Objective: The aim of this study was to use lung cancer targeting binding polypeptide ZS-9 to screen cDNA library of human lung cancer and obtain ZS-9 specific ligand to confirm tumor marker of non small-cell lung can...Objective: The aim of this study was to use lung cancer targeting binding polypeptide ZS-9 to screen cDNA library of human lung cancer and obtain ZS-9 specific ligand to confirm tumor marker of non small-cell lung cancer. Methods: Artificially synthesize biotin labeled peptide ZS-9, anchored ZS-9 in the enzyme label plate coupled by avidin, used ZS-9 as probe to screen cDNA library of human lung cancer, after screening, obtained bacteriophage clone specifically binding with anchored polypeptide ZS-9. Extracted plasmid of bacteriophage and performed sequencing after amplified by PCR. Results: It was demonstrated by bioinformatic analysis on the sequence of ligand binded by lung cancer specific peptide ZS-9 that the ligand was the cytoskeletal protein periplakin on the surface of lung cancer cells, suggesting that periplakin might be a new marker for non-small-cell lung cancer in lung cancer. Conclusion: Use specific lung cancer binding peptide to screen new tumor marker periplakin in lung cancer and further studies on its biologic functions in genesis and development of lung cancer are still needed.展开更多
肺癌干细胞(lung cancer stem cell,LCSC)是指肺癌组织中一部分具备自我更新和增殖分化能力、致瘤性较强、并具有特异性标记物的细胞群。它们在肺癌的发生、发展、侵袭、转移、复发以及抗辐射和耐药性获得起至关重要的作用。近年来多种...肺癌干细胞(lung cancer stem cell,LCSC)是指肺癌组织中一部分具备自我更新和增殖分化能力、致瘤性较强、并具有特异性标记物的细胞群。它们在肺癌的发生、发展、侵袭、转移、复发以及抗辐射和耐药性获得起至关重要的作用。近年来多种肺癌干细胞标记物相继被发现和报道,人们对肺癌干细胞的生物学功能有了更深入的认识。本文对肺癌干细胞的起源和发现、肺癌干细胞的生物学功能、肺癌干细胞的分选和鉴定、肺癌干细胞标记物等方面进行综述。展开更多
基金Supported by grants from the Science and Technology Planning Project of Guangdong Province (No. 2010B031600066 No. 2010B031500034+2 种基金 No. 2008B030303008)the Key Scientific Subject Foundation of Ministry of Education of China (No. 208105)the National Science and Technology Major Projects for New Drugs (No. 2011zx09102-001-31)
文摘Objective: The aim of this study was to use lung cancer targeting binding polypeptide ZS-9 to screen cDNA library of human lung cancer and obtain ZS-9 specific ligand to confirm tumor marker of non small-cell lung cancer. Methods: Artificially synthesize biotin labeled peptide ZS-9, anchored ZS-9 in the enzyme label plate coupled by avidin, used ZS-9 as probe to screen cDNA library of human lung cancer, after screening, obtained bacteriophage clone specifically binding with anchored polypeptide ZS-9. Extracted plasmid of bacteriophage and performed sequencing after amplified by PCR. Results: It was demonstrated by bioinformatic analysis on the sequence of ligand binded by lung cancer specific peptide ZS-9 that the ligand was the cytoskeletal protein periplakin on the surface of lung cancer cells, suggesting that periplakin might be a new marker for non-small-cell lung cancer in lung cancer. Conclusion: Use specific lung cancer binding peptide to screen new tumor marker periplakin in lung cancer and further studies on its biologic functions in genesis and development of lung cancer are still needed.
文摘肺癌干细胞(lung cancer stem cell,LCSC)是指肺癌组织中一部分具备自我更新和增殖分化能力、致瘤性较强、并具有特异性标记物的细胞群。它们在肺癌的发生、发展、侵袭、转移、复发以及抗辐射和耐药性获得起至关重要的作用。近年来多种肺癌干细胞标记物相继被发现和报道,人们对肺癌干细胞的生物学功能有了更深入的认识。本文对肺癌干细胞的起源和发现、肺癌干细胞的生物学功能、肺癌干细胞的分选和鉴定、肺癌干细胞标记物等方面进行综述。
