目的分析苦参素对胸腔镜术中单肺通气相关肺损伤的保护作用,探讨并完善单肺通气相关肺损伤的防治措施。方法将97例胸腔镜下肺癌根治术非小细胞肺癌患者按照随机数字表法分为观察组50例、对照组47例,2两组患者采取相同的麻醉方式,观察组...目的分析苦参素对胸腔镜术中单肺通气相关肺损伤的保护作用,探讨并完善单肺通气相关肺损伤的防治措施。方法将97例胸腔镜下肺癌根治术非小细胞肺癌患者按照随机数字表法分为观察组50例、对照组47例,2两组患者采取相同的麻醉方式,观察组在麻醉前30 min给予苦参素注射液2 m L溶于100 m L生理盐水中静脉注射,对照组患者于手术麻醉前30 min给予100 m L生理盐水静脉注射,比较2组患者麻醉诱导前（T0）、OLV即刻（T1）、OLV 60 min后（T2）、OLV 120 min后（T3）、膨肺后（T4）以及术后24 h（T5）,各时间肺内分流率（Qs/Qt）、黄嘌呤氧化物（XOD）、髓过氧化物酶（MPO）、超氧化物歧化酶（SOD）及一氧化氮（NO）水平。结果 2组患者T1~T4各时间点肺内分流率均较T0显著升高,差异具有统计学意义（P〈0.05）,不同时间2组肺内分流率比较,差异无统计学意义（P〉0.05）;2组患者T2~T5血PMN计数均较T0显著升高,差异具有统计学意义（P〈0.05）,且对照组T2~T5血PMN计数显著高于观察组,差异具有统计学意义（P〈0.05）;2组患者T2~T4血清XOD、MPO、SOD水平均较T0显著升高,差异具有统计学意义（P〈0.05）,且对照组T2~T4血清XOD、MPO、SOD水平均显著高于观察组,差异具有统计学意义（P〈0.05）;T2~T4时间2组血清NO水平较T0显著升高,差异具有统计学意义（P〈0.05）,且对照组T2~T4血清NO水平均显著低于观察组,差异具有统计学意义（P〈0.05）。结论苦参素预处理对胸腔镜术中单肺通气肺损伤具有一定的保护作用,其通过降低PMN、XOD以及MPO水平减少单肺通气患者的肺损伤,同时缓解机体单肺通气时的氧化应激水平,促进NO释放从而对肺组织起到保护作用。展开更多
AIM： To investigate whether therapeutic treatment with melatonin could protect rats against acute pan- creatitis and its associated lung injury. METHODS： Seventy-two male Sprague-Dawley rats were randomly divided in...AIM： To investigate whether therapeutic treatment with melatonin could protect rats against acute pan- creatitis and its associated lung injury. METHODS： Seventy-two male Sprague-Dawley rats were randomly divided into three groups： the sham op- eration （SO）, severe acute pancreatitis （SAP）, and mel- atonin treatment （MT） groups. Acute pancreatitis was induced by infusion of 1 mL/kg of sodium taurocholate （4% solution） into the biliopancreatic duct. Melatonin （50 mg/kg） was administered 30 min before pancre- atitis was induced, and the severity of pancreatic and pulmonary injuries was evaluated 1, 4 and 8 h after induction. Serum samples were collected to measure amylase activities, and lung tissues were removed to measure levels of mRNAs encoding interleukin 22 （IL-22） and T helper cell 22 （Th22）, as well as levels of IL-22.ing IL-22 and Th22 were significantly higher （P 〈 0.001） in the MT group than in the SAP group （0.526 ± 0.143 vs 0.156 ± 0.027, respectively, here and throughout, after 1 h; 0.489 ± 0.150 vs 0.113 ± 0.014 after 4 h; 0.524 ± 0.168 vs 0.069 ± 0.013 after 8 h, 0.378 ± 0.134 vs 0.122 ± 0.015 after 1 h; 0.205 ± 0.041 vs 0.076 ± 0.019 after 4 h; 0.302 ± 0.108 vs 0.045 ± 0.013 after 8 h, respectively） and significantly lower （P 〈 0.001） in the SAP group than in the SO group （0.156 ± 0.027 vs 1.000 ± 0.010 after 1 h; 0.113 ± 0.014 vs 1.041 ± 0.235 after 4 h; 0.069 ± 0.013 vs 1.110 ± 0.213 after 8 h, 0.122 ± 0.015 vs 1.000 ± 0.188 after 1 h; 0.076 ± 0.019 vs 0.899 ± 0.125 after 4 h; 0.045 ± 0.013 vs 0.991 ± 0.222 after 8 h, respectively）. The mean pathologi- cal scores for pancreatic tissues in the MT group were significantly higher （P 〈 0.01） than those for samples in the SO group （1.088 ± 0.187 vs 0.488 ± 0.183 after 1 h, 2.450 ± 0.212 vs 0.469 ± 0.242 after 4 h; 4.994 ± 0.184 vs 0.513 ± 0.210 after 8 h）, but were significantly lower （P 〈 0.01） than those for samples in the SAP group at each time point （1.088 ± 0.187 vs 1.969 ± 0.290 after 1 h; 2.450 ± 0.212 vs 3.344 ± 0.386 after 4 h; 4.994 ± 0.184 vs 6.981 ± 0.301 after 8 h）. The severity of SAP increased significantly （P 〈 0.01） over time in the SAP group （1.088 ± 0.187 vs 2.450 ± 0.212 between 1 h and 4 h after inducing pancreatitis; and 2.450 ± 0.212 vs 4.994 ± 0.184 between 4 and 8 h after inducing pan- creatitis）. CONCLUSION： Melatonin protects rats against acute pancreatitis-associated lung injury, probably through the upregulation of IL-22 and Th22, which increases the innate immunity of tissue cells and enhances their regeneration.展开更多
