Objective. To approach the relation and the possible mechanism between the expression of intercellular adhesion molecule (ICAM1) mRNA and acute lung injury (ALI) and the mechanisms of rhubarb in the prevention and tre...Objective. To approach the relation and the possible mechanism between the expression of intercellular adhesion molecule (ICAM1) mRNA and acute lung injury (ALI) and the mechanisms of rhubarb in the prevention and treatment of the lung injury. Methods. Lipopolysaccharide (LPS) was injected into the sublingual vein of male Wistar rats to perform ALI animal model. The rats were divided into 4 groups: LPS group, control group, rhubarb group and dexamethasone group. Macroscopic and histopathological examinations were performed and biological markers were measured for the lung specimens. The markers included lung wet/dry weight, the rate of neutrophils and protein content in the pulmonary alveolar lavage fluid, pulmonary vascular permeability and pulmonary alveolar permeability index. Molecular hybridization method was used to determine the expression of ICAM1 mRNA. Results. In the lung tissues, the ICAM1 mRNA expression was increased in the endothelial cells of pulmonary veins and capillaries, rhubarb and dexamethasone had the action of decreasing the expression. The light reflex value in the gray scale scanning showed that in the comparison between the LPS and the control group, the gray scale value of the lung tissues in ALI was significantly increased, thus the light reflex value was markedly decreased (P<001), demonstrating the expression of ICAM1 mRNA was increased. In comparison with the LPS group, dexamethasone and rhubarb could decrease the gray scale value of the lung tissue significantly, thus the light reflex value was elevated (P<001, P<005); the corresponding pathologic changes of lung tissues and the biological markers of the lung injury were significantly decreased or ameliorated. Conclusions. The increase of the expression of ICAM1 mRNA in the lung tissues of ALI plays the roles in ALI. The application of rhubarb and dexamethasone can decrease the expression and ameliorate the lung damage; its mechanism is possibly via the inhibition of ICAM1 mRNA expression.展开更多
Purpose The warming-Yang or cooling-heat is the principle rules of traditional Chinese medicine theory. The purpose of this study is to identify the effect of warming-Yang or cooling-heat Chinese medicine prescription...Purpose The warming-Yang or cooling-heat is the principle rules of traditional Chinese medicine theory. The purpose of this study is to identify the effect of warming-Yang or cooling-heat Chinese medicine prescriptions combining with Gefitinib on lung cancer H1650 cell line. Materials and Methods： The typical warming-Yang prescription, Shen-fu （SF） Injection, and cooling-heat prescription, Qing-kai-ling （QKL） Injection was combined with Gefitinib respectively to treat H1650 cells. Cell viability was measured by MTT assay and Annexin V/PI method. Epidermal growth factor receptor （EGFR） pathway proteins were detected by Western blot. Results： QKL combined with Gefitinib significantly suppressed cell proliferation and induced apoptosis in H1650 cell line compared with single drug （P 〈 0.05）. On the contrary, SF and Gefitinib had significant antagonism effects on both cell viability inhibition and apoptosis （48h： 0.85 〈 Q 〈 1.15, 72h： Q 〈 0.85）. Although higher EGFR protein level was observed in Gefitinib group, which was significantly reduced in QKL ＋ Gefitinib group, no significant differences were observed in the levels of p-EGFR （P 〉 0.05）, downstream AKT/p-AKT （P 〉 0.05）, or ERK/p-ERK （P 〉 0.05） among Gefitinib, Gefitinib ＋ SF, and Gefitinib ＋ QKL groups. Conclusion： QKL Injection may reduce the level of EGFR and then improve the resistance of H1650 cells to EGFR-TKIs, which may be the mechanism of synergism effect of QKL Injection and Gefitinib. The principle rules of warming-Yang or cooling-heat should be further elucidated, and more research are required to explore the combined effect of Chinese medicine with opposite principle and Gefitinib.展开更多
Objective Radiation-induced lung injury （RILl） is the most common, dose-limiting complication in thoracic malignancy radiotherapy. Considering its negative impact on patients and restrictions to efficacy, the mechan...Objective Radiation-induced lung injury （RILl） is the most common, dose-limiting complication in thoracic malignancy radiotherapy. Considering its negative impact on patients and restrictions to efficacy, the mechanism of RILl was studied. Methods Wistar rats were locally irradiated with a single dose of 0, 16, and 20 Gy to the right half of the lung to establish a lung injury model. Two and six months after irradiation, the right half of the rat lung tissue was removed, and the concentrations of TGF-[31, angiotensin II, and aldosterone were determined via enzyme-linked immunosorbent assay. Results Statistical differences were observed in the expression levels of angiotensin II and aldosterone between the non-irradiation and irradiation groups. Moreover, the expression level of the angiotensin II-aldosterone system increased with increasing doses, and the difference was still observed as time progressed. Conclusions Angiotensin II-aldosterone system has an important pathophysiological function in the progression of RILI.展开更多
