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亚甲基四氢叶酸脱氢酶2与肿瘤表型关系的研究进展
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作者 黄美慧 许圳南 +3 位作者 薛家健 杜则澎 许海雄 刘明发 《癌变.畸变.突变》 CAS 2022年第5期400-403,共4页
亚甲基四氢叶酸脱氢酶2(MTHFD2)是一种具有亚甲基四氢叶酸脱氢酶和甲基四氢叶酸环化水解酶活性的线粒体酶,是线粒体一碳代谢途径的主要酶之一,参与一碳代谢、核苷酸合成、DNA复制和修复以及核糖核酸代谢等过程。近年来的研究显示,MTHFD... 亚甲基四氢叶酸脱氢酶2(MTHFD2)是一种具有亚甲基四氢叶酸脱氢酶和甲基四氢叶酸环化水解酶活性的线粒体酶,是线粒体一碳代谢途径的主要酶之一,参与一碳代谢、核苷酸合成、DNA复制和修复以及核糖核酸代谢等过程。近年来的研究显示,MTHFD2在多种恶性肿瘤中异常高表达,与患者的不良预后密切相关,推测其在参与恶性肿瘤的发生发展过程中可能发挥重要的生物学功能。本文就MTHFD2在肿瘤细胞增殖、迁移侵袭、死亡及其临床意义等方面的相关研究进展进行综述。 展开更多
关键词 亚甲基四氢叶酸脱氢酶2 一碳代谢 恶性肿瘤 肿瘤表型
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胃癌中FTO表达与TGF-β表达、肿瘤免疫表型的关系及临床意义
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作者 赖唤 胡楠 +6 位作者 吴学雨 周康杰 蒋薇薇 张淼 洪义东 张竞舟 吴风雷 《现代肿瘤医学》 CAS 北大核心 2023年第8期1461-1467,共7页
目的:分析胃癌组织中FTO表达与TGF-β表达及肿瘤免疫表型的关系及临床意义。方法:采用TIMER数据库分析胃癌组织中FTO和TGF-βmRNA的表达情况。采用GEPIA数据库评估FTO及TGF-βmRNA在胃癌中表达的相关性。利用TIMER数据库分析FTO对胃癌... 目的:分析胃癌组织中FTO表达与TGF-β表达及肿瘤免疫表型的关系及临床意义。方法:采用TIMER数据库分析胃癌组织中FTO和TGF-βmRNA的表达情况。采用GEPIA数据库评估FTO及TGF-βmRNA在胃癌中表达的相关性。利用TIMER数据库分析FTO对胃癌免疫细胞浸润水平的影响。采用免疫组织法检测胃FTO、TGF-β、CD8+T细胞的表达,分析FTO与TGF-β、肿瘤免疫表型的相关性,及其与临床病理特征的关系。结果:胃癌组织中FTO、TGF-β阳性率皆高于癌旁组织(P<0.05);分析三种免疫表型在胃癌中的表达情况,发现在胃癌组织中以免疫豁免型为主(P<0.05);在胃癌中,FTO表达水平与TGF-β表达水平、免疫细胞浸润及免疫豁免型呈显著正相关(P<0.05);FTO表达与淋巴结转移、TNM分期及分化程度密切相关(P<0.05)。结论:FTO在胃癌中高表达,可影响TGF-β表达水平、免疫细胞浸润水平及肿瘤免疫表型。 展开更多
关键词 胃癌 FTO TGF-Β 免疫细胞浸润 肿瘤免疫表型
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三维细胞培养与肿瘤细胞恶性表型研究 被引量:2
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作者 何平 张学 赵晓航 《生命的化学》 CAS CSCD 北大核心 2005年第3期252-255,共4页
了解肿瘤细胞与微环境的相互作用,对研究肿瘤的发生、发展及抗癌药物的筛选具有重要意义。三维细胞培养技术近年被用于研究肿瘤细胞恶性表型,与传统二维细胞培养相比,它可以模拟体内细胞生长的微环境,是研究肿瘤细胞恶性表型、细胞与细... 了解肿瘤细胞与微环境的相互作用,对研究肿瘤的发生、发展及抗癌药物的筛选具有重要意义。三维细胞培养技术近年被用于研究肿瘤细胞恶性表型,与传统二维细胞培养相比,它可以模拟体内细胞生长的微环境,是研究肿瘤细胞恶性表型、细胞与细胞外基质信号传递的有力工具。 展开更多
关键词 三维细胞培养 肿瘤细胞恶性表型
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卵巢成熟性囊性畸胎瘤恶变为血管肉瘤1例并文献复习
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作者 李莹莹 李小华 +2 位作者 门楠 杨向君 冬国友 《诊断病理学杂志》 2024年第8期765-767,共3页
目的探讨卵巢成熟性囊性畸胎瘤(MOCT)恶变为血管肉瘤的临床病理特征、诊断、鉴别诊断及其治疗。方法收集1例MOCT恶变为血管肉瘤患者的临床病理资料,并对相关文献进行复习。结果患者66岁,间断下腹痛3个月,发现盆腔包块2+月,影像学考虑畸... 目的探讨卵巢成熟性囊性畸胎瘤(MOCT)恶变为血管肉瘤的临床病理特征、诊断、鉴别诊断及其治疗。方法收集1例MOCT恶变为血管肉瘤患者的临床病理资料,并对相关文献进行复习。结果患者66岁,间断下腹痛3个月,发现盆腔包块2+月,影像学考虑畸胎瘤可能。肿瘤系列提示CA-125、CA-199均高于正常。专科查体:盆腔包块上界达脐平,质偏硬。镜下肿瘤由空泡状的圆形、多边形细胞,类似脂肪母细胞或泡沫样细胞构成,细胞核圆形、卵圆,核仁清楚,核分裂象多见,呈巢状、片状分布,或呈裂隙状、微小囊状及迷路样腔隙,大部分腔隙内见红细胞。免疫表型:CD31广泛强阳性,Vimentin呈阳性,CD34、CK、CK7、EMA均阴性,Ki-67指数30%+。结论MOCT来源的血管肉瘤十分罕见,预后很差,对于绝经后妇女且肿瘤最大径>10 cm的要尽早行手术完整切除且尽量避免肿瘤破裂。 展开更多
关键词 成熟性囊性畸胎瘤恶变 血管肉瘤 肿瘤系列 免疫表型
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星形细胞肿瘤微血管组织芯片的构建和微血管壁组成细胞研究 被引量:3
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作者 王清良 卞修武 +1 位作者 章容 蒋雪峰 《临床与实验病理学杂志》 CAS CSCD 北大核心 2006年第4期440-443,共4页
目的初步探讨人脑星形细胞肿瘤中新生微血管的管壁组成成分与微血管构筑异质性(tumor microvascular architecturphenotype heterogeneity,T-MAPH)之间的关系。方法收集45例人脑星形细胞肿瘤标本,选择其新生微血管形态不同的“热点区域... 目的初步探讨人脑星形细胞肿瘤中新生微血管的管壁组成成分与微血管构筑异质性(tumor microvascular architecturphenotype heterogeneity,T-MAPH)之间的关系。方法收集45例人脑星形细胞肿瘤标本,选择其新生微血管形态不同的“热点区域”制备组织芯片;在此基础上采用免疫组织化学染色技术分别进行GFAP、CD34和α-SMA标记,观察微血管管壁组成细胞的免疫表型与其不同形态特征之间的关系。结果成功构建了含幼稚微血管、厚壁微血管、肾小球样微血管及薄壁、蛇行状微血管等各种形态特征的153点组织芯片;各种形态的微血管中均可检测到呈单层排列、位于微血管最内层的CD34阳性的内皮细胞;α-SMA阳性反应可见于内皮细胞外相当于周细胞处,其阳性表达范围及数目在不同形态的微血管中呈现多样性:幼稚微血管仅见少量表达,而在厚壁和“肾小球样”微血管中α-SMA阳性细胞多呈活跃增生的单层或多层。结论星形细胞瘤微血管形态构筑的多样性(异质性)是由内皮细胞和α-SMA阳性周细胞共同参与形成的,而后者的作用可能更为突出和重要。 展开更多
关键词 肿瘤 星形细胞瘤 血管生成 周细胞 肿瘤微血管构筑表型 组织芯片
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Hippo通路与乳腺癌发生发展的作用研究进展与应用前景 被引量:2
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作者 李欣芳 地里呼玛尔·吐鲁洪(综述) 王少华(审校) 《临床肿瘤学杂志》 CAS 2022年第10期945-951,共7页
