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低压低氧致小鼠胃肠应激损伤的研究
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作者 魏雪 尚慧莹 +3 位作者 叶岸平 肖成荣 高月 马增春 《中国药理学通报》 CAS CSCD 北大核心 2024年第8期1482-1488,共7页
目的通过低压氧舱模拟高原缺氧环境,探究缺氧不同时间对小鼠胃肠应激损伤的影响。方法根据低压低氧不同时间设置正常对照组、低压低氧1、3、5天模型组,低压低氧处理完成后检测小肠推进率、内脏敏感性变化及胃肠道病理损伤程度;酶联免疫... 目的通过低压氧舱模拟高原缺氧环境,探究缺氧不同时间对小鼠胃肠应激损伤的影响。方法根据低压低氧不同时间设置正常对照组、低压低氧1、3、5天模型组,低压低氧处理完成后检测小肠推进率、内脏敏感性变化及胃肠道病理损伤程度;酶联免疫吸附法(ELISA)检测胃肠道IL-1β、IL-6、TNF-α含量变化,肠组织胃肠激素及肠黏膜损伤标志物二胺氧化酶、D-乳酸含量变化;Western blot实验检测肠组织紧密连接蛋白及紧密连接损伤通路相关蛋白表达变化。结果与对照组相比,模型组小鼠小肠推进率加快,肠道敏感性升高;肠黏膜损伤标志物表达升高;肠组织抑制胃肠蠕动激素降低,促胃肠蠕动激素升高;紧密连接蛋白ZO-1、claudin-1、occludin及紧密连接损伤相关通路蛋白VASP表达降低,紧密连接损伤相关通路中的NF-κB、HIF-1α、VEGF表达升高;胃肠组织IL-1β、IL-6、TNF-α表达升高;胃组织胃蛋白酶含量升高;胃肠组织1天组损伤较轻,3天、5天组损伤较重;结论在低氧低氧环境中不同时间小鼠胃肠发生应激损伤,暴露3天时胃肠功能损伤较为严重,其中肠损伤机制可能与NF-κB/HIF-1α/VEGF/VASP信号通路被激活有关。 展开更多
关键词 低压低氧 小鼠 胃肠 小肠推进率 肠黏膜损伤 紧密连接
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高原胃肠应激反应的病理变化与肠道菌群调控作用的研究进展
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作者 赵海涛 马力天 +3 位作者 林强 李嘉辉 口志福 王景杰 《山西医科大学学报》 CAS 2024年第7期936-941,共6页
人在短时间内从平原进入高原地区,海拔高度快速升高,机体会产生一系列缺氧表现。这些反应包括但不限于头痛、失眠、食欲减退等症状,严重时甚至可能对人体器官和系统造成损害,威胁生命[1]。近年来,急性高原病(acute altitude sickness,A... 人在短时间内从平原进入高原地区,海拔高度快速升高,机体会产生一系列缺氧表现。这些反应包括但不限于头痛、失眠、食欲减退等症状,严重时甚至可能对人体器官和系统造成损害,威胁生命[1]。近年来,急性高原病(acute altitude sickness,AMS)导致的胃肠应激症状在不同人群中均有发生,逐渐受到关注。一项针对从平原地区急进高原驻训官兵的研究表明,一定时间内进驻不同海拔高度(3300,4000,4300,4700 m)高原3 d内消化系统症状发生率依次为纳差(54.7%)、恶心(31.1%)、腹胀(25.1%)、腹泻(23.3%)、便秘(18.3%)[2]。在1025名西藏大学新生中,出现高原反应症状的有642名,反应率为62.63%,其中有消化道反应者有378人,占高反学生的58.88%,症状以纳差、恶心、呕吐、口腔黏膜出血、腹胀、腹泻、腹痛、呕血以及黑便为主[3]。急进高原环境诱发高原胃肠道应激反应发病率居高不下,且症状多样,起病隐匿,极大地影响了短期进驻高原地区人群的身体健康。 展开更多
关键词 肠道菌群 肠黏膜屏障 高原 缺氧 胃肠反应
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消痰通腑颗粒治疗急性重症应激性胃肠反应的临床研究 被引量:3
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作者 叶敏 孙大志 +6 位作者 修丽娟 刘煊 秦志丰 施俊 武峰 陆烨 魏品康 《中国中医急症》 2013年第4期524-525,共2页
目的观察消痰通腑颗粒对急性重症应激性胃肠反应的治疗效果。方法纳入急性重症应激性胃肠反应患者随机分为两组,同时接受常规基础治疗,治疗组在此基础上加服消痰通腑颗粒,疗程为7 d。两组均在治疗前与治疗后应用胃肠疾病中医证候评分表... 目的观察消痰通腑颗粒对急性重症应激性胃肠反应的治疗效果。方法纳入急性重症应激性胃肠反应患者随机分为两组,同时接受常规基础治疗,治疗组在此基础上加服消痰通腑颗粒,疗程为7 d。两组均在治疗前与治疗后应用胃肠疾病中医证候评分表进行测评,进行胃动素及胃泌素检测,并观察干预后首次排便时间。结果两组胃肠疾病中医证候评分均呈下降趋势,治疗组下降幅度大于对照组。两组胃动素及胃泌素水平均明显升高;治疗组首次排便时间明显早于对照组。结论消痰通腑颗粒可有效改善急性重症应激性胃肠反应。 展开更多
关键词 消痰通腑颗粒 急性重症应胃肠反应 胃肠疾病中医证候评分表 胃动素 胃泌素
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复方谷氨酰胺肠溶胶囊治疗高原胃肠应激综合征的临床观察 被引量:2
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作者 陈奇 王艳丽 +5 位作者 严炜 仇忠辉 彭建 吴昊 李超 付麟力 《西南军医》 2012年第5期724-725,共2页
目的观察复方谷氨酰胺肠溶胶囊治疗高原胃肠应激综合征的临床疗效。方法将100例某汽车部队患高原胃肠应激综合征的患者随机分为治疗组及对照组,各50例。治疗组为常规治疗基础上加用复方谷氨酰胺肠溶胶囊,对照组仅予常规治疗,于治疗第3... 目的观察复方谷氨酰胺肠溶胶囊治疗高原胃肠应激综合征的临床疗效。方法将100例某汽车部队患高原胃肠应激综合征的患者随机分为治疗组及对照组,各50例。治疗组为常规治疗基础上加用复方谷氨酰胺肠溶胶囊,对照组仅予常规治疗,于治疗第3天及第7天观察疗效。结果治疗第3天治疗组总有效率为90.0%,对照组为74.0%,两组差异有统计学意义(P<0.05),第7天治疗组总有效率为98.0%,对照组为90.0%,两组差异无统计学意义(P>0.05)。结论针对川藏线汽车部队高原胃肠应激综合征在常规治疗基础上加用复方谷氨酰胺肠溶胶囊是安全有效的,且较常规治疗有更好的疗效,对缩短病程也有一定作用。 展开更多
关键词 高原 胃肠综合征 谷氨酰胺
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半夏泻心汤加减治疗老年反流性食管炎的效果及对食管动力和胃肠激素的影响
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作者 吴迪 《中文科技期刊数据库(全文版)医药卫生》 2023年第4期97-100,共4页
研究半夏泻心汤的中医及现代医学作用机理,并观察其治疗老年反流性食管炎(RE)的效果。方法:随机分组路径下将90例脾胃湿热证老年RE患者分为XY组45例和XY+BX组45例。XY组患者所应用的治疗药物均为常规性的西医药物,XY+BX组则在西医药物... 研究半夏泻心汤的中医及现代医学作用机理,并观察其治疗老年反流性食管炎(RE)的效果。方法:随机分组路径下将90例脾胃湿热证老年RE患者分为XY组45例和XY+BX组45例。XY组患者所应用的治疗药物均为常规性的西医药物,XY+BX组则在西医药物治疗的同时辅助应用半夏泻心汤,并对症予以方剂加减。对比两组患者的临床疗效,并于治疗前后分别检测食管动力学指标(LESP、CA和反流频次)和胃肠激素指标(血清MOT、GAS、VIP)。结果:经治疗后,在中医症候积分的改善效果上,XY+BX组患者获得的改善幅度优于XY组( P<0.05)。在治疗有效率上,XY+BX组对比XY组为93.33%,77.78%)( P<0.05)。治疗后,AM+SL组患者LESP、CA均高于XY组( P<0.05),反流频次低于XY组( P<0.05)。治疗后,在血清MOT、GAS、VIP各指标值的改善幅度上,AM+SL组均优于XY组( P<0.05)。结论:半夏泻心汤具有丰富的中医及现代医学药用价值,在老年RE治疗中有利于调节患者胃肠激素的平衡,并提高食管动力障碍,使患者获得更好的治疗效果。 展开更多
