借鉴分类问题的算法,推广到回归问题中去,针对用于分类问题的SOR(successive overrelaxation for support vector)支持向量机算法,提出SORR(successive overrelaxation for support vector regression)支持向量回归算法,并应用于医学上...借鉴分类问题的算法,推广到回归问题中去,针对用于分类问题的SOR(successive overrelaxation for support vector)支持向量机算法,提出SORR(successive overrelaxation for support vector regression)支持向量回归算法,并应用于医学上三类血浆脂蛋白(VLDL、LDL、HDL)测定样本中胆固醇的含量。数值实验表明:SORR算法有效,与标准的支持向量回归SVR算法相比,保持了相同的回归精度,提高了学习速度,为临床上测定胆固醇含量提供新的有效方法。展开更多
Cholesterol esterase (Cease) from bovine pancreas and cholesterol oxidasc (COD) from Bravibacterium recombinant type have been innobilized individually and co-immobilized onto arylamine glass (pore diameter 55nm) thro...Cholesterol esterase (Cease) from bovine pancreas and cholesterol oxidasc (COD) from Bravibacterium recombinant type have been innobilized individually and co-immobilized onto arylamine glass (pore diameter 55nm) through the process of diazotization. Cease and COD retained 92.65% and 85.54% of the initial activity with conjugation yields of 7.2 mg/g and 8.3mg/g support respectively when imnobilized individually on arylamine glass beads, but retained 89.58% of the initial activity with a conjugation yield of 2.9 mg/g support when co-immobilized on the same support. The effects of pH, temperature, time of incubation, substrate concentration, serum inorganic salts & metabolites, thermal stability, storage stability in cold and reusability on the immobilized enzymes were studied and compared with those of free enzymes. The analytic use of both individually immobilized and co-immobilized enzymes in discrete analysis of total and free cholesterol in serum is demonstrated.展开更多
文摘借鉴分类问题的算法,推广到回归问题中去,针对用于分类问题的SOR(successive overrelaxation for support vector)支持向量机算法,提出SORR(successive overrelaxation for support vector regression)支持向量回归算法,并应用于医学上三类血浆脂蛋白(VLDL、LDL、HDL)测定样本中胆固醇的含量。数值实验表明:SORR算法有效,与标准的支持向量回归SVR算法相比,保持了相同的回归精度,提高了学习速度,为临床上测定胆固醇含量提供新的有效方法。
文摘Cholesterol esterase (Cease) from bovine pancreas and cholesterol oxidasc (COD) from Bravibacterium recombinant type have been innobilized individually and co-immobilized onto arylamine glass (pore diameter 55nm) through the process of diazotization. Cease and COD retained 92.65% and 85.54% of the initial activity with conjugation yields of 7.2 mg/g and 8.3mg/g support respectively when imnobilized individually on arylamine glass beads, but retained 89.58% of the initial activity with a conjugation yield of 2.9 mg/g support when co-immobilized on the same support. The effects of pH, temperature, time of incubation, substrate concentration, serum inorganic salts & metabolites, thermal stability, storage stability in cold and reusability on the immobilized enzymes were studied and compared with those of free enzymes. The analytic use of both individually immobilized and co-immobilized enzymes in discrete analysis of total and free cholesterol in serum is demonstrated.
