattle breeds indicates that frequencies in Bali cattle （Bos sondaicus） are significantly different from those observed in Bos taurus and Bos indicus breeds.展开更多
Tea plant is cross pollinated plant, it forms a variety of the tea Germplasm constantly in the long-term breeding process. Tea Germplasm resources are the material foundation for improving varieties and also the basic...Tea plant is cross pollinated plant, it forms a variety of the tea Germplasm constantly in the long-term breeding process. Tea Germplasm resources are the material foundation for improving varieties and also the basic research which is good for developing and expanding tea well-bred varieties in tea field, especially in quality selection and biotechnology research. Furthermore, excellent tea germplasm is primary condition for tea breeding and quality. Therefore, identification and screening excellent tea germplasm become particularly important. We need accelerate develop high-quality tea varieties, and it need to base on exploring and studying tea germplasm. This paper reviews the research progress of tea germplasm on identification from morphological characteristic, biological characteristic, quality characteristic, resistance and heredity. However, there are still some problems on identification and screening of tea germplasm, no effective methods have been found so far which apply on early identification and genetic engineering, so we should make intensive and long lasting study tea germplasm and analyze systematically various identification, which are benefit for breeding high-quality seeds, shortening the breeding period and summing the new way of breeding. We expect them to play the key roles and provide the theory basis for genetic improvement, classification and breeding in tea plant.展开更多
Mitochondrial genome has been widely used in species identification and gene conservation.In the present study,the complete mitochondrial genome of Oula sheep(Ovis aries)was determined using next-generation sequencing...Mitochondrial genome has been widely used in species identification and gene conservation.In the present study,the complete mitochondrial genome of Oula sheep(Ovis aries)was determined using next-generation sequencing.This genome was16 618 bp(NCBI accession number:KU575248)and contained 13 protein coding genes,22 transfer RNA genes,two ribosomal RNA genes,and a typical control region.The overall nucleotide composition was 33.7%A,27.4%T,25.8%C,and 13.1%G,with a total A+T content of 61.1%.The phylogenetic analysis of selected sheep breeds showed that Oula sheep were clustered within branch A and originated from approximately 6 ka.This mitochondrial genome will provide valuable information for molecular genetic research of Oula sheep.展开更多
基金Supported by the Youth Science Foundation of the State Oceanic Administration (No. 2009106)the Directorate Foundation of South China Sea Branch, the State Oceanic Administration (No. 0815)
文摘Genus Nassarius contains many subgenera, such as Zeuxis, Telasco, Niotha, Varicinassa, Plicarcularia, Nassarius s. str. and Reticunassa. On the basis of morphological characteristics of the shell and radula and sequences of mitochondrial cytochrome oxidase subunit I (COI) and 16S rRNA genes, Nassarius specimens collected from the South China Sea were identified and phylogenetically analyzed. Although Nassarius sp. and Nassarius (Varicinassa) variciferus were morphologically different in their shells, few variations were found among their radular teeth and sequences of mtCOI and mt16S RNA genes. Therefore, Nassarius sp. should be classified as N. (Varicinassa) variciferus. Nassarius (Zeuxis) sp. has only a subtle difference from Nassarius (Zeuxis) algidus on the shell, but it shows obvious differences in radular teeth and DNA sequence, indicating that they are two distinct species. Sequence divergence of mtCOI and mt16S RNA genes within Nassarius species was much lower than that between species, suggesting that these two genes are suitable for Nassarius species identification. Phylogenetic analysis (neighbor-joining and maximum parsimony) based on mtCOI and mt16S rRNA genes revealed the presence of two groups in genus Nassarius and a closest relationship between subgenera Zeuxis and Telasco. Species of subgenus Plicarcularia did not form a single clade. The molecular phylogeny was not congruent with the previous morphological phylogeny. The subgeneric divisions of genus Nassarius appear to be uncertain and unreliable.
文摘The present study was conducted to identify K-casein （Kappa-casein） polymorphism in the Bali cattle （Bos sondaicus）. A 780 bp specific fragment of K-casein gene spanning from the forth exon region （517 bp） to forth intron （263 bp） was amplified from 60 individuals from the Bali Cattle Breeding Centre in Jembrana regency, Bali province. PCR-RFLP analysis with HindlII indicated that only the BB genotype was found at locus I, while with PstI indicated that two genotypes AA and AB were found at the k-casein locus If. The frequencies of A and B alleles at locus II were 0.91 and 0.09, respectively. Mutations in locus II did not change the amino acid coding （silent mutation）. At locus I, comparison with allele frequencies in other cattle breeds indicates that frequencies in Bali cattle （Bos sondaicus） are significantly different from those observed in Bos taurus and Bos indicus breeds.
文摘Tea plant is cross pollinated plant, it forms a variety of the tea Germplasm constantly in the long-term breeding process. Tea Germplasm resources are the material foundation for improving varieties and also the basic research which is good for developing and expanding tea well-bred varieties in tea field, especially in quality selection and biotechnology research. Furthermore, excellent tea germplasm is primary condition for tea breeding and quality. Therefore, identification and screening excellent tea germplasm become particularly important. We need accelerate develop high-quality tea varieties, and it need to base on exploring and studying tea germplasm. This paper reviews the research progress of tea germplasm on identification from morphological characteristic, biological characteristic, quality characteristic, resistance and heredity. However, there are still some problems on identification and screening of tea germplasm, no effective methods have been found so far which apply on early identification and genetic engineering, so we should make intensive and long lasting study tea germplasm and analyze systematically various identification, which are benefit for breeding high-quality seeds, shortening the breeding period and summing the new way of breeding. We expect them to play the key roles and provide the theory basis for genetic improvement, classification and breeding in tea plant.
基金Supported by the Central Public-Interest Scientific Institution Basal Research Fund(1610322016006)Science and Technology Support Projects in Gansu Province(1504NKCA052)the Innovation Project of Chinese Academy of Agricultural Sciences(CAAS-ASTIP-2014-LIHPS-01)
文摘Mitochondrial genome has been widely used in species identification and gene conservation.In the present study,the complete mitochondrial genome of Oula sheep(Ovis aries)was determined using next-generation sequencing.This genome was16 618 bp(NCBI accession number:KU575248)and contained 13 protein coding genes,22 transfer RNA genes,two ribosomal RNA genes,and a typical control region.The overall nucleotide composition was 33.7%A,27.4%T,25.8%C,and 13.1%G,with a total A+T content of 61.1%.The phylogenetic analysis of selected sheep breeds showed that Oula sheep were clustered within branch A and originated from approximately 6 ka.This mitochondrial genome will provide valuable information for molecular genetic research of Oula sheep.