关键词 老年反流性食管炎 半夏泻心汤 中医症候 食管动力 胃肠激
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高海拔地区部队官兵胃肠应激调查与内镜学评价 被引量:7
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作者 王昕 马贤纵 +6 位作者 金鹏 潘元明 许俊锋 何玉琦 康倩 王晓英 盛剑秋 《解放军医学院学报》 CAS 2019年第10期919-922,942,共5页
目的了解高海拔地区部队官兵胃肠应激反应发生率、相关理化指标和胃镜下的黏膜评价,为高原胃肠应激防控提供依据。方法2018年1月选取格尔木(377人,平均海拔3000 m)、玉树(237人,平均海拔4200 m)地区共614名部队官兵,通过自制《部队官兵... 目的了解高海拔地区部队官兵胃肠应激反应发生率、相关理化指标和胃镜下的黏膜评价,为高原胃肠应激防控提供依据。方法2018年1月选取格尔木(377人,平均海拔3000 m)、玉树(237人,平均海拔4200 m)地区共614名部队官兵,通过自制《部队官兵胃肠流行病学问卷调查表》进行流行病学调查,现场检测血清幽门螺杆菌(Helicobacter pylori,Hp)抗体、便隐血,分析胃肠症状与Hp感染、便隐血的相关性及对有胃肠应激反应的官兵进行便携式一体化胃镜检查。结果格尔木、玉树两地官兵胃肠应激反应发生率高达50.8%(312/614),其中以腹泻38.4%(236/614)、食欲减退35%(215/614)、腹胀27.9%(171/614)、腹痛17.6%(108/614)、口臭17.6%(108/614)、呕血/便血7.3%(45/614)多见。格尔木地区官兵腹泻[44.6%(168/377)vs 28.7%(68/237)]、口臭[21.5%(81/377)vs 11.4%(27/237)]、呕血/便血[9.3%(35/377)vs 4.2%(10/237)]发生率均高于玉树地区,差异均有统计学意义(P均<0.05)。两地官兵现症Hp平均感染率为49.8%(360/614),其中格尔木地区为49.3%(186/377),玉树地区为50.6%(120/237),差异无统计学意义。便隐血检出率为24.9%(153/614),其中格尔木地区官兵为15.4%(58/377),显著低于玉树地区的40.1%(95/237)(P<0.001)。其中212名有胃肠应激症状官兵行胃镜检查发现,均罹患不同程度的胃食管、十二指肠炎性改变,以胃炎、溃疡、黏膜糜烂、出血多见。结论高海拔地区胃肠应激反应发生率高,可引起食欲缺乏、慢性腹胀、腹泻、腹痛等,应引起足够重视,积极探索有效的防治措施,提高高原卫勤保障能力。 展开更多
关键词 高原 胃肠 幽门螺杆菌感染 便隐血 军事人员
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脑出血合并急性应激性胃肠黏膜病变 被引量:1
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作者 张国富 《现代实用医学》 2003年第10期641-641,共1页
关键词 脑出血 合并症 急性应胃肠黏膜病变 治疗 预后
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高原胃肠应激反应1例
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作者 王希峰 《西南国防医药》 CAS 2011年第7期811-811,共1页
病例女,24岁,从平原乘飞机转火车人海拔4500m的高原地区,当晚出现头痛、气促、腹胀、失眠。根据病史和症状,诊断为急性高原反应。嘱其注意休息,予间断吸氧后好转。次日午餐半小时后,上腹胀痛,腹泻3次,水样便,大约1000ml,
关键词 高原 胃肠反应
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中药抗炎灵联合奥美拉唑钠治疗烧伤患者胃肠道功能衰竭的临床观察 被引量:2
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作者 宋知仁 刘志云 +1 位作者 成路 冷明芳 《中国中西医结合杂志》 CAS CSCD 北大核心 2008年第3期258-260,共3页
目的观察中药抗炎灵煎剂联合奥美拉唑钠治疗烧伤患者胃肠道功能衰竭的疗效。方法烧伤后胃肠道功能衰竭患者随机分为两组,治疗组32例(其中急性应激性胃肠黏膜病变伴出血18例,中毒性肠麻痹14例),对照组20例(分别为11例和9例)治疗组用中药... 目的观察中药抗炎灵煎剂联合奥美拉唑钠治疗烧伤患者胃肠道功能衰竭的疗效。方法烧伤后胃肠道功能衰竭患者随机分为两组,治疗组32例(其中急性应激性胃肠黏膜病变伴出血18例,中毒性肠麻痹14例),对照组20例(分别为11例和9例)治疗组用中药抗炎灵煎剂加奥美拉唑钠,对照组单用奥美拉唑钠,并观察治疗前和治疗12 h后胃黏膜内pH值,48 h内急性应激性胃肠黏膜病变伴出血的止血效果和治疗后72 h内中毒性肠麻痹的治疗效果。结果治疗前两组患者胃黏膜内pH明显低于正常值;治疗后治疗组胃黏膜内pH值上升(P<0.05)并接近正常,而对照组上升不明显。急性应激性胃肠黏膜病变伴出血治疗组显效8例,有效6例,无效4例,总有效率为77.8%,对照组分别为2例、3例、6例及45.5%:两组比较差异有统计学意义(P<0.05)。中毒性肠麻痹治疗组显效9例,有效3例,无效2例,总有效率为85.7%,对照组均无效,两组比较差异有统计学意义(P<0.05)。结论中药抗炎灵联合奥美拉唑钠对烧伤患者胃肠道功能衰竭有明显的治疗作用。 展开更多
关键词 中药抗炎灵 奥美拉唑钠 烧伤后胃肠道功能衰竭 急性应胃肠黏膜病变 中毒性肠麻痹
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根除幽门螺杆菌对功能性消化不良疗效的影响 被引量:1
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作者 王莉 黄留业 +1 位作者 李绥军 吴承荣 《中华腹部疾病杂志》 2004年第2期99-100,共2页
目的 通过根除幽门螺杆菌(HP)治疗,观察对功能性消化不良(FD)病人近期和远期疗效的影响,探讨HP与FD的关系。方法 选择100例HP阳性的FD病人,随机分成2组。对照组给予施维舒2粒,3次/d,和(或)吗丁啉10mg,3次/d,疗程1个月,治疗组... 目的 通过根除幽门螺杆菌(HP)治疗,观察对功能性消化不良(FD)病人近期和远期疗效的影响,探讨HP与FD的关系。方法 选择100例HP阳性的FD病人,随机分成2组。对照组给予施维舒2粒,3次/d,和(或)吗丁啉10mg,3次/d,疗程1个月,治疗组在此基础上于疗程第1周加用果胶铋2粒,3次/d(或洛赛克20mg,2次/d),克拉霉素0.5,2次/d,替硝唑0.5,2次/d。于治疗前和疗程结束后1周、6个月分别进行症状评分,两组动力障碍型亚组病人行钡条法胃固体排空试验。将上述结果进行治疗前后的比较。结果 疗程结束后1周和6个月症状评分与治疗前比较,治疗组显效率和总有效率较对照组均明显提高,差异有显著性(P<0.01),胃固体排空试验结果显示,疗程结束后1周,治疗组改善率较对照组差异无显著性(P>0.05),但疗程结束后6个月两组间差异有显著性(P<0.01)。结论 根除HP可显著提高FD病人近期和远期疗效。提示HP感染与FD之间存在明显关系。 展开更多