Objective. To determine the effect of albumin administration on lung injury in traumatic/hemorrhagic shock （T/HS） rats. Methods： Forty-eight adult Sprague-Dawley rats were divided into three groups randomly （ n =...Objective. To determine the effect of albumin administration on lung injury in traumatic/hemorrhagic shock （T/HS） rats. Methods： Forty-eight adult Sprague-Dawley rats were divided into three groups randomly （ n = 16 in each group） ： Group A, Group B, Group C. In Group A, rats underwent laparotomy without shock. In Group B, rats undergoing T/HS were resuscitated with their blood plus lactated Ringer＇s （twice the volume of shed blood ）. In Group C, rats undergoing T/HS were resuscitated with their shed blood plus additional 3 ml of 5% human albumin. The expression of polymorphonuclear neutrophils CD18/CD11b in jugular vein blood was evaluated. The main lung injury indexes （the activity of myeloperoxidase and lung injury score） were measured. Results： Significant differences of the expression of CD18/11b and the severity degree of lung injury were found between the three groups. （P〈0.05）. The expression of CD18/CD11b and the main lung injury indexes in Group B and Goup C incresed significantly compared with those in Group A （P 〈0.05）. At the same time, the expression of CD18/CD11b and the main lung injury indexes in Group C decreased dramatically, compared with those in Group B （ P 〈0.05 ）. Conclusions ： The infusion of albumin during resuscitation period can protect lungs from injury and decrease the expression of CD18/CD11b in T/HS rats.展开更多
文摘目的分析苦参素对胸腔镜术中单肺通气相关肺损伤的保护作用,探讨并完善单肺通气相关肺损伤的防治措施。方法将97例胸腔镜下肺癌根治术非小细胞肺癌患者按照随机数字表法分为观察组50例、对照组47例,2两组患者采取相同的麻醉方式,观察组在麻醉前30 min给予苦参素注射液2 m L溶于100 m L生理盐水中静脉注射,对照组患者于手术麻醉前30 min给予100 m L生理盐水静脉注射,比较2组患者麻醉诱导前（T0）、OLV即刻（T1）、OLV 60 min后（T2）、OLV 120 min后（T3）、膨肺后（T4）以及术后24 h（T5）,各时间肺内分流率（Qs/Qt）、黄嘌呤氧化物（XOD）、髓过氧化物酶（MPO）、超氧化物歧化酶（SOD）及一氧化氮（NO）水平。结果 2组患者T1~T4各时间点肺内分流率均较T0显著升高,差异具有统计学意义（P〈0.05）,不同时间2组肺内分流率比较,差异无统计学意义（P〉0.05）;2组患者T2~T5血PMN计数均较T0显著升高,差异具有统计学意义（P〈0.05）,且对照组T2~T5血PMN计数显著高于观察组,差异具有统计学意义（P〈0.05）;2组患者T2~T4血清XOD、MPO、SOD水平均较T0显著升高,差异具有统计学意义（P〈0.05）,且对照组T2~T4血清XOD、MPO、SOD水平均显著高于观察组,差异具有统计学意义（P〈0.05）;T2~T4时间2组血清NO水平较T0显著升高,差异具有统计学意义（P〈0.05）,且对照组T2~T4血清NO水平均显著低于观察组,差异具有统计学意义（P〈0.05）。结论苦参素预处理对胸腔镜术中单肺通气肺损伤具有一定的保护作用,其通过降低PMN、XOD以及MPO水平减少单肺通气患者的肺损伤,同时缓解机体单肺通气时的氧化应激水平,促进NO释放从而对肺组织起到保护作用。
文摘AIM： To investigate whether therapeutic treatment with melatonin could protect rats against acute pan- creatitis and its associated lung injury. METHODS： Seventy-two male Sprague-Dawley rats were randomly divided into three groups： the sham op- eration （SO）, severe acute pancreatitis （SAP）, and mel- atonin treatment （MT） groups. Acute pancreatitis was induced by infusion of 1 mL/kg of sodium taurocholate （4% solution） into the biliopancreatic duct. Melatonin （50 mg/kg） was administered 30 min before pancre- atitis was induced, and the severity of pancreatic and pulmonary injuries was