Objective: To study the expression of CD44 correlation with the ability ofmetastasis of tumor cells in gastric carcinoma, to find the correlation of the concentration ofsoluble CD 44 Variant 6 (sCD44v6) and the histol...Objective: To study the expression of CD44 correlation with the ability ofmetastasis of tumor cells in gastric carcinoma, to find the correlation of the concentration ofsoluble CD 44 Variant 6 (sCD44v6) and the histologic expression of CD44 Variant 6 (CD44v6) in tumorswith the clinico- pathologic features, and to make serum concentration of the CD44v6 and theexpression of CD44v6 may be useful an indicator as an early diagnosis, invasion, metastasis, andprognosis. Methods: Serum samples were obtained from 70 patients with primary gastric carcinomabefore surgery and 20 patients after surgery. Serum levels of CD44v6 were determined with aquantitative- enzyme- link-immuno- adsorbent assay. The expression of CD44v6 in tumors was examinedby immuno-histo-chemical staining. Results: Both the serum concentration of CD44v6 and itsexpression in tumors were positively related to the depth of invasion of the tumor, lymph nodemetastasis, clinical stage, and diffuse type gastric carcinoma, but not to the tumor size. The serumlevel of CD44v6 in patients with gastric cancer was significantly higher than that in control. Theserum concentration of CD44v6 was markedly lowered after curative surgery (F<0. 001), and the serumlevel of CD44v6 was higher in patients with gastric cancer with CD44v6 positive tumor cells than inthose with CD44v6 negative tumor cells. The serum level of CD44v6 was a prognostic indicator inpatients with diffuse type gastric carcinoma, as was the histological expression of CD44v6.Conclusion: CD44v6 of gastric cancer and serum concentration of CD44v6 seems to be correlated to theprogression of diffuse type gastric carcinoma metas- tasis and clinical stage. An elevated level ofsCD44v6 may serve as an indicator of lymph node metastasis (especially early metastasis) and badprognosis in pa tients with gastric carcinoma.展开更多
The research was conducted to study the effect of crude enzyme from cow rumen fluid on nutrient quality of cassava substrates through hydrolysis method. Completely randomized design （CRD） in this research consisted ...The research was conducted to study the effect of crude enzyme from cow rumen fluid on nutrient quality of cassava substrates through hydrolysis method. Completely randomized design （CRD） in this research consisted of 4 materials from cassava with its 11 combinations （the total was 15 treatments） with 3 replications. The fifteen treatments were tuber （T）, leaves （L）, peel （P）, onggok （O）, tuber ＋ leaves （TL）, peel ＋ tuber （PT）, tuber ＋ onggok （TO）, leaves ＋ peel （LP）, peel ＋ onggok （PO）, onggok ＋ leaves （OL）, leaves ＋ tuber ＋ peel （LTP）, leaves ＋ tuber ＋ onggok （LTO）, peel ＋ leaves ＋ onggok （PLO）, peel ＋ tuber ＋ onggok （PTO）, peel ＋ leaves ＋ tuber ＋ onggok （PLTO）. Rumen fluid was centrifuged at 10,000 rpm for 15 minutes in 4 ~C. Supernatant was reacted with ammonium sulphate （60%） and incubated in the freezer at 4 ~C for 24 hours. The ground cassava was added by rumen fluid crude enzyme at the dosage of 1% （b/v）. The cassava substrates were kept for 24 hours in room temperature. The addition of rumen fluid enzyme on cassava did not significantly affect dry matter losses （0.96%-2.08%）, but it significantly decreased crude fiber （8.61%-17.83%）. And On the other hand, it increased total sugar （15.19%-29.52%）. The conclusion of this research was that the addition of rumen fluid enzyme on cassava substrates was able to decrease crude fiber （17.83%） and the best total soluble sugar was in tuber （29.52%）, but for dry matter, it was similar to control in the range of 0.96%-2.08%.展开更多
基金Supported by a grant from Science and Technology Committee of Jiangsu Province(457-99064)
文摘Objective: To study the expression of CD44 correlation with the ability ofmetastasis of tumor cells in gastric carcinoma, to find the correlation of the concentration ofsoluble CD 44 Variant 6 (sCD44v6) and the histologic expression of CD44 Variant 6 (CD44v6) in tumorswith the clinico- pathologic features, and to make serum concentration of the CD44v6 and theexpression of CD44v6 may be useful an indicator as an early diagnosis, invasion, metastasis, andprognosis. Methods: Serum samples were obtained from 70 patients with primary gastric carcinomabefore surgery and 20 patients after surgery. Serum levels of CD44v6 were determined with aquantitative- enzyme- link-immuno- adsorbent assay. The expression of CD44v6 in tumors was examinedby immuno-histo-chemical staining. Results: Both the serum concentration of CD44v6 and itsexpression in tumors were positively related to the depth of invasion of the tumor, lymph nodemetastasis, clinical stage, and diffuse type gastric carcinoma, but not to the tumor size. The serumlevel of CD44v6 in patients with gastric cancer was significantly higher than that in control. Theserum concentration of CD44v6 was markedly lowered after curative surgery (F<0. 001), and the serumlevel of CD44v6 was higher in patients with gastric cancer with CD44v6 positive tumor cells than inthose with CD44v6 negative tumor cells. The serum level of CD44v6 was a prognostic indicator inpatients with diffuse type gastric carcinoma, as was the histological expression of CD44v6.Conclusion: CD44v6 of gastric cancer and serum concentration of CD44v6 seems to be correlated to theprogression of diffuse type gastric carcinoma metas- tasis and clinical stage. An elevated level ofsCD44v6 may serve as an indicator of lymph node metastasis (especially early metastasis) and badprognosis in pa tients with gastric carcinoma.
文摘The research was conducted to study the effect of crude enzyme from cow rumen fluid on nutrient quality of cassava substrates through hydrolysis method. Completely randomized design （CRD） in this research consisted of 4 materials from cassava with its 11 combinations （the total was 15 treatments） with 3 replications. The fifteen treatments were tuber （T）, leaves （L）, peel （P）, onggok （O）, tuber ＋ leaves （TL）, peel ＋ tuber （PT）, tuber ＋ onggok （TO）, leaves ＋ peel （LP）, peel ＋ onggok （PO）, onggok ＋ leaves （OL）, leaves ＋ tuber ＋ peel （LTP）, leaves ＋ tuber ＋ onggok （LTO）, peel ＋ leaves ＋ onggok （PLO）, peel ＋ tuber ＋ onggok （PTO）, peel ＋ leaves ＋ tuber ＋ onggok （PLTO）. Rumen fluid was centrifuged at 10,000 rpm for 15 minutes in 4 ~C. Supernatant was reacted with ammonium sulphate （60%） and incubated in the freezer at 4 ~C for 24 hours. The ground cassava was added by rumen fluid crude enzyme at the dosage of 1% （b/v）. The cassava substrates were kept for 24 hours in room temperature. The addition of rumen fluid enzyme on cassava did not significantly affect dry matter losses （0.96%-2.08%）, but it significantly decreased crude fiber （8.61%-17.83%）. And On the other hand, it increased total sugar （15.19%-29.52%）. The conclusion of this research was that the addition of rumen fluid enzyme on cassava substrates was able to decrease crude fiber （17.83%） and the best total soluble sugar was in tuber （29.52%）, but for dry matter, it was similar to control in the range of 0.96%-2.08%.
