AIM： To determine the effects of pentoxifylline （PTX） on clinical manifestations and evaluate arterial blood gas data in hepatopulmonary syndrome （HPS） in chil- dren. METHODS： In a pilot study of 10 children wit...AIM： To determine the effects of pentoxifylline （PTX） on clinical manifestations and evaluate arterial blood gas data in hepatopulmonary syndrome （HPS） in chil- dren. METHODS： In a pilot study of 10 children with chronic liver disease, who had HPS, 20 mg/kg/d PTX was ad- ministered for 3 mo. Clinical data and arterial blood gas parameters were evaluated at baseline, the end of the treatment period, and 3 mo after drug discontinuation. RESULTS： Six patients could tolerate PTX, while four patients experienced complications, Among patients who could tolerate PTX, there was a significant increase in arterial oxygen pressure （PaO2） （P = 0,02） and oxy- gen saturation （Sa02） （P = 0.04） and alveolar-arterial oxygen gradient （P = 0.02） after 3 mo of treatment. Significant decreases in Pa02 （P = 0.02） and alveolararterial oxygen gradient （P = 0.02） were also seen after drug discontinuation. CONCLUSION： PTX may improve PaO2, Sa02 and alve- olar-arterial oxygen gradient in the early stage of HPS.展开更多
This study aimed to establish an animal model of decompression-induced lung injury(DILI)secondary to repetitive diving in mice and explore the role of macrophages in DILI and the protective effects of high-concentrati...This study aimed to establish an animal model of decompression-induced lung injury(DILI)secondary to repetitive diving in mice and explore the role of macrophages in DILI and the protective effects of high-concentration hydrogen(HCH)on DILI.Mice were divided into three groups:control group,DILI group,and HCH group.Mice were exposed to hyperbaric air at 600 k Pa for 60 min once daily for consecutive 3 d and then experienced decompression.In HCH group,mice were administered with HCH(66.7%hydrogen and 33.3%oxygen)for 60 min after each hyperbaric exposure.Pulmonary function tests were done 6 h after decompression;the blood was harvested for cell counting;the lung tissues were harvested for the detection of inflammatory cytokines,hematoxylin and eosin(HE)staining,and immunohistochemistry;western blotting and polymerase chain reaction(PCR)were done for the detection of markers for M1 and M2 macrophages.Our results showed that bubbles formed after decompression and repeated hyperbaric exposures significantly reduced the total lung volume and functional residual volume.Moreover,repetitive diving dramatically increased proinflammatory factors and increased the markers of both M1 and M2 macrophages.HCH inhalation improved lung function to a certain extent,and significantly reduced the pro-inflammatory factors.These effects were related to the reduction of M1 macrophages as well as the increase in M2 macrophages.This study indicates that repetitive diving damages lung function and activates lung macrophages,resulting in lung inflammation.HCH inhalation after each diving may be a promising strategy for the prevention of DILI.展开更多
基金Supported by Research Council of Mashhad University of Medical Sciences
文摘AIM： To determine the effects of pentoxifylline （PTX） on clinical manifestations and evaluate arterial blood gas data in hepatopulmonary syndrome （HPS） in chil- dren. METHODS： In a pilot study of 10 children with chronic liver disease, who had HPS, 20 mg/kg/d PTX was ad- ministered for 3 mo. Clinical data and arterial blood gas parameters were evaluated at baseline, the end of the treatment period, and 3 mo after drug discontinuation. RESULTS： Six patients could tolerate PTX, while four patients experienced complications, Among patients who could tolerate PTX, there was a significant increase in arterial oxygen pressure （PaO2） （P = 0,02） and oxy- gen saturation （Sa02） （P = 0.04） and alveolar-arterial oxygen gradient （P = 0.02） after 3 mo of treatment. Significant decreases in Pa02 （P = 0.02） and alveolararterial oxygen gradient （P = 0.02） were also seen after drug discontinuation. CONCLUSION： PTX may improve PaO2, Sa02 and alve- olar-arterial oxygen gradient in the early stage of HPS.
基金Project supported by the National Natural Science Foundation of China(No.81772015)。
文摘This study aimed to establish an animal model of decompression-induced lung injury(DILI)secondary to repetitive diving in mice and explore the role of macrophages in DILI and the protective effects of high-concentration hydrogen(HCH)on DILI.Mice were divided into three groups:control group,DILI group,and HCH group.Mice were exposed to hyperbaric air at 600 k Pa for 60 min once daily for consecutive 3 d and then experienced decompression.In HCH group,mice were administered with HCH(66.7%hydrogen and 33.3%oxygen)for 60 min after each hyperbaric exposure.Pulmonary function tests were done 6 h after decompression;the blood was harvested for cell counting;the lung tissues were harvested for the detection of inflammatory cytokines,hematoxylin and eosin(HE)staining,and immunohistochemistry;western blotting and polymerase chain reaction(PCR)were done for the detection of markers for M1 and M2 macrophages.Our results showed that bubbles formed after decompression and repeated hyperbaric exposures significantly reduced the total lung volume and functional residual volume.Moreover,repetitive diving dramatically increased proinflammatory factors and increased the markers of both M1 and M2 macrophages.HCH inhalation improved lung function to a certain extent,and significantly reduced the pro-inflammatory factors.These effects were related to the reduction of M1 macrophages as well as the increase in M2 macrophages.This study indicates that repetitive diving damages lung function and activates lung macrophages,resulting in lung inflammation.HCH inhalation after each diving may be a promising strategy for the prevention of DILI.