关键词 幽门螺杆菌 HP 功能性消化不良 FD 治疗 胃肠激
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Gastrointestinal hormone abnormalities and G and D cells in functional dyspepsia patients with gastric dysmotility 被引量:27
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作者 Mei-RongHe Yu-GangSong Fa-ChaoZhi 《World Journal of Gastroenterology》 SCIE CAS CSCD 2005年第3期443-446,共4页
AIM: To investigate the relationship between gastric dysmotility,gastrointestinal hormone abnormalities, and neuroendocrine cells in gastrointestinal mucosa in patients with functional dyspepsia (FD).METHODS: Gastric ... AIM: To investigate the relationship between gastric dysmotility,gastrointestinal hormone abnormalities, and neuroendocrine cells in gastrointestinal mucosa in patients with functional dyspepsia (FD).METHODS: Gastric emptying was assessed with solid radiopaque markers in 54 FD patients, and the patients were divided into two groups according to the results, one with delayed gastric emptying and the other with normal gastric emptying. Seventeen healthy volunteers acted as normal controls. Fasting and postprandial plasma levels and gastroduodenal mucosal levels of gastrointestinal hormones gastrin, somatostatin (SS) and neurotensin (NT)were measured by radioimmunoassay in all the subjects.G cells (gastrin-producing cells) and D cells (SS-producing cells) in gastric antral mucosa were immunostained with rabbit anti-gastrin polyclonal antibody and rabbit anti-SS polyclonal antibody, respectively, and analyzed quantitatively by computerized image analysis.RESULTS: The postprandial plasma gastrin levels, the fasting and postprandial plasma levels and the gastric and duodenal mucosal levels of NT were significantly higher in the FD patients with delayed gastric emptying than in those with normal gastric emptying and normal controls. The number and gray value of G and D cells and the G cell/D cell number ratio did not differ significantly between normal controls and the FD patients with or without delayed gastric emptying.CONCLUSION: Our findings suggest that the abnormalities of gastrin and NT may play a role in the pathophysiology of gastric dysmotility in FD patients, and the abnormality of postprandial plasma gastrin levels in FD patients with delayed gastric emptying is not related to the changes both in the number and gray value of G cells and in the G cell/D cell number ratio in gastric antral mucosa. 展开更多
关键词 Functional dyspepsia Gastric emptying Gastrointestinal hormone abnormalities G cells D cells
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Mechanisms underlying feed intolerance in the critically ill: Implications for treatment 被引量:18
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作者 Adam Deane Marianne J Chapman +3 位作者 Robert J Fraser Laura K Bryant Carly Burgstad Nam Q Nguyen 《World Journal of Gastroenterology》 SCIE CAS CSCD 2007年第29期3909-3917,共9页
Malnutrition is associated with poor outcomes in critically ill patients. Although nutritional support is yet to be proven to improve mortality in non-malnourished critically ill patients, early enteral feeding is con... Malnutrition is associated with poor outcomes in critically ill patients. Although nutritional support is yet to be proven to improve mortality in non-malnourished critically ill patients, early enteral feeding is considered best practice. However, enteral feeding is often limited by delayed gastric emptying. The best method to clinically identify delayed gastric emptying and feed intolerance