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IL-18通过下调CXCL9/CXCL10募集肿瘤相关巨噬细胞促进口腔鳞癌病灶转移 被引量:4
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作者 周卫 曾华兴 肖才文 《中国免疫学杂志》 CAS CSCD 北大核心 2021年第10期1221-1225,共5页
目的:探讨IL-18在口腔鳞癌(OSCC)患者肿瘤组织中的表达水平及肿瘤相关巨噬细胞(TAMs)对OSCC肺转移病灶形成的影响。方法:本研究共纳入2015年1月至2017年12月我院收治的38例OSCC患者,另选取47例同期在我院体检中心进行体检的健康志愿者... 目的:探讨IL-18在口腔鳞癌(OSCC)患者肿瘤组织中的表达水平及肿瘤相关巨噬细胞(TAMs)对OSCC肺转移病灶形成的影响。方法:本研究共纳入2015年1月至2017年12月我院收治的38例OSCC患者,另选取47例同期在我院体检中心进行体检的健康志愿者作为对照。ELISA法检测各组研究对象血清IL-18水平;免疫组化和Western blot分析OSCC患者肿瘤组织和癌旁组织中IL-18表达。建立小鼠肺转移模型,采用慢病毒转染法构建OSCC Tca8113 IL-18高表达细胞系(Tca8113-IL-18),Western blot检测IL-18、AKT、P-AKT、mTOR和P-mTOR表达。裸鼠尾静脉注射Tca8113与Tca8113-IL-18细胞,45 d后统计小鼠肺组织转移病灶数,CD11b和F4/80标记后流式细胞术分选巨噬细胞;ELISA检测小鼠血清中CXCL9、CXCL10、CSF-1和CCL-2表达;qRT-PCR检测小鼠肿瘤组织CXCL9、CXCL10、CSF-1和CCL-2表达;Western blot检测CXCL9、CXCL10、AKT、P-AKT、mTOR和P-mTOR表达。结果:OSCC患者血清中IL-18表达显著高于健康志愿者,肿瘤组织中IL-18表达显著高于癌旁组织(P<0.05)。IL-18在Tca8113-IL-18高表达,成功构建高表达细胞系,P-AKT和P-mTOR在Tca8113-IL-18细胞系中表达高于Tca8113细胞。IL-18可显著增加Tca8113在裸鼠肺部形成转移病灶数(P<0.05),Tca8113-IL-18组裸鼠肺部转移瘤数明显高于Tca8113组。流式细胞术分选结果显示,Tca8113-IL-18组裸鼠肿瘤组织中TAMs数明显增多。ELISA结果显示Tca8113-IL-18裸鼠血清中CXCL9和CXCL10表达明显低于Tca8113组,而CSF-1和CCL-2表达明显升高。qRT-PCR和Western blot显示Tca8113-IL-18肿瘤组织中CXCL9和CXCL10表达明显低于Tca8113组,而CSF-1和CCL-2表达明显增加,Tca8113-IL-18组P-AKT和P-mTOR表达高于Tca8113组。结论:OSCC患者血清和肿瘤组织中IL-18表达显著升高,IL-18可能通过激活PI3K/AKT/mTOR信号通路下调CXCL9、CXCL10表达,从而募集TAMs促进OSCC肺部转移病灶形成。 展开更多
关键词 IL-18 CXCL9/CXCL10 口腔鳞癌 肺转移病灶
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Cavitary Pulmonary Metastases: CT Features and Their Correlation with the Pathology of the Primary Malignancy 被引量:1
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作者 于小平 王平 梁赵玉 《The Chinese-German Journal of Clinical Oncology》 CAS 2004年第1期29-33,66,共6页
Objective: To study CT features of cavitary pulmonary metastases and to investigate the pos- sible relationship between CT features and the pathology of the primary lesions. Methods: CT ?ndings o... Objective: To study CT features of cavitary pulmonary metastases and to investigate the pos- sible relationship between CT features and the pathology of the primary lesions. Methods: CT ?ndings of 131 cavitary metastatic nodules in 40 patients with pathologically-proved pulmonary metastases were retrospectively analyzed. A comparison between CT signs and the pathologic types of the primary tumors was made. Results: Cavitary metastases and multiple solid nodules coexisted in all patients. Cavitary metastases presented as bubble (n=41), irregular (n=33), cystic (n=26) or small circular (n=31) cavities, with even (n=61) or uneven (n=70) thickness of the cavity wall. Of 131 cavitary nodules, diameter less than 15 mm was seen in 44, between 15–25 mm in 66, 25–40 mm in 17 and larger than 40 mm in 4 respectively. And the wall thickness of the cavity below 4 mm, between 4–15 mm and over 15 mm was respectively seen in 69, 44 and 18 metastatic nodules. Cavitary pulmonary metastases mainly occurred in patients whose primary malignancy was squamous cell carcinoma (n=13) or adenocarcinoma (n=22). Both squamous cell carcinoma and adenocarcinoma had its own CT characteristics. The occurrence of cavity bore no relationship to its site in the lung. Conclusion: Cavitary pulmonary metastases carries certain CT features and its occurrence is related to the pathologic type of the primary malignancy. 展开更多
关键词 lung neoplasm metastatic tomography X-ray computed PATHOLOGY
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