模拟胃肠道消化液中小球藻类金属硫蛋白对镉离子的结合作用研究

利用锌诱导小球藻,通过提取分离和纯化,获得锌结合类金属硫蛋白(MT-like蛋白)。通过透析过程,研究模拟胃、肠道消化液中MT-like蛋白对Cd^(2+)的结合作用。结果表明,在模拟胃液中MT-like蛋白处于脱金属状态,与Cd^(2+)的结合能力弱。而在肠消化液中,MT-like蛋白能与Cd^(2+)结合,20 mg·L^(-1) MT-like蛋白与100μg·L^(-1) Cd^(2+)的结合率可达46.0%。研究发现,在模拟胃、肠液中胃蛋白酶和胰蛋白酶都会降低MT-like蛋白对Cd^2+的结合能力。研究结果对进一步开发MT-like蛋白作为Cd^(2+)的生物解毒剂具有重要意义。

破壁对西藏高原油菜蜂花粉胃肠道消化吸收特性的影响

实验以西藏高原油菜蜂花粉为原料,以破壁率为筛选指标,得出油菜蜂花粉在株磨仪中的最适添加量为4~8 g,此条件下的油菜蜂花粉的破壁率接近100%。以内容物浸出率、可溶性蛋白和多糖为检测指标,以4 g油菜蜂花粉添加量为基准,研究株磨仪研磨破碎前后油菜蜂花粉在模拟胃液、肠液和胃肠液中消化吸收特性。实验结果表明:油菜蜂花粉无需破壁就可以通过萌发孔释放内容物和其中的营养物质,即油菜蜂花粉无需破壁,就可以直接食用。

The effect of simulated gastrointestinal digestion on shrimp Penaeus vannamei allergenicity

Pen a 1 is a major shrimp allergen that induces an allergy. The aim of this study is to assess the effect of simulated gastrointestinal fluids on the allergenicity of Pen a 1. Purified Pen a 1 from shrimp （Penaeus vannamei） was subjected to digestion in SGF （simulated gastric fluid） and SIF （simulated intestinal fluid） for a set time. The allergenicity of digestive Pen a 1 was analyzed by immunoblotting and Ci-ELISA, using pool sera from patients with shrimp specific IgE. The results showed that Pen a 1 exhibited a decrease in allergenicity with increasing digestion time in the SGF and SIF. However, Pen a 1 exhibited strong resistance to digestive fluids, and all yielded fragments （33 kD, 23 kD, and 14 kD） showed allergic activity. Therefore, anti-digestion may be an important factor for Pen a 1 to induce an allergy.