在发病率逐年增高和死亡率居高不下的全球大背景下,乳腺癌一直是科研领域的研究热点。Hippo通路广泛涉及了从乳腺正常发育至乳腺癌发生发展、侵袭转移及耐药等一系列过程。该综述以乳腺癌总人群发病特征及Hippo通路发展历程为背景,围绕... 在发病率逐年增高和死亡率居高不下的全球大背景下,乳腺癌一直是科研领域的研究热点。Hippo通路广泛涉及了从乳腺正常发育至乳腺癌发生发展、侵袭转移及耐药等一系列过程。该综述以乳腺癌总人群发病特征及Hippo通路发展历程为背景,围绕肿瘤干细胞、上皮-间质转化及凋亡三方面介绍了Hippo通路对乳腺癌肿瘤表型的调控,最后从临床应用前景上做出展望,介绍了Hippo通路成分对乳腺癌患者的预后作用及可能提供的药物治疗靶点。 展开更多
关键词 乳腺癌 Hippo通路 肿瘤表型 预后 治疗靶点
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胃癌中TGF-β、PD-L1表达与肿瘤免疫表型的关系及其临床意义 被引量:5
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作者 张竞舟 胡楠 +4 位作者 汤佳琦 洪义东 卞保祥 宋子琰 吴风雷 《中国现代应用药学》 CAS CSCD 北大核心 2021年第9期1025-1030,共6页
目的检测TGF-β、PD-L1在胃癌患者中的表达情况,并探讨两者表达与肿瘤免疫表型的关系及其临床意义。方法收集南京医科大学连云港临床医学院2017—2019年84例行胃癌切除术患者的肿瘤石蜡组织标本及其对应的临床病例特征。采用免疫组织法... 目的检测TGF-β、PD-L1在胃癌患者中的表达情况,并探讨两者表达与肿瘤免疫表型的关系及其临床意义。方法收集南京医科大学连云港临床医学院2017—2019年84例行胃癌切除术患者的肿瘤石蜡组织标本及其对应的临床病例特征。采用免疫组织法检测其中PD-L1、TGF-β和CD8+T细胞的表达,采用GraphPad Prism7和SPSS 21.0统计软件分析TGF-β、PD-L1表达与肿瘤免疫表型的关系及其临床意义。结果 TGF-β在胃癌中的阳性表达率为76.2%,PD-L1在胃癌中的阳性表达率为79.7%。免疫炎症型、免疫豁免型、免疫沙漠型3种免疫表型在胃癌中的表达率分别为33.3%,51.2%及15.5%。胃癌中3种免疫表型之间TGF-β表达水平差异具有统计学意义(P<0.05)。其中,胃癌中免疫豁免型与TGF-β表达水平呈显著正相关(P<0.05)。胃癌中3种免疫表型之间PD-L1表达水平差异无统计学意义。胃癌中TGF-β表达和PD-L1表达正相关(P<0.05)。胃癌中TGF-β表达与淋巴结转移(P<0.05)、TNM分期(P<0.05)密切相关。胃癌中PD-L1表达与浸润程度(P<0.05)、淋巴结转移(P<0.05)、TNM分期(P<0.05)密切相关。免疫豁免型与肿瘤直径密切相关(P<0.05)。结论胃癌中TGF-β和PD-L1均有表达,且胃癌中TGF-β表达与PD-L1表达呈显著正相关。胃癌中TGF-β和胃癌肿瘤免疫表型显著相关,TGF-β高表达患者多为免疫豁免型。而胃癌中PD-L1表达与胃癌肿瘤免疫表型无显著相关性。胃癌中TGF-β、PD-L1表达和肿瘤免疫表型与部分临床病理特征(肿瘤侵袭程度、淋巴结转移、TNM分期、肿瘤直径等)密切相关。 展开更多
关键词 胃癌 免疫卡控点阻断治疗(ICB) 肿瘤免疫表型 TGF-Β PD-L1
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Tumor differentiation phenotype in gastric differentiated-type tumors and its relation to tumor invasion and genetic alterations 被引量:9
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作者 Kimiyasu Yamazaki Yusuke Tajima +7 位作者 Reiko Makino Nobukazu Nishino Shigeo Aoki Masanori Kato Masaaki Sakamoto Koji Morohara Tsutomu Kaetsu Mitsuo Kusano 《World Journal of Gastroenterology》 SCIE CAS CSCD 2006年第24期3803-3809,共7页
AIM: To clarify the relations between tumor differentiation phenotype and tumor invasion or genetic alterations in gastric differentiated-type tumors. METHODS: We examined the tumor differentiation phenotype, the pr... AIM: To clarify the relations between tumor differentiation phenotype and tumor invasion or genetic alterations in gastric differentiated-type tumors. METHODS: We examined the tumor differentiation phenotype, the presence of mutations in APC and p53, and the microsatellite instability (MSI) status in 48 gastric adenomas and 171 differentiated-type carcinomas, The tumor differentiation phenotype was determined by examining the expression of human gastric mucin (HGM), NUC6, MUC2 and CD10, The tumors were then classified into gastric- (G-), gastric and intestinal mixed (GI-), or intestinal- (I-) phenotypes, according to the immunopositivity of the above markers, The presence of mutations in APC and p53 and the MSI status were also investigated in all the tumors, RESULTS: Gastric adenomas were significantly associated with CDIO expression, I-phenotype tumors and the presence of APC mutations, compared with carcinomas (66.7% vs 25.1%, P 〈 0.0001; 56.3% vs 14.6%, P 〈 0.0001; 39.6% vs 14.0%, P 〈 0.0001, respectively) and inversely associated with expressions of HGM and MUC6 and the presence of p53 mutations (10.4% vs 62.6%, P 〈 0.0001; 39.6% vs 64.3%, P = 0.003; 2.0% vs 26.3%, P = 0.001, respectively). The frequency of APC mutations was significantly higher in HGM-negative tumors, MUC6-negative tumors, CD10-positive tumors and I-phenotype tumors than in HGM-positive tumors, MUC6- positive tumors, CD10-negative tumors and G-phenotype