期刊文献+
共找到15篇文章
< 1 >
每页显示 20 50 100
非繁殖季节胚胎因素对绵羊胚胎移植影响的研究 被引量:3
1
作者 王素梅 亓美玉 +2 位作者 丁威 谢小来 李武 《中国畜牧兽医》 CAS 2005年第7期30-31,共2页
在非繁殖季节(6月份)利用进口绵羊胚胎,手术法移植。通过胚胎类型、冷冻胚胎解冻后停留时间以及胚胎发育阶段对绵羊胚胎移植效果的分析。得出以下结论:1鲜胚移植妊娠率和产羔率显著高于冻胚:鲜胚为61.11%(11/18)和50.00%(9/18),冻胚为48... 在非繁殖季节(6月份)利用进口绵羊胚胎,手术法移植。通过胚胎类型、冷冻胚胎解冻后停留时间以及胚胎发育阶段对绵羊胚胎移植效果的分析。得出以下结论:1鲜胚移植妊娠率和产羔率显著高于冻胚:鲜胚为61.11%(11/18)和50.00%(9/18),冻胚为48.11%(153/318)和46.86%(149/318);2随冷冻胚胎解冻后停留时间的延长胚胎移植效果有升高的趋势;3从桑椹胚到囊胚时期胚胎发育阶段对胚胎移植效果没有明显影响。 展开更多
关键词 非繁殖季节 胚胎因素 绵羊 胚胎移植
下载PDF
胚胎因素对奶牛体外受精胚胎解冻孵化影响的试验研究 被引量:2
2
作者 佟桂芝 王洪宝 +3 位作者 董正德 韩永胜 丁丽艳 李同豹 《中国奶牛》 2007年第6期25-27,共3页
对胚胎因素影响奶牛体外受精胚胎培养效果进行了研究。结果发现:在胚胎因素中,胚胎类型(冻胚和鲜胚)与胚胎发育阶段对胚胎培养效果没有显著影响(P>0.05);冷冻胚胎解冻后停留时间对胚胎培养效果影响显著(P<0.01);冷冻胚胎解冻后胚... 对胚胎因素影响奶牛体外受精胚胎培养效果进行了研究。结果发现:在胚胎因素中,胚胎类型(冻胚和鲜胚)与胚胎发育阶段对胚胎培养效果没有显著影响(P>0.05);冷冻胚胎解冻后停留时间对胚胎培养效果影响显著(P<0.01);冷冻胚胎解冻后胚胎等级对胚胎培养效果影响显著(P<0.05),解冻后A级胚胎存活率(80%,128/160)、囊胚孵化率(58.75%,94/160)显著高于B级胚胎存活率(59.38%,95/160)和囊胚孵化率(37.5%,60/160)(P<0.05);解冻水浴温度(20℃、30℃)对培养效果有极显著影响(P<0.01)。 展开更多
关键词 奶牛 体外受精胚胎 胚胎因素 胚胎存活率 囊胚孵化率
下载PDF
反复种植失败的胚胎因素及对策 被引量:2
3
作者 李为玉 段金良 曾琼芳 《中国性科学》 2021年第5期64-66,共3页
反复种植失败是指移植了多个优质胚胎却仍未获得胚胎种植,影响胚胎种植的重要因素包括胚胎自身和子宫内膜,同时这两个重要因素的协调对话也起至关重要的作用。本研究将从胚胎因素探讨其在反复种植失败中扮演的角色,并从卵子质量、精子DN... 反复种植失败是指移植了多个优质胚胎却仍未获得胚胎种植,影响胚胎种植的重要因素包括胚胎自身和子宫内膜,同时这两个重要因素的协调对话也起至关重要的作用。本研究将从胚胎因素探讨其在反复种植失败中扮演的角色,并从卵子质量、精子DNA完整性、胚胎染色体异常、胚胎透明带硬化以及胚胎培养条件等方面进行综述。 展开更多
关键词 反复种植失败 卵子质量 胚胎因素 染色体异常
下载PDF
胚胎因素对肉羊胚胎移植效果的影响
4
作者 阿曼开迪·莫哈麦提汗 《甘肃畜牧兽医》 2016年第20期85-86,共2页
目的:观察分析胚胎因素对肉羊胚胎移植效果的影响。方法:选择258只已产1胎营养水平中上等的受体母肉羊为研究对象,对其移植A级胚胎,对比胚胎因素对肉羊胚胎移植效果的影响。结果:移植1枚桑椹胚的受胎率显著高于移植1枚囊胚者,不... 目的:观察分析胚胎因素对肉羊胚胎移植效果的影响。方法:选择258只已产1胎营养水平中上等的受体母肉羊为研究对象,对其移植A级胚胎,对比胚胎因素对肉羊胚胎移植效果的影响。结果:移植1枚桑椹胚的受胎率显著高于移植1枚囊胚者,不同体外保存时间的受体羊受胎率差异不具有统计学意义(P〉0.05),随着胚胎移植数量的增长,受胎率明显提高(P〈0.05)。结论:胚胎因素与肉羊胚胎移植效果密切相关,其中胚胎发育阶段、移植胚胎数对受胎率有显著影响,胚胎体外保存时间对受胎率无显著影响,分析相应的影响因素对提高受胎率具有重要价值。 展开更多
关键词 胚胎因素 肉羊 胚胎移植 效果 影响
下载PDF
反复种植失败的胚胎学因素研究进展
5
作者 王晓杰 杨晓葵 李颖 《中国医刊》 CAS 2023年第12期1293-1297,共5页
随着辅助生殖技术的不断发展和进步,越来越多的不孕夫妇从中获益。但是胚胎的反复种植失败也给患者带来了较大的心理和经济压力。由于胚胎反复种植失败的病因复杂,目前临床上仍缺乏有效的干预措施。胚胎因素也是影响植入成功的重要一环... 随着辅助生殖技术的不断发展和进步,越来越多的不孕夫妇从中获益。但是胚胎的反复种植失败也给患者带来了较大的心理和经济压力。由于胚胎反复种植失败的病因复杂,目前临床上仍缺乏有效的干预措施。胚胎因素也是影响植入成功的重要一环,基于此,本文就近年来反复种植失败的胚胎因素研究进展进行总结,以期为基础研究和临床治疗提供参考。 展开更多
关键词 体外受精胚胎移植术 反复种植失败 胚胎因素
下载PDF
影响牛胚胎移植妊娠率的因素分析 被引量:16
6
作者 和协超 季维智 《动物科学与动物医学》 2001年第4期11-14,共4页
关键词 胚胎移植 移植妊娠率 影响因素 胚胎因素 受体牛因素 技术因素
下载PDF
影响奶牛体外受精胚胎培养效果的胚胎因素 被引量:3
7
作者 佟桂芝 董正德 《当代畜牧》 2007年第1期33-35,共3页
对胚胎因素影响奶牛体外受精胚胎培养效果进行了研究。结果发现,在胚胎因素中,胚胎类型(冻胚和鲜胚)与胚胎发育阶段对胚胎培养效果没有显著影响(P>0.05);冷冻胚胎解冻后停留时间对胚胎培养效果影响极显著(P<0.01);冷冻胚胎解冻后... 对胚胎因素影响奶牛体外受精胚胎培养效果进行了研究。结果发现,在胚胎因素中,胚胎类型(冻胚和鲜胚)与胚胎发育阶段对胚胎培养效果没有显著影响(P>0.05);冷冻胚胎解冻后停留时间对胚胎培养效果影响极显著(P<0.01);冷冻胚胎解冻后胚胎等级对胚胎培养效果影响显著(P<0.05),解冻后A级胚胎存活率(80%,128/160)、囊胚孵化率(58.75%,94/160)显著高于B级胚胎存活率(59.38%,95/160)和囊胚孵化率(37.5%,60/160)(P<0.05);解冻水浴温度(20℃、30℃)对培养效果有极显著影响(P<0.01)。 展开更多
关键词 奶牛 体外受精胚胎 胚胎因素 胚胎存活率 囊胚孵化率
原文传递
影响羊胚胎移植妊娠率的因素 被引量:2
8
作者 宋振清 《中国畜牧兽医文摘》 2013年第5期57-57,94,共2页
