目的阐明胃脂酶(LIPF)在胃癌细胞增殖、迁移和侵袭中的作用及其对丝裂原活化蛋白激酶(MAPK)信号通路的影响。方法采用GEPIA和Human Protein Altas评估胃癌组织中LIPF的表达。实时定量PCR(qPCR)和蛋白印迹(Western blot)检测LIPF在胃癌细...目的阐明胃脂酶(LIPF)在胃癌细胞增殖、迁移和侵袭中的作用及其对丝裂原活化蛋白激酶(MAPK)信号通路的影响。方法采用GEPIA和Human Protein Altas评估胃癌组织中LIPF的表达。实时定量PCR(qPCR)和蛋白印迹(Western blot)检测LIPF在胃癌细胞SGC-7901中的表达。SGC-7901细胞分为阴性对照组、LIPF过表达组和LIPF过表达+MAPK激活剂组。通过MTT、划痕实验和Transwell实验分析SGC-7901细胞中的细胞活力、迁移和侵袭能力。qPCR和Western blot用于检测细胞周期蛋白依赖性激酶4(CDK4)、基质金属蛋白酶9(MMP9)、磷酸化p38(p-p38)和磷酸化c-Jun氨基末端激酶(p-JNK)的表达。结果LIPF在胃癌组织和SGC-7901细胞中表达降低(P<0.05)。与阴性对照组相比,LIPF过表达组的细胞增殖活力、划痕愈合率和侵袭细胞数均减少且CDK4、MMP9、p-p38和-JNK水平降低(P<0.05)。此外,与LIPF过表达组相比,LIPF过表达+MAPK激活剂组的细胞增殖活力、划痕愈合率和侵袭细胞数增加,且CDK4、MMP9、p-p38和-JNK水平升高(P<0.05)。结论LIPF可通过体外调控p38 MAPK信号通路抑制胃癌的增殖、迁移和侵袭,可能为胃癌患者提供新的治疗干预措施。展开更多
AIM: To understand the correlation of serum cholinesterase (CHE) activity with gastric cancer and to assess their clinical significance.METHODS: The velocity method was adopted to detect the activity of serum CHE in p...AIM: To understand the correlation of serum cholinesterase (CHE) activity with gastric cancer and to assess their clinical significance.METHODS: The velocity method was adopted to detect the activity of serum CHE in patients with gastric cancer and in patients with non-malignant tumor as controls.RESULTS: The serum CHE activity in the treatment group was significantly lower than that in the control group with a very significant difference between the two groups (83.3:113.1,P = 0.0003). Age was significantly associated with the incidence of gastric caner.CONCLUSION: Serum CHE activity has a close relation with the incidence of gastric cancer.展开更多
AIM: To study the effect of bromophenacyl bromide (BPB), a phospholipase A2 inhibitor on gastric secretion and to protect chemically induced gastric and duodenal ulcers in rats. METHODS: Acid secretion studies were un...AIM: To study the effect of bromophenacyl bromide (BPB), a phospholipase A2 inhibitor on gastric secretion and to protect chemically induced gastric and duodenal ulcers in rats. METHODS: Acid secretion studies were undertaken in pylorus-ligated rats with BPB treatment (0, 5, 15 and 45 mg/kg). Gastric and duodenal lesions in the rats were induced by ethanol and cysteamine respectively. The levels of gastric wall mucus, nonprotein sulfhydryls (NP- SH) and myeloperoxidase (MPO) were also measured in the glandular stomach of rats following ethanol induced gastric lesions. RESULTS: BPB produced a dose-dependent inhibition of gastric acid secretion and acidity in rats. Pretreatment with BPB significantly attenuated the formation of etha- nol induced gastric lesion. BPB also protected intestinal mucosa against cysteamine-induced duodenal ulcers. The antiulcer activity of BPB was associated with signifi- cant inhibition of ethanol-induced depletion of gastric wall mucus, NP-SH and MPO. These findings pointed towards the mediation of sulfhydryls in BPB induced gas- trointestinal cytoprotection. CONCLUSION: BPB possesses significant antiulcer and cytoprotective activity against experimentally induced gastroduodenal lesions.展开更多
文摘AIM: To understand the correlation of serum cholinesterase (CHE) activity with gastric cancer and to assess their clinical significance.METHODS: The velocity method was adopted to detect the activity of serum CHE in patients with gastric cancer and in patients with non-malignant tumor as controls.RESULTS: The serum CHE activity in the treatment group was significantly lower than that in the control group with a very significant difference between the two groups (83.3:113.1,P = 0.0003). Age was significantly associated with the incidence of gastric caner.CONCLUSION: Serum CHE activity has a close relation with the incidence of gastric cancer.
文摘AIM: To study the effect of bromophenacyl bromide (BPB), a phospholipase A2 inhibitor on gastric secretion and to protect chemically induced gastric and duodenal ulcers in rats. METHODS: Acid secretion studies were undertaken in pylorus-ligated rats with BPB treatment (0, 5, 15 and 45 mg/kg). Gastric and duodenal lesions in the rats were induced by ethanol and cysteamine respectively. The levels of gastric wall mucus, nonprotein sulfhydryls (NP- SH) and myeloperoxidase (MPO) were also measured in the glandular stomach of rats following ethanol induced gastric lesions. RESULTS: BPB produced a dose-dependent inhibition of gastric acid secretion and acidity in rats. Pretreatment with BPB significantly attenuated the formation of etha- nol induced gastric lesion. BPB also protected intestinal mucosa against cysteamine-induced duodenal ulcers. The antiulcer activity of BPB was associated with signifi- cant inhibition of ethanol-induced depletion of gastric wall mucus, NP-SH and MPO. These findings pointed towards the mediation of sulfhydryls in BPB induced gas- trointestinal cytoprotection. CONCLUSION: BPB possesses significant antiulcer and cytoprotective activity against experimentally induced gastroduodenal lesions.
