三种脾气虚证模型大鼠血清胃泌素及胃窦G细胞的比较研究

目的:对3种脾气虚证模型大鼠的血清胃泌素及胃窦部胃泌素分泌细胞(G细胞)进行比较研究。方法:动物随机分为4组,即对照组、大黄组、双因素组(大黄法、游泳力竭法复合)、三因素组(大黄法、游泳力竭法、饥饿法复合)。检测血清胃泌素,胃窦G细胞等指标。结果:双因素组、三因素组的上述指标与对照组比较,差异有统计学意义(P<0.05,P<0.01),以三因素组为著(P<0.01)。结论:脾气虚证大鼠胃窦G细胞数量减少,释放胃泌素减少,血清胃泌素水平降低;三因素复合法制作脾气虚证动物模型效果似最优。

针刺胃病患者胃窦部胃泌素细胞变化研究

观察42例针刺中脘、内关、足三里治疗胃病患者的胃窦部胃泌素细胞（G细胞）变化。结果表明：针刺治疗前十二指肠溃疡患者冒粘膜G细胞数目增多，细胞内胃泌素含量增加，针刺治疗后G细胞数目和胃泌素含量均明显减少。慢性萎缩性胃炎患者，针刺治疗前G细胞数目少，变异不规则细胞多，且胃泌素物质扩散现象严重，针刺治疗后G细胞数目增多，胃泌素物质扩散现象消失。表明针刺可调节胃粘膜G细胞从不正常转向正常状态。

140例消化道疾病中血清胃泌素检测结果分析

胃泌素（gastrin，Gas）是主要由胃窦G细胞产生的胃肠肽类激素。其生理功能为促进胃酸分泌及胃黏膜营养作用和参与胃黏膜炎症反应等。近年来，由于放射免疫分析方法的建立，在消化道病变如炎症、溃疡及肿瘤的发生、发展过程中Gas发挥重要作用。我们检测了140例消化道疾病患者血清中的Gas的水平，现将结果报告如下。

Changes of G cells and D cells in the antral mucosa in rat experimental gastric ulcer

Objective: To investigate the association of changes in G and D cells in the antral mucosa with the production of gastrin and somatostatin during gastric ulcer and the healing process. Methods: Experimental gastric ulcer was induced with acetic acid in 42 Wistar rats and another 7 normal rats served as control. Changes in the production of gastrin and somatostatin in the plasma, gastric fluid and the antral tissues of the rats were measured by radio immunoassay, and the number and distribution of G and D cells were respectively determined by immunochemistry and Quantimet500 image analysis system. Results: In rats with gastric ulcer, the gastrin levels in the plasma, gastric fluid and the antral tissues increased while somatostatin levels were reduced, which were corrected in the healing process. Immunochemistry demonstrated the increase in the number of G cells in the antral tissues with decrease in D cell number, and the area covered by both cells shrank. The G cell to D cell number and area ratios were both decreased after the onset of the ulcer and returned to the normal when the healing process took place. Conclusion; Secretion of gastrin by G cells increases and that of somatostatin by D cells declines during gastric ulcer in rats, and imbalance of G and D cells may be responsible for gastrointestinal dysfunction.