目的研究开发一种分子工具,通过内镜活检技术预测胃癌风险。方法收集2014至2018年共282例高风险胃癌患者,在其内镜样本中发现并验证了1个DNA甲基化生物标物组合,并在癌症基因组图谱项目(“TCGA”)的298个样本中进行了验证。采用全基因...目的研究开发一种分子工具,通过内镜活检技术预测胃癌风险。方法收集2014至2018年共282例高风险胃癌患者,在其内镜样本中发现并验证了1个DNA甲基化生物标物组合,并在癌症基因组图谱项目(“TCGA”)的298个样本中进行了验证。采用全基因组、表观基因组和基因特异性DNA甲基化分析,确定一个连续的生物标志物组合,该组合结合了总体DNA甲基化指数(global DNA methylation index,GDMI)和IRF4、ELMO1、CLIP4和MSC启动子DNA甲基化水平。结果比较未发生化生的胃炎患者(平均值=5.69,95%CI,4.88~6.45)、发生化生的胃炎患者(平均值=4.68,95%CI,3.67~5.73)和胃癌患者(平均值=3.36,95%CI,2.81~3.93)(P<0.0001),发现胃癌的GDMI与组织学进展呈负相关。IRF4(P<0.01)、ELMO1(P<0.01)、CLIP4(P<0.01)和MSC(P<0.01)启动子甲基化与从无化生的胃炎到有化生的胃炎再到胃癌的严重程度增加相关。结论IRF4、ELMO1、CLIP4和MSC启动子甲基化结合GDMI>4是内镜活检中胃癌风险分层的有效分子工具。展开更多
胃癌是常见的消化系统恶性肿瘤,是全球尤其东亚重要的公共卫生问题。通过筛查实现早诊早治是降低死亡率、提高生存率的重要手段。本文通过检索PubMed、Web of Science、知网、万方、维普等数据库,系统总结和分析了胃癌血清学筛查指标及...胃癌是常见的消化系统恶性肿瘤,是全球尤其东亚重要的公共卫生问题。通过筛查实现早诊早治是降低死亡率、提高生存率的重要手段。本文通过检索PubMed、Web of Science、知网、万方、维普等数据库,系统总结和分析了胃癌血清学筛查指标及各类指标组合方案方面的研究进展,并对胃癌筛查研究方向和应用提出展望。展开更多
Screening and early diagnosis of gastric cancer play important roles in reducing the mortality of gastric cancer. A vast amount of study data on gastric cancer screening and early diagnosis has been accumulated in and...Screening and early diagnosis of gastric cancer play important roles in reducing the mortality of gastric cancer. A vast amount of study data on gastric cancer screening and early diagnosis has been accumulated in and out of China in the past decades. The practice of gastric cancer screening has also been efficiently carried out in different countries and regions. However, no widely accepted principle of population screening for gastric cancer has been developed yet. Screening for gastric cancer requires extensive exploration both theoretically and practically. This article focuses on the method and program of gastric cancer screening based on population. Moreover, the current situation of gastric cancer screening and its evaluation are evaluated.展开更多
文摘目的研究开发一种分子工具,通过内镜活检技术预测胃癌风险。方法收集2014至2018年共282例高风险胃癌患者,在其内镜样本中发现并验证了1个DNA甲基化生物标物组合,并在癌症基因组图谱项目(“TCGA”)的298个样本中进行了验证。采用全基因组、表观基因组和基因特异性DNA甲基化分析,确定一个连续的生物标志物组合,该组合结合了总体DNA甲基化指数(global DNA methylation index,GDMI)和IRF4、ELMO1、CLIP4和MSC启动子DNA甲基化水平。结果比较未发生化生的胃炎患者(平均值=5.69,95%CI,4.88~6.45)、发生化生的胃炎患者(平均值=4.68,95%CI,3.67~5.73)和胃癌患者(平均值=3.36,95%CI,2.81~3.93)(P<0.0001),发现胃癌的GDMI与组织学进展呈负相关。IRF4(P<0.01)、ELMO1(P<0.01)、CLIP4(P<0.01)和MSC(P<0.01)启动子甲基化与从无化生的胃炎到有化生的胃炎再到胃癌的严重程度增加相关。结论IRF4、ELMO1、CLIP4和MSC启动子甲基化结合GDMI>4是内镜活检中胃癌风险分层的有效分子工具。
文摘胃癌是常见的消化系统恶性肿瘤,是全球尤其东亚重要的公共卫生问题。通过筛查实现早诊早治是降低死亡率、提高生存率的重要手段。本文通过检索PubMed、Web of Science、知网、万方、维普等数据库,系统总结和分析了胃癌血清学筛查指标及各类指标组合方案方面的研究进展,并对胃癌筛查研究方向和应用提出展望。
文摘Screening and early diagnosis of gastric cancer play important roles in reducing the mortality of gastric cancer. A vast amount of study data on gastric cancer screening and early diagnosis has been accumulated in and out of China in the past decades. The practice of gastric cancer screening has also been efficiently carried out in different countries and regions. However, no widely accepted principle of population screening for gastric cancer has been developed yet. Screening for gastric cancer requires extensive exploration both theoretically and practically. This article focuses on the method and program of gastric cancer screening based on population. Moreover, the current situation of gastric cancer screening and its evaluation are evaluated.
