The effects of four ions and eight neuroactive compounds on inducing larval settlement ofA. japonicus were assessed in the present study. All bioassays were conducted in 60 × 9mm Petri dishes, each contained 10mL...The effects of four ions and eight neuroactive compounds on inducing larval settlement ofA. japonicus were assessed in the present study. All bioassays were conducted in 60 × 9mm Petri dishes, each contained 10mL of the test solution and 10 doliolaria larvae. There were significant inductive effects of K＋ （10 mmol L-l）, NH4＋ （0.1 mmol L-l）, GABA （10-3 tool L-l）, acetylcholine （10-5 molL-l）, L-DOPA （10-SmolL-1）, norepinephrine （10-SmolL-1） and dopamine （10-TmolL-1 and 10-5 molL-1） on the settlement of sea cucumber larvae. L-DOPA and dopamine are the most efficient chemical cues to induce A.japonicus larvae to settle. The highest percentage of larval settlement was induced by 10-5 tool L-1 L-DOPA and dopamine （33% and 40%） compared to the control （7%）. However, Ca2＋, Mg2＋, choline, serotonin, and epinephrine were less effective on larval settlement at all tested concentrations. This study evaluated the stability and feasibility of chemical cues for larval settlement in different culture systems, which can be applied to improve the hatchery production of this valuable species.展开更多
基金supported by the grants from National Marine Public Welfare Research Program (201205023)the Scientific and Technical Supporting Program (2011 BAD13B03)Shandong Seed Project
文摘The effects of four ions and eight neuroactive compounds on inducing larval settlement ofA. japonicus were assessed in the present study. All bioassays were conducted in 60 × 9mm Petri dishes, each contained 10mL of the test solution and 10 doliolaria larvae. There were significant inductive effects of K＋ （10 mmol L-l）, NH4＋ （0.1 mmol L-l）, GABA （10-3 tool L-l）, acetylcholine （10-5 molL-l）, L-DOPA （10-SmolL-1）, norepinephrine （10-SmolL-1） and dopamine （10-TmolL-1 and 10-5 molL-1） on the settlement of sea cucumber larvae. L-DOPA and dopamine are the most efficient chemical cues to induce A.japonicus larvae to settle. The highest percentage of larval settlement was induced by 10-5 tool L-1 L-DOPA and dopamine （33% and 40%） compared to the control （7%）. However, Ca2＋, Mg2＋, choline, serotonin, and epinephrine were less effective on larval settlement at all tested concentrations. This study evaluated the stability and feasibility of chemical cues for larval settlement in different culture systems, which can be applied to improve the hatchery production of this valuable species.
