Tissue Culture and Rapid Multiplication Techniques of Apocynum L.

[Objective] This study aimed to investigate rapid multiplication of Apocynum by tissue culture so as to provide plantlet sources for its industrialized cultivation. [Method] The asepsis seedlings were obtained by dealing with Apocynum seeds. Its cotyledons, hypocotyls and shoot tips were cultured on the media containing different concentrations of hormones. Finally, the influence of different hormone combinations on differentiation of cotyledons and hypocotyls, rapid multiplication of shoot tips, rapid multiplication of regenerated shoots, and rooting of test-tube plantlets was com- pared. [Result] MS＋2.0 mg/L BA＋0.03 mg/L NAA and MS＋0.07 mg/L NAA were the optimum medium for inducing regenerated buds from cotyledons and hypocotyls re- spectively; MS＋2.0 mg/L BA＋0.02 mg/L NAA was the best medium for rapid multi- plication of shoot tips; MS＋1.9 mg/L BA＋I.7 mg/L NAA was the best medium for rapid multiplication of regenerated buds： and 1/2MS＋0.6 mg/L NAA was the best medium for inducing roots. [Conclusion] The optimum hormone combination was de- termined for Apocynum rapid multiplication by tissue culture, which provides technical support on Apocynum industrialized cultivation.

不同维生素浸种对玉米种子萌发的影响

以玉米杂交种郑单958为试验材料,采用不同质量浓度50、100、200 mg/L的水溶性维生素V_(B1)、V_(B2)、V_(B6)、V_(B12)、V_C、V_H、V_(PP)浸种24 h,处理后分析玉米种子萌发及胚芽和根生长中脯氨酸等可溶性调节物质情况。结果表明,供试维生素在100、200 mg/L处理下基本都呈现对种子萌发指数的促进,其中,100 mg/L维生素处理对种子萌发率促进效果显著;200 mg/L维生素处理对种子发芽势显著促进作用;50 mg/L维生素对上述3个指标的影响不同。与未处理对照相比,不同种类维生素和不同质量浓度的同一维生素对根冠比影响不同。维生素浸种均可引起玉米胚芽可溶性糖含量显著升高,外源维生素浸种处理可显著影响玉米种子萌发和胚芽及胚根的生长,且不同维生素在不同浓度下对各生长指标影响及脯氨酸等可溶性调节物质变化不尽相同,对种子萌发呈显著促进作用的V_(B2)和V_(B12)在玉米种子萌发中应用前景较好。