is unclear. Gastric residual volume (GRV) measured at the bedside is widely used as a surrogate marker for gastric emptying, but the value of GRV measurement has recently been disputed. While the mechanisms underlying delayed gastric emptying require further investigation, recent research has given a better appreciation of the pathophysiology. A number of pharmacological strategies are available to improve the success of feeding. Recent data suggest a combination of intravenous metoclopramide and en/thromycin to be the most successful treatment, but novel drug therapies should be explored. Simpler methods to access the duodenum and more distal small bowel for feed delivery are also under investigation. This review summarises current understanding of the factors responsible for, and mechanisms underlying feed intolerance in critical illness, together with the evidence for current practices. Areas requiring further research are also highlighted. 展开更多
关键词 Critical illness Enteral nutrition Gastric emptying Gastric motility Gastrointestinal hormones METOCLOPRAMIDE ERYTHROMYCIN Prokinetic therapy
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EFFECTS OF ENTERAL AND PARENTERAL NUTRITION ON GASTROENTERIC HORMONES AND GASTRIC MOTILITY AFTER SUBTOTAL GASTRECTOMY 被引量:4
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作者 Wei-ming Kang Jian-chun Yu +2 位作者 Qun Zhang Mei-yun Ke Jia-ming Qian 《Chinese Medical Sciences Journal》 CAS CSCD 2008年第2期113-116,共4页
Objective To investigate the effects of enteral nutrition (EN) and parenteral nutrition (PN) on gastric motility and gastroentefic hormones after subtotal gastrectomy. Methods Forty-one patients underwent gastrec... Objective To investigate the effects of enteral nutrition (EN) and parenteral nutrition (PN) on gastric motility and gastroentefic hormones after subtotal gastrectomy. Methods Forty-one patients underwent gastrectomy were randomly divided into EN group (n=20) and PN group (n=21 ). From the first postoperative day to the seventh day, patients received either EN (EN group) or PN (PN group) with isocalofic (84.9kJ·kg^-1·d^-1) and isonitrogenous (0.11g·kg^-1·d^-1) intake. Serum gastrin (GAS), plasma mofilin (MTL), and plasma cholecystokinin (CCK) were measured on preoperative day, the first and seventh postoperative day. Electrogastrography (EGG) was measured on preoperative day and the seventh postoperative day. Results Compared with preoperafion, blood GAS, MTL, and CCK levels of 41 patients decreased significantly on the first day after subtotal gastrectomy ( P 〈 0. 001 ), but returned to the preoperative levels one week later. EGG after gastrectomy showed that gastric basal electrical rhythm was significantly restrained ( P 〈 0. 001 ). On the seventh day after subtotal gastrectomy, plasma MTL and CCK levels in EN group were higher than those in PN group ( P 〈 0.05 ). There was no difference in GAS level between two groups. EGG in EN group was better than that in PN group postoper- atively. Conclusions The levels of gastroentefitic hormones and the gastric motility decrease significantly after subtotal gastrectomy. In contrast with PN, EN can accelerate the recovery of MTL, CCK, and gastric motility after subtotal gastrectomy. 展开更多
关键词 enteral nutrition parenteral nutrition gastroenteric hormones gastric motility GASTRECTOMY
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Confocal laser endomicroscopy and immunoendoscopy for real-time assessment of vascularization in gastrointestinal malignancies 被引量:6
