Objective: To detect the expression of cyclinD1 and Ki67 in nasopharyngeal carcinoma (NPC) and their correlation with the biological behaviors and prognosis. Methods: 56 cases of biopsy specimens ...Objective: To detect the expression of cyclinD1 and Ki67 in nasopharyngeal carcinoma (NPC) and their correlation with the biological behaviors and prognosis. Methods: 56 cases of biopsy specimens of NPC which had been embedded with para?n in 1996 in our hospital were collected and immunostained with cyclinD1 and Ki67 monoclonal antibodies by means of the streptavidin peroxides method. The patients were followed up periodically, and then their biological behaviors and prognosis were statistically analyzed. Results: The percentage of cyclinD1 and Ki67 positive cells in the NPC specimens ranged from 0–54% and 0–31% respectively. The staining was nuclear. Of the 56 cases, 30 cases (56.6%) highly expressed cyclinD1 or Ki67 HPI and 26 cases (46.4%) lowly expressed cyclinD1, while only 16 cases (28.6%) showed Ki67 HPI (high proliferated index) and 40 cases (71.4%) showed Ki67 LPI (low proliferated index). Patients who lowly expressed cyclinD1 or highly expressed Ki67 had a higher radiosensitivity and a better prognosis. Conclusion: CyclinD1 and Ki67 immunohistochemical staining is considered to be useful, not only as an independent factor of radiosensitivity and prognosis respectively, but also as a means of determining the optimum treatment for each individual patient with NPC.展开更多
The clinical management of metastatic （stage IV） colorectal cancer （CRC） is a common challenge faced by surgeons and physicians. The last decade has seen exciting developments in the management of CRC, with signif...The clinical management of metastatic （stage IV） colorectal cancer （CRC） is a common challenge faced by surgeons and physicians. The last decade has seen exciting developments in the management of CRC, with significant improvements in prognosis for patients diagnosed with stage IV disease. Treatment options have expanded from 5-fluorouracil alone to a range of pharmaceutical and interventional therapies, improving survival, and providing a cure in selected cases. Enhanced understanding of the biologic pathways most important in colorectal carcinogenesis has led to a new generation of drugs showing promise in advanced disease. It is hoped that in the near future the treatment paradigm of metastatic CRC will be analogous to that of a chronic illness, rather than a rapidly terminal condition. This overview discusses the epidemiology of advanced CRC and currently available therapeutic options including medical, surgical, ablative and novel modalities in the management of metastatic colorectal cancer.展开更多
Objective: To study the relationship between the expressions of multidrug-resistance, 1 (mdr1) gene-coded mRNA and its product P-glycoprotein (P-gp) and biological characteristics of tumor cells in ...Objective: To study the relationship between the expressions of multidrug-resistance, 1 (mdr1) gene-coded mRNA and its product P-glycoprotein (P-gp) and biological characteristics of tumor cells in patients with previously untreated primary pancreatic cancer (PC) for guiding signi?cance to the clinical treatment. Methods: Expression of mdr1 mRNA and P-gp on para?n embedded sections was detected by in situ polymerase chain reaction (ISPCR) and immunohistochemistry correspondingly from 150 cases of normal and abnormal pancreatic tissues including 97, 32 and 21 cases of pancreatic cancer, pancreatitis and normal pancreatic tissues respectively. Results: Distributions of positive staining in mdr1 mRNA and P-gp were mainly found on the apical plasma membranes and in cytoplasms of endothelial duct cells in tumor and normal tissues. The positive staining rates of expression of the mdr1 mRNA and P-gp detected in all pancreatic tumors were signi?cantly higher than that in pancreatitis and normal tissues correspondingly (P <0.05). Moreover, higher expressions of mdr1 mRNA and P-gp in tumor cells were correlated with some biological characteristics of PC, such as the degree of di?erentiation, aggressiveness and TNM stage of tumors (P <0.05). However, there was no correlation between the rate of expression of mdr1 mRNA and P-gp and some clinical ?ndings including age, sex, location and tumor size. Conclusion: The expression of mdr1 gene was associated with “natural” multi-drug resistance in PC. There was an important guiding signi?cance between the detection of expression of mdr1 gene and prediction of the sensitivity to chemotherapy of PC. Meanwhile, it probably could be used as one of profoundly parameters to assess the degrees of di?erentiation and prognosis in PC.展开更多
