Objective: To study the antifibrotic effects of genistein(GE) and quercetin(QU) on rat hepatic stellate HSC-T6 cell proliferation stimulated with platelet-derived growth factor (PDGF), collagen synthesis and type I pr...Objective: To study the antifibrotic effects of genistein(GE) and quercetin(QU) on rat hepatic stellate HSC-T6 cell proliferation stimulated with platelet-derived growth factor (PDGF), collagen synthesis and type I procollagen messenger RNA (mRNA) expression stimulated with transforming growth factor b1 (TGFb1). Methods: Cell proliferation was measured by crystal violet staining assay. Collagen synthesis was determined by 3H-proline incorporation assay. Type I procollagen mRNA level was determined by reverse transcription polymerase chain reaction (RT-PCR). Results: GE (25~70 mmolL-1) and QU (6.25~50 mmolL-1) concentration-dependently attenuated PDGF-driven HSC-T6 cell proliferative activity. TGFb1-stimulated collagen synthesis was also reduced. This was associated with a decrease in type I procollagen mRNA expression, indicating an effect at a pretranslational level. Conclusion: GE and QU may have therapeutic potential against liver fibrosis by regulating PDGF and TGFb1 actions.展开更多
Pretreatment of the corn stover powder by dilute sulphuric acid （solid-liquid ratio 1 ： 20） at 130 for 30 min was carried out with 89.09% of the hemicellulose removed. After filtration, the xylose-rich corn stover ...Pretreatment of the corn stover powder by dilute sulphuric acid （solid-liquid ratio 1 ： 20） at 130 for 30 min was carried out with 89.09% of the hemicellulose removed. After filtration, the xylose-rich corn stover pretreatment liquid, whose fermentable sugar was from hemicellulose hydrolysis only, consisting of 81.16% xylose and 15.27% glucose, was used to cultivate genetic recombinant Escherichia coli BL21 with human-like collagen （HLC） expression enhanced by 50.00% and 63.71% xylose consumption.展开更多
The aim of the study was to describe the binding of cholic acid by hemicellulose and pectin of yard-long bean at gastrointestinal system （in vitro）, through variation of acidity （pH 3, 5 and 7） and boiling time （...The aim of the study was to describe the binding of cholic acid by hemicellulose and pectin of yard-long bean at gastrointestinal system （in vitro）, through variation of acidity （pH 3, 5 and 7） and boiling time （raw/0, 5, 20 and 35 minutes） of yard-long bean. Two-way Anova （α= 5%） and functional groups analysis by infrared spectrophotometer were applied for the binding description. The results of the study showed： （1） The highest binding percentage of cholic acid by dietary fiber of yard-long bean at pH 3-raw treatment （76.603%）; （2） Cholic acid binding via C = O/ester or acid of hemicellulose, C-O-C/cyclic ether of pectin, while C = O/ester or acid of oectin through iron.展开更多
Objective： To assess the outcome of immediate plate osteosynthesis via application of antibiotic impregnated collagen fleeces （gentamicin-collagen and an- tibiotic sponge） which gradually release antibiotic locally...Objective： To assess the outcome of immediate plate osteosynthesis via application of antibiotic impregnated collagen fleeces （gentamicin-collagen and an- tibiotic sponge） which gradually release antibiotic locally in the surgical treatment of open fractures presented to us 6 hours after injury. Methods： All cases were treated in our tertiary level trauma center and teaching hospital including 35 patients with open fractures who were treated by immediate open reduction and plate fixation from January 2008 to August 2010. Among them, 31 patients were available for adequate follow-up and assessment. All fractures were treated by irri- gation and debridement, immediate open reduction and plate fixation along with placement of antibiotic-releasing collagen fleeces around the plate just before closure of wound. Pa- tients were assessed to determine postoperative infection, delayed union or nonunion and development of other post- operative complications. It was hypothesized that immedi- ate plate osteosynthesis after thorough debridement and local antibiotics would give safe and acceptable clinical results in treatment of open fractures. Results： The 31 patients with adequate final follow-up were assessed at a mean time of 40 weeks （15-160 weeks）.Most fractures united primarily in an acceptable time period according to area of involvement. Local wound complica- tions （superficial infection and skin loss） were found in 3 patients （9.67%）. Deep infection was noted in 2 patients （6.45%）. None of these patients needed implant removal and both fractures united in due time. Delayed union was noted in 5 patients （16.13%）. No patient progressed to non- union or implant failure in long term follow-up. Excessive scarring was developed in 2 patients （6.45%）. Conclusions： Immediate plate osteosynthesis after adequate debridement and placement of collagen film eluting antibiotics locally produces excellent results regarding bone union and absence of deep infections and is a safe technique in the management of open bone injuries. These sponges can be used easily with any form of internal fixa- tion and there is no need of second surgery for the removal of these antibiotic carriers since they are bioabsorbable. Local antibiotic-impregnated collagen sponges along with systemic antibiotics for 3 to 5 days offer promising results in open fracture management.展开更多
基金Project supported by the National Natural Science Foundation of China No: 39670837
文摘Objective: To study the antifibrotic effects of genistein(GE) and quercetin(QU) on rat hepatic stellate HSC-T6 cell proliferation stimulated with platelet-derived growth factor (PDGF), collagen synthesis and type I procollagen messenger RNA (mRNA) expression stimulated with transforming growth factor b1 (TGFb1). Methods: Cell proliferation was measured by crystal violet staining assay. Collagen synthesis was determined by 3H-proline incorporation assay. Type I procollagen mRNA level was determined by reverse transcription polymerase chain reaction (RT-PCR). Results: GE (25~70 mmolL-1) and QU (6.25~50 mmolL-1) concentration-dependently attenuated PDGF-driven HSC-T6 cell proliferative activity. TGFb1-stimulated collagen synthesis was also reduced. This was associated with a decrease in type I procollagen mRNA expression, indicating an effect at a pretranslational level. Conclusion: GE and QU may have therapeutic potential against liver fibrosis by regulating PDGF and TGFb1 actions.
