^(99m)Tc-MIBI与^(99m)Tc-PPM肺肿瘤阳性显像的对比研究

目的：对比分析99mTc-甲氧基异丁基异睛（MIBI）与99mTc-培普利欧霉素（PPM）肺肿瘤显像的特点与诊断效能。方法：对48例使用99mTc－MIBI与39例使用99Tc－PPM肿瘤阳性显像的结果通过目测观察，T／N（肿瘤与非肿瘤）比值测定，储存指数RI测定，延迟显像摄取指数DUI测定并对其结果进行对比。结果：目测观察，99mTc－MIBI显像对恶性肿瘤诊断的灵敏度为94．12％（32／34），特异性为85．71％（12／14），99mTc－PPM显像对恶性肿瘤诊断的灵敏度为90.91％（30／33），特异性为83．33％（5／6），两者之间无显著性差异（P＞0．05）；99mTc－MIBI在恶性肿瘤组早期T／N为1．36,晚期T／N为1．37，RI值为0．74DUI值为1．38，而99mTc-PPM在恶性肿瘤组分别为1．13，1．3611．77及1．64，其中两组早期T／N之间，晚期T／N之间均无显著性差异（P＞0．05），两组RI值之间有非常显著性差异（P＜0．01），两组DUI之间有显著性差异（P＜0．05）；两种显像剂在良性病变组各参数之间的差异均无显著性（P＞0.05）。结论：99mTc-L－MIBI及99mTc－PPM肺肿瘤阳性显像对肺肿瘤的诊断及鉴别其良恶性有一定价值；恶性肿瘤对两种显像剂的外排存在差异。

Reversing drug resistance in the ovarian carcinoma cell line SKOV3/mdr1 in vitro by antisense oligodeoxynucleotides

Objective To investigate the effect of multidrug resistance gene 1 (mdr1) antisense oligodeoxynucleotides (ODNs) on reversing multidrug resistance in the drug resistant ovarian carcinoma cell line SKOV3/mdr1. Methods The ovarian carcinoma cell line SKOV3 transducted with a human multidrug resistance gene (mdr1) served as the drug resistant model (SKOV3/mdr1). The mdr1 antisense ODNs was transfected into SKOV3/mdr1 cells while mediated by lipofectamine. Reverse transcription-polymerase chain reaction (RT-PCR) was used to measure the expression and the amount of the mdr1 mRNA in the cells. The positive rate and function of the mdr1 gene product P-glycoprotein (Pgp) in the mdr1 antisense ODNs treated SKOV3/mdr1 cells were determined by flow cytometry and rhodamine 123 efflux. Drug resistance in the SKOV3/mdr1 cell line was observed by MTT assay and cell colony culture. Results The mdr1 mRNA level was decreased to about 60% of that of β-actin after mdr1 antisense ODNs treatment. The Pgp positive rate of mdr1 antisense ODNs treated SKOV3/mdr1 cells decreased from 100% to 52.6% (P<0.01). The intracellular rhodamine 123 retention was increased from 9.1% to 33.8% (P<0.01). The chemoresistance to taxol decreased to 58% of SKOV3/mdr1 with mdr1 antisense ODN treatment. Compared with SKOV3/mdr1 cells in the control group, under a certain range of drug concentrations, the number of drug resistance colonies in mdr1 antisense ODNs treated SKOV3/mdr1 cells for taxol and doxorubicin decreased by 8.6±0.8 fold and 3.1±0.6 fold, respectively. Some non-specific functions during oligodeoxyncleotide treatment was also detected. Conclusion mdr1 expression in the SKOV1/mdr1 cell line was partially inhibited after mdr1 antisense ODNs treatment at the mRNA and protein level, increasing the chemotherapy sensitivity of this drug resistant ovarian carcinoma cell line.