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体外授精胚胎移植治疗受孕障碍81周期分析
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作者 冯玉华 汤莉莎 +3 位作者 张若鹏 李大兵 马雪花 吴风 《淮海医药》 2001年第6期470-471,共2页
目的 分析体外授精胚胎移植 (IVF- ET)及卵细胞浆内单精子注射 (ICSI)治疗受孕障碍的技术研究和治疗结果。方法 采用超排卵方案诱导卵泡生长发育 ,经阴道穿刺取卵在体外授精 ,对严重少弱精者采用 ICSI技术 ,受精卵在体外培养 2~ 3天... 目的 分析体外授精胚胎移植 (IVF- ET)及卵细胞浆内单精子注射 (ICSI)治疗受孕障碍的技术研究和治疗结果。方法 采用超排卵方案诱导卵泡生长发育 ,经阴道穿刺取卵在体外授精 ,对严重少弱精者采用 ICSI技术 ,受精卵在体外培养 2~ 3天后移植入子宫内发育。结果 治疗 81周期中移植周期 72个 ,其中 41例妊娠 ,妊娠率5 6 .9% (41/72 ) ,明显高于国内目前报道。结论 IVF- ET是治疗输卵管阻塞等女性不孕的有效方法 ,ICSI在男性不育的治疗中有肯定疗效 ,提高妊娠率的关键在于先进的促排卵技术 ,层流净化胚胎实验室及良好的胚胎操作技术 。 展开更多
关键词 女(雌)性不育 体外受精 胚胞移植
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试管婴儿孕妇的产科处理(附20例分析)
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作者 李小毛 庄广伦 +3 位作者 李大慈 周灿权 李洁 李俐琳 《中山大学学报(医学科学版)》 CAS CSCD 1995年第S1期98-98,共1页
试管婴儿孕妇的产科处理(附20例分析)李小毛,庄广伦,李大慈,周灿权,李洁,李俐琳(中山医科大学附属第一医院妇产科;广州。510080)主题词受精,体外;胚胞移植;围产医学中图号R321.33我院1990年建立体外授... 试管婴儿孕妇的产科处理(附20例分析)李小毛,庄广伦,李大慈,周灿权,李洁,李俐琳(中山医科大学附属第一医院妇产科;广州。510080)主题词受精,体外;胚胞移植;围产医学中图号R321.33我院1990年建立体外授精与胚胎移植实验室,1991年初第... 展开更多
关键词 受精 体外 胚胞移植 围产医学
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Development in vitro in Reconstitution of Embryo from Porcine Somatic Cell Nuclear Transfer 被引量:3
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作者 张德福 王英 +3 位作者 陈茵 王凯 Karl Schellander LIN Cai-lu 《Developmental and Reproductive Biology》 2002年第1期13-22,共10页
In present paper,a study on reconstitution of porcine oocytes by using nuclear transfer with cumulus cells(CC) and fibroblast cells(FC) was carried out.Reconstituted oocytes which were the fusion with CC and showed a ... In present paper,a study on reconstitution of porcine oocytes by using nuclear transfer with cumulus cells(CC) and fibroblast cells(FC) was carried out.Reconstituted oocytes which were the fusion with CC and showed a cleavage rate of 56.7%,developed into morula (11.7%) and blastocysts (6.7%) phases which were higher than those derived from the fusion with FC( P <0.05).The results of this study also involved the effects of oocyte collection method,activation protocol and maturational age of recipient oocytes during the in vitro develpoment of nuclear transfer embryos which were reconstituted with cultured cumulus cells.The cumulus cells synchronized in G 0/G 1 phases through serum starvation culture,were transferred into enuclated oocytes which were collected by aspiration or dissection method and cultured for 33 or 44 h.Reconstituted embryos were activated with a combination of calcium ionophore A23187 or electric pulsation and 6 DMAP,and cultured for 6 days.As for the oocyte collection methods,activation treatment in the presence of cytochalasin B and activation protocols did not affect the developmental rate of embryos reconstituted with 44 h mature recipients.However,the development rate of reconstituted embryos with 33 h mature recipients were significantly higher( P <0.05) by activation with the combination of electric pulsation and 6 DMAP.These results suggest that the reconstituted porcine embryos derived from cultured cumulus cells can develop into the blastocyst stage and that the development of the former could be improved for the reconstitution with young oocyte cytoplast after the activation with the combination of electric pulsation and 6 DMAP. 展开更多