tumors (32.7% vs 7.1%, P 〈 0.0001; 27.8% vs 14.0%, P = 0.0182; 37.3% vs 10.4%, P 〈 0.0001; and 38.5% vs 9.5%, P = 0.0017, respectively). The frequency of MSI was significantly higher in MUC6-positive tumors, CD10- negative tumors and G-phenotype tumors than in MUC6- negative tumors, CD10-positive tumors and I-phenotype tumors (24.8% vs 6.7%, P = 0.0009; 22.2% vs 8.0%, P = 0.0143; and 28.6% vs 9.6%, P = 0.0353, respectively). CONCLUSION: The tumor differentiation phenotype is closely related to tumor invasion and genetic alterations in gastric differentiated-type tumors. 展开更多
关键词 Gastric carcinoma Tumor differentiation phe-notype APC p53 Microsatellite instability
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HSP27在食管鳞状细胞癌KYSE150细胞中的功能
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作者 张潇 刘涛 +5 位作者 郑树涛 刘清 申铜雪 马蓉 韩秀娟 卢晓梅 《新疆医科大学学报》 CAS 2019年第4期431-436,共6页
目的研究热休克蛋白27(HSP27)对食管鳞状细胞癌(ESCC)细胞迁移、侵袭、增殖的作用。方法用Western blot技术检测5种食管癌细胞系(KYSE30、KYSE150、KYSE510、TE-1、Eca9706)中HSP27的本底表达情况。用慢病毒转染HSP27的短发夹RNA(short ... 目的研究热休克蛋白27(HSP27)对食管鳞状细胞癌(ESCC)细胞迁移、侵袭、增殖的作用。方法用Western blot技术检测5种食管癌细胞系(KYSE30、KYSE150、KYSE510、TE-1、Eca9706)中HSP27的本底表达情况。用慢病毒转染HSP27的短发夹RNA(short hairpin RNA,shRNA)至KYSE150细胞系,实验共分5组:Empty vector组(未转染的KYSE150细胞),Negative control组(转染随机序列),3组实验组(分别转染shRNA-HSP27-1、shRNA-HSP27-2、shRNA-HSP27-3)。用噻唑蓝比色法(MTT)、Transwell侵袭实验以及细胞划痕实验检测HSP27不同表达水平对食管鳞状细胞癌细胞增殖、侵袭及迁移能力的影响。结果 5种食管癌细胞系中HSP27本底表达量由高到低分别是:KYSE150、KYSE510、KYSE30、Eca9706、TE-1。与Empty vector组和Negative control组相比,HSP27敲低的实验组中食管鳞状细胞癌细胞的增殖、侵袭、迁移能力明显受抑制(P<0.05),差异有统计学意义。结论 HSP27在食管鳞状细胞癌中对肿瘤细胞的增殖、侵袭、迁移能力有促进作用,研究结果为食管癌的临床分子靶向治疗提供了新的研究方向。 展开更多
关键词 HSP27 食管鳞状细胞癌 肿瘤恶性表型
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Gene expression profiling:Canonical molecular changes and clinicopathological features in sporadic colorectal cancers 被引量:36
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作者 Jin Cheon Kim Seon Young Kim +4 位作者 Seon Ae Roh Dong-Hyung Cho Dae Dong Kim Jeong Hyun Kim Yong Sung Kim 《World Journal of Gastroenterology》 SCIE CAS CSCD 2008年第43期6662-6672,共11页
AIM: To investigate alternative or subordinate pathways involved in colorectal tumorigenesis and tumor growth, possibly determining at-risk populations and predicting responses to treatment. METHODS: Using microarra... AIM: To investigate alternative or subordinate pathways involved in colorectal tumorigenesis and tumor growth, possibly determining at-risk populations and predicting responses to treatment. METHODS: Using microarray gene-expression analysis, we analyzed patterns of gene expression relative to canonical molecular changes and clinicopathological features in 84 sporadic colorectal cancer patients, standardized by tumor location. Subsets of differentially expressed genes were confirmed by real-time reverse-transcript polymerase chain reaction (RT-PCR). RESULTS: The largest number of genes identified as being differentially expressed was by tumor location, and the next largest number by lymphovascular or neural invasion of tumor cells and by mismatch repair (NMR) defects. Amongst biological processes, the immune response was significantly implicated in entire molecular changes observed during colorectal tumorigenesis (P 〈 0.001). Amongst 47 differentially expressed genes, seven (PISD, NIBP, BAI2, STOML1, MRPL21, MRPL16, and MKKS) were newly found to correlate with tumorigenesis and tumor growth. Most location-associated molecular changes had distinct effects on gene expression, but the effects of the latter were sometimes contradictory. CONCLUSION: We show that several differentially expressed genes were associated with canonical molecular changes in sporadic colorectal cancers, possibly constituting alternative or subordinate pathways of tumorigenesis. As tumor location was the dominant factor influencing differential gene expression, location-specific analysis may identify location-associated pathways and enhance the accuracy of class prediction. 展开更多
