AIM： To identify the methylation of secreted frizzled-related protein 1 （SFRP1） in gastric cancer and to investigate the aberrant expression of SFRP1 and its correlation with the clinical pathological features of p...AIM： To identify the methylation of secreted frizzled-related protein 1 （SFRP1） in gastric cancer and to investigate the aberrant expression of SFRP1 and its correlation with the clinical pathological features of patients. METHODS： We determined SFRP1 methylation and SFRP1 mRNA expression in 3 gastric cancer cell lines SGC-7901, BGC-823, HGC-27, from 52 primary gastric cancer specimens and matched tumor adjacent tissue specimens by methylation-specific （MSP） PCR and RT-PCR respectively. Fisher＇s exact test was used to analyze the statistical association between clinical pathological data and aberrant expression of SFRP1. RESULTS： In 3 cancer cell lines, BGC-823 and HGC-27 had methylated SFRP1 and lost SFRP1 mRNA expression. After treatment of BGC-823 and HGC-27 with the demethylating agent, 5-aza-2′-deoxycytidine, SFRP1 was re-expressed. In 52 primary gastric cancer specimens and matched tumor adjacent tissue specimens, hypermethylation of SFRP1 was detected in 23 （44%） and 8 （15%） specimens respectively （x^2= 10.34, P 〈 0.01）. Loss of SFRP1 expression was detected in 17（33%） and 6 （12%） specimens respectively （x^2= 6.75, P 〈 0.01）. There was a significant correlation between SFRP1 hypermethylation and SFRP1 expression loss. SFRP1 expression was also correlated significantly with tumor stage and lymph node status, but not with patient sex, age and histological type. CONCLUSION： SFRP1 inactivation is a common and early event caused mainly by hypermethylation in gastric cancer. SFRP1 expression loss may be correlated with tumor metastasis in primary gastric cancer.展开更多
AIM:To examine the association between parity and gastric cancer (the cases are almost all premenopausal women) risk in a cohort of young parous women.METHODS:The study cohort consisted of all women with a record of a...AIM:To examine the association between parity and gastric cancer (the cases are almost all premenopausal women) risk in a cohort of young parous women.METHODS:The study cohort consisted of all women with a record of a first and singleton childbirth in the Birth Register between 1978 and 1987.We tracked each woman from the time of her first childbirth to December 31,2008.Their vital status was ascertained by linking records to the computerized mortality database.Cox proportional hazard regression models were used to estimate hazard ratios of death from gastric cancer associated with parity.RESULTS:There were 1090 gastric cancer deaths (85.87% of them were premenopausal) during 33686828 person-years of follow-up.The mortality rate of gastric cancer was 3.24 cases per 100000 person-years.A trend of increasing risk of gastric cancer was seen with increasing parity.The adjusted hazard ratio was 1.24 [confidence interval (95% CI):1.02-1.50] for women who had borne two to three children,and 1.32 (95% CI:1.01-1.72) for women with four or more births,when compared with women who had given birth to only one child.CONCLUSION:These results suggest that higher parity may increase the risk of death from gastric cancer among premenopausal women.展开更多
基金Supported by Liaoning Education Divison Foundation, No.05L557
文摘AIM： To identify the methylation of secreted frizzled-related protein 1 （SFRP1） in gastric cancer and to investigate the aberrant expression of SFRP1 and its correlation with the clinical pathological features of patients. METHODS： We determined SFRP1 methylation and SFRP1 mRNA expression in 3 gastric cancer cell lines SGC-7901, BGC-823, HGC-27, from 52 primary gastric cancer specimens and matched tumor adjacent tissue specimens by methylation-specific （MSP） PCR and RT-PCR respectively. Fisher＇s exact test was used to analyze the statistical association between clinical pathological data and aberrant expression of SFRP1. RESULTS： In 3 cancer cell lines, BGC-823 and HGC-27 had methylated SFRP1 and lost SFRP1 mRNA expression. After treatment of BGC-823 and HGC-27 with the demethylating agent, 5-aza-2′-deoxycytidine, SFRP1 was re-expressed. In 52 primary gastric cancer specimens and matched tumor adjacent tissue specimens, hypermethylation of SFRP1 was detected in 23 （44%） and 8 （15%） specimens respectively （x^2= 10.34, P 〈 0.01）. Loss of SFRP1 expression was detected in 17（33%） and 6 （12%） specimens respectively （x^2= 6.75, P 〈 0.01）. There was a significant correlation between SFRP1 hypermethylation and SFRP1 expression loss. SFRP1 expression was also correlated significantly with tumor stage and lymph node status, but not with patient sex, age and histological type. CONCLUSION： SFRP1 inactivation is a common and early event caused mainly by hypermethylation in gastric cancer. SFRP1 expression loss may be correlated with tumor metastasis in primary gastric cancer.
基金Supported by A grant from the National Science Council,Executive Yuan,Taiwan,No.NSC-94-2314-B-037-056
文摘AIM:To examine the association between parity and gastric cancer (the cases are almost all premenopausal women) risk in a cohort of young parous women.METHODS:The study cohort consisted of all women with a record of a first and singleton childbirth in the Birth Register between 1978 and 1987.We tracked each woman from the time of her first childbirth to December 31,2008.Their vital status was ascertained by linking records to the computerized mortality database.Cox proportional hazard regression models were used to estimate hazard ratios of death from gastric cancer associated with parity.RESULTS:There were 1090 gastric cancer deaths (85.87% of them were premenopausal) during 33686828 person-years of follow-up.The mortality rate of gastric cancer was 3.24 cases per 100000 person-years.A trend of increasing risk of gastric cancer was seen with increasing parity.The adjusted hazard ratio was 1.24 [confidence interval (95% CI):1.02-1.50] for women who had borne two to three children,and 1.32 (95% CI:1.01-1.72) for women with four or more births,when compared with women who had given birth to only one child.CONCLUSION:These results suggest that higher parity may increase the risk of death from gastric cancer among premenopausal women.