文摘Objective: To detect the expression of cyclinD1 and Ki67 in nasopharyngeal carcinoma (NPC) and their correlation with the biological behaviors and prognosis. Methods: 56 cases of biopsy specimens of NPC which had been embedded with para?n in 1996 in our hospital were collected and immunostained with cyclinD1 and Ki67 monoclonal antibodies by means of the streptavidin peroxides method. The patients were followed up periodically, and then their biological behaviors and prognosis were statistically analyzed. Results: The percentage of cyclinD1 and Ki67 positive cells in the NPC specimens ranged from 0–54% and 0–31% respectively. The staining was nuclear. Of the 56 cases, 30 cases (56.6%) highly expressed cyclinD1 or Ki67 HPI and 26 cases (46.4%) lowly expressed cyclinD1, while only 16 cases (28.6%) showed Ki67 HPI (high proliferated index) and 40 cases (71.4%) showed Ki67 LPI (low proliferated index). Patients who lowly expressed cyclinD1 or highly expressed Ki67 had a higher radiosensitivity and a better prognosis. Conclusion: CyclinD1 and Ki67 immunohistochemical staining is considered to be useful, not only as an independent factor of radiosensitivity and prognosis respectively, but also as a means of determining the optimum treatment for each individual patient with NPC.
文摘The clinical management of metastatic （stage IV） colorectal cancer （CRC） is a common challenge faced by surgeons and physicians. The last decade has seen exciting developments in the management of CRC, with significant improvements in prognosis for patients diagnosed with stage IV disease. Treatment options have expanded from 5-fluorouracil alone to a range of pharmaceutical and interventional therapies, improving survival, and providing a cure in selected cases. Enhanced understanding of the biologic pathways most important in colorectal carcinogenesis has led to a new generation of drugs showing promise in advanced disease. It is hoped that in the near future the treatment paradigm of metastatic CRC will be analogous to that of a chronic illness, rather than a rapidly terminal condition. This overview discusses the epidemiology of advanced CRC and currently available therapeutic options including medical, surgical, ablative and novel modalities in the management of metastatic colorectal cancer.
文摘Objective: To study the relationship between the expressions of multidrug-resistance, 1 (mdr1) gene-coded mRNA and its product P-glycoprotein (P-gp) and biological characteristics of tumor cells in patients with previously untreated primary pancreatic cancer (PC) for guiding signi?cance to the clinical treatment. Methods: Expression of mdr1 mRNA and P-gp on para?n embedded sections was detected by in situ polymerase chain reaction (ISPCR) and immunohistochemistry correspondingly from 150 cases of normal and abnormal pancreatic tissues including 97, 32 and 21 cases of pancreatic cancer, pancreatitis and normal pancreatic tissues respectively. Results: Distributions of positive staining in mdr1 mRNA and P-gp were mainly found on the apical plasma membranes and in cytoplasms of endothelial duct cells in tumor and normal tissues. The positive staining rates of expression of the mdr1 mRNA and P-gp detected in all pancreatic tumors were signi?cantly higher than that in pancreatitis and normal tissues correspondingly (P <0.05). Moreover, higher expressions of mdr1 mRNA and P-gp in tumor cells were correlated with some biological characteristics of PC, such as the degree of di?erentiation, aggressiveness and TNM stage of tumors (P <0.05). However, there was no correlation between the rate of expression of mdr1 mRNA and P-gp and some clinical ?ndings including age, sex, location and tumor size. Conclusion: The expression of mdr1 gene was associated with “natural” multi-drug resistance in PC. There was an important guiding signi?cance between the detection of expression of mdr1 gene and prediction of the sensitivity to chemotherapy of PC. Meanwhile, it probably could be used as one of profoundly parameters to assess the degrees of di?erentiation and prognosis in PC.