关键词 Colorectal adenocarcinomas SPORADIC Gene expression PROFILING TUMORIGENESIS
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Effects of“Moxibustion Serum”on Proliferation and Phenotypes of Tumor Infiltrating Lymphocytes 被引量:4
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作者 陈云飞 赵粹英 +3 位作者 陈汉平 秦慧莲 方舫 王友京 《Journal of Traditional Chinese Medicine》 SCIE CAS CSCD 2003年第3期225-229,共5页
Tumor infiltrating lymphocytes (TIL) were cultured with “moxibustion serum”(MS), and the results were examined by flow cytometry. The results indicated that MS could enhance the proliferation of TIL,accelerate it to... Tumor infiltrating lymphocytes (TIL) were cultured with “moxibustion serum”(MS), and the results were examined by flow cytometry. The results indicated that MS could enhance the proliferation of TIL,accelerate it to reach the exponential growth phase, and assist recombinant interleukin 2 (rIL-2) to enhance successively the percentage of CD3^+ positive cells, maintain the number of CD4^+ positive T cells, promote greatly the percentage of CD8^+ positive T cells among TILs, and reverse the CD4^+/CD8^+ ratio. Such cooperative effects rely on relative specificity of acupoints. It is suggested that MS is beneficial to the growth of TIL both in the aspects of proliferation and phenotypes. 展开更多
关键词 ARTEMISIA Moxibustion Animals Antigens CD3 Blood CD4-CD8 Ratio Cell Division Culture Media Conditioned Drugs Chinese Herbal Female INTERLEUKIN-2 Lymphocytes Tumor-Infiltrating MICE Mice Inbred C57BL Phenotype Recombinant Proteins Thymus Neoplasms Tumor Cells Cultured
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Significance of regenerating islet-derived type Ⅳ gene expression in gastroenterological cancers 被引量:6
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作者 Masakatsu Numata Takashi Oshima 《World Journal of Gastroenterology》 SCIE CAS CSCD 2012年第27期3502-3510,共9页
The regenerating islet-derived members (Reg), a group of small secretory proteins, which are involved in cell proliferation or differentiation in digestive organs, are upregulated in several gastrointestinal cancers... The regenerating islet-derived members (Reg), a group of small secretory proteins, which are involved in cell proliferation or differentiation in digestive organs, are upregulated in several gastrointestinal cancers, functioning as trophic or antiapoptotic factors. Regenerat- ing islet-derived type Ⅳ (RegⅣ), a member of the Reg gene family, has been reported to be overexpressed in gastroenterological cancers. RegIV overexpression in tumor cells has been associated with carcinogen- esis, cell growth, survival and resistance to apoptosis. Cancer tissue expressing RegIV is generally associated with more malignant characteristics than that with- out such expression, and RegⅣ is considered a novel prognostic factor as well as diagnostic marker in some gastroenterological cancers. We previously investigated the expression levels of RegⅣ mRNA of 202 surgical colorectal cancer specimens with quantitative real-time reverse-transcriptase polymerase chain reaction and reported that a higher level of RegⅣ gene expression was a significant independent predictor of colorec- tal cancer. The biologic functions of RegⅣ protein in cancer tissue, associated with carcinogenesis, anti- apoptosis and invasiveness, are being elucidated by molecular investigations using transfection techniques or neutralizing antibodies of RegIV, and the feasibility of antibody therapy targeting RegIV is being assessed. These studies may lead to novel therapeutic strate- gies for gastroenterological cancers expressing RegⅣ. This review article summarizes the current information related to biological functions as well as clinical impor- tance of RegⅣ gene to clarify the significance of Reg~ expression in gastroenterological cancers. 展开更多