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作者 Dan Ionut Gheonea Tatiana Crtnǎ +3 位作者 Tudorel Ciurea Carmen Popescu Anca Bǎdǎrǎu Adrian Sǎftoiu 《World Journal of Gastroenterology》 SCIE CAS CSCD 2011年第1期21-27,共7页
Gastrointestinal cancers represent a major cause of morbidity and mortality,with incomplete response to chemotherapy in the advanced stages and poor prognosis.Angiogenesis plays a crucial part in tumor growth and meta... Gastrointestinal cancers represent a major cause of morbidity and mortality,with incomplete response to chemotherapy in the advanced stages and poor prognosis.Angiogenesis plays a crucial part in tumor growth and metastasis,with most gastrointestinal cancers depending strictly on the development of a new and devoted capillary network.Confocal laser endomicroscopy is a new technology which allows in vivo microscopic analysis of the gastrointestinal mucosa and its microvascularization during ongoing endoscopy by using topically or systemically administered contrast agents.Targeting markers of angiogenesis in association with confocal laser endomicroscopic examination(immunoendoscopy),as a future challenge,will add functional analysis to the morphological aspect of the neoplastic process.This review describes previous experience in endomicroscopic examination of the upper and lower digestive tract with emphasis on vascularization,resulting in a broad spectrum of potential clinical applications,and also preclinical research that could be translated to human studies. 展开更多
关键词 Confocal laser endomicroscopy Immunoendoscopy Fluoresceine ACRIFLAVINE CANCER
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Relationship between expression of gastrin, somatostatin,Fas/FasL and caspases in large intestinal carcinoma 被引量:15
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作者 Jia-Ding Mao Pei Wu Ying-Lin Yang Jian Wu He Huang 《World Journal of Gastroenterology》 SCIE CAS CSCD 2008年第18期2802-2809,共8页
AIM: To explore the correlation between the mRNAs and protein expression of gastrin (GAS), somatostatin (SS) and apoptosis index (AI), apoptosis regulation gene Fas/ FasL and caspases in large intestinal carcinoma (LI... AIM: To explore the correlation between the mRNAs and protein expression of gastrin (GAS), somatostatin (SS) and apoptosis index (AI), apoptosis regulation gene Fas/ FasL and caspases in large intestinal carcinoma (LIC). METHODS: Expression of GAS and SS mRNAs were detected by nested RT-PCR in 79 cases of LIC. Cell apoptosis was detected by molecular biology in situ apoptosis detecting methods (TUNEL). Immunohistochemical staining for GAS, SS, Fas/FasL, caspase-3 and caspase-8 was performed according to the standard streptavidin-biotin-peroxidase (S-P) method. RESULTS: There was a significant positive correlation between mRNA and protein expression of GAS and SS (GASrs=0.99, P < 0.01; SSrs = 0.98, P < 0.01). There was significant difference in positive expression rates of GAS, SS mRNAs and protein among different histological differentiation, histological types and Dukes’ stage of LIC. The AI in GAS high and moderate expression groups was significantly lower than that in low expression groups (3.75 ± 2.38 vs 7.82 ± 2.38, P < 0.01; 5.51 ± 2.66 vs 7.82 ± 2.38, P < 0.01), and the AI in SS high and moderate expression groups was significantly higher than that in low expression groups (9.03 ± 1.76 vs 5.35 ± 3.00, P < 0.01; 7.44 ± 2.67 vs 5.35 ± 3.00, P < 0.01). There was a significant