[Objective] The aim was to study the techniques for the tissue culture,rapid propagation and transplantation of Anthurium andraeanum.[Method] The leaf,leaf stalk,spathe and spadix of the pot flower variety and cut flo...[Objective] The aim was to study the techniques for the tissue culture,rapid propagation and transplantation of Anthurium andraeanum.[Method] The leaf,leaf stalk,spathe and spadix of the pot flower variety and cut flower variety of A.andraeanum were used as explants,in order to investigate the influences of the explants selection,disinfection conditions and different media on the callus induction,proliferation and rooting.[Result] The leaf stalk of A.andraeanum was the optimum explant,with the highest callus induction rate（78.3%）.The best disinfecting effect could be obtained when the explants was sterilized with 0.1% HgCl2 for 8 min.The optimum media for the callus induction of Ping Champion and MIDORI were respectively 1/2 MS＋1.50 mg/L 6-BA＋0.50 mg/L 2,4-D＋0.10 mg/L NAA and 1/2 MS＋1.50 mg/L 6-BA＋1.00 mg/L KT＋0.10 mg/L 2,4-D,while the optimum media for callus subculture of Ping Champion and MIDORI were respectively MS＋1.00 mg/L 6-BA＋0.50 mg/L NAA＋0.30 mg/L 2,4-D and MS＋0.50 mg/L 6-BA＋0.50 mg/L KT＋0.10 mg/L NAA.It was beneficial for the proliferation and growth of cluster buds while 10%（w/v）of coconut water or banana jam was added to MS medium at the proliferation stage,and the proliferation rate could be increased by 2.83 fold.At the rooting stage,the medium 1/2 MS＋0.10 mg/L NAA＋0.10 mg/L IAA was the optimum medium for rooting,and the rooting rate was up to 100%.[Conservation] The research results will lay a foundation for the establishment of the industrial seedling breeding system of A.andraeanum,and it will be significant for the development of A.andraeanum industry.展开更多
Shoot-tip culture of strawberry is the most important way to get virus-free plants.Moreover,detoxification and rapid propagation are the key techniques for producing high-quality seeds and seedlings massively and rapi...Shoot-tip culture of strawberry is the most important way to get virus-free plants.Moreover,detoxification and rapid propagation are the key techniques for producing high-quality seeds and seedlings massively and rapidly and achieving the high-quality,high-yield and high-efficiency development of strawberry industry.We have recently produced virus-free strawberry seedlings by combing tissue culture technology and stolon-reproduction method and this technology has been widely used.In addition,a relatively perfect three breeding system of virus-free strawberry seedlings was established after production practices,guaranteeing the supply of high-quality seeds and seedlings and technical support for strawberry industry in corps.展开更多
基金Supported by National Science and Technology Supporting Project(2007BAD45B07)Key Technology Research and Development Project of Hainan Province(080201,2009YFZX003)~~
文摘[Objective] The aim was to study the techniques for the tissue culture,rapid propagation and transplantation of Anthurium andraeanum.[Method] The leaf,leaf stalk,spathe and spadix of the pot flower variety and cut flower variety of A.andraeanum were used as explants,in order to investigate the influences of the explants selection,disinfection conditions and different media on the callus induction,proliferation and rooting.[Result] The leaf stalk of A.andraeanum was the optimum explant,with the highest callus induction rate（78.3%）.The best disinfecting effect could be obtained when the explants was sterilized with 0.1% HgCl2 for 8 min.The optimum media for the callus induction of Ping Champion and MIDORI were respectively 1/2 MS＋1.50 mg/L 6-BA＋0.50 mg/L 2,4-D＋0.10 mg/L NAA and 1/2 MS＋1.50 mg/L 6-BA＋1.00 mg/L KT＋0.10 mg/L 2,4-D,while the optimum media for callus subculture of Ping Champion and MIDORI were respectively MS＋1.00 mg/L 6-BA＋0.50 mg/L NAA＋0.30 mg/L 2,4-D and MS＋0.50 mg/L 6-BA＋0.50 mg/L KT＋0.10 mg/L NAA.It was beneficial for the proliferation and growth of cluster buds while 10%（w/v）of coconut water or banana jam was added to MS medium at the proliferation stage,and the proliferation rate could be increased by 2.83 fold.At the rooting stage,the medium 1/2 MS＋0.10 mg/L NAA＋0.10 mg/L IAA was the optimum medium for rooting,and the rooting rate was up to 100%.[Conservation] The research results will lay a foundation for the establishment of the industrial seedling breeding system of A.andraeanum,and it will be significant for the development of A.andraeanum industry.
文摘Shoot-tip culture of strawberry is the most important way to get virus-free plants.Moreover,detoxification and rapid propagation are the key techniques for producing high-quality seeds and seedlings massively and rapidly and achieving the high-quality,high-yield and high-efficiency development of strawberry industry.We have recently produced virus-free strawberry seedlings by combing tissue culture technology and stolon-reproduction method and this technology has been widely used.In addition,a relatively perfect three breeding system of virus-free strawberry seedlings was established after production practices,guaranteeing the supply of high-quality seeds and seedlings and technical support for strawberry industry in corps.
