[Objective] The aim was to study the supporting raising technology of Castanea mollissima Blume molecular marker-assisted selection to reduce the cost and time for chestnut breeding.[Method] To find the best cutting m...[Objective] The aim was to study the supporting raising technology of Castanea mollissima Blume molecular marker-assisted selection to reduce the cost and time for chestnut breeding.[Method] To find the best cutting method,10 treatments were designed according to transverse diameter and vertical diameter of the chestnut including different cutting ratios in 1/2,1/3,1/4 and different directions about cross cutting,vertical cutting,bevel cutting and two side-cutting.The germination rate,seedling survival rate and morphological indicators of the treatments were measured;significant difference among them was also analyzed.[Result] 1/3 bevel cutting was the best,which not only could give high germination rate and survival rate,but also could guarantee high quality of nursery stock,which did not grow so differently with the untreated seedlings.[Conclusion] This way can not only reduce too much cost and time for seedling breeding,but also promote the germination of chestnut in advance,which could do much good to production.展开更多
[Objective] This study aimed to solve the problems of low seed germina- tion rate and difficult bareroot seedling transplanting in artificial cultivation of Arto- carpus nanchuanensis, a native species in China. [Meth...[Objective] This study aimed to solve the problems of low seed germina- tion rate and difficult bareroot seedling transplanting in artificial cultivation of Arto- carpus nanchuanensis, a native species in China. [Method] Under different seed treatment and seedling style, the seed germination rate and seedling emergence rate of A. nanchuanensis were investigated. [Result] tn both sand bed and direct sowing yellow soil, the seed germination rate, seedling survival rate and seedling emergence rate in the treatment II were all higher than those in the treatments I and III. The average seed germination rate, seedling survival rate and seedling emergence rate in sand beds were 1.5%, 16.27% and 13.95%, respectively higher than those in direct sowing yellow soil. The average seedling emergence rate in sand beds was 2.46% higher than that in containers. Among the 8 kinds of light matrix formulas, the growth of seedlings in the formula VIII was best. In the three treatment groups, the seedling emergence rates, seedling heights and ground diame- ters in the formula VIII were all significantly different from those in the other formu- las. The seedling growth in the formula I and III ranked second, while the seedling emergence rate, seedling height and ground diameter in the formula VII were the poorest. [Conclusion] If conditions allow, the formulas I (meadow soil:vermiculite:yel- low soi1=5:3:2), III (meadow soil:perlite:yellow soi1=5:3:2) and VIII (peat:forest top soi1=6:4) should be promoted in seedling with light matrixes.展开更多
Using foxtail millet (Setaria italica (L.) Beauv.) male-sterile line 1066A as female parent and Yugu 1 primary trisomic series (1 - 7) and tetrasomics 8, 9 as male parents, chromosome location of gene for male-sterili...Using foxtail millet (Setaria italica (L.) Beauv.) male-sterile line 1066A as female parent and Yugu 1 primary trisomic series (1 - 7) and tetrasomics 8, 9 as male parents, chromosome location of gene for male-sterility and yellow seedling in line 1066A was studied by primary trisomic analysis. The plants of F-1 generation of trisomics 2 - 9 were obtained by crossing with a great many plants of 1066A. F-1 generation of trisomics was similar to their male parent in morphologic characters, the color of their seedling was green, and pollen was partially fertile. The segregation ratio of fertility to sterility is 3:1 in F-2 generation of trisomics 2, 3, 4, 5, 7, 8 and 9; and 14:1 only in F-2 generation of trisomic 6 (chi(0.05)(2) = 0.012). The segregation ratio of green seedling to yellow seedling is 12:1 only in F-2 generation of trisomic 7 (chi(0.05)(2) = 0.31), but in other cases, this ratio is 3:1. The results indicated that the male-sterility gene was located on chromosome 6, and the gene for yellow seedling was monogenic recessive and located on chromosome 7. The rate of trisomics transmission by pollen was tested, trisomics 8 and 9 were the highest in rates of trisomics transmission and followed by trisomics 6 and 4.展开更多
This study was conducted to investigate the effects of bast fiber seedling film on rice seedling growth. In this study, indica cultivar 'Xiangwanxian 13' and indica hybrid cuitivar 'Y Liangyou 1' were used to inve...This study was conducted to investigate the effects of bast fiber seedling film on rice seedling growth. In this study, indica cultivar 'Xiangwanxian 13' and indica hybrid cuitivar 'Y Liangyou 1' were used to investigate the changes of aboveground part growth and root respiration enzyme activities of rice seedlings af- ter the application of bast fiber seedling film. The results showed that, compared with the CK, the accumulation of shoot biomass, chlorophyll content, root bleeding intensity, soluble sugar content, and nitrate nitrogen content were significantly im- proved with the application of bast fiber seedling film. In addition, the activities of malate dehydrogenas (MDH) and alcohol dehydrogenas (ADH) were higher in rice seedling roots raised with bast fiber seedling film, while there was no significant change in lactate dehydrogenase (LDH) after the application of bast fiber seedling film. However, there were no significant differences in these indexes between the two cultivars. All the results indicated that the growth and development of rice seedlings could be improved with the application of bast fiber seedling film.展开更多
基金Supported by Key Projects in the National Science&Technology Pil-lar Program during the Eleventh Five-Year Plan Period(2006BAD01A1703-1)~~
文摘[Objective] The aim was to study the supporting raising technology of Castanea mollissima Blume molecular marker-assisted selection to reduce the cost and time for chestnut breeding.[Method] To find the best cutting method,10 treatments were designed according to transverse diameter and vertical diameter of the chestnut including different cutting ratios in 1/2,1/3,1/4 and different directions about cross cutting,vertical cutting,bevel cutting and two side-cutting.The germination rate,seedling survival rate and morphological indicators of the treatments were measured;significant difference among them was also analyzed.[Result] 1/3 bevel cutting was the best,which not only could give high germination rate and survival rate,but also could guarantee high quality of nursery stock,which did not grow so differently with the untreated seedlings.[Conclusion] This way can not only reduce too much cost and time for seedling breeding,but also promote the germination of chestnut in advance,which could do much good to production.
