目的:探讨七氟醚对基底前脑胆碱能系统和认知功能的影响。方法:老年C57小鼠吸入七氟醚建立全身麻醉动物模型,利用Morris水迷宫检测认知功能,免疫荧光检测Meynert基底核中胆碱能神经元计数,Western Blot检测额叶皮层胆碱乙酰转移酶及高...目的:探讨七氟醚对基底前脑胆碱能系统和认知功能的影响。方法:老年C57小鼠吸入七氟醚建立全身麻醉动物模型,利用Morris水迷宫检测认知功能,免疫荧光检测Meynert基底核中胆碱能神经元计数,Western Blot检测额叶皮层胆碱乙酰转移酶及高亲和力胆碱转运体的蛋白水平,ELISA测定额叶皮层胆碱乙酰转移酶的活性。结果:与对照组相比,七氟醚组小鼠平台潜伏期延长,平台穿越次数减少(2.1±0.4 vs 5.6±0.5,P<0.05),原平台所在象限停留时间缩短[(21.5±2.4)%vs(48.6±2.8)%,P<0.05]。此外,七氟醚组小鼠Meynert基底核胆碱能神经元计数减少(3748±248 vs 5942±315,P<0.05),额叶皮层胆碱乙酰转移酶及高亲和力胆碱转运体表达下降,伴有胆碱乙酰转移酶活性降低[(31.4±1.1)μmol/(g.h)vs(55.8±1.3)μmol/(g.h),P<0.05]。结论:七氟醚可损害老龄鼠基底前脑胆碱能系统并诱导认知功能损害。展开更多
Diacetylenic glycerophosphatidylhydroxyethanol was synthesized from diacetylenic glycerophosphatidylcholine in the presence of phospholipase D from peanut seeds. The product structure was characterized by elemental an...Diacetylenic glycerophosphatidylhydroxyethanol was synthesized from diacetylenic glycerophosphatidylcholine in the presence of phospholipase D from peanut seeds. The product structure was characterized by elemental analysis, IR, 1H NMR and 13C NMR. The effects of the reaction conditions on the enzyme-catalyzed reaction are discussed. The results show that the enzyme （activity） was greatly increased by adding SDS. The yield of the reaction was increased compared with that reported in the literature under the conditions of pH=5.6, temperature 30 ℃, Ca2+ 40mmol/L, （n（substrate ）∶）n（SDS）=（2∶1）, reaction time 8 h. The cost was lower. The melting point of the product was 69.5 ℃.展开更多
文摘目的:探讨七氟醚对基底前脑胆碱能系统和认知功能的影响。方法:老年C57小鼠吸入七氟醚建立全身麻醉动物模型,利用Morris水迷宫检测认知功能,免疫荧光检测Meynert基底核中胆碱能神经元计数,Western Blot检测额叶皮层胆碱乙酰转移酶及高亲和力胆碱转运体的蛋白水平,ELISA测定额叶皮层胆碱乙酰转移酶的活性。结果:与对照组相比,七氟醚组小鼠平台潜伏期延长,平台穿越次数减少(2.1±0.4 vs 5.6±0.5,P<0.05),原平台所在象限停留时间缩短[(21.5±2.4)%vs(48.6±2.8)%,P<0.05]。此外,七氟醚组小鼠Meynert基底核胆碱能神经元计数减少(3748±248 vs 5942±315,P<0.05),额叶皮层胆碱乙酰转移酶及高亲和力胆碱转运体表达下降,伴有胆碱乙酰转移酶活性降低[(31.4±1.1)μmol/(g.h)vs(55.8±1.3)μmol/(g.h),P<0.05]。结论:七氟醚可损害老龄鼠基底前脑胆碱能系统并诱导认知功能损害。
文摘Diacetylenic glycerophosphatidylhydroxyethanol was synthesized from diacetylenic glycerophosphatidylcholine in the presence of phospholipase D from peanut seeds. The product structure was characterized by elemental analysis, IR, 1H NMR and 13C NMR. The effects of the reaction conditions on the enzyme-catalyzed reaction are discussed. The results show that the enzyme （activity） was greatly increased by adding SDS. The yield of the reaction was increased compared with that reported in the literature under the conditions of pH=5.6, temperature 30 ℃, Ca2+ 40mmol/L, （n（substrate ）∶）n（SDS）=（2∶1）, reaction time 8 h. The cost was lower. The melting point of the product was 69.5 ℃.