关键词 Regenerating islet-derived type protein Gastrointestinal neoplasms Prognosis Epidermal growthfactor receptor/protein kinase B
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Gene expression and MR diffusion-weighted imaging after chemoembolization in rabbit liver VX-2 tumor model 被引量:5
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作者 You-Hong Yuan En-Hua Xiao +6 位作者 Jian-Bin Liu Zhong He Ke Jin Cong Ma Jun Xiang Jian-Hua Xiao Wei-Jian Chen 《World Journal of Gastroenterology》 SCIE CAS CSCD 2008年第36期5557-5563,共7页
AIM: To investigate the dynamic characteristics and the correlation between PCNA, Bax, nm23, E-cadherin expression and apparent diffusion coefficient (ADC) on MR diffusion-weighted imaging (DWI) after chemoembolizatio... AIM: To investigate the dynamic characteristics and the correlation between PCNA, Bax, nm23, E-cadherin expression and apparent diffusion coefficient (ADC) on MR diffusion-weighted imaging (DWI) after chemoembolization in rabbit liver VX-2 tumor model. METHODS: Forty New Zealand rabbit liver VX-2 tumor models were included in the study. DWI was carried out periodically after chemoembolization. All VX-2 tumor samples in each group were examined by histopathology and Strept Avidin-Biotin Complex (SABC) immunohistochemical staining. RESULTS: The PCNA expression index in VX-2 tumors was higher than in the normal parenchyma around the tumor (P < 0.001). Nm23, Bax or E-caderin expression index in VX-2 tumors were lower than in the normal parenchyma around the tumor (all P < 0.001). PCNAand nm23 expression in the VX-2 tumor periphery first increased and then decreased (P < 0.001 and P = 0.03, respectively), while the expression of Bax and E-cadherin before and after chemoembolization was insignificant. When b-value was 100 s/mm2, there was a linear correlation between PCNA expression and ADC in the area of VX-2 tumor periphery (P < 0.001), and PCNA expression in VX-2 tumor periphery influenced the ADC. CONCLUSION: The potential of VX-2 tumor infiltrating and metastasizing decreases, while its ability to proliferate increases for a short time after chemoembolization. To some degree, the ADC value indirectly reflects the proliferation of VX-2 tumor cells. 展开更多
关键词 Rabbit liver VX-2 tumor CHEMOEMBOLIZATION Diffusion-weighted imaging Gene expression
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Meta-Analysis of the Association between Mir-196a-2 Polymorphism and Cancer Susceptibility 被引量:4
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作者 Huan Zhang Yu-liang Su +1 位作者 Herbert Yu Bi-yun Qian 《Clinical oncology and cancer researeh》 CAS CSCD 2012年第1期63-72,共10页