基金Supported by Wildlife Conservation and Nature Reserve Construction Projects of Chongqing City in 2013~~
文摘[Objective] This study aimed to solve the problems of low seed germina- tion rate and difficult bareroot seedling transplanting in artificial cultivation of Arto- carpus nanchuanensis, a native species in China. [Method] Under different seed treatment and seedling style, the seed germination rate and seedling emergence rate of A. nanchuanensis were investigated. [Result] tn both sand bed and direct sowing yellow soil, the seed germination rate, seedling survival rate and seedling emergence rate in the treatment II were all higher than those in the treatments I and III. The average seed germination rate, seedling survival rate and seedling emergence rate in sand beds were 1.5%, 16.27% and 13.95%, respectively higher than those in direct sowing yellow soil. The average seedling emergence rate in sand beds was 2.46% higher than that in containers. Among the 8 kinds of light matrix formulas, the growth of seedlings in the formula VIII was best. In the three treatment groups, the seedling emergence rates, seedling heights and ground diame- ters in the formula VIII were all significantly different from those in the other formu- las. The seedling growth in the formula I and III ranked second, while the seedling emergence rate, seedling height and ground diameter in the formula VII were the poorest. [Conclusion] If conditions allow, the formulas I (meadow soil:vermiculite:yel- low soi1=5:3:2), III (meadow soil:perlite:yellow soi1=5:3:2) and VIII (peat:forest top soi1=6:4) should be promoted in seedling with light matrixes.
文摘Using foxtail millet (Setaria italica (L.) Beauv.) male-sterile line 1066A as female parent and Yugu 1 primary trisomic series (1 - 7) and tetrasomics 8, 9 as male parents, chromosome location of gene for male-sterility and yellow seedling in line 1066A was studied by primary trisomic analysis. The plants of F-1 generation of trisomics 2 - 9 were obtained by crossing with a great many plants of 1066A. F-1 generation of trisomics was similar to their male parent in morphologic characters, the color of their seedling was green, and pollen was partially fertile. The segregation ratio of fertility to sterility is 3:1 in F-2 generation of trisomics 2, 3, 4, 5, 7, 8 and 9; and 14:1 only in F-2 generation of trisomic 6 (chi(0.05)(2) = 0.012). The segregation ratio of green seedling to yellow seedling is 12:1 only in F-2 generation of trisomic 7 (chi(0.05)(2) = 0.31), but in other cases, this ratio is 3:1. The results indicated that the male-sterility gene was located on chromosome 6, and the gene for yellow seedling was monogenic recessive and located on chromosome 7. The rate of trisomics transmission by pollen was tested, trisomics 8 and 9 were the highest in rates of trisomics transmission and followed by trisomics 6 and 4.
基金Supported by Scientific and Technological Innovation Project of Chinese Academy of Agricultural Sciences(ASTIP-IBFC07)Production Post of Degradable Bast Fiber Mulching Film of National Agro-Industry Technology Research System for Bast and Leaf Fiber Crops(CARS-19-E23)~~
文摘This study was conducted to investigate the effects of bast fiber seedling film on rice seedling growth. In this study, indica cultivar 'Xiangwanxian 13' and indica hybrid cuitivar 'Y Liangyou 1' were used to investigate the changes of aboveground part growth and root respiration enzyme activities of rice seedlings af- ter the application of bast fiber seedling film. The results showed that, compared with the CK, the accumulation of shoot biomass, chlorophyll content, root bleeding intensity, soluble sugar content, and nitrate nitrogen content were significantly im- proved with the application of bast fiber seedling film. In addition, the activities of malate dehydrogenas (MDH) and alcohol dehydrogenas (ADH) were higher in rice seedling roots raised with bast fiber seedling film, while there was no significant change in lactate dehydrogenase (LDH) after the application of bast fiber seedling film. However, there were no significant differences in these indexes between the two cultivars. All the results indicated that the growth and development of rice seedlings could be improved with the application of bast fiber seedling film.