卵巢癌组织中DNMT1、STAT2表达变化及临床意义

目的:探讨卵巢癌组织中DNA甲基转移酶-1(DNMT1)、信号转导及转录活化因子2(STAT2)表达及临床意义。方法:选取2017年7月-2021年7月本院行手术切除卵巢癌患者80例为卵巢癌组,同期因子宫肌瘤、子宫腺肌症行全子宫+双附件切除术患者80例为对照组,应用免疫组化法测定卵巢癌组织与正常卵巢组织中DNMT1、STAT2表达,分析卵巢癌组织中DNMT1、STAT2表达相关性及与临床病理参数的关系,以Kaplan-Meier生存曲线分析癌组织中DNMT1、STAT2表达与患者1年生存率的关系,Cox分析卵巢癌患者预后影响因素。结果:卵巢癌组DNMT1(83.8%)、STAT2(67.5%)表达阳性率均高于对照组(21.3%、8.8%)(P<0.05);FIGOⅢ+Ⅳ期、肿瘤分化程度低分化、有淋巴结转移、有腹水卵巢癌患者癌组织中DNMT1、STAT2表达阳性率高于Ⅰ+Ⅱ期、中、高分化、无淋巴结转移、无腹水患者(P<0.05);Spearman相关性分析显示,卵巢癌组织中DNMT1、STAT2表达呈显著正相关(P<0.05);KaplanMeier生存曲线分析显示,卵巢癌组DNMT1表达阳性与阴性患者1年总生存率(72.3%、92.3%)无差异(P>0.05),STAT2表达阴性患者1年总生存率(92.0%)高于阳性患者(67.9%)(P<0.05);行Cox分析发现,FIGO分期、肿瘤分化程度、淋巴结转移及癌组织中STAT2高表达均为患者1年预后死亡的影响因素(P<0.05)。结论:卵巢癌组织中DNMT1、STAT2表达显著增高,两者可能协同参与了卵巢癌发生发展及转移,且与患者1年预后有一定相关性。

Association of ezrin expression in intestinal and diffuse gastric carcinoma with clinicopathological parameters and tumor type

AIM： To investigate the correlation between ezrin expression and types of gastric carcinoma and clinicopathological variables.METHODS： We examined ezrin protein expression in 75 gastric carcinoma （53 intestinal types of adenocarcinoma, 22 diffuse types of carcinoma） tissues by immunohistochemistry. The results were compared with clinicopathological parameters such as tumor type, grade of tumor, clinical stage, presence of metastatic lymph node, and depth of invasion. RESULTS： Ezrin immunostaining was positive in 43 cases （81.1%） of intestinal type and in 9 （40.9%） cases of diffuse type adenocarcinomas （P 〈 0.001）. In gastric carcinomas, the expression of ezrin protein correlated with the status of H py/ori and survival. There was no correlation between expression of ezrin with TNM stage and histological grade of gastric carcinomas （P 〉 0.05）. CONCLUSION： The low expression of ezrin implicates the loss of adhesion in diffuse carcinomas. Furthermore, overexpression of ezrin in carcinomas with H pylori infection may be a genuine specific pathway in which Hpylori may cause/initiate gastric carcinoma.