negative correlation between the integral ratio of GAS to SS and the AI (rs = -0.41, P < 0.01). The positive expression rate of FasL in GAS high and moderate expression groups was higher than thatin low expression group (90.9% and 81.0% vs 53.2%, P < 0.05). The positive expression rates of Fas, caspase-8 and caspase-3 in SS high (90.0%, 90.0% and 100%) and moderate (80.0%, 70.0%, 75.0%) expression groups were higher than that in low expression group (53.1%, 42.9%, 49.0%) (90.0% and 80.0% vs 53.1%, P < 0.05; 90.0% and 70.0% vs 42.9%, P < 0.05; 100.0% and 75.0% vs 49.0%, P < 0.05). There was a significant positive correlation between the integral ratio of GAS to SS and the semiquantitative integral of FasL (rs = 0.32, P < 0.01). CONCLUSION: GAS and SS play important roles in the regulation and control of cell apoptosis in LIC, and the mechanism may be directly related to the aberrant expression of Fas/FasL. The GAS and SS will be valuable targets of the biological behavior of LIC. 展开更多
关键词 Large intestinal carcinoma GASTRIN SOMATOSTATIN Apoptosis index Fas FasL Caspase
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EFFECTS OF ELECTRO-ACUPUNCTURE ON SOMATOSTATIN AND PANCREATIC POLYPEPTIDE IN ISCHEMIC CEREBROVASCULAR DISEASES 被引量:2
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作者 张小澍 袁玉民 +3 位作者 匡培根 吴卫平 张凤英 刘洁晓 《Journal of Traditional Chinese Medicine》 SCIE CAS CSCD 1999年第1期54-58,共5页
The levels of somatostatin(SS)in CSF and blood and pancreatic polypeptide(PP)inplasma were measured by radioimmunoassay in 64 patients with acute ischemiccerebrovascular diseases(ICVD),randomly divided into two groups... The levels of somatostatin(SS)in CSF and blood and pancreatic polypeptide(PP)inplasma were measured by radioimmunoassay in 64 patients with acute ischemiccerebrovascular diseases(ICVD),randomly divided into two groups:group 1(n=31,bothelectro-acupuncture and routine treatments given)and group 2(n=33,routine treatment)and 26 non-ICVD patients were used as controls.The points of electro-acupuncture wereQuchi(LI 12),Waiguan(SJ 5)and Huantiao(GB 30)and Zusanli(St 36).After a courseof treatment,the SS levels in plasma and CSF were significantly increased in the patientsof group 1 with good result and their plasma PP level had no significant change.In thepatients with poor result,however,the PP level was significantly decreased.The resultssuggested that electro-acupuncture might play an active role in alleviating the SSmetabolic disturbance in CNS of ICVD patients. 展开更多
关键词 ELECTROACUPUNCTURE ADULT Aged Cerebral Infarction FEMALE Humans Intracranial Embolism and Thrombosis Male Middle Aged Pancreatic Polypeptide SOMATOSTATIN
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Small gastrointestinal stromal tumor concomitant with early gastric cancer:A case report 被引量:18
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作者 Ying-Lung Lin Jeh-En Tzeng Chih-Wen Lin 《World Journal of Gastroenterology》 SCIE CAS CSCD 2006年第5期815-817,共3页