关键词 PIG nuclear transfer (NT) activation method oocyte age
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“杂交魔术”登上畜牧产业大舞台
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作者 杨俊杰 高新让 《中国科技信息》 1997年第1期18-19,共2页
许多科学家预言,21世纪是生物技术的鼎盛时代,生物技术仍是自然科学领域中的带头学科,生物技术冲刺21世纪,将来畜牧业问题的出路最终要由生物技术来解决,也将成为下世纪发展最迅速的高新技术产业——“朝阳产业”。到2000年,人类食物的... 许多科学家预言,21世纪是生物技术的鼎盛时代,生物技术仍是自然科学领域中的带头学科,生物技术冲刺21世纪,将来畜牧业问题的出路最终要由生物技术来解决,也将成为下世纪发展最迅速的高新技术产业——“朝阳产业”。到2000年,人类食物的1/5可利用生物技术生产以满足人们生存和发展的需求。 国内外最新科技信息表明,现代生物技术的研究应用已经从医药、食品工业扩展到农牧业,因此90年代末和21世纪,畜牧业的发展和生物技术在畜牧上的研究应用开发,前景更为广阔,成为畜牧业发展的主角。 展开更多
关键词 畜牧产业 畜禽良种 繁殖 动物胚胞移植 杂交
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Embryonic stem cells generated by nuclear transfer of human somatic nuclei into rabbit oocytes 被引量:57
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作者 YINGCHEN ZHIXuHE +19 位作者 AILIANLIU KAIWANG WENWEIMAO JIANKINCHU YONGLU ZHENGFUFANG YINGTANGSHI QINGZHANGYANG DAYUANCHEN MINKANGWANG JINSONGLI SHAOLIANGHUANG XIANGYINKONG YAOZHOUSHI ZHIQIANGWANG JIAHuIXIA ZHIGAOLONG ZHIGANGXUE WENXIANGDING HUIZHENSHENG 《Cell Research》 SCIE CAS CSCD 2003年第4期251-263,共13页
To solve the problem of immune incompatibility, nuclear transplantation has been envisaged as a means to produce cells or tissues for human autologous transplantation. Here we have derived embryonic stem cells by the ... To solve the problem of immune incompatibility, nuclear transplantation has been envisaged as a means to produce cells or tissues for human autologous transplantation. Here we have derived embryonic stem cells by the transfer of human somatic nuclei into rabbit oocytes. The number of blastocysts that developed from the fused nuclear transfer was comparable among nuclear donors at ages of 5, 42, 52 and 60 years, and nuclear transfer (NT) embryonic stem cells (ntES cells) were subsequently derived from each of the four age groups. These results suggest that human somatic nuclei can form ntES cells independent of the age of the donor. The derived ntES cells are human based on karyotype, isogenicity, in situ hybridization, PCR and immunocytochemistry with probes that distinguish between the various species. The ntES cells maintain the capability of sustained growth in an undifferentiated state, and form embryoid bodies, which, on further induction, give rise to cell types such as neuron and muscle, as well as mixed cell populations that express markers representative of all three germ layers. Thus, ntES cells derived from human somatic cells by NT to rabbit eggs retain phenotypes similar to those of conventional human ES cells, including the ability to undergo multilineage cellular differentiation. 展开更多
关键词 nuclear transfer (NT) somatic cell nuclear transfer (SCNT) embryonic stem cells (ES cell) therapeutic cloning rabbit oocyte.
