AIM:To evaluate the biological and clinical characteristics of miR-622 in gastric cancer. METHODS:We analyzed the expression of miR-622 in 57 pair matched gastric neoplastic and adjacent non-neoplastic tissues by quan...AIM:To evaluate the biological and clinical characteristics of miR-622 in gastric cancer. METHODS:We analyzed the expression of miR-622 in 57 pair matched gastric neoplastic and adjacent non-neoplastic tissues by quantitative real-time polymerase chain reaction. Functional analysis of miR-622 expression was assessed in vitro in gastric cancer cell lines with miR-622 precursor and inhibitor. The roles of miR-622 in tumorigenesis and tumor metastasis were analyzed using a stable miR-622 expression plasmid in nude mice. A luciferase reporter assay was used to assess the effect of miR-622 on inhibitor of growth family,member 1 (ING1) expression. RESULTS:Expression of miR-622 was down-regulated in gastric cancer. MiR-622 was found involved in differentia-tion and lymphatic metastasis in human gastric cancer. Ectopic expression of miR-622 promoted invasion,tumorigenesis and metastasis of gastric cancer cells both in vitro and in vivo. ING1 is a direct target of miR-622. CONCLUSION:These findings help clarify the molecular mechanisms involved in gastric cancer metastasis and indicate that miR-622 modulation may be a bona fide treatment of gastric cancer.展开更多
2020年初美国临床肿瘤学会(American Society of Clinical Oncology,ASCO)首次在Journal of Clinical Oncology发布了《卵巢上皮性癌胚系和体细胞肿瘤检测指南》[1]。该指南以2007—2019年间发表的6篇Meta分析、11篇随机对照试验、2篇...2020年初美国临床肿瘤学会(American Society of Clinical Oncology,ASCO)首次在Journal of Clinical Oncology发布了《卵巢上皮性癌胚系和体细胞肿瘤检测指南》[1]。该指南以2007—2019年间发表的6篇Meta分析、11篇随机对照试验、2篇观察性研究的相关文献为主要参考依据,为卵巢上皮性癌肿瘤基因突变检测相关临床应用和实施提供了指导和建议。展开更多
基金Supported by Grants from Science Foundation of Shandong Province of China (2003-23)Key Research Project from Shan-dong Science and Technology Commission, No. 2005GG3202066
文摘AIM:To evaluate the biological and clinical characteristics of miR-622 in gastric cancer. METHODS:We analyzed the expression of miR-622 in 57 pair matched gastric neoplastic and adjacent non-neoplastic tissues by quantitative real-time polymerase chain reaction. Functional analysis of miR-622 expression was assessed in vitro in gastric cancer cell lines with miR-622 precursor and inhibitor. The roles of miR-622 in tumorigenesis and tumor metastasis were analyzed using a stable miR-622 expression plasmid in nude mice. A luciferase reporter assay was used to assess the effect of miR-622 on inhibitor of growth family,member 1 (ING1) expression. RESULTS:Expression of miR-622 was down-regulated in gastric cancer. MiR-622 was found involved in differentia-tion and lymphatic metastasis in human gastric cancer. Ectopic expression of miR-622 promoted invasion,tumorigenesis and metastasis of gastric cancer cells both in vitro and in vivo. ING1 is a direct target of miR-622. CONCLUSION:These findings help clarify the molecular mechanisms involved in gastric cancer metastasis and indicate that miR-622 modulation may be a bona fide treatment of gastric cancer.
文摘2020年初美国临床肿瘤学会(American Society of Clinical Oncology,ASCO)首次在Journal of Clinical Oncology发布了《卵巢上皮性癌胚系和体细胞肿瘤检测指南》[1]。该指南以2007—2019年间发表的6篇Meta分析、11篇随机对照试验、2篇观察性研究的相关文献为主要参考依据,为卵巢上皮性癌肿瘤基因突变检测相关临床应用和实施提供了指导和建议。