影响绵羊胚胎移植后能否继续妊娠的原因较多,而且十分复杂,包括生理、内分泌、遗传、免疫和环境等诸多因素。目前,许多专家和学者把这些因素归纳为3个方面,即胚胎因素、受体因素和其他因素。根据笔者多年的实践观察和调查总结认为,环境... 影响绵羊胚胎移植后能否继续妊娠的原因较多,而且十分复杂,包括生理、内分泌、遗传、免疫和环境等诸多因素。目前,许多专家和学者把这些因素归纳为3个方面,即胚胎因素、受体因素和其他因素。根据笔者多年的实践观察和调查总结认为,环境是影响胚胎移植妊娠率的重要因素之一。家畜环境卫生学中指出,外界环境是指大气、水域、土壤等三要素所构成的自然整体,是一切生物赖以生存的物质基础。 展开更多
关键词 移植妊娠率 胚胎移植 绵羊 家畜环境卫生学 胚胎因素 外界环境 物质基础 内分泌
下载PDF
影响肉绵羊胚胎移植的因素
9
作者 隋国香 《现代畜牧科技》 2015年第12期47-47,共1页
1胚胎因素胚胎采集。采胚方法可选择输卵管采集法和子宫采集法。输卵管采集法一般是在供体母羊受精后前4天使用,尽管具有很高的回收率,但术后非常容易导致卵巢和输卵管伞出现黏连,从而使卵子以后的接收和运行过程受到影响,如果出现... 1胚胎因素胚胎采集。采胚方法可选择输卵管采集法和子宫采集法。输卵管采集法一般是在供体母羊受精后前4天使用,尽管具有很高的回收率,但术后非常容易导致卵巢和输卵管伞出现黏连,从而使卵子以后的接收和运行过程受到影响,如果出现双侧同时黏连的现象,甚至可能使其终生无法受孕。目前, 展开更多
关键词 胚胎移植 肉绵羊 采集法 输卵管 胚胎采集 胚胎因素 供体母羊 运行过程
下载PDF
Identification of two distinct transactivation domains in the pluripotency sustaining factor nanog 被引量:22
10
作者 GUANGJinPAN DUANQINGPEI 《Cell Research》 SCIE CAS CSCD 2003年第6期499-502,共4页
Nanog is a newly identified homeodomain gene that functions to sustain the pluripotency of embryonic stem cells.However,the molecular mechanism through which nanog regulates stem cell pluripotency remains unknown.Mous... Nanog is a newly identified homeodomain gene that functions to sustain the pluripotency of embryonic stem cells.However,the molecular mechanism through which nanog regulates stem cell pluripotency remains unknown.Mouse nanog encodes a polypeptide of 305 residues with a divergent homeodomain similar to those in the NK-2 family.The rest ofnanog contains no apparent homology to any known proteins characterized so far.It is hypothesized that nanog encodes a transcription factor that regulates stem cell pluripotency by switching on or off target genes.To test this hypothesis,we constructed fusion proteins between nanog and DNA binding domains of the yeast transcription factor Gal4 and tested the transactivation potentials of these constructs.Our data demonstrate that both regions N- and C- terminal to the homeodomain have transcription activities.Despite the fact that it contains no apparent transactivation motifs,the C-terminal domain is about 7 times as active as the N-terminal one.This unique arrangement of dual transactivators may confer nanog the flexibility and specificity to regulate downstream genes critical for both pluripotency and differentiation of stem cells. 展开更多
关键词 NANOG PLURIPOTENCY stem cells self renewal transactivation domain HOMEOPROTEIN HOMEODOMAIN
下载PDF
Differentiation of embryonic stem cells into insulin-producing cells promoted by Nkx2.2 gene transfer 被引量:9
11
作者 Akira Shiroi Shigehiko Ueda +6 位作者 Yukiteru Ouji Ko Saito Kei Moriya Yuko Sugie Hiroshi Fukui Shigeaki Ishizaka Masahide Yoshikawa 《World Journal of Gastroenterology》 SCIE CAS CSCD 2005年第27期4161-4166,共6页
