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树突状细胞对胃粘膜免疫的调节作用研究 被引量:12
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作者 罗治彬 鲁荣 +3 位作者 罗元辉 晋华源 张朋彬 徐采朴 《第三军医大学学报》 CAS CSCD 北大核心 2000年第8期769-772,共4页
目的 通过研究胃粘膜中S 10 0 +,HLA DR+,CD4 +和CD8+产物的表达情况 ,以了解树突状细胞 (DC)在胃粘膜免疫中的作用。方法 胃癌 ( 176例 )、慢性萎缩性胃炎 ( 4 1例 )、不典型增生 ( 15例 )、肠上皮化生 ( 2 7例 )和正常对照组 ( 10... 目的 通过研究胃粘膜中S 10 0 +,HLA DR+,CD4 +和CD8+产物的表达情况 ,以了解树突状细胞 (DC)在胃粘膜免疫中的作用。方法 胃癌 ( 176例 )、慢性萎缩性胃炎 ( 4 1例 )、不典型增生 ( 15例 )、肠上皮化生 ( 2 7例 )和正常对照组 ( 10例 )共 2 69例 ,采用HE染色、S P免疫酶标抗体组化染色法与图像分析技术相结合 ,观察胃粘膜中S 10 0 +,HLA DR+,CD4 +和CD8+细胞数量、平均面积和平均吸光度的变化。结果 胃癌患者胃粘膜中S 10 0 +,HLA DR+细胞数量明显多于正常胃粘膜 (P <0 .0 5) ;CD4 +细胞及其平均吸光度显著减少 (P <0 .0 1) ;CD8+细胞数量、面积及其平均吸光度略有减少 ,但与正常组比较差异不显著 (P >0 .0 5) ;CD4 + CD8+比值为 0 .65,显著低于正常胃粘膜 (P <0 .0 1)。慢性萎缩性胃炎、肠上皮化生和不典型增生胃粘膜中S 10 0 +、HLA DR+细胞数量显著增加 (P <0 .0 1) ;CD8+细胞数量均明显高于正常胃粘膜 (P <0 .0 5) ;CD4 + CD8+比值分别为 0 .81,0 .59,0 .56,均显著低于正常胃粘膜 (P <0 .0 5)。结论 癌前状态胃粘膜中DC数量显著增多 ,功能增强 ;而胃癌患者胃粘膜中DC数量随分化程度的降低而减少。胃粘膜中DC细胞功能的抑制是导致胃粘膜免疫功能低下和癌前状态发生癌变的重要原因之一。 展开更多
关键词 DC细胞 胃粘膜免疫 癌前状态
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胃癌和癌前病变中树突状细胞的免疫组化研究 被引量:22
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作者 罗治彬 罗元辉 +3 位作者 鲁荣 晋华源 张朋彬 徐采朴 《世界华人消化杂志》 CAS 2000年第4期400-402,共3页
目的 通过研究胃粘膜中S100^+,HLA-DR^+,CD_4^+和CD_8^+产物的表达情况,以了解DC细胞在胃粘膜免疫中的作用。 方法 胃癌(143例)、慢性萎缩性胃炎(41例)、不典型增生(15例)、肠上皮化生(27例)和正常对照组(10例)共236例,都经内镜和病理... 目的 通过研究胃粘膜中S100^+,HLA-DR^+,CD_4^+和CD_8^+产物的表达情况,以了解DC细胞在胃粘膜免疫中的作用。 方法 胃癌(143例)、慢性萎缩性胃炎(41例)、不典型增生(15例)、肠上皮化生(27例)和正常对照组(10例)共236例,都经内镜和病理诊断明确,采用HE染色、SP免疫酶标抗体组化染色法与图象分析技术相结合,观察胃粘膜中S-100^+,HLA-DR^+,CD_4^+和CD_8^+细胞数量、平均面积和平均吸光度的变化。 结果 胃癌患者胃粘膜中S-100^+(0.6±0.2)μm^2,HLA-DR^+(0.9±0.2)细胞数量明显多于正常胃粘膜(P<0.05);CD_4^+细胞(0.7±0.2)μm^2及其平均吸光度显著减少(P<0.01);CD_8^+细胞数量(1.1±0.3)μm^2、面积及其平均吸光度约有减少,但与正常组比较无显著差异(P>0.05);CD_4^+/CD_8^+比值为0.7,显著低于正常胃粘膜(P<0.01),慢性萎缩性胃炎、肠上皮化生和不典型增生胃粘膜中S-100^+分别为(1.8±0.3)μm^2,(2.2±0.6)μm^2和(2.2±0.5)μm^2,HLA-DR^+分别为(2.4±0.6)μm^2,(3.0±0.8)μm^2和(2.8±0.6)μm^2细胞数量显著增加(P<0.01);CD_8^+细胞数量分别为(1.8±0.2)μm^2,(1.9±0.2)μm^2,(1.8±0.2)μm^2均明显高于正常胃粘膜(P<0.05);CD_4^+/CD_8^+比值分别为0.8,0.6,0.6,均显著低于正常胃粘膜(P<0.05)。 结论 展开更多
关键词 DC细胞 胃粘膜免疫 肿瘤 癌前病变
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Molecular cross-talk between Helicobacter pylori and human gastric mucosa 被引量:12
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作者 Vittorio Ricci Marco Romano Patrice Boquet 《World Journal of Gastroenterology》 SCIE CAS CSCD 2011年第11期1383-1399,共17页
Helicobacter pylori (H.pylori) has co-evolved with humans to be transmitted from person to person and to colonize the stomach persistently.A well-choreographed equilibrium between the bacterial effectors and host resp... Helicobacter pylori (H.pylori) has co-evolved with humans to be transmitted from person to person and to colonize the stomach persistently.A well-choreographed equilibrium between the bacterial effectors and host responses permits microbial persistence and health of the host,but confers a risk for serious diseases including gastric cancer.During its long coexistence with