Objective:To elucidate the expression differences of vascular growth factors in human lung adenocarcinoma cell line A549 and cisplatin-resistant human lung adenocarcinoma cell line A549^DDP.Methods:RT-PCR and immunohi...Objective:To elucidate the expression differences of vascular growth factors in human lung adenocarcinoma cell line A549 and cisplatin-resistant human lung adenocarcinoma cell line A549^DDP.Methods:RT-PCR and immunohistochemistry was used to detect the mRNA and protein expressions of vascular endothelial growth factor(VEGF)and basic fibroblast growth factor (bFGF) in A549 and A549^DDP.Results:VEGF and bFGF mRNA were expressed in A549 and in A549^DDP.VEGF and bFGF mRNA expression levels in A549^DDP were signifi-cant higher than those in A549(P<0.025).VEGF and bFGF protein expressions were all strong positive in A549 and A549^DDP.Conclusion:There are certain differences between VEGF and bFGF expressions in A549 and A549^DDP.Drug-resistance of lung cancer is associated with those above genes over-expressions.Over-expression of vascular growth factors are related to drug resistance of lung cancer.展开更多
Human antisense GST-πRNA was transferred into adriamycine-treatedadenocarcinoma cell of lung. The over-expression of GST-πgene wasinhibited and the total GST activity towards CDNB, the test substrate, wasdecreased. ...Human antisense GST-πRNA was transferred into adriamycine-treatedadenocarcinoma cell of lung. The over-expression of GST-πgene wasinhibited and the total GST activity towards CDNB, the test substrate, wasdecreased. Transfected cells showed elevated sensitivity to adriamycine ascompared with un-transfected cells. The results suggest that GST-π plays arole in drug resistance of human adenocarcinoma of lung, and inhibition ofGST eazymes might partially restore the sensitivity of cancer tochemotherapy.展开更多
基金Supported by the grant from Beijing Natural Science Foundation(No.7992005)Science,Technology New Star Plan of Beijing(No.148)and China‘9.5’research item(96-906-01-23)
文摘Objective:To elucidate the expression differences of vascular growth factors in human lung adenocarcinoma cell line A549 and cisplatin-resistant human lung adenocarcinoma cell line A549^DDP.Methods:RT-PCR and immunohistochemistry was used to detect the mRNA and protein expressions of vascular endothelial growth factor(VEGF)and basic fibroblast growth factor (bFGF) in A549 and A549^DDP.Results:VEGF and bFGF mRNA were expressed in A549 and in A549^DDP.VEGF and bFGF mRNA expression levels in A549^DDP were signifi-cant higher than those in A549(P<0.025).VEGF and bFGF protein expressions were all strong positive in A549 and A549^DDP.Conclusion:There are certain differences between VEGF and bFGF expressions in A549 and A549^DDP.Drug-resistance of lung cancer is associated with those above genes over-expressions.Over-expression of vascular growth factors are related to drug resistance of lung cancer.
文摘Human antisense GST-πRNA was transferred into adriamycine-treatedadenocarcinoma cell of lung. The over-expression of GST-πgene wasinhibited and the total GST activity towards CDNB, the test substrate, wasdecreased. Transfected cells showed elevated sensitivity to adriamycine ascompared with un-transfected cells. The results suggest that GST-π plays arole in drug resistance of human adenocarcinoma of lung, and inhibition ofGST eazymes might partially restore the sensitivity of cancer tochemotherapy.