evaluated 1, 4 and 8 h after induction. Serum samples were collected to measure amylase activities, and lung tissues were removed to measure levels of mRNAs encoding interleukin 22 （IL-22） and T helper cell 22 （Th22）, as well as levels of IL-22.ing IL-22 and Th22 were significantly higher （P 〈 0.001） in the MT group than in the SAP group （0.526 ± 0.143 vs 0.156 ± 0.027, respectively, here and throughout, after 1 h; 0.489 ± 0.150 vs 0.113 ± 0.014 after 4 h; 0.524 ± 0.168 vs 0.069 ± 0.013 after 8 h, 0.378 ± 0.134 vs 0.122 ± 0.015 after 1 h; 0.205 ± 0.041 vs 0.076 ± 0.019 after 4 h; 0.302 ± 0.108 vs 0.045 ± 0.013 after 8 h, respectively） and significantly lower （P 〈 0.001） in the SAP group than in the SO group （0.156 ± 0.027 vs 1.000 ± 0.010 after 1 h; 0.113 ± 0.014 vs 1.041 ± 0.235 after 4 h; 0.069 ± 0.013 vs 1.110 ± 0.213 after 8 h, 0.122 ± 0.015 vs 1.000 ± 0.188 after 1 h; 0.076 ± 0.019 vs 0.899 ± 0.125 after 4 h; 0.045 ± 0.013 vs 0.991 ± 0.222 after 8 h, respectively）. The mean pathologi- cal scores for pancreatic tissues in the MT group were significantly higher （P 〈 0.01） than those for samples in the SO group （1.088 ± 0.187 vs 0.488 ± 0.183 after 1 h, 2.450 ± 0.212 vs 0.469 ± 0.242 after 4 h; 4.994 ± 0.184 vs 0.513 ± 0.210 after 8 h）, but were significantly lower （P 〈 0.01） than those for samples in the SAP group at each time point （1.088 ± 0.187 vs 1.969 ± 0.290 after 1 h; 2.450 ± 0.212 vs 3.344 ± 0.386 after 4 h; 4.994 ± 0.184 vs 6.981 ± 0.301 after 8 h）. The severity of SAP increased significantly （P 〈 0.01） over time in the SAP group （1.088 ± 0.187 vs 2.450 ± 0.212 between 1 h and 4 h after inducing pancreatitis; and 2.450 ± 0.212 vs 4.994 ± 0.184 between 4 and 8 h after inducing pan- creatitis）. CONCLUSION： Melatonin protects rats against acute pancreatitis-associated lung injury, probably through the upregulation of IL-22 and Th22, which increases the innate immunity of tissue cells and enhances their regeneration.
文摘Objective. To determine the effect of albumin administration on lung injury in traumatic/hemorrhagic shock （T/HS） rats. Methods： Forty-eight adult Sprague-Dawley rats were divided into three groups randomly （ n = 16 in each group） ： Group A, Group B, Group C. In Group A, rats underwent laparotomy without shock. In Group B, rats undergoing T/HS were resuscitated with their blood plus lactated Ringer＇s （twice the volume of shed blood ）. In Group C, rats undergoing T/HS were resuscitated with their shed blood plus additional 3 ml of 5% human albumin. The expression of polymorphonuclear neutrophils CD18/CD11b in jugular vein blood was evaluated. The main lung injury indexes （the activity of myeloperoxidase and lung injury score） were measured. Results： Significant differences of the expression of CD18/11b and the severity degree of lung injury were found between the three groups. （P〈0.05）. The expression of CD18/CD11b and the main lung injury indexes in Group B and Goup C incresed significantly compared with those in Group A （P 〈0.05）. At the same time, the expression of CD18/CD11b and the main lung injury indexes in Group C decreased dramatically, compared with those in Group B （ P 〈0.05 ）. Conclusions ： The infusion of albumin during resuscitation period can protect lungs from injury and decrease the expression of CD18/CD11b in T/HS rats.