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Diminution of toxic copper accumulation in toxic milk mice modeling Wilson disease by embryonic hepatocyte intrasplenic transplantation 被引量:9
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作者 ZhuShi Xiu-LingLiang +5 位作者 Bing-XunLu Su-YuePan XiChen Qi-QiangTang YingWang FanHuang 《World Journal of Gastroenterology》 SCIE CAS CSCD 2005年第24期3691-3695,共5页
AIM: To observe the therapeutic effect of intrasplenic transplantation with embryonic hepatocytes on amelioration of hereditary copper accumulation in toxic milk (TX) mouse modeling Wilson disease. METHODS: Donor hepa... AIM: To observe the therapeutic effect of intrasplenic transplantation with embryonic hepatocytes on amelioration of hereditary copper accumulation in toxic milk (TX) mouse modeling Wilson disease. METHODS: Donor hepatocytes were harvested from 14-d fetal liver of a pregnant homogeneous DL mouse. These cells were successively cultured, labeled with fluorescein dye Hoechst 33342 for 24 h, and sequentially infused into the spleen parenchyma of the recipient TX mice. No host immunosuppression measures were taken. Two and four weeks after transplantation, the recipients were killed for routine histologic investigation and immunohistochemistry study up to 4 wk after transplantation. The serum copper and ceruloplasmin concentrations of the recipient mice were determined by graphite furnace atomic absorption spectroscopy.RESULTS: In the following 2nd and 4th wk after transplantation, the donor hepatocytes could be visualized in the livers of 47.3% recipients. The serum ceruloplasmin and copper concentrations increased by 1.6-fold after 2 wk and 2.0-fold times after 4 wk respectively, which ultimately rose from about 30% of the normal level to nearly 60%(P<0.01). The hepatic copper concentration decreased 7.2%, 4 wk after transplantation. Pathologic examination showed that there were many actively proliferative hepatocyte precursor cells with specific embryonic hepatocyte marker AFP migrated into hepatic sinusoidsof the recipients. A large number of cells carrying hepatocytes marker and albumin were observed in the recipient spleen tissues.CONCLUSION: Embryonic hepatocytes are capable of differentiating into mature hepatocytes in vivo. After transplantation, the hereditary abnormalities of copper metabolism in TX mice could be corrected partially by intrasplenic transplantation of homogeneous embryonic hepatocytes. 展开更多
关键词 Transplantation Wilson disease COPPER CERULOPLASMIN
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Establishment of customized mouse stem cell lines by sequential nuclear transfer 被引量:1
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作者 Chunli Zhao Ruqiang Yao +14 位作者 Jie Hao Chenhui Ding Yong Fan Xiangpeng Dai Wei Li Tang Hai Zichuan Liu Yang Yu Yingying Wang Xiaojun Hou Weizhi Ji Qi Zhou Alice Jouneau Fanyi Zeng Liu Wang 《Cell Research》 SCIE CAS CSCD 2007年第1期80-87,共8页
Therapeutic cloning, whereby embryonic stem cells (ESCs) are derived from nuclear transfer (NT) embryos, may play a major role in the new era of regenerative medicine. In this study we established forty nuclear tr... Therapeutic cloning, whereby embryonic stem cells (ESCs) are derived from nuclear transfer (NT) embryos, may play a major role in the new era of regenerative medicine. In this study we established forty nuclear transfer-ESC (NTESC) lines that were derived from NT embryos of different donor cell types or passages. We found that NT-ESCs were capable of forming embryoid bodies. In addition, NT-ESCs expressed pluripotency stem cell markers in vitro and could differentiate into embryonic tissues in vivo. NT embryos from early passage RI donor cells were able to form full term developed pups, whereas those from late passage RI ES donor cells lost the potential for reprogramming that is essential for live birth. We subsequently established sequential NT-RI-ESC lines that were developed from NT blastocyst of late passage R 1 ESC donors. However, these NT-R I-ESC lines, when used as nuclear transfer donors at their early passages, failed to result in live pups. This indicates that the therapeutic cloning process using sequential NT-ESCs may not rescue the developmental deficiencies that resided in previous donor generations. 展开更多
关键词 nuclear transfer therapeutic cloning embryonic stem cells
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Embryonic stem cells develop into hepatocytes after intrasplenic transplantation in CCl_4-treated mice 被引量:5
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作者 Kei Moriya Masahide Yoshikawa +5 位作者 Ko Saito Yukiteru Ouji Mariko Nishiofuku Noriko Hayashi Shigeaki Ishizaka Hiroshi Fukui 《World Journal of Gastroenterology》 SCIE CAS CSCD 2007年第6期866-873,共8页