Objective MicroRNA plays a vital role in gene expression, and microRNA dysregulation is involved in carcinogenesis. The miR- 196a-2 polymorphism rs11614913 is reportedly associated with cancer susceptibility. This met... Objective MicroRNA plays a vital role in gene expression, and microRNA dysregulation is involved in carcinogenesis. The miR- 196a-2 polymorphism rs11614913 is reportedly associated with cancer susceptibility. This meta-analysis was performed to assess the overall association of miR-196a-2 with cancer risk. Methods A total of 27 independent case-control studies involving 10,435 cases and 12,075 controls were analyzed for the rs11614913 polymorphism. Results A significant association was found between rs11614913 polymorphism and cancer risk in four genetic models (CT vs. TT, OR-1.15, 95%CI=1.05-1.27; CC vs. TT, OR=1.23, 95%CI=1.08-1.39; Dominant model, OR=1.17, 95%CI=1.06-1.30; Additive model, OR-1.08, 95%CI=1.01-1.14). In the subgroup analysis of different tumor types, the C allele was associated with increased risk of lung, breast, and colorectat cancer, but not with liver, gastric, or esophageal cancer. In the subgroup analysis by ethnicity, a significantly increased risk of cancer was found among Asians in all genetic models, but no associations were found in the Caucasian subgroup. Conclusions The meta-analysis demonstrated that the miR-196a-2 polymorphism is associated with cancer susceptibility, especially lung cancer, colorectal cancer, and breast cancer among Asian populations. 展开更多
关键词 MIRN196 microRNA human POLYMORPHISM NEOPLASMS META-ANALYSIS
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Expressions of MVD, VEGF, Ki67 in Residual Prostate Cancer after Cryoablation 被引量:2
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作者 Yong LI Zhi GUO +1 位作者 Yan-ping HAN Xiu-ying GUO 《Clinical oncology and cancer researeh》 CAS CSCD 2011年第1期27-32,共6页
OBJECTIVE To analyze the effects of cryoablation on the mice bearing Rm-I prostate cancer through detecting tumor angiogenesis and cancer cell proliferation in the mice after cryoablation, and to explore the effects o... OBJECTIVE To analyze the effects of cryoablation on the mice bearing Rm-I prostate cancer through detecting tumor angiogenesis and cancer cell proliferation in the mice after cryoablation, and to explore the effects of cryoablation on vascular endothelium growth factor (VEGF), Ki67 protein expression and microvessel density (MVD) in the mice bearing prostate cancer. METHODS Sixty Rm-1 mouse models of prostate cancer were established. Experimental mice were randomized into 2 groups: the cryoablation group (n = 30) and the control group (n = 30). After file therap)4 tumor tissues of the mice in group A and B were obtained at day 0 (without cryoablation), 1st, 3rd, 5th, 7th, 14th day, respectivelj6 after cryoablation, and the expressions of MVD, VEGF and Ki67 proteins were detected at the same time points. RESULTS The expressions of MVD, VEGF and Ki67 proteins in group A were decreased. The lowest values of the factors were detected on the 3rd day after cryoablation, and increased slowly after that. The expressions of MVD, VEGF and Ki67 proteins in the control group were not changed. Significant changes of the expressions of MVD, VEGF and Ki67 proteins in the group A were found at different time points. Correlation analysis suggested a positive correlation between the expressions of VEGF and MVD proteins (r = 0.8793), a positive correlation between the expressions of Ki67 and MVD proteins (r = 0.7614), and a positive correlation between the expressions of VEGF and ki67 proteins (r = 0.6921). CONCLUSION After argon-helium cryoablation treatment for the mice bearing prostate cancer, the expressions of MVD, VEGF and Ki67 proteins in local tumor were reduced on the 1st day. The lowest values of the factors were detected on the 3rd day after cryoablation, and then increased after that. Cryoablation combined with other modalities of treatment may effectively improve the treatment effects of cryoablation for prostate cancer. 展开更多
关键词 CRYOABLATION prostate cancer MVD VEGF Ki67.