humans,H.pylori has developed complex strategies to limit the degree and extent of gastric mucosal damage and in? ammation,as well as immune effector activity.The present editorial thus aims to introduce and comment on major advances in the rapidly developing area of H.pylori/human gastric mucosa interaction (and its pathological sequelae),which is the result of millennia of co-evolution of,and thus of reciprocal knowledge between,the pathogen and its human host. 展开更多
关键词 Helicobacter pylori Gastric mucosa Pathogen/host interaction Gastric diseases Bacterial virulence factors CAGA VACA
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The expressions and interrelation of p130Cas and PTEN in gastric cancer
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作者 Zhou Wang Jifeng Li +1 位作者 Xichao Sun Xu Wang 《The Chinese-German Journal of Clinical Oncology》 CAS 2009年第6期332-335,共4页
Objective: To research the contributions of p130Cas and PTEN signal molecules to the carcinogenesis of gastric carcinoma and the relationship between them. Methods: Detecting proteins of p130Cas, PTEN and PTEN mRNA ... Objective: To research the contributions of p130Cas and PTEN signal molecules to the carcinogenesis of gastric carcinoma and the relationship between them. Methods: Detecting proteins of p130Cas, PTEN and PTEN mRNA of 76 cases normal gastric mucosa and 112 cases gastric carcinoma by immunohistochemistry EnVision method and molecular hybridization in situ method respectively. Detecting PTEN genetic mutation of 30 cases normal gastric mucosa, 7 cases early gastric cancer and 30 cases progressive gastric cancer by PCR-SSCP. Results: The expression of p130Cas protein of gastric carcinoma increased significantly than that of normal gastric mucosa (P 〈 0.05). Opposite to above, the expression of PTEN protein of gastric carcinoma group was significantly lower than that of normal gastric rnucosa group (P 〈 0.05). The expression of PTEN mRNA of gastric carcinoma group decreased obviously than normal gastric mucosa group (P 〈 0.001). Only one case exon 5 and one case exon 8 of PTEN appeared gene mutation of progressive gastric carcinoma group, the difference has no significance compared with normal gastric mucosa group and early gastric cancer group. Conclusion: The signaling molecules p130Cas and PTEN play an important role in the carcinogenesis of gastric carcinoma, and p130Cas plays the part of promoter, oppositely, maybe PTEN can inhibit it. 展开更多
关键词 signal protein P130CAS PTEN gastric carcinoma
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Mucosal Schwann cell “Hamartoma”:A new entity? 被引量:2
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作者 Paola Pasquini Andrea Baiocchini +4 位作者 Laura Falasca Dante Annibali Guido Gimbo Francesco Pace Franca Del Nonno 《World Journal of Gastroenterology》 SCIE CAS CSCD 2009年第18期2287-2289,共3页