AIM: To transplant undifferentiated embryonic stem (ES) cells into the spleens of carbon tetrachloride (CCl4)-treated mice to determine their ability to differentiate into hepatocytes in the liver. METHODS: CCh,... AIM: To transplant undifferentiated embryonic stem (ES) cells into the spleens of carbon tetrachloride (CCl4)-treated mice to determine their ability to differentiate into hepatocytes in the liver. METHODS: CCh, 0.5 mL/kg body weight, was injected into the peritoneum of C57BL/6 mice twice a week for 5 wk. In group 1 (n = 12), 1 × 10^5 undifferentiated ES cells (0.1 mL of 1 × 10^6/mL solution), genetically labeled with GFP, were transplanted into the spleens 1 d after the second injection. Group 2 mice (n = 12) were injected with 0.2 mL of saline twice a week, instead of CCh, and the same amount of ES cells was transplanted into the spleens. Group 3 mice (n = 6) were treated with CCh and injected with 0.1 mL of saline into the spleen, instead of ES cells. Histochemical analyses of the livers were performed on post-transplantation d (PD) 10, 20, and 30. RESULTS: Considerable numbers of GFP-immunopositive cells were found in the periportal regions in group 1 mice (CCh-treated) on PD 10, however, not in those untreated with CCh (group 2). The GFP-positive cells were also immunopositive for albumin (ALB), alpha-1 antitrypsin, cytokeratin 18, and hepatocyte nuclear factor 4 alpha on PD 20. Interestingly, most of the GFP-positive cells were immunopositive for DLK, a hepatoblast marker, on PD 10. Although very few ES-derived cells were demonstrated immunohistologically in the livers of group 1 mice on PD 30, improvements in liver fibrosis were observed. Unexpectedly, liver tumor formation was not observed in any of the mice that received ES cell transplantation during the experimental period CONCLUSION: Undifferentiated ES cells developed into hepatocyte-like cells with appropriate integration into tissue, without uncontrolled cell growth. 展开更多
关键词 Embryonic stem cells Hepatic differentiation Intrasplenic transplantation Carbon tetrachloride
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Future of liver transplantation: Non-human primates for patient-specific organs from induced pluripotent stem cells 被引量:2
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作者 Madhusudana Girija Sanal 《World Journal of Gastroenterology》 SCIE CAS CSCD 2011年第32期3684-3690,共7页
Strategies to fill the huge gap in supply versus demand of human organs include bioartificial organs, growing humanized organs in animals, cell therapy, and implantable bioengineered constructs. Reproducing the comple... Strategies to fill the huge gap in supply versus demand of human organs include bioartificial organs, growing humanized organs in animals, cell therapy, and implantable bioengineered constructs. Reproducing the complex relations between different cell types, generation of adequate vasculature, and immunological complications are road blocks in generation of bioengineered organs, while immunological complications limit the use of humanized organs produced in animals. Recent developments in induced pluripotent stem cell (iPSC) biology offer a possibility of generating human, patient-specific organs in non-human primates (NHP) using patient-derived iPSC and NHP-derived iPSC lacking the critical developmental genes for the organ of interest complementing a NHP tetraploid embryo. The organ derived in this way will have the same human leukocyte antigen (HLA) profile as the patient. This approach can be curative in genetic disorders as this offers the possibility of gene manipulation and correction of the patient's genome at the iPSC stage before tetraploid complementation. The process of generation of patient-specific organs such as the liver in this way has the great advantage of making use of the natural signaling cascades in the natural milieu probably resulting in organs of great quality for transplantation. However, the inexorable scientific developments in this direction involve several social issues and hence we need to educate and prepare society in advance to accept the revolutionary consequences, good, bad and ugly. 展开更多
关键词 Induced pluripotent stem cells Hepatocytes TETRAPLOID Non-human primates ANENCEPHALY CHIMPANZEE Fumaryl acetoacetate hydrolase deficient Hhex
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Effect of electroacupuncture on oocyte quality and pregnancy of patients with PCOS undergoing in vitro fertilization and embryo transfer 被引量:6
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作者 崔薇 李静 +1 位作者 孙伟 闻姬 《World Journal of Acupuncture-Moxibustion》 2012年第1期23-29,共7页