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肿瘤血管多样性的形成及其研究意义 被引量:6
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作者 赵文静 杨俐萍 《肿瘤学杂志》 CAS 2015年第6期507-511,共5页
恶性肿瘤的快速生长、转移与血管密切相关。大量研究表明,肿瘤血管生成细胞的来源存在多源性,血管生成的过程呈多样性,血管生成的调节呈多靶点性。这些特征提示研究者需拓宽思路,从不同层面理解肿瘤的新生血管生成机制,进而为寻找高度... 恶性肿瘤的快速生长、转移与血管密切相关。大量研究表明,肿瘤血管生成细胞的来源存在多源性,血管生成的过程呈多样性,血管生成的调节呈多靶点性。这些特征提示研究者需拓宽思路,从不同层面理解肿瘤的新生血管生成机制,进而为寻找高度选择性血管靶点的临床抗肿瘤药物提供新的思路。 展开更多
关键词 肿瘤血管生成 血管生成拟态 肿瘤微血管构筑表型异质性
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细胞衰老与肿瘤进展
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作者 丁丽丽 刘爱国 《国际儿科学杂志》 2018年第4期271-273,277,共4页
细胞衰老是细胞脱离细胞周期、丧失增殖能力后进入的一种相对稳定的状态,是抑制恶性肿瘤形成的重要机制。然而,越来越多的证据表明衰老细胞对周围的肿瘤微环境有负面影响,其中最重要的衰老相关性分泌表型(senescence—associated se... 细胞衰老是细胞脱离细胞周期、丧失增殖能力后进入的一种相对稳定的状态,是抑制恶性肿瘤形成的重要机制。然而,越来越多的证据表明衰老细胞对周围的肿瘤微环境有负面影响,其中最重要的衰老相关性分泌表型(senescence—associated secretory phenotype,SASP)可以促进肿瘤进展及复发。虽然现在对SASP及其与肿瘤发生的关系所知有限,但其为肿瘤的治疗研究提供了更多新的思路。 展开更多
关键词 细胞衰老 肿瘤进展 肿瘤相关性分泌表型
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miR-765通过Wnt/β-catenin信号通路调控甲状腺乳头状癌细胞生物学行为的研究 被引量:1
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作者 李锐 刘宏宇 +1 位作者 张洋 盖保东 《中华内分泌外科杂志》 CAS 2023年第4期430-434,共5页
目的探究miR-765在甲状腺乳头状癌(papillarythyroidcarcinoma,PTC)细胞中的作用及相关信号通路机制。方法利用qPCR检测miR-765在正常人甲状腺细胞系Nthy-ori3-1及人PTC细胞系B-CPAP和TPC-1中的表达。将PTC细胞分成空白对照组(blank con... 目的探究miR-765在甲状腺乳头状癌(papillarythyroidcarcinoma,PTC)细胞中的作用及相关信号通路机制。方法利用qPCR检测miR-765在正常人甲状腺细胞系Nthy-ori3-1及人PTC细胞系B-CPAP和TPC-1中的表达。将PTC细胞分成空白对照组(blank control,BC)、阴性对照组(negative control,NC)和miR-mimic组,BC组不做特殊处理,NC组转染阴性对照序列,miRNA模拟物(miR-mimic)组转染miR-765模拟物上调其表达。分别利用CCK-8实验、平板克隆形成实验、划痕实验和Transwell侵实验检测转染miR-mimic后PTC细胞的增殖、迁移及侵袭能力。利用Westernblot实验检测转染miR-mimic后PTC细胞中Wnt/β-catenin信号通路关键蛋白β-catenin的核内表达。结果与Nthy-ori 3-1细胞相比,PTC细胞中的miR-765表达显著下降(B-CPAP,P=0.0003;TPC-1,P=0.0003)。转染miR-mimic可显著上调PTC细胞中miR-765的表达(B-CPAP,P<0.0001;TPC-1,P<0.0001)。CCK-8实验(B-CPAP,P<0.05;TPC-1,P<0.05)、平板克隆形成实验(B-CPAP,P=0.0001;TPC-1,P<0.0001)、划痕实验(B-CPAP,P=0.0006;TPC-1,P<0.0001)及Tran-Swell侵袭实验(B-CPAP,P=0.001;TPC-1,P=0.0014)结果显示,上调miR-765的表达可显著抑制PTC细胞的增殖、迁移和侵袭能力。Western实验结果显示上调miR-765的表达可显著抑制PTC细胞中Wnt/β-catenin信号通路的水平(B-CPAP,P=0.0039;TPC-1,P=0.0004)。结论上调miR-765可抑制Wnt/β-catenin信号通路并抑制PTC细胞的增殖、迁移和侵袭能力,这不仅说明miR-765是PTC治疗的潜在新靶点,还进一步揭示了其发挥调控作用的机制。 展开更多
关键词 miR-765 Wnt/β-catenin信号通路 甲状腺乳头状癌 肿瘤细胞表型
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五种异质性弥漫大B细胞淋巴瘤微环境细胞PD-1表达及意义 被引量:2
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作者 周杰 杨文秀 王平 《中华肿瘤防治杂志》 CAS 北大核心 2020年第7期526-532,共7页