AIM: To investigate the ability of a genetically altered embryonic stem (ES) cell line to generate insulin-producing cells in vitro following transfer of the Nkx2.2 gene.METHODS: Hamster Nkx2.2 genes were transfer... AIM: To investigate the ability of a genetically altered embryonic stem (ES) cell line to generate insulin-producing cells in vitro following transfer of the Nkx2.2 gene.METHODS: Hamster Nkx2.2 genes were transferred into mouse ES cells. Parental and Nkx2.2-transfected ES cells were initiated toward differentiation in embryoid body (EB) culture for 5 d and the resulting EBs were transferred to an attached culture system. Dithizone (DTZ), a zincchelating agent known to selectively stain pancreatic beta cells, was used to detect insulin-producing cells.The outgrowths were incubated in DTZ solution (final concentration, 100μg/mL) for 15 rain before being examined microscopically. Gene expression of the endocrine pancreatic markers was also analyzed by RT-PCR. In addition, insulin production was determined immunohistochemically and its secretion was examined using an ELISA.RESULTS: DTZ-stained cellular clusters appeared after approximately 14 d in the culture of Nkx2.2-transfected ES cells (Nkx-ES cells), which was as much as 2 wk earlier, than those in the culture of parental ES cells (wt-ES). The frequency of DTZ-positive cells among total cultured cells on day 28 accounted for approximately 1.0% and 0.1% of the Nkx-ES- and wt-ES-derived EB outgrowths, respectively. The DTZ-positive cellular clusters were found to be immunoreactive to insulin, while the gene expressions of pancreatic-duodenal homeobox 1 (PDX1), proinsulin 1 and proinsulin 2 were observed in the cultures that contained DTZ-positive cellular clusters.Insulin secretion was also confirmed by ELISA, whereas glucose-dependent secretion was not demonstrated.CONCLUSION: Nkx2.2-transfected ES cells showed an ability to differentiate into insulin-producing cells. 展开更多
关键词 ANIMALS Cell Differentiation Cell Line CRICETINAE Gene Transfer Techniques Homeodomain Proteins Insulin Islets of Langerhans MICE Mice Inbred Strains Stem Cells Transcription Factors
下载PDF
Development of a Synthetic Medium for the in Vitro Culture of Bovine Embryos
12
作者 Moreno D. Neira A. +5 位作者 Dubreil L. Liegeois L. Destrumelle S. Briand-Amirat L. Bencharif D. Tainturier D. 《Journal of Agricultural Science and Technology(A)》 2014年第8期679-685,共7页
The aim of this study was to develop a synthetic medium for the in vitro culture of bovine embryos, using various growth factors and cytokines (GF-CYK): insulin-like growth factorl (IGF-Ⅰ), insulin-like growth f... The aim of this study was to develop a synthetic medium for the in vitro culture of bovine embryos, using various growth factors and cytokines (GF-CYK): insulin-like growth factorl (IGF-Ⅰ), insulin-like growth factorⅡ (IGF-Ⅱ), basic fibroblast growth factor (bFGF), leukemia inhibitory factor (LIF), granulocyte-macrophage colony stimulating factor (GM-CSF) and transforming growth factor beta Ⅰ (TGF-β1) + hyaluronan (HA) + recombinant albumin (RA). The embryos were cultured in synthetic oviduct fluid (SOF) supplemented with: treatment 1 (T1): bovine serum albumin (BSA) + insulin, transferrin and selenium (ITS) (control); or treatment 2 (T2): GF-CYK + HA + RA. The blastocyst rates were not significantly different between TI and T2, at seven days post fertilization (dpf) (28.9% ± 2.4% and 31.8% ±2.2%), and at 8 dpf (36.5% ±2.4% and 39.1% ±1.9%), respectively (P 〉 0.05). The total cell number (TCN) was significantly higher with T2 than that with T1 at 7 dpf(164.9 ±5.3 and 149.7 ±4.0) and 8 dpf (182.7 ±6.4 and 165.0 ±5.5) (P 〈 0.05). The blastocyst diameter obtained with T2 was significantly greater (P 〈 0.05) than with T1 at 7 dpf (173.3 μm ±4.9 μm and 157.2μm ±4.1 μm, respectively), however, no significant differences were observed at 8 dpf (190.3 μm 5.2 μm and 179.7 μm ± 5.3 μm, respectively). In conclusion, the synthetic medium (T2) shows a comparable development rate to the control medium and improves the blastocyst diameter and the TCN. 展开更多
关键词 Growth factors CYTOKINES synthetic media.
下载PDF
辅助生殖技术后异位妊娠的防治 被引量:7
13
作者 黄薇 朱慧莉 《山东大学学报(医学版)》 CAS 北大核心 2019年第10期27-32,共6页
辅助生殖技术(ART)已成为导致异位妊娠的主要因素之一,但其确切机制尚不清楚。目前研究认为输卵管因素、超生理的激素环境对子宫内膜容受性的影响、胚胎发育潜能、ART技术操作等可能是ART后异位妊娠的高危因素。从输卵管因素、胚胎因素... 辅助生殖技术(ART)已成为导致异位妊娠的主要因素之一,但其确切机制尚不清楚。目前研究认为输卵管因素、超生理的激素环境对子宫内膜容受性的影响、胚胎发育潜能、ART技术操作等可能是ART后异位妊娠的高危因素。从输卵管因素、胚胎因素、辅助生殖技术操作及特殊类型的异位妊娠等方面,讨论ART后异位妊娠的防治。 展开更多
关键词 辅助生殖技术 异位妊娠 输卵管因素 胚胎因素
原文传递
Growth of embryo and gene expression of nutrient transporters in the small intestine of the domestic pigeon(Columba livia) 被引量:1
14
作者 Ming-xia CHEN Xiang-guang LI +4 位作者 Jun-xian YANG Chun-qi GAO Bin WANG Xiu-qi WANG Hui-chao YAN 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2015年第6期511-523,共13页
The objective of this study was to investigate the relationship between gene expression of nutrient(amino acid, peptide, sodium and proton) transporters in the small intestine and embryonic growth in domestic pigeon... The objective of this study was to investigate the relationship between gene expression of nutrient(amino acid, peptide, sodium and proton) transporters in the small intestine and embryonic growth in domestic pigeons(Columba livia). One hundred and twenty-five fertilized eggs were randomly assigned into five groups and were incubated under optimal conditions(temperature of 38.1 °C and relative humidity of 55%). Twenty embryos/birds from each group were sacrificed by cervical dislocation on embryonic day(E) 9, 11, 13, 15 and day of hatch(DOH). The eggs, embryos(without yolk sac), and organs(head, brain, heart, liver, lungs, kidney, gizzard, small intestine, legs, and thorax) were dissected, cleaned, and weighed. Small intestine samples were collected for RNA isolation. The m RNA abundance of intestinal nutrient transporters was evaluated by real-time reverse transcription-polymerase chain reaction(RT-PCR). We classified these ten organs into four types according to the changes in relative weight during embryonic development. In addition, the gene expression of nutrient transporters was differentially regulated by embryonic day. The m RNA abundances of b^0,+AT, EAAT3, y^+LAT2, Pep T1, LAT4, NHE2, and NHE3 increased linearly with age, whereas m RNA abundances of CAT1, CAT2, LAT1, EAAT2, SNAT1, and SNAT2 were increased to higher levels on E9 or E11 and then decreased to lower levels until DOH. The results of correlation analysis showed that the gene expressions