Objective To observe the impacts of electroacupuncture (EA) on oocyte quality and pregnant outcome for the patients with polycystic ovary syndrome (PCOS) undergoing in vitro fertilization and embryo transfer (IVF... Objective To observe the impacts of electroacupuncture (EA) on oocyte quality and pregnant outcome for the patients with polycystic ovary syndrome (PCOS) undergoing in vitro fertilization and embryo transfer (IVF-ET) and explore its potential mechanism. Methods Sixty-six patients with PCOS and undergoing IVF-ET were divided into two groups randomly, including an observation group (34 cases) and a control group (32 cases). Ethinylestradiol and cyproterone acetate tablets and gonadotropin-releasing hormone agonist were administered for long-program superovulation in either group. In the observation group, the intervention of EA was applied to Guānyuán (关元 CV 4), Zhōngjí (中极 CV 3), Sānyīnjiāo (三阴交 SP 6), Zǐgōng (子宫 EX-CA 1) and Tàixī (太溪 KI 3) additionally for 30 min, once daily, 1 menstrual cycle before controlled ovarian hyperstimulation (COH) and during COH. The pregnant outcome, evaluation of kidney deficiency syndrome, blood hormone level on the day of human chorionic gonadotropin injection and the concentrations of stem cell factor (SCF) in the serum and follicular fluid on the day of oocyte collection were compared between the two groups. Results The score of kidney deficiency symptoms was reduced remarkably after treatment in either group and the improvement in the observation group was superior to that in the control group (P0.01). The fertilization rate [(76.25?±?20.33)% vs (66.34?±?15.44)%], cleavage rate [(98.66?±?3.70)% vs (94.47?±?9.45)%] and the rate of high-quality embryos [(60.20?±?22.20)% vs (50.55?±?16.15)%] in the observation group were all superior to those in the control group separately (all P0.05). Clinical pregnancy rate (46.67%, 14/30) in the observation group was higher than that (37.93%, 11/29) in the control group, but without statistical difference (P0.05). SCF concentrations in the serum and follicular fluid on the day of oocyte collection in the observation group were higher obviously than those in the control group (both P0.05). Conclusion Electroacupuncture plays an active role in the pregnant outcomes of PCOS patients undergoing IVF-ET and it can relieve the symptoms of kidney deficiency in terms of TCM and improve clinical pregnant rate. The mechanism is relevant to the overall adjustment of organic endocrinal system and the local micro-environment of ovary and the improvement of oocyte quality through the up-regulation of SCF concentration. 展开更多
关键词 polycystic ovary syndrome ELECTROACUPUNCTURE in vitro fertilization and embryo transfer (IVF-ET) stem cell factor randomized controlled trial
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Identification of a suitable endogenous control gene in porcine blastocysts for use in quantitative PCR analysis of microRNAs 被引量:2
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作者 LI Jun WEI HengXi +2 位作者 LI Yan LI QiuYan LI Ning 《Science China(Life Sciences)》 SCIE CAS 2012年第2期126-131,共6页
To obtain reliable results in quantitative PCR (qPCR) reactions, an endogenous control (EC) gene is needed to correct for systematic variations. In this study, a TaqMan low density array was used to quantify the e... To obtain reliable results in quantitative PCR (qPCR) reactions, an endogenous control (EC) gene is needed to correct for systematic variations. In this study, a TaqMan low density array was used to quantify the expression levels of microRNA (rniRNA) genes in in vivo fertilized, in vitro fertilized, parthenogenetic and somatic cell nuclear transfer blastocysts. The aim was to identify suitable EC genes for the qPCR analysis of rniRNAs in porcine blastocysts. The results showed that thirty-six miRNAs were commonly expressed in the four kinds of embryos and the expression levels of eleven miRNAs were similar in the different embryo types (P-value〉0.05). These 11 miRNAs were selected as candidate EC genes for further analysis and, of these, miR-16 was identified as the most stable EC gene by the GeNorm (a tool based on a pair-wise comparison model that calculates the internal control genes stability measure and determines the most reliable pair of EC genes) and NorrnFinder (an excel plug-in that uses an ANOVA-based model to estimate intra- and inter- group variation to indicate the single most stable EC gene) programs. In addition, a cell number normalization method validated miR-16 as a suitable EC gene for use in future qPCR analysis of miRNAs in porcine blastocysts. 展开更多
关键词 endogenous control gene quantitative PCR microRNA BLASTOCYST
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Effects of acupuncture on ovarian blood supply and pregnancy outcomes in patients receiving assisted reproduction 被引量:1