目的弥漫大B细胞淋巴瘤(diffuse large B cell lymphoma,DLBCL)是一组具有明显异质性的大细胞淋巴瘤,通过多种检查手段早期识别DLBCL预后较差的亚群,对于预后判断和治疗选择具有重要作用。本研究选择了几种相对少见免疫表型表达的DLBCL... 目的弥漫大B细胞淋巴瘤(diffuse large B cell lymphoma,DLBCL)是一组具有明显异质性的大细胞淋巴瘤,通过多种检查手段早期识别DLBCL预后较差的亚群,对于预后判断和治疗选择具有重要作用。本研究选择了几种相对少见免疫表型表达的DLBCL类型(C-myc/Bcl-2共表达、NF-κB/p65阳性、CD5阳性及CD30阳性的DLBCL)和EB病毒(epstein-barr virus,EBV)阳性的DLBCL病例进行研究,通过检测肿瘤微环境细胞中程序性死亡受体-1(programmed death receptor-1,PD-1)表达情况,探讨PD-1表达相关的影响因素及其表达对相应DLBCL发生和发展的影响。方法收集贵州医科大学附属医院2010-01-01-2018-08-31诊断的120例DLBCL患者资料,通过普通免疫组化染色(C-myc、Bcl-2、NF-κB/p65和CD30)、免疫组化双标(CD5、PAX5)及EBER原位杂交检测筛选出上述特殊类型的病例并分组,普通免疫组化染色检测肿瘤微环境中PD-1蛋白表达情况。收集临床病理资料并随访,对实验数据进行统计学分析。结果120例DLBCL患者中,PD-1肿瘤浸润淋巴细胞(tumor infiltraling lymphoeytes,TILs)阳性病例共有63例(52.5%,63/120);其中C-myc/Bcl-2共表达组中PD-1(+)-TILs病例27例(69.25%,27/39),NF-κB/p65阳性组30例(68.2%,30/44),CD30阳性组17例(73.9%,17/23),CD5阳性组14例(77.8%,14/18),EBV阳性组中为5例(55.6%,5/9)。PD-1(+)-TILs与PD-1(-)-TILs组病例之间IPI评分(χ^2=4.514,P=0.034)和Ann Arbor分期(χ^2=4.760,P=0.029)差异有统计学意义,PD-1(+)-TILs组分期晚、IPI指数高;PD-1-TILs表达在阳性组C-myc/Bcl-2共表达(χ^2=6.485,P=0.011)、NF-κB/p65(χ^2=6.851,P=0.009)、CD30(χ^2=5.232,P=0.022)、CD5(χ^2=5.426,P=0.040)表达率明显高于阴性组,差异有统计学意义;PD-1-TILs表达在EBV阳性组与阴性组之间差异无统计学意义,χ^2=0.001,P=0.970。生存分析98例随访病例中,PD-1(+)-TILs组病例的总生存率(overall survival,OS)明显低于PD-1(-)-TILs组患者,χ^2=14.652,P<0.001;在C-myc/Bcl-2共表达(χ^2=4.071,P=0.049)、NF-κB/p65的核表达(χ^2=4.833,P=0.025)及CD30阳性DLBCL(χ^2=5.207,P=0.024)中PD-1(+)-TILs组的生存状况也明显较PD-1(-)-TILs组病例差;多因素回归分析显示,PD-1(+)-TILs是DLBCL的独立危险因素,HR=38.170,P=0.036。结论PD-1表达在C-myc/Bcl-2共表达阳性、NF-κB/p65阳性、CD30阳性和CD5阳性的DLBCL中明显增高,在EBV阳性的DLBCL中增加不明显,PD-1在肿瘤微环境细胞中的表达可能是(C-myc/Bcl-2共表达阳性、NF-κB/p65阳性和CD30阳性)DLBCL不良预后评估的相关因素。 展开更多
关键词 弥漫大B细胞淋巴瘤 PD-1 肿瘤细胞免疫表型 EB病毒 免疫组化染色 预后
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HINT2在甲状腺乳头状癌中的表达和功能研究
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作者 张泸娇 李慧 +2 位作者 高维 李豪 刘丹慧 《中国细胞生物学学报》 CAS CSCD 2021年第4期789-796,共8页
HINT2与多种肿瘤发生发展密切相关,在甲状腺癌中的功能还不清楚。该文使用组织芯片检测甲状腺乳头状癌组织中HINT2的表达,发现12例癌组织中HINT2蛋白表达水平显著高于正常组织(86%,P<0.05)。该文构建高表达和低表达HINT2的甲状腺乳... HINT2与多种肿瘤发生发展密切相关,在甲状腺癌中的功能还不清楚。该文使用组织芯片检测甲状腺乳头状癌组织中HINT2的表达,发现12例癌组织中HINT2蛋白表达水平显著高于正常组织(86%,P<0.05)。该文构建高表达和低表达HINT2的甲状腺乳头状癌细胞系K1稳定克隆后,使用CCK8和克隆形成实验检测HINT2对细胞增殖能力的影响,采用Transwell和划痕法检测细胞迁移能力,使用Matrigel Transwell法检测细胞侵袭能力。结果发现,高表达HINT2促进了K1细胞的增殖、迁移和侵袭能力,而干扰HINT2则抑制了细胞的这些恶性表型。最后,该文用Western blot方法检测AKT和ERK的活化情况,发现高表达HINT2促进AKT和ERK的磷酸化,而干扰HINT2则抑制AKT和ERK的磷酸化。这些结果说明,HINT2可能是甲状腺乳头状癌的原癌基因,通过调控PI3K/AKT和MAPK/ERK通路影响甲状腺肉头状癌的恶性表型。 展开更多
关键词 甲状腺乳头状癌 肿瘤恶性表型 K1细胞株 HINT2
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