Effect of Transgenic Bt plus CpTI Cotton on Carboxylesterase and Acetylcholinesterase of Cotton Aphid Aphis gossypii

[Objective] The research aimed to assess the effect of transgenic Bt plus CpTI cotton variety SGK321 on carboxylesterase and acetylcholinesterase of cotton aphid Aphis gossypii and provide theoretical basis for studying the biosafety of transgenic cotton.[Method] Cotton aphids were fed with SGK321 and Shiyuan321（normal parental varieties） for over 40 generations.Enzyme activities were compared between cotton aphids feeding on SGK321 for 1,2,3,41,42 and 43 generations with those on Shiyuan321.[Result] The carboxylesterase activity of cotton aphids feeding on SGK321 for 1 generation was significantly higher than those feeding on Shiyuan321.Acetylcholinesterase activity of cotton aphids feeding on SGK321 for 1,2 and 3 generations were significantly higher than those feeding on Shiyuan321 in the same generation.But there was no significant difference of enzyme activity between cotton aphids feeding on SGK321 for a long term and those feeding on parental cotton.[Conclusion] The cotton aphid that feeding on transgenic Bt plus CpTI cotton SGK321 for a long time has adaptivity to SGK321 by regulating the detoxifying enzyme.

戊乙奎醚与血液净化治疗有机磷中毒的效果

目的探讨急性有机磷农药中毒(AOPP)患者应用戊乙奎醚与血液净化治疗的效果。方法76例研究对象均为2019年1月~2020年12月菏泽市立医院收治的AOPP患者,以随机数表法划分为两组。两组均给予常规治疗。对照组38例应用血液净化治疗,研究组38例在此基础上应用戊乙奎醚治疗。比较两组并发症发生率,以及治疗前后胆碱酯酶(chE)水平、心肌肌钙蛋白Ⅰ(cTnI)与血清肌酸激酶同工酶-MB(CK-MB)。结果在并发症发生率比较中,研究组较对照组更低(P<0.05)。治疗后,研究组存活者chE水平较对照组更高(P<0.01),CK-MB、cTnI水平较对照组更低(P<0.01)。结论戊乙奎醚与血液净化联合治疗AOPP效果确切,值得临床应用。