[Objective] The aim was to study the effects of short-term high temperature stress on the photosynthesis of potato in different growth stages. [Method] Choosing powder potato named Longshu No.3 widely cultivated in Ni...[Objective] The aim was to study the effects of short-term high temperature stress on the photosynthesis of potato in different growth stages. [Method] Choosing powder potato named Longshu No.3 widely cultivated in Ningxia as test material,the changes of stomata conductance (Gs),transpiration rate (Tr) and CO2 concentration difference between internal and external leaf chamber,net photosynthetic rate (Pn) and photosynthetic water use efficiency (WUE) in different growth stages under short-term high temperature were analyzed. [Result] During seedling stage,the hysteretic nature of net photosynthetic rate and CO2 concentration difference between internal and external leaf chamber of potato could be found under high temperature stress,while the change trends of stomata conductance and transpiration rate under high temperature stress were consistent to that at normal temperature,but stomata conductance and transpiration rate were higher than those at normal temperature,and CO2 concentration difference between internal and external leaf chamber affected net photosynthetic rate most obviously. During branching stage,the change trends of net photosynthetic rate,CO2 concentration difference between internal and external leaf chamber,stomata conductance and transpiration rate under high temperature stress and normal temperature were similar,but they changed abruptly and reached peak value at noon under high temperature stress,while there existed consistent variation of water use efficiency under high temperature stress and at normal temperature,and CO2 concentration difference between internal and external leaf chamber also affected net photosynthetic rate most greatly,next came transpiration rate. [Conclusion] High temperature stress affected the photosynthesis of potato in different growth stages,and it was more obvious during branching stage than seedling stage,while CO2 concentration difference between internal and external leaf chamber had the most important influence on net photosynthetic rate.展开更多
The Small ubiquitin-related modifier (SUMO) conjugation to a variety of proteins regulates diverse cellular processes, including transcription, cell cycle regulation and maintenance of genome integrity. To investiga...The Small ubiquitin-related modifier (SUMO) conjugation to a variety of proteins regulates diverse cellular processes, including transcription, cell cycle regulation and maintenance of genome integrity. To investigate in vivo biological function of SUMO paralogs, we inactivated them in the early development of zebrafish. While zebrafish embryos deficient for all three SUMO paralogs, as Ubc9-deficient ones, displayed severe defects, loss of individual SUMO paralog was compatible with a normal development. SUMO-deficient embryos can be rescued by a single human or zebrafish SUMO. While key structural basic lysine residues and N-terminal unstructured stretch of SUMO are critical for in vivo rescue, the consensus Kll sumoylation site of SUMO2 is dispensable, implying that chain formation on this potential site is unessential for normal development. Inactivation of all three SUMOs triggered p53- dependent apoptosis and further inactivation of p53 restored normal zebrafish development. Interestingly, we also demonstrate that the dominant negative truncated form of p53, Δ113p53, significantly blunts SUMO depletion-induced p53 activity in vivo. Taken together, our results suggest that SUMO paralogs are indispensable, but redundant, in the early development of zebrafish.展开更多
This study investigated the embryonic development of Maldives clownfish Amphiprion nigripes under natural conditions (28 - 30℃) at a lagoon of Agatti Island, Lakshadweep, India. The newly deposited fish egg was capsu...This study investigated the embryonic development of Maldives clownfish Amphiprion nigripes under natural conditions (28 - 30℃) at a lagoon of Agatti Island, Lakshadweep, India. The newly deposited fish egg was capsule-shaped and orange-red, with a (0.73 ± 0.04) mm3 yolk containing 5 - 10 fat globules. The embryonic development of fertilized eggs was divided into 26 stages and the time elapsing for each stage was recorded. Results showed that the cleavage was rapid, with the first division observed 1 h 20 min after fertilization. Blastulation occurred 4 h later, followed by gastrulation 12 h after fertilization, with a yolk volume of (0.61 ± 0.06) mm3. The organogenesis process started 22 h after fertilization when the blastopores closed and notochord formation began. The embryonic stage was recorded 24 h later, with the appearance of forebrain, midbrain, hindbrain, melanophores on yolk-sac and 22 somites, and a decreased yolk volume of (0.54 ± 0.08) mm3. Other organs developed well 31 h after fertilization, whereas the heart started beating and blood circulation began 78 h later. Red pigmentation (erytrophores) appeared 96 h after fertilization, with a small yolk volume of (0.22 ± 0.02) mm3. Mouth developed well and eyes were noticeable 120 h later, with head, pectoral fin and tail frequently moving 144 h after fertilization. The embryo reached the pre-hatching stage 168 h later and started to hatch after 170 - 180 h incubation. This study first detailed the embryonic development and yolk absorption of A. nigripes under natural conditions.展开更多