Objective： The aim of the study was to investigate whether enhanced anti-tumor effects of endostar （ES） on radiation involved hypoxia inducible factor-la （HIF-la） and basic ~broblast growth factor （bFGF）. Meth...Objective： The aim of the study was to investigate whether enhanced anti-tumor effects of endostar （ES） on radiation involved hypoxia inducible factor-la （HIF-la） and basic ~broblast growth factor （bFGF）. Methods： A549 cells were divided into four groups： control group; endostar （ES） group; radiation （RT） group; endostar plus radiation （ES ＋ RT） group. The inhibition of proliferation rates ofA549 cells was measured by cell counting kit-8 （CCK-8）. HIF-la and bFGF expression levels were checked by enzyme linked immunosorbent assay （ELISA） and reverse transcription-polymerase chain reaction （RT-PCR）. Results： The proliferation inhibition rate in the ES ＋ RT group was higher than that in other groups. On the other hand, the expressions of HIF-1α and bFGF in the ES ＋ RT group were significantly reduced compared to other groups. HIF-1α and bFGF levels were positively correlated （r = 0.80, P 〈 0.01）. Conclusion： Our results suggest that endostar could enhance anti-tumor effect of radiation by reducing expressions of HIF-1α and bFGF.展开更多
Objective: To investigate the effect of Shen-Fu (SF) injection on gastrointestinal tract injury and its potential mechanism.Methods: Thirty-eight patients undergoing elective open heart surgery were assigned to Group ...Objective: To investigate the effect of Shen-Fu (SF) injection on gastrointestinal tract injury and its potential mechanism.Methods: Thirty-eight patients undergoing elective open heart surgery were assigned to Group C (control group, n = 18) and Group SF (n = 20) randomly. In Group SF, the patients received intravenous injection of SF (0.5 ml/kg) at the beginning of the surgery followed by a continuous infusion of 100 ml SF (1.0 ml/kg) solution diluted by saline at a rate of 0.004 ml · Kg-1 · min-1 with a Grasby pump. The control group was injected with normal saline in the same volume. Gastric intramucosal pH (pHi), activity of blood diamine oxidase ( DAO ), and concentrations of blood LPS and IL-6 were measured before CPB ( S0) and 1 h ( S1 ) and 2 h ( S2) after aortic declamping, respectively.Results: In Group C, pHi value was significantly lower at S1 and S2 than at S0 ( mean P <0.01) and blood DAO and concentrations of LPS and IL-6 were significantly higher at S1 and S2 than at S0 ( meanP < 0.01). In Group SF, pHi was obviously lower at S1 and S2 than at S0(P< 0.05) but LPS and IL-6 levels and DAO were higher at S, (mean P<0.05). Blood DAO and LPS level demonstrated significant negative correlations with pHi ( mean P < 0.01) while LPS concentration showed a positive correlation with blood DAO (P < 0.01) and IL-6 concentration (P < 0.05). At S1 and S2 after aortic declamping, the levels of pHi were higher in Group SF than in Group C (mean P <0.01 ) but DAO and LPS and IL-6 levels were significantly lower in Group SF than in Group C ( P <0.01).Conclusions: SF has a protective effect on gastrointestinal tract and can reduce inflammatory actions.展开更多
OBJECTIVE: To observe the effect of decoction for reinforcing lung Qi on T-lymphocytic function, interleukin-2 (IL-2) and tumor necrosis factor-α (TNF-α) in peripheral blood of patients with non-small cell lung canc...OBJECTIVE: To observe the effect of decoction for reinforcing lung Qi on T-lymphocytic function, interleukin-2 (IL-2) and tumor necrosis factor-α (TNF-α) in peripheral blood of patients with non-small cell lung cancer after operation with argon helium lancet in order to explore its mechanism.METHODS: A total of 76 patients suffering from non-small cell lung cancer without surgical indication were randomly divided into a treatment group treated with decoction for reinforcing lung Qi and argon helium lancet and a control group treated with argon helium lancet only to observe lymphocytic proliferation, detect the percentage of positive cells in the T-lymphocyte CD28 with flow cytometry and detect the expression of IL-2 and TNF-α in peripheral blood with enzyme linked immunosorbent assay.RESULTS: Proliferation of T-lymphocytes and expression of CD28, IL-2 and TNF-α in peripheral blood after treatment in the treatment group were more obviously strengthened than those before treatment and those in the control group (all P<0.05).CONCLUSION: The mechanism of using decoction for reinforcing lung Qi and argon helium lancet to treat lung cancer may be realized through promoting T-lymphocytic proliferation, up-regulating expression of CD28, IL-2 and TNF-α, and activating T-cells.展开更多
