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创新思维 杏林增辉──记脑外科专家、留学回国人员胡卫星教授
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作者 刘小湄 《神州学人》 2003年第9期17-19,共3页
关键词 胡卫星 脑外科 美国阿拉巴马州立大学医学院 江苏省人民医院 跨国供职 南京医科大学 人才
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Development of Walnut EST-SSR Markers and Primer Design 被引量:3
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作者 冯怡 张智俊 +1 位作者 张舍龙 罗淑萍 《Agricultural Science & Technology》 CAS 2011年第12期1810-1813,共4页
[Objective] This research aimed to develop walnut EST-SSR markers and design corresponding primers.[Method] 5213 EST sequences of walnut(Juglans regia Linn.) public online in NCBI were used for character analysis wi... [Objective] This research aimed to develop walnut EST-SSR markers and design corresponding primers.[Method] 5213 EST sequences of walnut(Juglans regia Linn.) public online in NCBI were used for character analysis with bioinformatics methods,and primers were designed for the selected EST sequences by using Primer 3.0 software.[Result] 207 SSRs were obtained from the EST sequences,including 188 non-redundant sequences,the detection rate was 3.97% with an average distribution distance of 21.12 kb.Totally 92 types of repeat motifs were involved,which were mainly composed of dinucleotide and trinucleotide,accounting for 31.40% and 35.27% of the total number of repeat motifs,respectively.30 pairs of primers were initially selected from the 50 randomly-selected SSR primers by PCR amplification.[Conclusion] This research would lay foundations for the development of EST-SSR molecular markers in walnut and design of the targeted EST-SSR primers by mining and analyzing the SSR sites in walnut EST sequences. 展开更多
关键词 WALNUT EST-SSR MICROSATELLITE PRIMERS
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鸟语不再悦耳
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作者 胡卫星 《中学生语数外(高中版)》 2005年第4期6-7,共2页
不管从哪一个角度说,人,都比鸟聪明。人凭自己的聪明才智,将一只小鸟玩弄于股掌之上,应该不费吹灰之力。但有时却不是这样,比如当有些人利令智昏、欲壑难填、贪得无厌之时,就变得非常笨拙,谁都可以戏弄他们,比如《鸟与人》中所... 不管从哪一个角度说,人,都比鸟聪明。人凭自己的聪明才智,将一只小鸟玩弄于股掌之上,应该不费吹灰之力。但有时却不是这样,比如当有些人利令智昏、欲壑难填、贪得无厌之时,就变得非常笨拙,谁都可以戏弄他们,比如《鸟与人》中所写的故事就是如此。 展开更多
关键词 胡卫星 《鸟语不再悦耳》 散文 高中 语文 阅读欣赏
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Development of two microsatellite multiplex PCR systems for high throughput genotyping in Populus euphratica 被引量:4
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作者 Eusemann Pascal Fehrenz Steffen Schnittler Martin 《Journal of Forestry Research》 SCIE CAS CSCD 2009年第3期195-198,共4页
Eighteen microsatellite primer pairs previously developed at Oak Ridge National Laboratory for Populus tremuloides Michx. and Populus trichocarpa Tort. & Gray were screened for amplification in Euphrates poplar, Popu... Eighteen microsatellite primer pairs previously developed at Oak Ridge National Laboratory for Populus tremuloides Michx. and Populus trichocarpa Tort. & Gray were screened for amplification in Euphrates poplar, Populus euphratica Oliv. Thirteen loci were found to express polymorphisms ranging from two to 17 alleles. The eight most variable loci were selected to set up and optimize two multiplex polymerase chain reaction (PCR) assays. Three populations containing altogether 436 trees were used to characterize the selected loci and ascertain their applicability for parentage analysis and genotyping studies. Through cross-checking of clonal identity against sex of the genotyped trees we estimated the maximum error rate for merging genotypes to be less than 0.045. 展开更多
关键词 clone identification Euphrates poplar genetic fingerprint parentage analysis population structure SSR primers
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