The effect of different somatic cell cultures on mouse embryo development at early cleavage stage in vitro was studied.Oviductal epithelial,cumulus,uterine of fibroblast cells were co-cultured with mouse embryos respe...The effect of different somatic cell cultures on mouse embryo development at early cleavage stage in vitro was studied.Oviductal epithelial,cumulus,uterine of fibroblast cells were co-cultured with mouse embryos respectively for 4 days,Embryos at 1-cell stage were collected from swollen ampullae of the oviducts.A total number of 121,111,132 and 89 embyos were co-cultured with oviduct epithelial,cumulus,uterine and fibroblast cells respectively,Among them,109,88,101 and 20 embryos developed regularly to the blastocyst stage respectively.The blastocysts were transferred to the uteri of synchronized recipient mice,only 9 blastocysts co-cultured with oviductal epithelial cells and 6 blastocysts with cumulus cells had developed normally and been implanted.These data indicated that oviductal epithelial cells and cumulus cells exert a specific promoting action on early embryonic development in vitro.展开更多
文摘The effect of different somatic cell cultures on mouse embryo development at early cleavage stage in vitro was studied.Oviductal epithelial,cumulus,uterine of fibroblast cells were co-cultured with mouse embryos respectively for 4 days,Embryos at 1-cell stage were collected from swollen ampullae of the oviducts.A total number of 121,111,132 and 89 embyos were co-cultured with oviduct epithelial,cumulus,uterine and fibroblast cells respectively,Among them,109,88,101 and 20 embryos developed regularly to the blastocyst stage respectively.The blastocysts were transferred to the uteri of synchronized recipient mice,only 9 blastocysts co-cultured with oviductal epithelial cells and 6 blastocysts with cumulus cells had developed normally and been implanted.These data indicated that oviductal epithelial cells and cumulus cells exert a specific promoting action on early embryonic development in vitro.