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作者 Xu Zheng-zheng Gao Ying 《Journal of Acupuncture and Tuina Science》 CSCD 2018年第4期253-259,共7页
To observe the effects of acupuncture at the follicular phase on ovarian blood supply and pregnancy outcomes in patients who received in vitro fertilization/intracytoplasmic sperm injection-embry transfer (IVF/ICSI-E... To observe the effects of acupuncture at the follicular phase on ovarian blood supply and pregnancy outcomes in patients who received in vitro fertilization/intracytoplasmic sperm injection-embry transfer (IVF/ICSI-ET) of assisted reproductive technology (ART). Methods: A total of 169 IVF/ICSI-ET female recipients from the Reproductive Center of Xiehe Hospital, Tongji Medical College, Huazhong University of Science & Technology were randomized into an observation group (57 cases), a placebo group (54 cases) and a control group (58 cases). The observation group received acupuncture during the follicular phase, meanwhile the placebo group received placebo-acupuncture, and the control group did not receive acupuncture. The hemodynamic index, biochemical pregnancy rate and clinical pregnancy rate of each group were observed, respectively. Results: As to the ovarian arterial hemodynamic index, the pulsatility index (PI), resistance index (RI), and the systolic-to-diastolic peak velocity ratio (S/D) of the observation group were (0.819+0.131), (0.552+0.055) and (2.306+0.512), respectively, obviously lower than those in the placebo group and the control group, and the differences were statistically significant (all P〈0.05), but there were no statistically significant differences between the placebo group and the control group (all P〉0.05). As to the biochemical pregnancy rate and clinical pregnancy rate, the biochemical pregnancy rate in the observation group was 64.9% and the clinical pregnancy rate was 52.6%, which were significantly higher than those in the placebo group and the control group, and the differences were statistically significant (all P〈0.05), while there were no significant differences between the placebo group and the control group (both P〉0.05). Conclusion: Acupuncture treatment during the follicular phase can improve ovarian blood supply and pregnancy rate in those receiving IVF/ICSI-ET. 展开更多
关键词 Acupuncture Therapy INFERTILITY FEMALE Reproductive Technology Assisted Fertilization in Vitro SpermInjections INTRACYTOPLASMIC Embryo Transfer Pregnancy Rate
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Effect of soothing liver therapy on oocyte quality and growth differentiation factor-9 in patients undergoing in vitro fertilization and embryo transfer 被引量:11
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作者 Xing Gao Xiufeng Chang +3 位作者 Huilan Du Min Zhang Jianping Zhang Aiping Zhu 《Journal of Traditional Chinese Medicine》 SCIE CAS CSCD 2013年第5期597-602,共6页
OBJECTIVE:To investigate the effect of Soothing liver therapy on infertile women undergoing in vitro fertilization and embryo transfer(IVF-ET)and to explore its mechanism.METHODS:Fifty-eight women with tubal infertili... OBJECTIVE:To investigate the effect of Soothing liver therapy on infertile women undergoing in vitro fertilization and embryo transfer(IVF-ET)and to explore its mechanism.METHODS:Fifty-eight women with tubal infertility were randomized into two groups:30 in an experimental group treated with Xiaoyao powder(Shugan)plus gonadotropin-releasing hormone analog(GnRHa)/follicle-stimulating hormone(FSH)/human chorionic gonadotropin(hCG)and 28 in the control group who were treated with GnRHa/FSH/hCG only.The total gonadotropin(Gn)doses required,endometrial thickness,oocyte numbers,high quality embryo production rate and pregnancy rate of the two groups were compared.The concentration of growth differentiation factor-9(GDF-9)in follicular fluid was detected by western blotting and the expression of GDF-9 mRNA in granulosa cells was measured using reverse tran-scription-polymerase chain reaction amplification.RESULTS:In the experimental group,the Gn dose was significantly lower than that in the control group;the endometrial thickness,high quality embryo production and pregnancy rates were significantly higher and the expression of GDF-9 mRNA was also significantly higher than in the control group(all P<0.05).CONCLUSION:Shugan treatment can improve the pregnancy rate of women with tubal infertility;its mechanism is possibly related to the increased expression of GDF-9 in granulosa cells. 展开更多
关键词 Fertilization in vitro Embryo transfer Growth differentiation factor 9 Follicular fluid Soothing liver therapy
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