Schwannoma is a well-described,benign nerve sheath tumor of the soft tissue,but is rare in the gastrointestinal tract.Gastrointestinal schwannomas are often incidentally discovered as small polypoid intraluminal lesio... Schwannoma is a well-described,benign nerve sheath tumor of the soft tissue,but is rare in the gastrointestinal tract.Gastrointestinal schwannomas are often incidentally discovered as small polypoid intraluminal lesions.In this report,we describe the clinicopathologic and immunohistochemical features of a distinctive neural mucosal polyp composed of a diffuse cellular proliferation of uniform bland spindled cells in the lamina propria that entraps the colonic crypts.Immunohistochemical analysis revealed strong and diffuse positivity for the S-100 protein.To avoid confusion of these solitary colorectal polyps containing pure spindled Schwann cell proliferation in the lamina propria with neural lesions that have significant association with inherited syndromes,it is better to use the designation "mucosal Schwann hamartoma". 展开更多
关键词 Nerve sheath tumors Gastrointestinal Schwannoma HAMARTOMA
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Expression of differential nitric oxide synthase isoforms in human gastric mucosa infected with Helicobacter pylori
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作者 屠振兴 龚燕芳 +3 位作者 丁华 许国铭 李兆申 满晓华 《Journal of Medical Colleges of PLA(China)》 CAS 2003年第5期286-289,共4页
Objective: To study the relationship between nitric oxide synthase (NOS) expression in human gastric mucosa and Helicobacter pylori (H.pylori) infection. Methods: Gastric mucosa samples were obtained from antrum of 33... Objective: To study the relationship between nitric oxide synthase (NOS) expression in human gastric mucosa and Helicobacter pylori (H.pylori) infection. Methods: Gastric mucosa samples were obtained from antrum of 33 patients received gastroendoscopy. H.pylori infection was confirmed by Giems staining and bacteria culture under microaerophilic conditions. Expression of iNOS, eNOS and nitrotyrosine were detected by immunohistochemistry. Results: (1) The positive rate of H. pylori infection was 66.7%(22/33). (2) iNOS positive staining in inflammatory cells was detected in 77.3%(17/22) of samples with H.pylori and 27.3%(3/11) without H.pylori infection (P<0.01). (3) eNOS expression in inflammatory cells was found in 77.3%(17/22) of samples with H. pylori and 18.2%(2/11) without H.pylori infection (P<0.01). (4) Nitrotyrosine expression in inflammatory cells was observed in 59.1%(13/22) of samples with H. pylori and 54.5%(6/11) without H. pylori infection (P>0.05). (5) Moderate and severe infiltrations of inflammatory cells were found in 86.4%(19/22) of gastric biopsies with H. pylori and 9.1%(1/11) of samples without H. pylori infection (P<0.01). Conclusion: H.pylori infection might promote infiltration of mononuclear cells and macrophages in gastric mucosa and induce iNOS expression in these cells. The accumulated nitric oxide in local area may result in gastric mucosa damage. 展开更多
关键词 H. pylori nitric oxide synthase IMMUNOHISTOCHEMISTRY
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