[Objective] This study was to investigate the effects of fulvic acid on agronomic traits and yield of autumn potato. [Method] The effects of fulvic acid on growth period, plant morphology, tuber, net photosynthetic ra...[Objective] This study was to investigate the effects of fulvic acid on agronomic traits and yield of autumn potato. [Method] The effects of fulvic acid on growth period, plant morphology, tuber, net photosynthetic rate, chlorophyll content, leaf area index (LAI) and yield of autumn potato were investigated. [Result] The application of fulvic acid prolonged the growth period and increased the plant height, stem diameter, leaf chlorophyll content, LAI, net photosynthetic rate, tuber number per plant, tuber weight per plant and marketable tuber rate of autumn potato, thus increasing the yield. Applying fulvic acid prolonged the growth period of autumn potato by 1-3 d and significantly increased the plant height, net photosynthetic rate, tuber weight per plant and yield of autumn potato (P〈0.05, P〈0.01). When the application amount of compound fertilizer was reduced by half, applying fulvic acid almost showed the same seedling emergence stage, mature stage, growth period and net photosynthetic rate with applying full amount of compound fertilizer. In particular, no significant difference was found in the yield of autumn potato between fullamount and half-amount compound fertilizer treatment groups in the premise of solid- liquid fulvic acid combined application. [Conclusion] In the premise of unchanged yield, applying fulvic acid can reduce the application of chemical fertilizers and reduce the non-point source pollution of chemical fertilizers.展开更多
Cyclin-dependent kinase 7 (Cdk7) is the catalytic subunit of the metazoan Cdk-activating kinase (CAK). Activation of Cdk7 requires its association with a regulatory subunit, Cyclin H. Although the Cdk7/Cyclin H co...Cyclin-dependent kinase 7 (Cdk7) is the catalytic subunit of the metazoan Cdk-activating kinase (CAK). Activation of Cdk7 requires its association with a regulatory subunit, Cyclin H. Although the Cdk7/Cyclin H complex has been implicated in the regulation ofRNA polymerase in several species, the precise function of their orthologs in zebrafish has not been fully elucidated. In this study, we isolated from zebrafish blastula embryos two cDNAs encoding the orthologs of human Cyclin H and Cdk7, and examined the role of Cdk7/Cyclin H in zebrafish embryogenesis. Sequence analysis showed that the zebrafish Cyclin H and Cdk7 cDNAs encode proteins with 65% and 86% identity to the respective hu- man orthologs. RT-PCR and whole-mount in situ hybridization analyses of their expression in unfertilized eggs, embryos and organs of adult fish suggested that Cyclin H and Cdk7 messages are maternally loaded. Our data also showed that their transcripts were detected throughout development. Distribution of Cyclin H transcripts was found to be ubiquitous during early stages of development and become restricted to the anterior neural tube, brain, eyes, procreate tissues, liver and heart by 5 days post-fertilization. Expression of a dominant-negative form of Cyclin H delayed the onset of zygotic transcription in the early embryo, resulting in apoptosis at 5 hours post-fertilization and leading to sever defects in tis- sues normally exhibiting high levels of Cyclin H expression. These results implicate Cyclin H in the regulation of the transcriptional machinery during midblastula transition and suggest that it is an essential gene in early zebrafish larval development.展开更多
In its effort towards becoming a developed nation in the next decade, Malaysia has incorporated its education system with National Higher Education Action Plan initiatives to produce a next generation of populace who ...In its effort towards becoming a developed nation in the next decade, Malaysia has incorporated its education system with National Higher Education Action Plan initiatives to produce a next generation of populace who are no more dependent on the government for various aids. For the past decade, creation of entrepreneurs through a formal education has become the mainstay of the local Higher Education Institutions (HEI). Pursuing of an entrepreneurship education by means of a degree programme has emerged as one of the popular choice among undergraduates as an option for rewarding and full time career. Because of this. there has been an increased demand for entrepreneurship education and training in Malaysia to nurture entrepreneurial characteristics. The national agenda is to encourage the younger generations to be nurtured into becoming job-creators rather than job-seekers once they leave the educational system. Given this trend, HEIs need to undertake some policy changes, including faculty or school overall legislation and the development of alternative specialized programs, to be more accommodating to future entrepreneurs. This paper provides an insight into entrepreneurship education, as one of the programs that can generate interest among undergraduates to enhance entrepreneurial characteristics in order to create good entrepreneurs.展开更多
