目的:分析探讨多层螺旋CT联合肿瘤标志物(CEA、CA19-9和AFP)水平对肝内周围型胆管细胞癌(IHPCC)的诊断价值。方法:从2015年7月—2019年7月于本院接诊的疑诊IHPCC患者筛选出89例,依据病理结果分为IHPCC组(52例)、非IHPCC组(37例),比较MSC...目的:分析探讨多层螺旋CT联合肿瘤标志物(CEA、CA19-9和AFP)水平对肝内周围型胆管细胞癌(IHPCC)的诊断价值。方法:从2015年7月—2019年7月于本院接诊的疑诊IHPCC患者筛选出89例,依据病理结果分为IHPCC组(52例)、非IHPCC组(37例),比较MSCT联合肿瘤标志物与单独CT检查对IHPCC的诊断效能。结果:IPHCC组CEA(11.37±2.56 vs 3.12±1.64)、CA19-9(44.58±3.89 vs 19.76±4.68)、AFP(20.51±5.83 vs 6.42±1.22)明显高于非IHPCC组(P<0.05)。MSCT联合肿瘤标志物诊断敏感度(94.23% vs 73.08%)、特异度(94.59% vs 72.97%)、阳性预测值(96.08% vs 79.17%)、阴性预测值(92.11% vs 65.85%)以及准确度(94.38% vs 73.03%)均明显高于单独CT诊断(P<0.05)。结论:相较于单独CT检查,MSCT联合肿瘤标志物(CEA、CA19-9、AFP)水平检查对IHPCC诊断效能更高,更具诊断价值。展开更多
目的探讨肝癌衍生生长因子(HDGF)与原发性肝内胆管细胞癌(PICC)预后的关系。方法选择2011年1月至2014年12月共55例肝内胆管细胞癌手术标本和30例非癌变标本,进行免疫组织化学(IHC)染色及评估、全RNA提取、逆转录PCR(RT-PCR)以及结果判...目的探讨肝癌衍生生长因子(HDGF)与原发性肝内胆管细胞癌(PICC)预后的关系。方法选择2011年1月至2014年12月共55例肝内胆管细胞癌手术标本和30例非癌变标本,进行免疫组织化学(IHC)染色及评估、全RNA提取、逆转录PCR(RT-PCR)以及结果判定。结果特异性HDGF染色主要存在于良性和恶性上皮细胞的细胞核和细胞质中。52.7%(29/55)的PICC提示细胞核HDGF EI 2级。对比之下,非癌变标本的细胞核HDGF蛋白表达EI 2级率是33.3%(10/30)。核HDGF表达与PICC的T分类、N分类和肿瘤分化密切相关(P<0.01)。Log-rank检验显示,在PICC中高HDGF表达组生存期短,而低HDGF表达组生存期长。结论 PICC中核HDGF水平升高,并与PICC的TNM分期、肿瘤分化、PICC的生存期有关。HDGF可能是PICC预后的独立预测因素。展开更多
Objective To investigate and compare the biological characteristics and sensitivity to chemotherapy and radiotherapy of intrahepatic and extrahepatic cholangiocarcinoma cells in vitro.Methods The intrahepatic and extr...Objective To investigate and compare the biological characteristics and sensitivity to chemotherapy and radiotherapy of intrahepatic and extrahepatic cholangiocarcinoma cells in vitro.Methods The intrahepatic and extrahepatic cholangiocarcinoma cell lines were established,and cells with steady passage were chosen to study the biological characteristics including morphology,growth dynamics,chromosome,and levels of cancer antigen(CA)125,CA19-9,alpha-fetoprotein(AFP),and carcino-embryonic antigen(CEA).Meanwhile,MTT assay was used to determine the sensitivity of both kinds of cells to 6 chemotherapeutic drugs,including cisplatin,paclitaxel,harringtonine,5-fluorouracil,vincristine,and aclacimomycin,and the inhibitory rate of cells under the irradiation of 10 Gy ray was also measured.Results The intrahepatic cholangiocarcinoma cells were mostly fusiform in shape,and extrahepatic cholangiocarcinoma cells were mostly round or polygon in shape.Their doubling time was 26.3 hours and 23.1 hours,respectively.Their average number of chromosomes was 59(range,38-84)and 67(range,49-103),respectively.The chromosome karyotypes of most intrahepatic cholangiocarcinoma cells were hyperdiploid and hypotriploid,while hypertriploid was predominant in extrahepatic cholangiocarcinoma cells.The level of CA 125 in supernatant of extrahepatic cholangiocarcinoma cells increased obviously,while levels of other determined tumor markers in both kinds of cells were all within normal range.The intrahepatic cholangiocarcinoma cells were low sensitive to cisplatin and paclitaxel,but not sensitive to the other 4 chemotherapeutic drugs.The extrahepatic cholangiocarcinoma cells were high sensitive to cisplatin,but not sensitive to the other 5 drugs.Both kinds of cells had poor sensitivity to radiotherapy.Conclusions Intrahepatic and extrahepatic cholangiocarcinoma cells show differences in shape,doubling time,chromosome karyotype,tumor marker level,and chemosensitivity,whereas they both have poor radiosensitivity.Though they are similar in histopathology,they have different growth characteristics and have discrepancy in treatment and prognosis.展开更多
