为考察羧甲基果聚糖及其衍生物在功能性食品、生物医学及化妆品方面的应用可行性,通过羧基与氨基的交联反应制备了胶原肽修饰的羧甲基果聚糖,并测试了产物的抗氧化、吸湿保湿活性以及其对人皮角质Ha Ca T细胞生长增殖的影响。发现羧甲...为考察羧甲基果聚糖及其衍生物在功能性食品、生物医学及化妆品方面的应用可行性,通过羧基与氨基的交联反应制备了胶原肽修饰的羧甲基果聚糖,并测试了产物的抗氧化、吸湿保湿活性以及其对人皮角质Ha Ca T细胞生长增殖的影响。发现羧甲基果聚糖经胶原肽修饰后抗氧化性明显提高,25 mg/m L时的ABTS^+·清除率为62.31%,比修饰前提高了6.11倍。尽管胶原肽的引入会对吸湿保湿性造成影响,修饰后的羧甲基果聚糖仍然保留良好的吸湿保湿性能。在相对湿度43%和81%中放置72 h的吸湿率分别为11.96%和21.54%,在干燥硅胶环境中放置72 h的保湿率为60.48%。此外,修饰产物在0~1 mg/m L的浓度范围内无细胞毒性,可以促进Ha Ca T细胞增殖。Ha Ca T细胞在0.5 mg/m L修饰产物刺激下的存活率为124.2%。胶原肽修饰能提高羧甲基果聚糖的生物活性,拓展其在功能性食品、医药制品和化妆品上的应用潜力。展开更多
Characteristics and antioxidant activities of pepsin-soluble collagen （PSC） from yellow goosefish （Lophius litulon） skins were investigated. PSC was characterized as a type I collagen, and its imino acid content w...Characteristics and antioxidant activities of pepsin-soluble collagen （PSC） from yellow goosefish （Lophius litulon） skins were investigated. PSC was characterized as a type I collagen, and its imino acid content was 193 residues/1 000 residues. PSC＇s denaturation temperature was -17.56℃ and Fourier transform infrared spectra confirmed the presence of triple helices. Solubility analysis showed good solubility at acidic pH （1-6） or low NaCl concentrations （≤2%）. PSC showed scavenging activity against hydroxyl radicals and superoxide anions in a concentration-dependent manner. Furthermore, PSC could protect D-galactose-induced skin aging by significantly controlling malondialdehyde formation and improving the activity of superoxide dismutase, glutathione peroxidase, catalase, glutathione, and hydroxyproline. PSC may be a promising antioxidant in appropriate applications.展开更多
This study compares the persistence and histological characteristics of gut suture with those of human amnion collagen, bovine collagen, and Vicryl suture implants in rats. Gut suture and human amnion collagen more re...This study compares the persistence and histological characteristics of gut suture with those of human amnion collagen, bovine collagen, and Vicryl suture implants in rats. Gut suture and human amnion collagen more resembled living tissue than did bovine collagen and were characterized by their cel1ularity and the presence of numerous capillaries. The Vicryl suture implants were quickly absorbed. Picrosirius polarization revealed the synthesis of host collagen by rat fibroblasts which immigrated into the gut suture and human amnion collagen implants. The authors suggest the potential of gut suture as a soft tissue substitute to improve linear skin contour deficits.展开更多
文摘为考察羧甲基果聚糖及其衍生物在功能性食品、生物医学及化妆品方面的应用可行性,通过羧基与氨基的交联反应制备了胶原肽修饰的羧甲基果聚糖,并测试了产物的抗氧化、吸湿保湿活性以及其对人皮角质Ha Ca T细胞生长增殖的影响。发现羧甲基果聚糖经胶原肽修饰后抗氧化性明显提高,25 mg/m L时的ABTS^+·清除率为62.31%,比修饰前提高了6.11倍。尽管胶原肽的引入会对吸湿保湿性造成影响,修饰后的羧甲基果聚糖仍然保留良好的吸湿保湿性能。在相对湿度43%和81%中放置72 h的吸湿率分别为11.96%和21.54%,在干燥硅胶环境中放置72 h的保湿率为60.48%。此外,修饰产物在0~1 mg/m L的浓度范围内无细胞毒性,可以促进Ha Ca T细胞增殖。Ha Ca T细胞在0.5 mg/m L修饰产物刺激下的存活率为124.2%。胶原肽修饰能提高羧甲基果聚糖的生物活性,拓展其在功能性食品、医药制品和化妆品上的应用潜力。
基金Supported by the International S&T Cooperation Program of China(No.2015DFA30980)the Zhejiang Provincial Natural Science Foundation of China(No.LQ14C170001)the Special Program for the Science and Technology Plan of Zhejiang Province(No.2011C02003)
文摘Characteristics and antioxidant activities of pepsin-soluble collagen （PSC） from yellow goosefish （Lophius litulon） skins were investigated. PSC was characterized as a type I collagen, and its imino acid content was 193 residues/1 000 residues. PSC＇s denaturation temperature was -17.56℃ and Fourier transform infrared spectra confirmed the presence of triple helices. Solubility analysis showed good solubility at acidic pH （1-6） or low NaCl concentrations （≤2%）. PSC showed scavenging activity against hydroxyl radicals and superoxide anions in a concentration-dependent manner. Furthermore, PSC could protect D-galactose-induced skin aging by significantly controlling malondialdehyde formation and improving the activity of superoxide dismutase, glutathione peroxidase, catalase, glutathione, and hydroxyproline. PSC may be a promising antioxidant in appropriate applications.
文摘This study compares the persistence and histological characteristics of gut suture with those of human amnion collagen, bovine collagen, and Vicryl suture implants in rats. Gut suture and human amnion collagen more resembled living tissue than did bovine collagen and were characterized by their cel1ularity and the presence of numerous capillaries. The Vicryl suture implants were quickly absorbed. Picrosirius polarization revealed the synthesis of host collagen by rat fibroblasts which immigrated into the gut suture and human amnion collagen implants. The authors suggest the potential of gut suture as a soft tissue substitute to improve linear skin contour deficits.