The objectives of this study arc: first, to obtain a description that a political party website is an effective means of communication to carry out socialization programs to members ofthc party or the party's virtua...The objectives of this study arc: first, to obtain a description that a political party website is an effective means of communication to carry out socialization programs to members ofthc party or the party's virtual public; second, to obtain a description that websitc is a useful means to give political education about people's political rights; third, to explore the implication of web's content for strengthening the political party's identity. The research approach used was interpretive approach to qualitative-descriptive methods. Interpretive method emphasizes culture from the perspective of social construction theory (Papa, Danicls, & Spiker, 2008). Data collection (Mann & Stewart, 2000) conducted through site analysis of the People Justice's Party (Parti Kcadilan Rakyat) Malaysia website from January 16 to March 31 2010. The findings of this study arc the website is an effective means to disseminate political party's program. Although, the virtual public or party members were preferring a direct face-to-face interpersonal communication. The People Justice's Party, Malaysia has 14 MPN (Majclis Pimpinan Negeri/Rcgional Board) in the state-level. Regarding the political atmosphere in Malaysia, the wcbsite was an efficient tool. The costs required were very cheap. Second, the presence of party website opened a space for providing political education to more people. Third, the public responses stimulated improvement and strengthening the political party's identity.展开更多
Teachers of CLD (culturally and linguistically diverse) public school students New York and Rome, Italy were interviewed about their work with this unique, growing subset of learners. The 12 teachers' stories highl...Teachers of CLD (culturally and linguistically diverse) public school students New York and Rome, Italy were interviewed about their work with this unique, growing subset of learners. The 12 teachers' stories highlight myriad issues facing educators across the globe. Each teacher had recently participated in a research-based and government-sponsored professional development program focused on second language acquisition and culturally responsive pedagogy. Themes emerged through the analysis of individual interviews and classroom observations. These findings highlight teachers' perceptions of effective professional development, along with identification of institutional barriers impeding the delivery of best practices to CLD students. Cross-cultural comparisons are made which suggest an inconsistency in educational services for CLD students, along with an urgent need for professional development courses for teachers and administrators. The educators' voices shed light on both the challenges and rewards associated with this important work.展开更多
基金Supported by National Natural Science Foundation of China(40765003)National Key Technology R &D Program in the 11th Five Year Plan of China (2007BAC03A02)~~
文摘[Objective] The aim was to study the effects of short-term high temperature stress on the photosynthesis of potato in different growth stages. [Method] Choosing powder potato named Longshu No.3 widely cultivated in Ningxia as test material,the changes of stomata conductance (Gs),transpiration rate (Tr) and CO2 concentration difference between internal and external leaf chamber,net photosynthetic rate (Pn) and photosynthetic water use efficiency (WUE) in different growth stages under short-term high temperature were analyzed. [Result] During seedling stage,the hysteretic nature of net photosynthetic rate and CO2 concentration difference between internal and external leaf chamber of potato could be found under high temperature stress,while the change trends of stomata conductance and transpiration rate under high temperature stress were consistent to that at normal temperature,but stomata conductance and transpiration rate were higher than those at normal temperature,and CO2 concentration difference between internal and external leaf chamber affected net photosynthetic rate most obviously. During branching stage,the change trends of net photosynthetic rate,CO2 concentration difference between internal and external leaf chamber,stomata conductance and transpiration rate under high temperature stress and normal temperature were similar,but they changed abruptly and reached peak value at noon under high temperature stress,while there existed consistent variation of water use efficiency under high temperature stress and at normal temperature,and CO2 concentration difference between internal and external leaf chamber also affected net photosynthetic rate most greatly,next came transpiration rate. [Conclusion] High temperature stress affected the photosynthesis of potato in different growth stages,and it was more obvious during branching stage than seedling stage,while CO2 concentration difference between internal and external leaf chamber had the most important influence on net photosynthetic rate.