基金Supported by the Agriculture Application Investigation and Improvement Item of New Countryside Construction and Promotion Project of the Bureau of Science and Technology in Xi’an (NC08005)
文摘Pretreatment of the corn stover powder by dilute sulphuric acid （solid-liquid ratio 1 ： 20） at 130 for 30 min was carried out with 89.09% of the hemicellulose removed. After filtration, the xylose-rich corn stover pretreatment liquid, whose fermentable sugar was from hemicellulose hydrolysis only, consisting of 81.16% xylose and 15.27% glucose, was used to cultivate genetic recombinant Escherichia coli BL21 with human-like collagen （HLC） expression enhanced by 50.00% and 63.71% xylose consumption.
文摘The aim of the study was to describe the binding of cholic acid by hemicellulose and pectin of yard-long bean at gastrointestinal system （in vitro）, through variation of acidity （pH 3, 5 and 7） and boiling time （raw/0, 5, 20 and 35 minutes） of yard-long bean. Two-way Anova （α= 5%） and functional groups analysis by infrared spectrophotometer were applied for the binding description. The results of the study showed： （1） The highest binding percentage of cholic acid by dietary fiber of yard-long bean at pH 3-raw treatment （76.603%）; （2） Cholic acid binding via C = O/ester or acid of hemicellulose, C-O-C/cyclic ether of pectin, while C = O/ester or acid of oectin through iron.
文摘Objective： To assess the outcome of immediate plate osteosynthesis via application of antibiotic impregnated collagen fleeces （gentamicin-collagen and an- tibiotic sponge） which gradually release antibiotic locally in the surgical treatment of open fractures presented to us 6 hours after injury. Methods： All cases were treated in our tertiary level trauma center and teaching hospital including 35 patients with open fractures who were treated by immediate open reduction and plate fixation from January 2008 to August 2010. Among them, 31 patients were available for adequate follow-up and assessment. All fractures were treated by irri- gation and debridement, immediate open reduction and plate fixation along with placement of antibiotic-releasing collagen fleeces around the plate just before closure of wound. Pa- tients were assessed to determine postoperative infection, delayed union or nonunion and development of other post- operative complications. It was hypothesized that immedi- ate plate osteosynthesis after thorough debridement and local antibiotics would give safe and acceptable clinical results in treatment of open fractures. Results： The 31 patients with adequate final follow-up were assessed at a mean time of 40 weeks （15-160 weeks）.Most fractures united primarily in an acceptable time period according to area of involvement. Local wound complica- tions （superficial infection and skin loss） were found in 3 patients （9.67%）. Deep infection was noted in 2 patients （6.45%）. None of these patients needed implant removal and both fractures united in due time. Delayed union was noted in 5 patients （16.13%）. No patient progressed to non- union or implant failure in long term follow-up. Excessive scarring was developed in 2 patients （6.45%）. Conclusions： Immediate plate osteosynthesis after adequate debridement and placement of collagen film eluting antibiotics locally produces excellent results regarding bone union and absence of deep infections and is a safe technique in the management of open bone injuries. These sponges can be used easily with any form of internal fixa- tion and there is no need of second surgery for the removal of these antibiotic carriers since they are bioabsorbable. Local antibiotic-impregnated collagen sponges along with systemic antibiotics for 3 to 5 days offer promising results in open fracture management.