of b^0,+AT, EAAT3, Pep T1, LAT4, NHE2, NHE3, and y^+LAT2 had positive correlations with body weight(0.71〈correlation coefficient(CC)〈0.82, P〈0.0001), while CAT1, CAT2, EAAT2, SNAT1, and SNAT2 had negative correlations with body weight(-0.86〈CC〈-0.64, P〈0.0001). The gene expressions of b^0,+AT, EAAT3, LAT4, Pep T1, NHE2, NHE3, and y^+LAT2 showed positive correlations with intestinal weight(0.80〈CC〈0.91, P〈0.0001), while CAT1, CAT2, and EAAT2 showed negative correlations with intestinal weight(-0.84〈CC〈-0.67, P〈0.0001). It was concluded that the differences between growth trajectories of organs and gene expression of nutrient transporters in small intestine were due to their functional and physiological properties, which provided a comprehensive study of amino acid and peptide transporter m RNA in the small intestine during embryonic growth of pigeons. 展开更多
关键词 Embryonic growth Nutrient transporters Gene expression Small intestine PIGEONS
原文传递
Effect of soothing liver therapy on oocyte quality and growth differentiation factor-9 in patients undergoing in vitro fertilization and embryo transfer 被引量:11
15
作者 Xing Gao Xiufeng Chang +3 位作者 Huilan Du Min Zhang Jianping Zhang Aiping Zhu 《Journal of Traditional Chinese Medicine》 SCIE CAS CSCD 2013年第5期597-602,共6页
OBJECTIVE:To investigate the effect of Soothing liver therapy on infertile women undergoing in vitro fertilization and embryo transfer(IVF-ET)and to explore its mechanism.METHODS:Fifty-eight women with tubal infertili... OBJECTIVE:To investigate the effect of Soothing liver therapy on infertile women undergoing in vitro fertilization and embryo transfer(IVF-ET)and to explore its mechanism.METHODS:Fifty-eight women with tubal infertility were randomized into two groups:30 in an experimental group treated with Xiaoyao powder(Shugan)plus gonadotropin-releasing hormone analog(GnRHa)/follicle-stimulating hormone(FSH)/human chorionic gonadotropin(hCG)and 28 in the control group who were treated with GnRHa/FSH/hCG only.The total gonadotropin(Gn)doses required,endometrial thickness,oocyte numbers,high quality embryo production rate and pregnancy rate of the two groups were compared.The concentration of growth differentiation factor-9(GDF-9)in follicular fluid was detected by western blotting and the expression of GDF-9 mRNA in granulosa cells was measured using reverse tran-scription-polymerase chain reaction amplification.RESULTS:In the experimental group,the Gn dose was significantly lower than that in the control group;the endometrial thickness,high quality embryo production and pregnancy rates were significantly higher and the expression of GDF-9 mRNA was also significantly higher than in the control group(all P<0.05).CONCLUSION:Shugan treatment can improve the pregnancy rate of women with tubal infertility;its mechanism is possibly related to the increased expression of GDF-9 in granulosa cells. 展开更多
关键词 Fertilization in vitro Embryo transfer Growth differentiation factor 9 Follicular fluid Soothing liver therapy
原文传递
上一页 1 下一页 到第
使用帮助 返回顶部