基金Supplementary information is linked to the online version of the paper on the Cell Research website.Acknowledgements We thank Dr Jiang Zhu (Shanghai institute of hematology, Rui Jin hospital) and Dr Nelly Kieffer (CNRS LIA, Rui Jin hospital) for their comments. This work was supported by grants from the National High Tech Program of China (863, 2006AA02Z150), the National Science Foundation of China (30525006), the Science and Technology Commission of Shanghai Municipality (07XD14022, 06PJ14068), ATIP program and BNP PARIBAS.
文摘The Small ubiquitin-related modifier (SUMO) conjugation to a variety of proteins regulates diverse cellular processes, including transcription, cell cycle regulation and maintenance of genome integrity. To investigate in vivo biological function of SUMO paralogs, we inactivated them in the early development of zebrafish. While zebrafish embryos deficient for all three SUMO paralogs, as Ubc9-deficient ones, displayed severe defects, loss of individual SUMO paralog was compatible with a normal development. SUMO-deficient embryos can be rescued by a single human or zebrafish SUMO. While key structural basic lysine residues and N-terminal unstructured stretch of SUMO are critical for in vivo rescue, the consensus Kll sumoylation site of SUMO2 is dispensable, implying that chain formation on this potential site is unessential for normal development. Inactivation of all three SUMOs triggered p53- dependent apoptosis and further inactivation of p53 restored normal zebrafish development. Interestingly, we also demonstrate that the dominant negative truncated form of p53, Δ113p53, significantly blunts SUMO depletion-induced p53 activity in vivo. Taken together, our results suggest that SUMO paralogs are indispensable, but redundant, in the early development of zebrafish.
基金the funding from the Ministry of Environment and Forests (India)
文摘This study investigated the embryonic development of Maldives clownfish Amphiprion nigripes under natural conditions (28 - 30℃) at a lagoon of Agatti Island, Lakshadweep, India. The newly deposited fish egg was capsule-shaped and orange-red, with a (0.73 ± 0.04) mm3 yolk containing 5 - 10 fat globules. The embryonic development of fertilized eggs was divided into 26 stages and the time elapsing for each stage was recorded. Results showed that the cleavage was rapid, with the first division observed 1 h 20 min after fertilization. Blastulation occurred 4 h later, followed by gastrulation 12 h after fertilization, with a yolk volume of (0.61 ± 0.06) mm3. The organogenesis process started 22 h after fertilization when the blastopores closed and notochord formation began. The embryonic stage was recorded 24 h later, with the appearance of forebrain, midbrain, hindbrain, melanophores on yolk-sac and 22 somites, and a decreased yolk volume of (0.54 ± 0.08) mm3. Other organs developed well 31 h after fertilization, whereas the heart started beating and blood circulation began 78 h later. Red pigmentation (erytrophores) appeared 96 h after fertilization, with a small yolk volume of (0.22 ± 0.02) mm3. Mouth developed well and eyes were noticeable 120 h later, with head, pectoral fin and tail frequently moving 144 h after fertilization. The embryo reached the pre-hatching stage 168 h later and started to hatch after 170 - 180 h incubation. This study first detailed the embryonic development and yolk absorption of A. nigripes under natural conditions.
基金Supported by Program for Potato Innovative Research Team in Sichuan ProvinceScience and Technology Support Program of Nanchong City~~
文摘[Objective] This study was to investigate the effects of fulvic acid on agronomic traits and yield of autumn potato. [Method] The effects of fulvic acid on growth period, plant morphology, tuber, net photosynthetic rate, chlorophyll content, leaf area index (LAI) and yield of autumn potato were investigated. [Result] The application of fulvic acid prolonged the growth period and increased the plant height, stem diameter, leaf chlorophyll content, LAI, net photosynthetic rate, tuber number per plant, tuber weight per plant and marketable tuber rate of autumn potato, thus increasing the yield. Applying fulvic acid prolonged the growth period of autumn potato by 1-3 d and significantly increased the plant height, net photosynthetic rate, tuber weight per plant and yield of autumn potato (P〈0.05, P〈0.01). When the application amount of compound fertilizer was reduced by half, applying fulvic acid almost showed the same seedling emergence stage, mature stage, growth period and net photosynthetic rate with applying full amount of compound fertilizer. In particular, no significant difference was found in the yield of autumn potato between fullamount and half-amount compound fertilizer treatment groups in the premise of solid- liquid fulvic acid combined application. [Conclusion] In the premise of unchanged yield, applying fulvic acid can reduce the application of chemical fertilizers and reduce the non-point source pollution of chemical fertilizers.