The term gastrointestinal stromal tumors (GISTs) is defined diagnostically as the main group of mesenchymal tumors with spindle or epithelioid cells arising from the wall of the gastrointestinal tract with immunohis... The term gastrointestinal stromal tumors (GISTs) is defined diagnostically as the main group of mesenchymal tumors with spindle or epithelioid cells arising from the wall of the gastrointestinal tract with immunohistochemical reactivity for CD117 antibody. Previous studies revealed that cells in GISTs express a growth factor receptor with tyrosine kinase activity (termed c-kit), which is the product of the c-kit protooncogene. The most specific and practical diagnostic criteria for GISTs are: immunohistochemically determined c-kit (CD117) expression; mitotic score; and tumor size. A small GIST concomitant with early gastric cancer is rarely encountered clinically. Herein we have reported a case of a 1.1-cm GIST detected by esophagogastroduo denoscopy concomitant with a IIc type of early gastric cancer (signet ring cell type). It was detected during a routine physical health examination. To our knowledge, this is the first report of a small GIST concomitant with a signet ring cell type of early gastric cancer. 展开更多
关键词 Gastrointestinal stromal tumor Early gastriccancer Npylori infection Biopsy urease test CD117
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Progastrin-releasing peptide and gastrin-releasing peptide receptor mRNA expression in non-tumor tissues of the human gastrointestinal tract 被引量:7
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作者 Hans-Jürg Monstein Niclas Grahn +1 位作者 Mikael Truedsson Bodil Ohisson 《World Journal of Gastroenterology》 SCIE CAS CSCD 2006年第16期2574-2578,共5页
AIM: To investigate the expression of gastrin-releasing peptide (GRP) and GRP-receptor mRNA in non-tumor tissues of the human esophagus, gastrointestinal tract, pancreas and gallbladder using molecular biology tech... AIM: To investigate the expression of gastrin-releasing peptide (GRP) and GRP-receptor mRNA in non-tumor tissues of the human esophagus, gastrointestinal tract, pancreas and gallbladder using molecular biology techniques. METHODS: Poly A^+ mRNA was isolated from total RNA extracts using an automated nucleic acid extractor and, subsequently, converted into single-stranded cDNA (sscDNA). PCR amplifications were carried out using genespecific GRP and GRP-receptor primers. The specificity of the PCR amplicons was further confirmed by Southern blot analyses using gene-specific GRP and GRP-receptor hybridization probes. RESULTS: Expression of GRP and GRP-receptor mRNA was detected at various levels in nearly all segments of the non-tumor specimens analysed, except the gallbladder. In most of the biopsy specimens, coexpression of both GRP and GRP-receptor mRNA appeared to take place. However, expression of GRP mRNA was more prominent than was GRP-receptor mRNA. CONCLUSION: GRP and GRP-receptor mRNAs are expressed throughout the gastrointestinal tract and provides information for the future mapping and determination of its physiological importance in normal and tumor cells. 展开更多
关键词 Gastrin releasing peptide (GRP) Gastrinreleasing peptide receptor (GRPR) mRNA expression MORPHOGENESIS Gastrointestinal tract
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Infectious causation of chronic disease:Examining the relationship between Giardia lamblia infection and irritable bowel syndrome 被引量:3
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作者 Alice S Penrose Eden V Wells Allison E Aiello 《World Journal of Gastroenterology》 SCIE CAS CSCD 2007年第34期4574-4578,共5页