文摘目的:分析探讨多层螺旋CT联合肿瘤标志物(CEA、CA19-9和AFP)水平对肝内周围型胆管细胞癌(IHPCC)的诊断价值。方法:从2015年7月—2019年7月于本院接诊的疑诊IHPCC患者筛选出89例,依据病理结果分为IHPCC组(52例)、非IHPCC组(37例),比较MSCT联合肿瘤标志物与单独CT检查对IHPCC的诊断效能。结果:IPHCC组CEA(11.37±2.56 vs 3.12±1.64)、CA19-9(44.58±3.89 vs 19.76±4.68)、AFP(20.51±5.83 vs 6.42±1.22)明显高于非IHPCC组(P<0.05)。MSCT联合肿瘤标志物诊断敏感度(94.23% vs 73.08%)、特异度(94.59% vs 72.97%)、阳性预测值(96.08% vs 79.17%)、阴性预测值(92.11% vs 65.85%)以及准确度(94.38% vs 73.03%)均明显高于单独CT诊断(P<0.05)。结论:相较于单独CT检查,MSCT联合肿瘤标志物(CEA、CA19-9、AFP)水平检查对IHPCC诊断效能更高,更具诊断价值。
文摘目的探讨肝癌衍生生长因子(HDGF)与原发性肝内胆管细胞癌(PICC)预后的关系。方法选择2011年1月至2014年12月共55例肝内胆管细胞癌手术标本和30例非癌变标本,进行免疫组织化学(IHC)染色及评估、全RNA提取、逆转录PCR(RT-PCR)以及结果判定。结果特异性HDGF染色主要存在于良性和恶性上皮细胞的细胞核和细胞质中。52.7%(29/55)的PICC提示细胞核HDGF EI 2级。对比之下,非癌变标本的细胞核HDGF蛋白表达EI 2级率是33.3%(10/30)。核HDGF表达与PICC的T分类、N分类和肿瘤分化密切相关(P<0.01)。Log-rank检验显示,在PICC中高HDGF表达组生存期短,而低HDGF表达组生存期长。结论 PICC中核HDGF水平升高,并与PICC的TNM分期、肿瘤分化、PICC的生存期有关。HDGF可能是PICC预后的独立预测因素。
文摘Objective To investigate and compare the biological characteristics and sensitivity to chemotherapy and radiotherapy of intrahepatic and extrahepatic cholangiocarcinoma cells in vitro.Methods The intrahepatic and extrahepatic cholangiocarcinoma cell lines were established,and cells with steady passage were chosen to study the biological characteristics including morphology,growth dynamics,chromosome,and levels of cancer antigen(CA)125,CA19-9,alpha-fetoprotein(AFP),and carcino-embryonic antigen(CEA).Meanwhile,MTT assay was used to determine the sensitivity of both kinds of cells to 6 chemotherapeutic drugs,including cisplatin,paclitaxel,harringtonine,5-fluorouracil,vincristine,and aclacimomycin,and the inhibitory rate of cells under the irradiation of 10 Gy ray was also measured.Results The intrahepatic cholangiocarcinoma cells were mostly fusiform in shape,and extrahepatic cholangiocarcinoma cells were mostly round or polygon in shape.Their doubling time was 26.3 hours and 23.1 hours,respectively.Their average number of chromosomes was 59(range,38-84)and 67(range,49-103),respectively.The chromosome karyotypes of most intrahepatic cholangiocarcinoma cells were hyperdiploid and hypotriploid,while hypertriploid was predominant in extrahepatic cholangiocarcinoma cells.The level of CA 125 in supernatant of extrahepatic cholangiocarcinoma cells increased obviously,while levels of other determined tumor markers in both kinds of cells were all within normal range.The intrahepatic cholangiocarcinoma cells were low sensitive to cisplatin and paclitaxel,but not sensitive to the other 4 chemotherapeutic drugs.The extrahepatic cholangiocarcinoma cells were high sensitive to cisplatin,but not sensitive to the other 5 drugs.Both kinds of cells had poor sensitivity to radiotherapy.Conclusions Intrahepatic and extrahepatic cholangiocarcinoma cells show differences in shape,doubling time,chromosome karyotype,tumor marker level,and chemosensitivity,whereas they both have poor radiosensitivity.Though they are similar in histopathology,they have different growth characteristics and have discrepancy in treatment and prognosis.