文摘Cyclin-dependent kinase 7 (Cdk7) is the catalytic subunit of the metazoan Cdk-activating kinase (CAK). Activation of Cdk7 requires its association with a regulatory subunit, Cyclin H. Although the Cdk7/Cyclin H complex has been implicated in the regulation ofRNA polymerase in several species, the precise function of their orthologs in zebrafish has not been fully elucidated. In this study, we isolated from zebrafish blastula embryos two cDNAs encoding the orthologs of human Cyclin H and Cdk7, and examined the role of Cdk7/Cyclin H in zebrafish embryogenesis. Sequence analysis showed that the zebrafish Cyclin H and Cdk7 cDNAs encode proteins with 65% and 86% identity to the respective hu- man orthologs. RT-PCR and whole-mount in situ hybridization analyses of their expression in unfertilized eggs, embryos and organs of adult fish suggested that Cyclin H and Cdk7 messages are maternally loaded. Our data also showed that their transcripts were detected throughout development. Distribution of Cyclin H transcripts was found to be ubiquitous during early stages of development and become restricted to the anterior neural tube, brain, eyes, procreate tissues, liver and heart by 5 days post-fertilization. Expression of a dominant-negative form of Cyclin H delayed the onset of zygotic transcription in the early embryo, resulting in apoptosis at 5 hours post-fertilization and leading to sever defects in tis- sues normally exhibiting high levels of Cyclin H expression. These results implicate Cyclin H in the regulation of the transcriptional machinery during midblastula transition and suggest that it is an essential gene in early zebrafish larval development.
文摘In its effort towards becoming a developed nation in the next decade, Malaysia has incorporated its education system with National Higher Education Action Plan initiatives to produce a next generation of populace who are no more dependent on the government for various aids. For the past decade, creation of entrepreneurs through a formal education has become the mainstay of the local Higher Education Institutions (HEI). Pursuing of an entrepreneurship education by means of a degree programme has emerged as one of the popular choice among undergraduates as an option for rewarding and full time career. Because of this. there has been an increased demand for entrepreneurship education and training in Malaysia to nurture entrepreneurial characteristics. The national agenda is to encourage the younger generations to be nurtured into becoming job-creators rather than job-seekers once they leave the educational system. Given this trend, HEIs need to undertake some policy changes, including faculty or school overall legislation and the development of alternative specialized programs, to be more accommodating to future entrepreneurs. This paper provides an insight into entrepreneurship education, as one of the programs that can generate interest among undergraduates to enhance entrepreneurial characteristics in order to create good entrepreneurs.
文摘The objectives of this study arc: first, to obtain a description that a political party website is an effective means of communication to carry out socialization programs to members ofthc party or the party's virtual public; second, to obtain a description that websitc is a useful means to give political education about people's political rights; third, to explore the implication of web's content for strengthening the political party's identity. The research approach used was interpretive approach to qualitative-descriptive methods. Interpretive method emphasizes culture from the perspective of social construction theory (Papa, Danicls, & Spiker, 2008). Data collection (Mann & Stewart, 2000) conducted through site analysis of the People Justice's Party (Parti Kcadilan Rakyat) Malaysia website from January 16 to March 31 2010. The findings of this study arc the website is an effective means to disseminate political party's program. Although, the virtual public or party members were preferring a direct face-to-face interpersonal communication. The People Justice's Party, Malaysia has 14 MPN (Majclis Pimpinan Negeri/Rcgional Board) in the state-level. Regarding the political atmosphere in Malaysia, the wcbsite was an efficient tool. The costs required were very cheap. Second, the presence of party website opened a space for providing political education to more people. Third, the public responses stimulated improvement and strengthening the political party's identity.
文摘Teachers of CLD (culturally and linguistically diverse) public school students New York and Rome, Italy were interviewed about their work with this unique, growing subset of learners. The 12 teachers' stories highlight myriad issues facing educators across the globe. Each teacher had recently participated in a research-based and government-sponsored professional development program focused on second language acquisition and culturally responsive pedagogy. Themes emerged through the analysis of individual interviews and classroom observations. These findings highlight teachers' perceptions of effective professional development, along with identification of institutional barriers impeding the delivery of best practices to CLD students. Cross-cultural comparisons are made which suggest an inconsistency in educational services for CLD students, along with an urgent need for professional development courses for teachers and administrators. The educators' voices shed light on both the challenges and rewards associated with this important work.