AIM: To evaluate whether a higher prevalence of Giardia lamblia infection is associated with an increase in irritable bowel syndrome (IBS) prescriptions at the county level in Michigan. METHODS: The Michigan Disease S... AIM: To evaluate whether a higher prevalence of Giardia lamblia infection is associated with an increase in irritable bowel syndrome (IBS) prescriptions at the county level in Michigan. METHODS: The Michigan Disease Surveillance System (MDSS) was used to ascertain both the numbers of Giardia lamblia infections as well as the total number of foodborne illnesses per population by county in Michigan during 2005. This was compared with Blue Cross Blue Shield (BCBS) of Michigan numbers of drug prescriptions for IBS per one thousand members per county in 2005. These data were also analyzed for associations with per capita income by county and the number of refugees entering each county in 2005. RESULTS: There were a total of 786 confirmed cases of Giardia lamblia reported to MDSS in 2005. During the same time period, the number of prescriptions for IBS varied from 0.5 per 1000 members up to 6.0 per 1000 members per month. There was no trend towards higher numbers of IBS prescriptions in the counties with more Giardia lamblia infections. Per capita income was not associated with either IBS prescriptions or Giardiasis. There was a significant linear association between the number of refugees entering each county, and the number of Giardia lamblia cases per 100 000 population. CONCLUSION: In this ecological study, there was no association found between BCBS prescriptions for IBS and Giardia lamblia infections in Michigan counties. Our findings may have been influenced by the disparate number of refugees admitted per county. 展开更多
关键词 FOODBORNE GASTROINTESTINAL Giardia lamblia Irritable bowel syndrome PARASITE Prescription Refugees
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Presence of CCK-A, B receptors and effect of gastrin and cholecystokinin on growth of pancreatobiliary cancer cell lines 被引量:4
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作者 Jin-Young Jang Sun-Whe Kim +2 位作者 Ja-Lok Ku Yong-Hyun Park Jae-Gahb Park 《World Journal of Gastroenterology》 SCIE CAS CSCD 2005年第6期803-809,共7页
AIM: To investigate the effects of gastrin and cholecystokinin (CCK) and their specific antagonists on the growth of pancreatic and biliary tract cancer cell lines. METHODS: Five pancreatic and 6 biliary cancer cell l... AIM: To investigate the effects of gastrin and cholecystokinin (CCK) and their specific antagonists on the growth of pancreatic and biliary tract cancer cell lines. METHODS: Five pancreatic and 6 biliary cancer cell lines with 2 conrtol cells were used in this study. Cell proliferation study was done using 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) test and direct cell count method. Reverse transcription-polymerase chain reaction (RT-PCR) and slot blot hybridization were performed to examine and quantify the expression of hormonal receptors in these cell lines. RESULTS: SNU-308 showed a growth stimulating effect by gastrin-17, as did SNU-478 by both gastrin-17 and CCK-8. The trophic effect of these two hormones was completely blocked by specific antagonists (L-365, 260 for gastrin and L-364, 718 for CCK). Other cell lines did not respond to gastrin or CCK. In RT-PCR, the presence of CCK-A receptor and CCK-B/gastrin receptor mRNA was detected in all biliary and pancreatic cancer cell lines. In slot blot hybridization, compared to the cell lines which did not respond to hormones, those that responded to hormones showed high expression of receptor mRNA. CONCLUSION: Gastrin and CCK exert a trophic action on some of the biliary tract cancers. 展开更多
关键词 Bile duct cancer Gallbladder cancer Pancreatic cancer GASTRIN CHOLECYSTOKININ
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