文摘Objective. To approach the relation and the possible mechanism between the expression of intercellular adhesion molecule (ICAM1) mRNA and acute lung injury (ALI) and the mechanisms of rhubarb in the prevention and treatment of the lung injury. Methods. Lipopolysaccharide (LPS) was injected into the sublingual vein of male Wistar rats to perform ALI animal model. The rats were divided into 4 groups: LPS group, control group, rhubarb group and dexamethasone group. Macroscopic and histopathological examinations were performed and biological markers were measured for the lung specimens. The markers included lung wet/dry weight, the rate of neutrophils and protein content in the pulmonary alveolar lavage fluid, pulmonary vascular permeability and pulmonary alveolar permeability index. Molecular hybridization method was used to determine the expression of ICAM1 mRNA. Results. In the lung tissues, the ICAM1 mRNA expression was increased in the endothelial cells of pulmonary veins and capillaries, rhubarb and dexamethasone had the action of decreasing the expression. The light reflex value in the gray scale scanning showed that in the comparison between the LPS and the control group, the gray scale value of the lung tissues in ALI was significantly increased, thus the light reflex value was markedly decreased (P<001), demonstrating the expression of ICAM1 mRNA was increased. In comparison with the LPS group, dexamethasone and rhubarb could decrease the gray scale value of the lung tissue significantly, thus the light reflex value was elevated (P<001, P<005); the corresponding pathologic changes of lung tissues and the biological markers of the lung injury were significantly decreased or ameliorated. Conclusions. The increase of the expression of ICAM1 mRNA in the lung tissues of ALI plays the roles in ALI. The application of rhubarb and dexamethasone can decrease the expression and ameliorate the lung damage; its mechanism is possibly via the inhibition of ICAM1 mRNA expression.
文摘Purpose The warming-Yang or cooling-heat is the principle rules of traditional Chinese medicine theory. The purpose of this study is to identify the effect of warming-Yang or cooling-heat Chinese medicine prescriptions combining with Gefitinib on lung cancer H1650 cell line. Materials and Methods： The typical warming-Yang prescription, Shen-fu （SF） Injection, and cooling-heat prescription, Qing-kai-ling （QKL） Injection was combined with Gefitinib respectively to treat H1650 cells. Cell viability was measured by MTT assay and Annexin V/PI method. Epidermal growth factor receptor （EGFR） pathway proteins were detected by Western blot. Results： QKL combined with Gefitinib significantly suppressed cell proliferation and induced apoptosis in H1650 cell line compared with single drug （P 〈 0.05）. On the contrary, SF and Gefitinib had significant antagonism effects on both cell viability inhibition and apoptosis （48h： 0.85 〈 Q 〈 1.15, 72h： Q 〈 0.85）. Although higher EGFR protein level was observed in Gefitinib group, which was significantly reduced in QKL ＋ Gefitinib group, no significant differences were observed in the levels of p-EGFR （P 〉 0.05）, downstream AKT/p-AKT （P 〉 0.05）, or ERK/p-ERK （P 〉 0.05） among Gefitinib, Gefitinib ＋ SF, and Gefitinib ＋ QKL groups. Conclusion： QKL Injection may reduce the level of EGFR and then improve the resistance of H1650 cells to EGFR-TKIs, which may be the mechanism of synergism effect of QKL Injection and Gefitinib. The principle rules of warming-Yang or cooling-heat should be further elucidated, and more research are required to explore the combined effect of Chinese medicine with opposite principle and Gefitinib.
基金supported by grants from the National Natural Science Foundation of China(No.30900384)Education Bureau Foundation of Liaoning Province,China (No.2009a723)
文摘Objective Radiation-induced lung injury （RILl） is the most common, dose-limiting complication in thoracic malignancy radiotherapy. Considering its negative impact on patients and restrictions to efficacy, the mechanism of RILl was studied. Methods Wistar rats were locally irradiated with a single dose of 0, 16, and 20 Gy to the right half of the lung to establish a lung injury model. Two and six months after irradiation, the right half of the rat lung tissue was removed, and the concentrations of TGF-[31, angiotensin II, and aldosterone were determined via enzyme-linked immunosorbent assay. Results Statistical differences were observed in the expression levels of angiotensin II and aldosterone between the non-irradiation and irradiation groups. Moreover, the expression level of the angiotensin II-aldosterone system increased with increasing doses, and the difference was still observed as time progressed. Conclusions Angiotensin II-aldosterone system has an important pathophysiological function in the progression of RILI.
文摘Objective： The aim of the study was to investigate whether enhanced anti-tumor effects of endostar （ES） on radiation involved hypoxia inducible factor-la （HIF-la） and basic ~broblast growth factor （bFGF）. Methods： A549 cells were divided into four groups： control group; endostar （ES） group; radiation （RT） group; endostar plus radiation （ES ＋ RT） group. The inhibition of proliferation rates ofA549 cells was measured by cell counting kit-8 （CCK-8）. HIF-la and bFGF expression levels were checked by enzyme linked immunosorbent assay （ELISA） and reverse transcription-polymerase chain reaction （RT-PCR）. Results： The proliferation inhibition rate in the ES ＋ RT group was higher than that in other groups. On the other hand, the expressions of HIF-1α and bFGF in the ES ＋ RT group were significantly reduced compared to other groups. HIF-1α and bFGF levels were positively correlated （r = 0.80, P 〈 0.01）. Conclusion： Our results suggest that endostar could enhance anti-tumor effect of radiation by reducing expressions of HIF-1α and bFGF.
文摘Objective: To investigate the effect of Shen-Fu (SF) injection on gastrointestinal tract injury and its potential mechanism.Methods: Thirty-eight patients undergoing elective open heart surgery were assigned to Group C (control group, n = 18) and Group SF (n = 20) randomly. In Group SF, the patients received intravenous injection of SF (0.5 ml/kg) at the beginning of the surgery followed by a continuous infusion of 100 ml SF (1.0 ml/kg) solution diluted by saline at a rate of 0.004 ml · Kg-1 · min-1 with a Grasby pump. The control group was injected with normal saline in the same volume. Gastric intramucosal pH (pHi), activity of blood diamine oxidase ( DAO ), and concentrations of blood LPS and IL-6 were measured before CPB ( S0) and 1 h ( S1 ) and 2 h ( S2) after aortic declamping, respectively.Results: In Group C, pHi value was significantly lower at S1 and S2 than at S0 ( mean P <0.01) and blood DAO and concentrations of LPS and IL-6 were significantly higher at S1 and S2 than at S0 ( meanP < 0.01). In Group SF, pHi was obviously lower at S1 and S2 than at S0(P< 0.05) but LPS and IL-6 levels and DAO were higher at S, (mean P<0.05). Blood DAO and LPS level demonstrated significant negative correlations with pHi ( mean P < 0.01) while LPS concentration showed a positive correlation with blood DAO (P < 0.01) and IL-6 concentration (P < 0.05). At S1 and S2 after aortic declamping, the levels of pHi were higher in Group SF than in Group C (mean P <0.01 ) but DAO and LPS and IL-6 levels were significantly lower in Group SF than in Group C ( P <0.01).Conclusions: SF has a protective effect on gastrointestinal tract and can reduce inflammatory actions.
基金Supported by Special Project on Scientific Research into Traditional Chinese Medicine in People's Liberation Army(10ZYZ241)
文摘OBJECTIVE: To observe the effect of decoction for reinforcing lung Qi on T-lymphocytic function, interleukin-2 (IL-2) and tumor necrosis factor-α (TNF-α) in peripheral blood of patients with non-small cell lung cancer after operation with argon helium lancet in order to explore its mechanism.METHODS: A total of 76 patients suffering from non-small cell lung cancer without surgical indication were randomly divided into a treatment group treated with decoction for reinforcing lung Qi and argon helium lancet and a control group treated with argon helium lancet only to observe lymphocytic proliferation, detect the percentage of positive cells in the T-lymphocyte CD28 with flow cytometry and detect the expression of IL-2 and TNF-α in peripheral blood with enzyme linked immunosorbent assay.RESULTS: Proliferation of T-lymphocytes and expression of CD28, IL-2 and TNF-α in peripheral blood after treatment in the treatment group were more obviously strengthened than those before treatment and those in the control group (all P<0.05).CONCLUSION: The mechanism of using decoction for reinforcing lung Qi and argon helium lancet to treat lung cancer may be realized through promoting T-lymphocytic proliferation, up-regulating expression of CD28, IL-2 and TNF-α, and activating T-cells.
